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The map label for this gene is rlmN [H]

Identifier: 82750822

GI number: 82750822

Start: 1168139

End: 1169233

Strand: Direct

Name: rlmN [H]

Synonym: SAB1082

Alternate gene names: 82750822

Gene position: 1168139-1169233 (Clockwise)

Preceding gene: 82750821

Following gene: 82750823

Centisome position: 42.59

GC content: 33.61

Gene sequence:

>1095_bases
ATGATAACTGCTGAAAAGAAAAAGAAGAATAAATTTCTTCCAAATTTTGACAAGCAATCAATATATTCATTGCGATTTGA
CGAAATGCAAAACTGGCTCGTTGAACAAGGTCAACAAAAATTTCGAGCGAAACAGATTTTTGAATGGTTATATCAAAAAA
GAGTAGATTCGATTGATGAAATGACGAACTTATCGAAAGACTTACGACAGCTTTTAAAAGATAACTTTACTGTTACAACT
TTAACAACTGTAGTAAAACAAGAAAGTAAAGACGGTACAATTAAATTCTTATTTGAATTACAAGATGGCTATACAATTGA
AACTGTTTTAATGAGACATGATTATGGAAATTCAGTATGTGTAACGACACAAGTAGGTTGTCGTATCGGATGTACGTTTT
GTGCTTCTACACTTGGCGGTTTAAAAAGAAACCTTGAAGCTGGCGAAATTGTTTCACAAGTTTTAACAGTTCAAAAAGCC
CTTGATGCTACAGAAGAGCGTGTATCTCAAATTGTCATAATGGGTATCGGTGAACCATTTGAAAATTATGATGAAATGAT
GGGCTTTTTAAGAATCGTCAATGATGATAACAGTTTAAATATTGGTGCACGTCACATTACAGTATCAACATCAGGTATCA
TTCCTAGAATATATGACTTTGCGGATGAAGATATCCAAATTAATTTTGCTGTAAGCTTACACGCCGCAAAAGATGAAGTG
CGATCACGCTTGATGCCAATTAACCGTGCATATAATGTTGAGAAGTTAATCGAAGCAATTCAATATTATCAAGAAAAAAC
AAATCGTCGTGTTACTTTTGAATATGGTCTGTTTGGTGGTGTGAATGACCAACTAGAACATGCAAGAGAATTAGCACATT
TAATAAAAGGCTTAAACTGCCATGTTAACTTAATTCCTGTCAACCATGTTCCAGAAAGAAATTATGTAAAAACGGCTAAA
AATGATATCTTTAAATTTGAAAAAGAATTAAAGAGACTAGGAATTAATGCCACAATACGTCGTGAACAAGGTTCAGATAT
TGACGCAGCTTGTGGTCAATTAAGAGCAAAGGAACGACAAGTAGAAACGAGGTAA

Upstream 100 bases:

>100_bases
CGATAACTGGAGAGTTGGTCAAAACGTTACAAATCATGCCGCAAGACTTTAATTCAGATGGATTCTTTATCACTAAGATA
AAAAGAAAGGACAATTAGGA

Downstream 100 bases:

>100_bases
AGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGATGCGGGTGGTATTTTTTA
TAATCAAACTAATCAACAAC

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAKQIFEWLYQKRVDSIDEMTNLSKDLRQLLKDNFTVTT
LTTVVKQESKDGTIKFLFELQDGYTIETVLMRHDYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKA
LDATEERVSQIVIMGIGEPFENYDEMMGFLRIVNDDNSLNIGARHITVSTSGIIPRIYDFADEDIQINFAVSLHAAKDEV
RSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYGLFGGVNDQLEHARELAHLIKGLNCHVNLIPVNHVPERNYVKTAK
NDIFKFEKELKRLGINATIRREQGSDIDAACGQLRAKERQVETR

Sequences:

>Translated_364_residues
MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAKQIFEWLYQKRVDSIDEMTNLSKDLRQLLKDNFTVTT
LTTVVKQESKDGTIKFLFELQDGYTIETVLMRHDYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKA
LDATEERVSQIVIMGIGEPFENYDEMMGFLRIVNDDNSLNIGARHITVSTSGIIPRIYDFADEDIQINFAVSLHAAKDEV
RSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYGLFGGVNDQLEHARELAHLIKGLNCHVNLIPVNHVPERNYVKTAK
NDIFKFEKELKRLGINATIRREQGSDIDAACGQLRAKERQVETR
>Mature_364_residues
MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAKQIFEWLYQKRVDSIDEMTNLSKDLRQLLKDNFTVTT
LTTVVKQESKDGTIKFLFELQDGYTIETVLMRHDYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKA
LDATEERVSQIVIMGIGEPFENYDEMMGFLRIVNDDNSLNIGARHITVSTSGIIPRIYDFADEDIQINFAVSLHAAKDEV
RSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYGLFGGVNDQLEHARELAHLIKGLNCHVNLIPVNHVPERNYVKTAK
NDIFKFEKELKRLGINATIRREQGSDIDAACGQLRAKERQVETR

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA. Confers resistance to some classes of antibiotics [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=344, Percent_Identity=37.5, Blast_Score=251, Evalue=6e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 41847; Mature: 41847

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAKQIFEWLYQKRVDSIDE
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MTNLSKDLRQLLKDNFTVTTLTTVVKQESKDGTIKFLFELQDGYTIETVLMRHDYGNSVC
HHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEEEEECCCCCEEE
VTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKALDATEERVSQIVIMGIGEPF
EEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCC
ENYDEMMGFLRIVNDDNSLNIGARHITVSTSGIIPRIYDFADEDIQINFAVSLHAAKDEV
CHHHHHHHHHHHCCCCCCEEECEEEEEEECCCCCHHHHHCCCCCEEEEEEEEEEHHHHHH
RSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYGLFGGVNDQLEHARELAHLIKGLNC
HHHHCCCHHCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCC
HVNLIPVNHVPERNYVKTAKNDIFKFEKELKRLGINATIRREQGSDIDAACGQLRAKERQ
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHH
VETR
CCCC
>Mature Secondary Structure
MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAKQIFEWLYQKRVDSIDE
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MTNLSKDLRQLLKDNFTVTTLTTVVKQESKDGTIKFLFELQDGYTIETVLMRHDYGNSVC
HHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEEEEECCCCCEEE
VTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKALDATEERVSQIVIMGIGEPF
EEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCC
ENYDEMMGFLRIVNDDNSLNIGARHITVSTSGIIPRIYDFADEDIQINFAVSLHAAKDEV
CHHHHHHHHHHHCCCCCCEEECEEEEEEECCCCCHHHHHCCCCCEEEEEEEEEEHHHHHH
RSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYGLFGGVNDQLEHARELAHLIKGLNC
HHHHCCCHHCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCC
HVNLIPVNHVPERNYVKTAKNDIFKFEKELKRLGINATIRREQGSDIDAACGQLRAKERQ
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHH
VETR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA