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The map label for this gene is gatD [H]

Identifier: 82749956

GI number: 82749956

Start: 235709

End: 236752

Strand: Direct

Name: gatD [H]

Synonym: SAB0189

Alternate gene names: 82749956

Gene position: 235709-236752 (Clockwise)

Preceding gene: 82749955

Following gene: 82749957

Centisome position: 8.59

GC content: 37.55

Gene sequence:

>1044_bases
GTGAAAGCTTTGAAATTATATGGCGTGGAAGATTTACGGTATGAGGATAATGATAAGCCAGTCATTGAAAGTGCGAATGA
CGTTATTGTTAAAGTGCGCGCAACGGGTATATGTGGTTCAGACACGTCACGATACAAAAAAATGGGGCCATACATTAAAG
GTATGCCATTTGGTCATGAATTTTCAGGTGTTGTAGATGCCATTGGAAGTGATGTTACGCATGTTAATGTAGGCGGCAAA
GTGACAGGTTGCCCAGCAATACCTTGTTATCAATGCGAGTATTGTTTAAAAGGTGAATATGCACGATGTAAAAAGTTATT
CGTCATTGGCTCATATGAACCTGGATCGTTCGCGGAATATGTCAAATTGCCAGCACAAAATGTTTTAAAGGTTCCAGACA
ATGTTGATTACATTGAAGTAGCAATGGTTGAACCATCAGCCGTTGTTGCACATGGGTTTTATAAATCGAATATACAACCT
GGTATGACTGTTGCAGTAATGGGGTGTGGTAGCATAGGATTGTTAGCTATTCAATGGGCACGAATATTTGGTGCTGCACA
TATCATCGCTATAGATATAGATGCGCATAAACTAGATATTGCAAAATCATTGGGCGCACATCAAACAATCAATTCAAAAG
AAGAAAACCTTGAGAAATTCATCGAAAATCATTACGCCAATCAAATCGATTTAGCTATAGAATCATCAGGTGCTAAAGTT
ACGATTGGTCAAATATTGACGCTACCGAAAAAAGGTGGCGAGGTGGTATTACTCGGAATACCATATGATGATATTGAGAT
TGATCGAGTTCATTTTGAAAAAATTCTGCGTAACGAGTTGACAATATGTGGCTCTTGGAACTGTTTGTCCAGTAATTTTC
CGGGGAAAGAGTGGACGGCAACCTTATATTATATGAAGACGAAAGATATTAATGTAAAGCCTATTATTTCTCATTTTTTA
CCGTTAGAAAAAGGCCCGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTA
TTAG

Upstream 100 bases:

>100_bases
TACTCGTAAGTAATTCCATCGTCATTGTAGGTTATTTTGCGACAGGTATTTTTAAAAAGAAAAAAGTTGTGAATTAAATC
AGAGTTGAGGTGATTTACAA

Downstream 100 bases:

>100_bases
CATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATAAAAGTGCGAATACCGTCTGGTCTACAGATACTATCG
AAATAATTCATCTTCGAATA

Product: zinc-binding sorbitol dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MKALKLYGVEDLRYEDNDKPVIESANDVIVKVRATGICGSDTSRYKKMGPYIKGMPFGHEFSGVVDAIGSDVTHVNVGGK
VTGCPAIPCYQCEYCLKGEYARCKKLFVIGSYEPGSFAEYVKLPAQNVLKVPDNVDYIEVAMVEPSAVVAHGFYKSNIQP
GMTVAVMGCGSIGLLAIQWARIFGAAHIIAIDIDAHKLDIAKSLGAHQTINSKEENLEKFIENHYANQIDLAIESSGAKV
TIGQILTLPKKGGEVVLLGIPYDDIEIDRVHFEKILRNELTICGSWNCLSSNFPGKEWTATLYYMKTKDINVKPIISHFL
PLEKGPETFDKLVNKKERFDKVMFTIY

Sequences:

>Translated_347_residues
MKALKLYGVEDLRYEDNDKPVIESANDVIVKVRATGICGSDTSRYKKMGPYIKGMPFGHEFSGVVDAIGSDVTHVNVGGK
VTGCPAIPCYQCEYCLKGEYARCKKLFVIGSYEPGSFAEYVKLPAQNVLKVPDNVDYIEVAMVEPSAVVAHGFYKSNIQP
GMTVAVMGCGSIGLLAIQWARIFGAAHIIAIDIDAHKLDIAKSLGAHQTINSKEENLEKFIENHYANQIDLAIESSGAKV
TIGQILTLPKKGGEVVLLGIPYDDIEIDRVHFEKILRNELTICGSWNCLSSNFPGKEWTATLYYMKTKDINVKPIISHFL
PLEKGPETFDKLVNKKERFDKVMFTIY
>Mature_347_residues
MKALKLYGVEDLRYEDNDKPVIESANDVIVKVRATGICGSDTSRYKKMGPYIKGMPFGHEFSGVVDAIGSDVTHVNVGGK
VTGCPAIPCYQCEYCLKGEYARCKKLFVIGSYEPGSFAEYVKLPAQNVLKVPDNVDYIEVAMVEPSAVVAHGFYKSNIQP
GMTVAVMGCGSIGLLAIQWARIFGAAHIIAIDIDAHKLDIAKSLGAHQTINSKEENLEKFIENHYANQIDLAIESSGAKV
TIGQILTLPKKGGEVVLLGIPYDDIEIDRVHFEKILRNELTICGSWNCLSSNFPGKEWTATLYYMKTKDINVKPIISHFL
PLEKGPETFDKLVNKKERFDKVMFTIY

Specific function: Converts galactitol 1-phosphate to tagatose 6-phosphate [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=351, Percent_Identity=30.7692307692308, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI71565152, Length=355, Percent_Identity=25.0704225352113, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI4501929, Length=351, Percent_Identity=26.7806267806268, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI4501933, Length=344, Percent_Identity=26.453488372093, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI34577061, Length=344, Percent_Identity=25.8720930232558, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI71743840, Length=348, Percent_Identity=23.5632183908046, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI262073058, Length=348, Percent_Identity=23.5632183908046, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI4501939, Length=342, Percent_Identity=24.8538011695906, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI156523966, Length=344, Percent_Identity=24.7093023255814, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI71565154, Length=216, Percent_Identity=26.8518518518519, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1788407, Length=310, Percent_Identity=36.4516129032258, Blast_Score=210, Evalue=9e-56,
Organism=Escherichia coli, GI1788073, Length=335, Percent_Identity=30.7462686567164, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1790045, Length=341, Percent_Identity=30.791788856305, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1788075, Length=356, Percent_Identity=27.5280898876405, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI226510992, Length=346, Percent_Identity=26.0115606936416, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI1787863, Length=348, Percent_Identity=27.8735632183908, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1786825, Length=398, Percent_Identity=26.8844221105528, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790718, Length=307, Percent_Identity=26.7100977198697, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI87082125, Length=344, Percent_Identity=26.1627906976744, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87081918, Length=246, Percent_Identity=28.8617886178862, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1786552, Length=337, Percent_Identity=26.4094955489614, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17562876, Length=271, Percent_Identity=36.90036900369, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17562878, Length=245, Percent_Identity=32.6530612244898, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI71988145, Length=267, Percent_Identity=30.3370786516854, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25146526, Length=300, Percent_Identity=25.6666666666667, Blast_Score=78, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17562584, Length=268, Percent_Identity=28.7313432835821, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17565904, Length=267, Percent_Identity=25.4681647940075, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71997431, Length=302, Percent_Identity=25.4966887417219, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17562582, Length=278, Percent_Identity=28.7769784172662, Blast_Score=68, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6322619, Length=342, Percent_Identity=31.8713450292398, Blast_Score=139, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6319955, Length=342, Percent_Identity=31.8713450292398, Blast_Score=139, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6323099, Length=322, Percent_Identity=29.5031055900621, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6319258, Length=386, Percent_Identity=25.3886010362694, Blast_Score=100, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6319621, Length=340, Percent_Identity=28.2352941176471, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320033, Length=348, Percent_Identity=25, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6319257, Length=384, Percent_Identity=25.2604166666667, Blast_Score=86, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6323961, Length=257, Percent_Identity=27.6264591439689, Blast_Score=83, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6323729, Length=254, Percent_Identity=27.9527559055118, Blast_Score=80, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324486, Length=282, Percent_Identity=26.241134751773, Blast_Score=80, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6319949, Length=250, Percent_Identity=29.2, Blast_Score=73, Evalue=8e-14,
Organism=Drosophila melanogaster, GI17137530, Length=335, Percent_Identity=29.2537313432836, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI17737897, Length=338, Percent_Identity=30.1775147928994, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI17737895, Length=350, Percent_Identity=25.4285714285714, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI221457811, Length=307, Percent_Identity=22.4755700325733, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI45550770, Length=307, Percent_Identity=22.4755700325733, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI45551930, Length=307, Percent_Identity=22.4755700325733, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.251 [H]

Molecular weight: Translated: 38410; Mature: 38410

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALKLYGVEDLRYEDNDKPVIESANDVIVKVRATGICGSDTSRYKKMGPYIKGMPFGHE
CCCEEEECCCCCCCCCCCCCCEECCCEEEEEEEEEEECCCCHHHHHHHCCCCCCCCCCCH
FSGVVDAIGSDVTHVNVGGKVTGCPAIPCYQCEYCLKGEYARCKKLFVIGSYEPGSFAEY
HHHHHHHHCCCEEEEECCCEECCCCCCCCCCCCHHHCCCCHHEEEEEEEECCCCCCHHHH
VKLPAQNVLKVPDNVDYIEVAMVEPSAVVAHGFYKSNIQPGMTVAVMGCGSIGLLAIQWA
HHCCCHHEEECCCCCCEEEEEEECCCCEEEECEECCCCCCCCEEEEEECCCHHHHHHHHH
RIFGAAHIIAIDIDAHKLDIAKSLGAHQTINSKEENLEKFIENHYANQIDLAIESSGAKV
HHHCCEEEEEEEECCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCEE
TIGQILTLPKKGGEVVLLGIPYDDIEIDRVHFEKILRNELTICGSWNCLSSNFPGKEWTA
EEEEEEECCCCCCEEEEEECCCCCCEEHHHHHHHHHHHCEEEEECCCCCCCCCCCCCEEE
TLYYMKTKDINVKPIISHFLPLEKGPETFDKLVNKKERFDKVMFTIY
EEEEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEEEEEC
>Mature Secondary Structure
MKALKLYGVEDLRYEDNDKPVIESANDVIVKVRATGICGSDTSRYKKMGPYIKGMPFGHE
CCCEEEECCCCCCCCCCCCCCEECCCEEEEEEEEEEECCCCHHHHHHHCCCCCCCCCCCH
FSGVVDAIGSDVTHVNVGGKVTGCPAIPCYQCEYCLKGEYARCKKLFVIGSYEPGSFAEY
HHHHHHHHCCCEEEEECCCEECCCCCCCCCCCCHHHCCCCHHEEEEEEEECCCCCCHHHH
VKLPAQNVLKVPDNVDYIEVAMVEPSAVVAHGFYKSNIQPGMTVAVMGCGSIGLLAIQWA
HHCCCHHEEECCCCCCEEEEEEECCCCEEEECEECCCCCCCCEEEEEECCCHHHHHHHHH
RIFGAAHIIAIDIDAHKLDIAKSLGAHQTINSKEENLEKFIENHYANQIDLAIESSGAKV
HHHCCEEEEEEEECCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCEE
TIGQILTLPKKGGEVVLLGIPYDDIEIDRVHFEKILRNELTICGSWNCLSSNFPGKEWTA
EEEEEEECCCCCCEEEEEECCCCCCEEHHHHHHHHHHHCEEEEECCCCCCCCCCCCCEEE
TLYYMKTKDINVKPIISHFLPLEKGPETFDKLVNKKERFDKVMFTIY
EEEEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]