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The map label for this gene is ruvC [H]

Identifier: 82703834

GI number: 82703834

Start: 3121150

End: 3121551

Strand: Reverse

Name: ruvC [H]

Synonym: Nmul_A2721

Alternate gene names: 82703834

Gene position: 3121551-3121150 (Counterclockwise)

Preceding gene: 82703835

Following gene: 82703833

Centisome position: 98.03

GC content: 58.21

Gene sequence:

>402_bases
TTGCCGTTGCGCCTCAAATCCATCATGGATCACCTCAACGAAGTCATCGCGGAACACAAGCCTGACCATGTCGCGATCGA
GCAGGTGTTTGTCAACGTCAATCCCAAATCCACCTTGATGCTCGGGCAGGCGCGGGGAGCGGCGATATGCACGGCAGTGC
TGAATAACCTGTCCGTTGCAGAGTATACGGCGCTGCAGATCAAGCAGGCGGTGGTCGGTGAAGGCCACGCGCGGAAAGAA
CAGGTACAGGAAATGGTAAGACGGTTATTGCGCCTCTCCGGTCAGCCTGCATCGGATGCCGCCGATGCCCTTGCCTGTGC
TATTTGCCATGCCCATGGAGGCTTGGGACTGGGTGCCCTGTCGACCGCCGGTTACCGGGTAAAACAGGGGCGTCTCATAT
GA

Upstream 100 bases:

>100_bases
AGACCCAGGTCTGCGGCTAACCGGTTTTGGAATCATCGACAAAACCGGCGGCAAACTGGTCTACGTCAGCAGTGGCCGCA
TCAGGACAGTAGAGGGTGAA

Downstream 100 bases:

>100_bases
TCGGGAGAATAAGCGGACTCCTGCTGGAAAAATTTCCCCCGCTGGTGCTGGTTGATGTCCAGGGTGTGGGCTATGAGATC
GACGTACCCATGAGCACGTT

Product: crossover junction endodeoxyribonuclease RuvC

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC [H]

Number of amino acids: Translated: 133; Mature: 132

Protein sequence:

>133_residues
MPLRLKSIMDHLNEVIAEHKPDHVAIEQVFVNVNPKSTLMLGQARGAAICTAVLNNLSVAEYTALQIKQAVVGEGHARKE
QVQEMVRRLLRLSGQPASDAADALACAICHAHGGLGLGALSTAGYRVKQGRLI

Sequences:

>Translated_133_residues
MPLRLKSIMDHLNEVIAEHKPDHVAIEQVFVNVNPKSTLMLGQARGAAICTAVLNNLSVAEYTALQIKQAVVGEGHARKE
QVQEMVRRLLRLSGQPASDAADALACAICHAHGGLGLGALSTAGYRVKQGRLI
>Mature_132_residues
PLRLKSIMDHLNEVIAEHKPDHVAIEQVFVNVNPKSTLMLGQARGAAICTAVLNNLSVAEYTALQIKQAVVGEGHARKEQ
VQEMVRRLLRLSGQPASDAADALACAICHAHGGLGLGALSTAGYRVKQGRLI

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family [H]

Homologues:

Organism=Escherichia coli, GI1788170, Length=132, Percent_Identity=50.7575757575758, Blast_Score=101, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176 [H]

Pfam domain/function: PF02075 RuvC [H]

EC number: =3.1.22.4 [H]

Molecular weight: Translated: 14213; Mature: 14082

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLRLKSIMDHLNEVIAEHKPDHVAIEQVFVNVNPKSTLMLGQARGAAICTAVLNNLSVA
CCCHHHHHHHHHHHHHHHCCCCCEEEEHHEEECCCCCEEEEECCCCHHHHHHHHHCCCHH
EYTALQIKQAVVGEGHARKEQVQEMVRRLLRLSGQPASDAADALACAICHAHGGLGLGAL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHH
STAGYRVKQGRLI
HHCCEECCCCCCC
>Mature Secondary Structure 
PLRLKSIMDHLNEVIAEHKPDHVAIEQVFVNVNPKSTLMLGQARGAAICTAVLNNLSVA
CCHHHHHHHHHHHHHHHCCCCCEEEEHHEEECCCCCEEEEECCCCHHHHHHHHHCCCHH
EYTALQIKQAVVGEGHARKEQVQEMVRRLLRLSGQPASDAADALACAICHAHGGLGLGAL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHH
STAGYRVKQGRLI
HHCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA