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The map label for this gene is topA

Identifier: 82750851

GI number: 82750851

Start: 1202213

End: 1204288

Strand: Direct

Name: topA

Synonym: SAB1112

Alternate gene names: 82750851

Gene position: 1202213-1204288 (Clockwise)

Preceding gene: 82750850

Following gene: 82750852

Centisome position: 43.84

GC content: 34.78

Gene sequence:

>2076_bases
ATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATA
TAAAGTTATAGCTTCAATGGGACACGTCAGAGACTTACCAAGAAGTCAAATGGGTGTCGACACTGAAGATAATTACGAAC
CAAAATATATAACAATACGCGGAAAAGGTCCTGTTGTAAAAGAATTGAAAAAACATGCAAAAAAAGCGAAAAACGTCTTT
CTCGCAAGTGACCCCGACCGTGAAGGTGAAGCAATTGCTTGGCATTTATCAAAAATTTTAGAGCTTGAAGATTCTAAAGA
AAATCGCGTTGTTTTCAACGAAATAACTAAAGACGCTGTTAAAGAAAGTTTTAAAAATCCTAGAGAAATTGAAATGAACT
TAGTCGATGCACAACAAGCGCGTCGAATATTAGATAGATTAGTTGGCTATAACATCTCGCCAGTTCTTTGGAAAAAAGTA
AAAAAAGGGTTGTCAGCGGGTCGAGTTCAATCTGTTGCACTTCGTTTAGTCATTGACCGTGAAAATGAAATTCGAAACTT
TAAACCAGAAGAATATTGGATTATTGAAGGAGAATTTAGATACAAAAAATCAAAATTCAATGCTAAATTCCTTCATTATA
AAAATAAACCTTTTAAACTAAAAACGAAAAAAGATATTGAGAAAATTACAGCTGCATTAGATGGAGATCAATTCGAAATT
ACAAACGTGACTAAAAAAGAAAAAACGCGTAATCCAGCAAACCCATTTACAACTTCTACATTACAACAAGAGGCGGCACG
TAAATTAAACTTTAAAGCTAGAAAAACAATGATGGTCGCACAACAATTATATGAAGGTATAGATTTGAAAAAACAAGGTA
CGATTGGTTTAATAACATATATGAGAACCGATTCTACACGTATTTCAGATACTGCCAAAGCTGAAGCAAAACAGTATATA
ACTGATAAATACGGTGAATCTTACACTTCTAAACGTAAAGCATCAGGGAAACAAGGTGACCAAGATGCCCATGAGGCTAT
TAGACCTTCAAGTACTATGCGTACGCCAGATGATATGAAGTCATTTTTGACGAAAGACCAATATCGATTATACAAATTAA
TTTGGGAACGATTTGTTGCTAGTCAAATGGCTCCAGCAATACTTGATACAGTCTCATTAGATATAACACAAGGTGACATT
AAATTTAGAGCGAATGGTCAAACAATCAAGTTTAAAGGATTTATGACACTTTATGTTGAAACTAAAGATGATATTGATAG
CGAAAAGGAAAATAAACTGCCTAAATTAGAGCAAGGTGATAAAGTCACAGCAACTCAAATTGAACCAGCTCAACACTATA
CACAACCACCTCCAAGATATACTGAGGCGAGATTAGTAAAAACACTAGAAGAATTGAAAATTGGGCGACCATCAACTTAT
GCACCGACAATAGATACGATTCAAAAGCGTAACTATGTCAAATTAGAAAGTAAGCGTTTTGTTCCTACTGAGTTGGGAGA
AATAGTTCATGAACAAGTGAAAGAATACTTCCCAGAGATTATTGATGTGGAATTCACAGTGAATATGGAAACGTTACTTG
ATAAGATTGCAGAAGGCGACATTACATGGAGAAAAGTAATCGACGGTTTCTTTAGTAGCTTTAAACAAGATGTTGAACGT
GCTGAAGAAGAGATGGAAAAGATTGAAATCAAAGATGAGCCAGCCGGTGAAGACTGTGAAGTTTGTGGTTCTCCTATGGT
TATAAAAATGGGACGCTATGGTAAGTTTATGGCTTGTTCAAACTTCCCGGATTGTCGTAATACAAAAGCGATAGTTAAAT
CTATTGGTGTTAAATGTCCAAAATGTAATGATGGCGACGTCGTAGAAAGAAAATCTAAAAAGAATCGTGTCTTTTATGGA
TGTTCGAAATATCCTGAATGCGACTTTATCTCTTGGGATAAGCCGATTGGAAGAGATTGTCCAAAATGTAACCAATATCT
TGTTGAAAATAAAAAAGGCAAGACAACACAAGTAATATGTTCAAATTGCGATTATAAAGAGGCAGCGCAGAAATAA

Upstream 100 bases:

>100_bases
AAATAGACAAGGAAAATGATTTGTTTAGATATAAAACGTTGACAAAAGCAAAATTAAGCGTTTATCATTTATCTTTAGTA
ATTAGATTAGCGAGGGGGAA

Downstream 100 bases:

>100_bases
TATTTTTATTTCCTAGAGACATTTTAAGATTGTTAAATAGAATCATTAGTGAATTTTATTTTAAAGATAGTAATGGATTA
ATCTAAATAAGTGCGGATAA

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 691; Mature: 690

Protein sequence:

>691_residues
MTLADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQMGVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVF
LASDPDREGEAIAWHLSKILELEDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKV
KKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWIIEGEFRYKKSKFNAKFLHYKNKPFKLKTKKDIEKITAALDGDQFEI
TNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKTMMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKAEAKQYI
TDKYGESYTSKRKASGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVSLDITQGDI
KFRANGQTIKFKGFMTLYVETKDDIDSEKENKLPKLEQGDKVTATQIEPAQHYTQPPPRYTEARLVKTLEELKIGRPSTY
APTIDTIQKRNYVKLESKRFVPTELGEIVHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVER
AEEEMEKIEIKDEPAGEDCEVCGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCNDGDVVERKSKKNRVFYG
CSKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSNCDYKEAAQK

Sequences:

>Translated_691_residues
MTLADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQMGVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVF
LASDPDREGEAIAWHLSKILELEDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKV
KKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWIIEGEFRYKKSKFNAKFLHYKNKPFKLKTKKDIEKITAALDGDQFEI
TNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKTMMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKAEAKQYI
TDKYGESYTSKRKASGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVSLDITQGDI
KFRANGQTIKFKGFMTLYVETKDDIDSEKENKLPKLEQGDKVTATQIEPAQHYTQPPPRYTEARLVKTLEELKIGRPSTY
APTIDTIQKRNYVKLESKRFVPTELGEIVHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVER
AEEEMEKIEIKDEPAGEDCEVCGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCNDGDVVERKSKKNRVFYG
CSKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSNCDYKEAAQK
>Mature_690_residues
TLADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQMGVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFL
ASDPDREGEAIAWHLSKILELEDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKVK
KGLSAGRVQSVALRLVIDRENEIRNFKPEEYWIIEGEFRYKKSKFNAKFLHYKNKPFKLKTKKDIEKITAALDGDQFEIT
NVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKTMMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKAEAKQYIT
DKYGESYTSKRKASGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVSLDITQGDIK
FRANGQTIKFKGFMTLYVETKDDIDSEKENKLPKLEQGDKVTATQIEPAQHYTQPPPRYTEARLVKTLEELKIGRPSTYA
PTIDTIQKRNYVKLESKRFVPTELGEIVHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERA
EEEMEKIEIKDEPAGEDCEVCGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCNDGDVVERKSKKNRVFYGC
SKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSNCDYKEAAQK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=630, Percent_Identity=25.5555555555556, Blast_Score=139, Evalue=8e-33,
Organism=Homo sapiens, GI4507635, Length=556, Percent_Identity=24.1007194244604, Blast_Score=123, Evalue=5e-28,
Organism=Escherichia coli, GI1787529, Length=753, Percent_Identity=38.7782204515272, Blast_Score=520, Evalue=1e-148,
Organism=Escherichia coli, GI1788061, Length=632, Percent_Identity=22.4683544303797, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI48994931, Length=110, Percent_Identity=36.3636363636364, Blast_Score=100, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI32563869, Length=588, Percent_Identity=25.6802721088435, Blast_Score=137, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17555378, Length=506, Percent_Identity=23.5177865612648, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6323263, Length=503, Percent_Identity=23.6580516898608, Blast_Score=117, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24585251, Length=629, Percent_Identity=23.2114467408585, Blast_Score=143, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24640096, Length=519, Percent_Identity=23.121387283237, Blast_Score=103, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 79338; Mature: 79207

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQMGVDTEDNYEPKYITIR
CCCCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCEEEEE
GKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILELEDSKENRVVFNEITKDAV
CCCCHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
KESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKVKKGLSAGRVQSVALRLVIDR
HHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHEEEEEEECC
ENEIRNFKPEEYWIIEGEFRYKKSKFNAKFLHYKNKPFKLKTKKDIEKITAALDGDQFEI
CHHHCCCCCCEEEEEECCEEEHHHHCCEEEEEECCCCEEECCHHHHHHHHHHHCCCCEEE
TNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKTMMVAQQLYEGIDLKKQGTIGLITY
ECCHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
MRTDSTRISDTAKAEAKQYITDKYGESYTSKRKASGKQGDQDAHEAIRPSSTMRTPDDMK
EECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHCCCCCCCCCHHHHH
SFLTKDQYRLYKLIWERFVASQMAPAILDTVSLDITQGDIKFRANGQTIKFKGFMTLYVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCEEEEECCCEEEEEEEEEEEEE
TKDDIDSEKENKLPKLEQGDKVTATQIEPAQHYTQPPPRYTEARLVKTLEELKIGRPSTY
CCCCCCCHHHHCCCCCCCCCCEEEEECCHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCC
APTIDTIQKRNYVKLESKRFVPTELGEIVHEQVKEYFPEIIDVEFTVNMETLLDKIAEGD
CCCHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHCHHHEEEEEEECHHHHHHHHHCCC
ITWRKVIDGFFSSFKQDVERAEEEMEKIEIKDEPAGEDCEVCGSPMVIKMGRYGKFMACS
CHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHCCCCEEEEECCCCCEEEEC
NFPDCRNTKAIVKSIGVKCPKCNDGDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDC
CCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCC
PKCNQYLVENKKGKTTQVICSNCDYKEAAQK
CHHHHHHHCCCCCCEEEEEECCCCHHHHHCC
>Mature Secondary Structure 
TLADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQMGVDTEDNYEPKYITIR
CCCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCEEEEE
GKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILELEDSKENRVVFNEITKDAV
CCCCHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
KESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKVKKGLSAGRVQSVALRLVIDR
HHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHEEEEEEECC
ENEIRNFKPEEYWIIEGEFRYKKSKFNAKFLHYKNKPFKLKTKKDIEKITAALDGDQFEI
CHHHCCCCCCEEEEEECCEEEHHHHCCEEEEEECCCCEEECCHHHHHHHHHHHCCCCEEE
TNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKTMMVAQQLYEGIDLKKQGTIGLITY
ECCHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
MRTDSTRISDTAKAEAKQYITDKYGESYTSKRKASGKQGDQDAHEAIRPSSTMRTPDDMK
EECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHCCCCCCCCCHHHHH
SFLTKDQYRLYKLIWERFVASQMAPAILDTVSLDITQGDIKFRANGQTIKFKGFMTLYVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCEEEEECCCEEEEEEEEEEEEE
TKDDIDSEKENKLPKLEQGDKVTATQIEPAQHYTQPPPRYTEARLVKTLEELKIGRPSTY
CCCCCCCHHHHCCCCCCCCCCEEEEECCHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCC
APTIDTIQKRNYVKLESKRFVPTELGEIVHEQVKEYFPEIIDVEFTVNMETLLDKIAEGD
CCCHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHCHHHEEEEEEECHHHHHHHHHCCC
ITWRKVIDGFFSSFKQDVERAEEEMEKIEIKDEPAGEDCEVCGSPMVIKMGRYGKFMACS
CHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHCCCCEEEEECCCCCEEEEC
NFPDCRNTKAIVKSIGVKCPKCNDGDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDC
CCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCC
PKCNQYLVENKKGKTTQVICSNCDYKEAAQK
CHHHHHHHCCCCCCEEEEEECCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA