Click here to switch to the map view.

The map label for this gene is yrkE [H]

Identifier: 82749807

GI number: 82749807

Start: 40008

End: 41075

Strand: Direct

Name: yrkE [H]

Synonym: SAB0030

Alternate gene names: 82749807

Gene position: 40008-41075 (Clockwise)

Preceding gene: 82749803

Following gene: 82749808

Centisome position: 1.46

GC content: 31.93

Gene sequence:

>1068_bases
ATGAAACAATACGGAGAAAAGTTTATCGATGAATTTAGTAAAGCAGAATTGGAAAAACTAGCAAAGCAAGGGCAATTAAT
TGATGTTAGAACAGAAGAGGAGTATGCATTAGGACATATCAATGGTTCCATACTTCATCCTGTTGATGAGATTGAGTCAT
TCAATAAAGAAAAAAATAAAACCTATTATGTAATCTGTAGAAGTGGTAACAGAAGTGCTAATGCTAGTAAATATTTAGCT
AAACAAGGTTATAACGTTATAAATCTTGATGGTGGTTATAAAGTTTATGAAGAAGAAAACGATAGTTATGATACGCAAGA
AGAATATAAAAGTATAGAAATTAAAGCAGATCGTAAACAATTTAACTATCGTGGTCTTCAATGTCCAGGGCCAATTGTAA
AAATTAGTCAAGAAATGAAGAATATTGAAGTAGGTGACCAAATTGAAGTCAAAGTCACAGACCCTGGATTCCCTAGTGAT
ATTAAAAGTTGGGTGAAACAAACAAGGCATACTTTAGTTAAGCTTGATGAAAATAACAATGGAATTAATGCGATTATTCA
AAAAGAAAAAGCAAAAGATTTAGATGTAAACTATTCTGCTAAAGGTACTACAATTGTATTATTTAGTGGAGAATTAGACA
AAGCTGTAGCAGCATTGATTATTGCAAATGGCGCTAGAGCTGCTGGAAAAGATGTAACTATCTTCTTTACTTTTTGGGGG
CTTAATGCATTAAAAAAAGCGCAAACAGTTAATGTTAAAAAGCAAGGTATTGCAAAAATGTTTGATTTAATGTTGCCCAA
AAAGAATATACGAATGCCTCTTTCCAAAATGAATATGTTTGGTTTAGGAAATATGATGATGCGCTACGTAATGAGAAAGA
AAAATGTTGATTCATTACCAACACTTATCAATCAAGCTATTGAGCAAAATATCAAATTAATCGCTTGTACGATGAGTATG
GATGTCATGGGTATTCAGAAAGAAGAACTTAGAGATGAAGTTGAGTACGGTGGTGTAGGCACTTATATTGGTGCTACTGA
AAATGCGAATCATAATTTATTTATCTAA

Upstream 100 bases:

>100_bases
TAATAGATAAACACAAATGCGTCAAATACCCCTAGAGGTATTTGGCAAGTTCCATTCAACTGTTTATAATACCCCTACAG
GTATTTTTAGGGAGGTTATT

Downstream 100 bases:

>100_bases
TTAAATCTATTAATAAAAGGAGTTGTTATCATGTTTTTTAAACAGTTTTACGATAATCATTTATCTCAAGCATCATATTT
AGTGGGTTGTCAACGTACAG

Product: hypothetical protein

Products: H+; Sulfite; Thiocyanate [C]

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MKQYGEKFIDEFSKAELEKLAKQGQLIDVRTEEEYALGHINGSILHPVDEIESFNKEKNKTYYVICRSGNRSANASKYLA
KQGYNVINLDGGYKVYEEENDSYDTQEEYKSIEIKADRKQFNYRGLQCPGPIVKISQEMKNIEVGDQIEVKVTDPGFPSD
IKSWVKQTRHTLVKLDENNNGINAIIQKEKAKDLDVNYSAKGTTIVLFSGELDKAVAALIIANGARAAGKDVTIFFTFWG
LNALKKAQTVNVKKQGIAKMFDLMLPKKNIRMPLSKMNMFGLGNMMMRYVMRKKNVDSLPTLINQAIEQNIKLIACTMSM
DVMGIQKEELRDEVEYGGVGTYIGATENANHNLFI

Sequences:

>Translated_355_residues
MKQYGEKFIDEFSKAELEKLAKQGQLIDVRTEEEYALGHINGSILHPVDEIESFNKEKNKTYYVICRSGNRSANASKYLA
KQGYNVINLDGGYKVYEEENDSYDTQEEYKSIEIKADRKQFNYRGLQCPGPIVKISQEMKNIEVGDQIEVKVTDPGFPSD
IKSWVKQTRHTLVKLDENNNGINAIIQKEKAKDLDVNYSAKGTTIVLFSGELDKAVAALIIANGARAAGKDVTIFFTFWG
LNALKKAQTVNVKKQGIAKMFDLMLPKKNIRMPLSKMNMFGLGNMMMRYVMRKKNVDSLPTLINQAIEQNIKLIACTMSM
DVMGIQKEELRDEVEYGGVGTYIGATENANHNLFI
>Mature_355_residues
MKQYGEKFIDEFSKAELEKLAKQGQLIDVRTEEEYALGHINGSILHPVDEIESFNKEKNKTYYVICRSGNRSANASKYLA
KQGYNVINLDGGYKVYEEENDSYDTQEEYKSIEIKADRKQFNYRGLQCPGPIVKISQEMKNIEVGDQIEVKVTDPGFPSD
IKSWVKQTRHTLVKLDENNNGINAIIQKEKAKDLDVNYSAKGTTIVLFSGELDKAVAALIIANGARAAGKDVTIFFTFWG
LNALKKAQTVNVKKQGIAKMFDLMLPKKNIRMPLSKMNMFGLGNMMMRYVMRKKNVDSLPTLINQAIEQNIKLIACTMSM
DVMGIQKEELRDEVEYGGVGTYIGATENANHNLFI

Specific function: Catalyzes, Although With Low Efficiency, The Sulfur Transfer Reaction From Thiosulfate To Cyanide. The Relatively Low Affinity Of Glpe For Both Thiosulfate And Cyanide Suggests That These Compounds Are Not The Physiological Substrates. Thioredoxin 1 Or Re

COG id: COG2210

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003787 [H]

Pfam domain/function: PF02635 DrsE [H]

EC number: 2.8.1.1 [C]

Molecular weight: Translated: 40102; Mature: 40102

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYGEKFIDEFSKAELEKLAKQGQLIDVRTEEEYALGHINGSILHPVDEIESFNKEKNK
CCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCEECCHHHHHHCCCCCCC
TYYVICRSGNRSANASKYLAKQGYNVINLDGGYKVYEEENDSYDTQEEYKSIEIKADRKQ
EEEEEECCCCCCCCHHHHHHHCCCCEEEECCCEEEEECCCCCCCCHHHHHEEEEEECCHH
FNYRGLQCPGPIVKISQEMKNIEVGDQIEVKVTDPGFPSDIKSWVKQTRHTLVKLDENNN
CCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHCEEEEEECCCC
GINAIIQKEKAKDLDVNYSAKGTTIVLFSGELDKAVAALIIANGARAAGKDVTIFFTFWG
CEEEEEECCCCCCCCCEECCCCCEEEEECCCHHHHHHHHHEECCCCCCCCCEEEEEEECC
LNALKKAQTVNVKKQGIAKMFDLMLPKKNIRMPLSKMNMFGLGNMMMRYVMRKKNVDSLP
CHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHH
TLINQAIEQNIKLIACTMSMDVMGIQKEELRDEVEYGGVGTYIGATENANHNLFI
HHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCEEC
>Mature Secondary Structure
MKQYGEKFIDEFSKAELEKLAKQGQLIDVRTEEEYALGHINGSILHPVDEIESFNKEKNK
CCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCEECCHHHHHHCCCCCCC
TYYVICRSGNRSANASKYLAKQGYNVINLDGGYKVYEEENDSYDTQEEYKSIEIKADRKQ
EEEEEECCCCCCCCHHHHHHHCCCCEEEECCCEEEEECCCCCCCCHHHHHEEEEEECCHH
FNYRGLQCPGPIVKISQEMKNIEVGDQIEVKVTDPGFPSDIKSWVKQTRHTLVKLDENNN
CCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHCEEEEEECCCC
GINAIIQKEKAKDLDVNYSAKGTTIVLFSGELDKAVAALIIANGARAAGKDVTIFFTFWG
CEEEEEECCCCCCCCCEECCCCCEEEEECCCHHHHHHHHHEECCCCCCCCCEEEEEEECC
LNALKKAQTVNVKKQGIAKMFDLMLPKKNIRMPLSKMNMFGLGNMMMRYVMRKKNVDSLP
CHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHH
TLINQAIEQNIKLIACTMSMDVMGIQKEELRDEVEYGGVGTYIGATENANHNLFI
HHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cyanide; Thiosulfate [C]

Specific reaction: Cyanide + Thiosulfate --> H+ + Sulfite + Thiocyanate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]