Click here to switch to the map view.

The map label for this gene is nfrA [H]

Identifier: 82750093

GI number: 82750093

Start: 377827

End: 378582

Strand: Direct

Name: nfrA [H]

Synonym: SAB0332

Alternate gene names: 82750093

Gene position: 377827-378582 (Clockwise)

Preceding gene: 82750087

Following gene: 82750099

Centisome position: 13.78

GC content: 37.7

Gene sequence:

>756_bases
GTGTCAGATTATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGAAAATTTAAGAATAAACCTTTAAGTGAAGACGT
TGTTAAGAAATTGGTAGAAGCTGGACAAAGCGCTTCGACGTCAAGTTTCCTACAAGCATACTCAATTATTGGTATCGACG
ATGAGAAGATTAAAGAAAATTTACGAGAAGTTTCTGGTCAACCTTATGTTGTAGAAAATGGCTATTTATTCATCTTTGTT
ATTGATTATTATCGTCATCATTTAGTTGATCAACATGCTGAGACTGATATGGAAAATGCATATGGTTCAACGGAAGGTTT
GCTAGTAGGTGCAATCGATGCAGCATTAGTTGCCGAAAATATTGCGGTAACTGCTGAAGATATGGGGTATGGCATTGTCT
TTTTAGGATCATTAAGAAATGATGTTGAACGCGTTCGAGAAATTTTAGACTTACCTGACTATGTCTTCCCGGTATTTGGT
ATGGCAGTAGGGGAACCCGCAGATGACGAAAATGGTGCAGCCAAGCCACGCTTACCATTTGACCATGTCTTCCATCATAA
TAAGTATCATGCTGATAAGGAAACACAGTATGCACAAATGGCAGATTACGATCAGACAATCAGCGAGTACTATGATCAAC
GTACAAACGGGAATCGCAAAGAAACATGGTCGCAGCAAATTGAGATGTTCCTAGGAAACAAAGCAAGATTAGATATGTTA
GAACAATTGCAAAAATCAGGCTTAATACAGCGATAG

Upstream 100 bases:

>100_bases
ATTAACTTATAAGCAAATAATTGCTATAAAAAAGTTTGGACGTGTACAATTGCAATATGAAGATTTTTAATTAACTGTAA
AGTATCGAGGAGTGGATAAC

Downstream 100 bases:

>100_bases
CAAGACACCAAAATAACCCGCCCCCTCTAGCTTAAAATGATAAGTATAGCTAGAGGGGGCGGGTATTTCTTGCAATGAAT
TAGTGTGAAGTTAATGCAGC

Product: nitro/flavin reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MSDYVYNLVKKHHSVRKFKNKPLSEDVVKKLVEAGQSASTSSFLQAYSIIGIDDEKIKENLREVSGQPYVVENGYLFIFV
IDYYRHHLVDQHAETDMENAYGSTEGLLVGAIDAALVAENIAVTAEDMGYGIVFLGSLRNDVERVREILDLPDYVFPVFG
MAVGEPADDENGAAKPRLPFDHVFHHNKYHADKETQYAQMADYDQTISEYYDQRTNGNRKETWSQQIEMFLGNKARLDML
EQLQKSGLIQR

Sequences:

>Translated_251_residues
MSDYVYNLVKKHHSVRKFKNKPLSEDVVKKLVEAGQSASTSSFLQAYSIIGIDDEKIKENLREVSGQPYVVENGYLFIFV
IDYYRHHLVDQHAETDMENAYGSTEGLLVGAIDAALVAENIAVTAEDMGYGIVFLGSLRNDVERVREILDLPDYVFPVFG
MAVGEPADDENGAAKPRLPFDHVFHHNKYHADKETQYAQMADYDQTISEYYDQRTNGNRKETWSQQIEMFLGNKARLDML
EQLQKSGLIQR
>Mature_250_residues
SDYVYNLVKKHHSVRKFKNKPLSEDVVKKLVEAGQSASTSSFLQAYSIIGIDDEKIKENLREVSGQPYVVENGYLFIFVI
DYYRHHLVDQHAETDMENAYGSTEGLLVGAIDAALVAENIAVTAEDMGYGIVFLGSLRNDVERVREILDLPDYVFPVFGM
AVGEPADDENGAAKPRLPFDHVFHHNKYHADKETQYAQMADYDQTISEYYDQRTNGNRKETWSQQIEMFLGNKARLDMLE
QLQKSGLIQR

Specific function: Reduces FMN, organic nitro compounds and disulfide DTNB. Involved in maintenance of the cellular redox state and the disulfide stress response [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=246, Percent_Identity=36.1788617886179, Blast_Score=153, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016446
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 28623; Mature: 28492

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDYVYNLVKKHHSVRKFKNKPLSEDVVKKLVEAGQSASTSSFLQAYSIIGIDDEKIKEN
CCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHH
LREVSGQPYVVENGYLFIFVIDYYRHHLVDQHAETDMENAYGSTEGLLVGAIDAALVAEN
HHHHCCCCEEEECCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHH
IAVTAEDMGYGIVFLGSLRNDVERVREILDLPDYVFPVFGMAVGEPADDENGAAKPRLPF
HEEEHHHCCCCEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCH
DHVFHHNKYHADKETQYAQMADYDQTISEYYDQRTNGNRKETWSQQIEMFLGNKARLDML
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHH
EQLQKSGLIQR
HHHHHCCCCCC
>Mature Secondary Structure 
SDYVYNLVKKHHSVRKFKNKPLSEDVVKKLVEAGQSASTSSFLQAYSIIGIDDEKIKEN
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHH
LREVSGQPYVVENGYLFIFVIDYYRHHLVDQHAETDMENAYGSTEGLLVGAIDAALVAEN
HHHHCCCCEEEECCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHH
IAVTAEDMGYGIVFLGSLRNDVERVREILDLPDYVFPVFGMAVGEPADDENGAAKPRLPF
HEEEHHHCCCCEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCH
DHVFHHNKYHADKETQYAQMADYDQTISEYYDQRTNGNRKETWSQQIEMFLGNKARLDML
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHH
EQLQKSGLIQR
HHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA