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The map label for this gene is capO

Identifier: 82749876

GI number: 82749876

Start: 126619

End: 127881

Strand: Direct

Name: capO

Synonym: SAB0104

Alternate gene names: 82749876

Gene position: 126619-127881 (Clockwise)

Preceding gene: 82749875

Following gene: 82749877

Centisome position: 4.62

GC content: 34.05

Gene sequence:

>1263_bases
ATGAAGTTAACAGTAGTTGGCTTAGGTTATATTGGTTTACCAACATCAATTATGTTTGCAAAGCATGGCGTCGATGTGCT
TGGTATTGATATTAATCAGCAAACGATTGATAAGTTACAAAGTGGTCAAATTAGTATTGAAGAACCTGGATTACAAGAGG
TTTATGAAGAGGTACTGTCATCGGGAAAATTGAAGGTATCTACAACGCCAGAAGCATCTGATGTTTTTATCATTGCTGTT
CCGACGCCGAATAATGATGATCAGTACCGGTCATGTGACATTTCGCTAGTTATGCGTGCATTAGATAGTATTTTACCATT
TTTAGAAAAAGGAAATACTATTATTGTAGAGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTG
AAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTACATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTAGAA
GAATTAGTTCATAACAATCGCATCATTGGCGGTGTGACTAAAGCTTGTATTGAAGCGGGTAAACGTGTCTATCGCACATT
TGTTCAGGGAAAAATGATTGAAACAGATGCACGTACTGCTGAAATGAGTAAGCTAATGGAAAACACATATAGAGACGTGA
ATATTGCTTTAGCTAATGAATTAACAAAAATTTGCAGTAACTTAAATATTAATGTATTAGATGTGATTGAAATGGCAAAC
AAACATCCGCGTGTTAATATCCATCAACCTGGTCCAGGTGTAGGCGGTCATTGTTTAGCTGTTGATCCGTACTTTATTAT
TGCTAAAGACCCTGAAAATGCAAAGTTAATTCAAACTGGACGTGAAATTAATAATTCAATGCCGGCCTATGTTGTTGATA
CAACGAAGCAAATCATCAAAGCGTTGAGCGGGAATAAAGTCACAGTATTTGGTTTAACTTATAAAGGCGATGTTGATGAT
ATAAGAGAATCACCAGCATTTGATATTTATGAGCTATTAAATCAAGAACCAGACATAGAAGTATGTGCTTATGATCCACA
TGTTGAATTAGATTTTGTGGAACATGATATGTCACATGCTGTCAAAGACGCATCGCTAGTATTGATTTTAAGTGACCACT
CAGAATTTAAAAATTTATCGGACAGTCATTTTGATAAAATGAAGCATAAAGTGATTTTTGATACAAAAAATGTTGTGAAA
TCATCATTTGAAGATGTATCGTATTATAATTATGGCAATATATTTAATTTTATCGACAAATAA

Upstream 100 bases:

>100_bases
TTATTGCGGACATCATGGATGAAACGACAACCAAAGATAAGGCATAAGTCATCTATTAAATAAAATCAACATACAAATTG
TTTTATTTGGAGGTTATAGT

Downstream 100 bases:

>100_bases
AATGTGTCAAATTAGGGCATACATGATTAAGGAAAGATAAGCTGTCATGTGTTTGAACTTCAGAGAGGATAATGTTATGA
AAAAAATTATGGTTATTTTC

Product: capsular polysaccharide synthesis enzyme CapO

Products: UDP-N-acetyl-D-mannosaminuronic-acid; NADH; Proton [C]

Alternate protein names: UDP-ManNAc 6-dehydrogenase [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MKLTVVGLGYIGLPTSIMFAKHGVDVLGIDINQQTIDKLQSGQISIEEPGLQEVYEEVLSSGKLKVSTTPEASDVFIIAV
PTPNNDDQYRSCDISLVMRALDSILPFLEKGNTIIVESTIAPKTMDDFVKPVIENLGFTIGEDIYLVHCPERVLPGKILE
ELVHNNRIIGGVTKACIEAGKRVYRTFVQGKMIETDARTAEMSKLMENTYRDVNIALANELTKICSNLNINVLDVIEMAN
KHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKLIQTGREINNSMPAYVVDTTKQIIKALSGNKVTVFGLTYKGDVDD
IRESPAFDIYELLNQEPDIEVCAYDPHVELDFVEHDMSHAVKDASLVLILSDHSEFKNLSDSHFDKMKHKVIFDTKNVVK
SSFEDVSYYNYGNIFNFIDK

Sequences:

>Translated_420_residues
MKLTVVGLGYIGLPTSIMFAKHGVDVLGIDINQQTIDKLQSGQISIEEPGLQEVYEEVLSSGKLKVSTTPEASDVFIIAV
PTPNNDDQYRSCDISLVMRALDSILPFLEKGNTIIVESTIAPKTMDDFVKPVIENLGFTIGEDIYLVHCPERVLPGKILE
ELVHNNRIIGGVTKACIEAGKRVYRTFVQGKMIETDARTAEMSKLMENTYRDVNIALANELTKICSNLNINVLDVIEMAN
KHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKLIQTGREINNSMPAYVVDTTKQIIKALSGNKVTVFGLTYKGDVDD
IRESPAFDIYELLNQEPDIEVCAYDPHVELDFVEHDMSHAVKDASLVLILSDHSEFKNLSDSHFDKMKHKVIFDTKNVVK
SSFEDVSYYNYGNIFNFIDK
>Mature_420_residues
MKLTVVGLGYIGLPTSIMFAKHGVDVLGIDINQQTIDKLQSGQISIEEPGLQEVYEEVLSSGKLKVSTTPEASDVFIIAV
PTPNNDDQYRSCDISLVMRALDSILPFLEKGNTIIVESTIAPKTMDDFVKPVIENLGFTIGEDIYLVHCPERVLPGKILE
ELVHNNRIIGGVTKACIEAGKRVYRTFVQGKMIETDARTAEMSKLMENTYRDVNIALANELTKICSNLNINVLDVIEMAN
KHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKLIQTGREINNSMPAYVVDTTKQIIKALSGNKVTVFGLTYKGDVDD
IRESPAFDIYELLNQEPDIEVCAYDPHVELDFVEHDMSHAVKDASLVLILSDHSEFKNLSDSHFDKMKHKVIFDTKNVVK
SSFEDVSYYNYGNIFNFIDK

Specific function: Catalyzes the four-electron oxidation of UDP-N-acetyl-D- mannosamine (UDP-ManNAc), reducing NAD(+) and releasing UDP-N- acetylmannosaminuronic acid (UDP-ManNAcA) [H]

COG id: COG0677

COG function: function code M; UDP-N-acetyl-D-mannosaminuronate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-glucose/GDP-mannose dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4507813, Length=451, Percent_Identity=23.2815964523282, Blast_Score=90, Evalue=5e-18,
Organism=Escherichia coli, GI48994968, Length=420, Percent_Identity=41.6666666666667, Blast_Score=340, Evalue=1e-94,
Organism=Escherichia coli, GI1788340, Length=421, Percent_Identity=24.2280285035629, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17560350, Length=462, Percent_Identity=23.3766233766234, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI17136908, Length=449, Percent_Identity=22.71714922049, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR017476
- InterPro:   IPR014027
- InterPro:   IPR014026
- InterPro:   IPR014028
- InterPro:   IPR001732 [H]

Pfam domain/function: PF00984 UDPG_MGDP_dh; PF03720 UDPG_MGDP_dh_C; PF03721 UDPG_MGDP_dh_N [H]

EC number: 1.1.1.- [C]

Molecular weight: Translated: 46782; Mature: 46782

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTVVGLGYIGLPTSIMFAKHGVDVLGIDINQQTIDKLQSGQISIEEPGLQEVYEEVLS
CEEEEEEECCCCCCHHHHHHHCCCEEEEEECCHHHHHHHHCCCEEECCCCHHHHHHHHHC
SGKLKVSTTPEASDVFIIAVPTPNNDDQYRSCDISLVMRALDSILPFLEKGNTIIVESTI
CCCEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
APKTMDDFVKPVIENLGFTIGEDIYLVHCPERVLPGKILEELVHNNRIIGGVTKACIEAG
CCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCEEEHHHHHHHHHHH
KRVYRTFVQGKMIETDARTAEMSKLMENTYRDVNIALANELTKICSNLNINVLDVIEMAN
HHHHHHHHCCCEEECCHHHHHHHHHHHHHHCCEEEHHHHHHHHHHCCCCCCHHHHHHHHC
KHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKLIQTGREINNSMPAYVVDTTKQIIK
CCCEEEEECCCCCCCCEEEEECCEEEEEECCCCCHHHHCCHHHCCCCCEEEEHHHHHHHH
ALSGNKVTVFGLTYKGDVDDIRESPAFDIYELLNQEPDIEVCAYDPHVELDFVEHDMSHA
HHCCCEEEEEEEEECCCHHHHHCCCCHHHHHHHCCCCCEEEEEECCCEEEEHHHHHHHHH
VKDASLVLILSDHSEFKNLSDSHFDKMKHKVIFDTKNVVKSSFEDVSYYNYGNIFNFIDK
HCCCEEEEEEECCHHHHCCCHHHHHHHHHHEEECCHHHHHHHHHHCCEECCCCHHHHHCC
>Mature Secondary Structure
MKLTVVGLGYIGLPTSIMFAKHGVDVLGIDINQQTIDKLQSGQISIEEPGLQEVYEEVLS
CEEEEEEECCCCCCHHHHHHHCCCEEEEEECCHHHHHHHHCCCEEECCCCHHHHHHHHHC
SGKLKVSTTPEASDVFIIAVPTPNNDDQYRSCDISLVMRALDSILPFLEKGNTIIVESTI
CCCEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
APKTMDDFVKPVIENLGFTIGEDIYLVHCPERVLPGKILEELVHNNRIIGGVTKACIEAG
CCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCEEEHHHHHHHHHHH
KRVYRTFVQGKMIETDARTAEMSKLMENTYRDVNIALANELTKICSNLNINVLDVIEMAN
HHHHHHHHCCCEEECCHHHHHHHHHHHHHHCCEEEHHHHHHHHHHCCCCCCHHHHHHHHC
KHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKLIQTGREINNSMPAYVVDTTKQIIK
CCCEEEEECCCCCCCCEEEEECCEEEEEECCCCCHHHHCCHHHCCCCCEEEEHHHHHHHH
ALSGNKVTVFGLTYKGDVDDIRESPAFDIYELLNQEPDIEVCAYDPHVELDFVEHDMSHA
HHCCCEEEEEEEEECCCHHHHHCCCCHHHHHHHCCCCCEEEEEECCCEEEEHHHHHHHHH
VKDASLVLILSDHSEFKNLSDSHFDKMKHKVIFDTKNVVKSSFEDVSYYNYGNIFNFIDK
HCCCEEEEEEECCHHHHCCCHHHHHHHHHHEEECCHHHHHHHHHHCCEECCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-acetyl-D-mannosamine; NAD [C]

Specific reaction: UDP-N-acetyl-D-mannosamine + NAD = UDP-N-acetyl-D-mannosaminuronic-acid + NADH + Proton [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA