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The map label for this gene is nifU [H]

Identifier: 82750527

GI number: 82750527

Start: 846053

End: 846517

Strand: Direct

Name: nifU [H]

Synonym: SAB0777

Alternate gene names: 82750527

Gene position: 846053-846517 (Clockwise)

Preceding gene: 82750526

Following gene: 82750528

Centisome position: 30.85

GC content: 36.13

Gene sequence:

>465_bases
ATGAATTTTAATAATCTAGATCAATTATATAGATCTGTCATTATGGATCATTATAAAAATCCTAGAAATAAAGGTGTATT
AGATAACGGGTCTATGACAGTAGATATGAATAACCCGACATGCGGTGACCGTATACGACTAACATTTGATATAGAAGACG
GCATTATAAAAGATGCTAAGTTTGAAGGTGAAGGTTGTTCGATTTCAATGGCAAGTGCATCGATGATGACACAAGCTGTT
AAAGGTCATTCACTTGGAGAAGCAATGCAAATGAGCCAAGAATTTACGAAAATGATGCTTGGTGAAGACTATGTGATTAC
AGAAGAAATGGGAGATATTGAAGCATTGCAAGGTGTATCTCAATTCCCAGCTCGTATTAAATGTGCCACATTAGCTTGGA
AAGCATTGGAAAAAGGTACTGTTGCTAAAGAAGGTAAAGCAGAAGGTACGACTGAAGAAGAATAG

Upstream 100 bases:

>100_bases
GTGTCTTCAACAGCTAGAGCGAGTTTTTATATATACAACACGAAAGAAGACATTGATCAGTTAATAAATGCCTTGAAACA
AACGAAGGAGTTTTTCTCTT

Downstream 100 bases:

>100_bases
AATGCTGTTAATCATAGATGATTTTGATATTAGACATATAAAAGTATAAAATTTTTTATAAGATGTCATGTCATTGTTAT
AATATGGTTTACATCATGAA

Product: nitrogen fixation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MNFNNLDQLYRSVIMDHYKNPRNKGVLDNGSMTVDMNNPTCGDRIRLTFDIEDGIIKDAKFEGEGCSISMASASMMTQAV
KGHSLGEAMQMSQEFTKMMLGEDYVITEEMGDIEALQGVSQFPARIKCATLAWKALEKGTVAKEGKAEGTTEEE

Sequences:

>Translated_154_residues
MNFNNLDQLYRSVIMDHYKNPRNKGVLDNGSMTVDMNNPTCGDRIRLTFDIEDGIIKDAKFEGEGCSISMASASMMTQAV
KGHSLGEAMQMSQEFTKMMLGEDYVITEEMGDIEALQGVSQFPARIKCATLAWKALEKGTVAKEGKAEGTTEEE
>Mature_154_residues
MNFNNLDQLYRSVIMDHYKNPRNKGVLDNGSMTVDMNNPTCGDRIRLTFDIEDGIIKDAKFEGEGCSISMASASMMTQAV
KGHSLGEAMQMSQEFTKMMLGEDYVITEEMGDIEALQGVSQFPARIKCATLAWKALEKGTVAKEGKAEGTTEEE

Specific function: May be involved in the formation or repair of [Fe-S] clusters present in iron-sulfur proteins (Potential) [H]

COG id: COG0822

COG function: function code C; NifU homolog involved in Fe-S cluster formation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifU family [H]

Homologues:

Organism=Homo sapiens, GI56699456, Length=88, Percent_Identity=40.9090909090909, Blast_Score=71, Evalue=3e-13,
Organism=Homo sapiens, GI24307953, Length=82, Percent_Identity=41.4634146341463, Blast_Score=67, Evalue=8e-12,
Organism=Escherichia coli, GI1788878, Length=144, Percent_Identity=33.3333333333333, Blast_Score=76, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324800, Length=135, Percent_Identity=32.5925925925926, Blast_Score=75, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6325122, Length=135, Percent_Identity=30.3703703703704, Blast_Score=68, Evalue=5e-13,
Organism=Drosophila melanogaster, GI21355597, Length=87, Percent_Identity=42.5287356321839, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002871
- InterPro:   IPR011341 [H]

Pfam domain/function: PF01592 NifU_N [H]

EC number: NA

Molecular weight: Translated: 17017; Mature: 17017

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
7.8 %Met     (Translated Protein)
9.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
7.8 %Met     (Mature Protein)
9.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFNNLDQLYRSVIMDHYKNPRNKGVLDNGSMTVDMNNPTCGDRIRLTFDIEDGIIKDAK
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCEEEEEEECCCCEECCCE
FEGEGCSISMASASMMTQAVKGHSLGEAMQMSQEFTKMMLGEDYVITEEMGDIEALQGVS
ECCCCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHH
QFPARIKCATLAWKALEKGTVAKEGKAEGTTEEE
HCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNFNNLDQLYRSVIMDHYKNPRNKGVLDNGSMTVDMNNPTCGDRIRLTFDIEDGIIKDAK
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCEEEEEEECCCCEECCCE
FEGEGCSISMASASMMTQAVKGHSLGEAMQMSQEFTKMMLGEDYVITEEMGDIEALQGVS
ECCCCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHH
QFPARIKCATLAWKALEKGTVAKEGKAEGTTEEE
HCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]