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The map label for this gene is nth [H]

Identifier: 82703817

GI number: 82703817

Start: 3107117

End: 3107764

Strand: Reverse

Name: nth [H]

Synonym: Nmul_A2704

Alternate gene names: 82703817

Gene position: 3107764-3107117 (Counterclockwise)

Preceding gene: 82703818

Following gene: 82703816

Centisome position: 97.6

GC content: 50.77

Gene sequence:

>648_bases
ATGAATTCAAGCATACGCCGTGAGATTTTTACCCGCTTCAGGGCCGCCAATCCTCATCCTACCACTGAGCTGGAGTACAA
CTCCCCCTTTGAACTGCTGGTAGCAGTGGCACTCTCCGCCCAAGCGACCGACAAAAGCGTGAACCTTGCCACGCGGAAGC
TCTTTCCCAAGGCCAATACACCCGAGGCAATACTTGCAATGGGGGAAGAAGCATTGAGGGAATACATCAAGAGCATCGGC
TTGTACAAAACAAAGGCAAGGAACATCCTGGCAACATGCCGCATACTCATTGATCAGTATGGCGGAAAAGTACCTGAAAC
GCGGGAGCAGCTGGAAAAACTGCCCGGCGTGGGGCGCAAGACTGCAAATGTGTTATTGAATACTGCCTTTGGTCAACCCA
CTATTGCGGTGGATACACATATTTTCCGCGTGGCCAATCGTACGGGAATTGCAAGGGGAAAGAACGTGCTGGAAGTGGAG
TCCAAGCTCCTCAAGTGCGTCCCCAAGGAATTCCGCCAGGATGCCCATCACTGGTTGATTTTGCATGGGCGCTATATCTG
CGTGGCAAGAAAACCAAAATGTGCGATCTGCCTGATAAACGACCTGTGTGAATACGAGTCAAAAAACTTTGAGGATACTC
CTCGTTAA

Upstream 100 bases:

>100_bases
GCTGGAGCGCGCAAGGGTTGCCAAGGCACAGCCATCGCCTGGAATATCAAAAAAGGAGGACTGGAGTGACAGAACGTCAG
ACATTTCTTCCCGCTGATTC

Downstream 100 bases:

>100_bases
AAAACTTGATGTTCAATCCTTCCAGAGACCAGGCGCGTCAATTTTTTTTCGAGACCTGGCGTAAATACAAGCAACGGGAA
ATGCTCAGCGCGATGGAGGA

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MNSSIRREIFTRFRAANPHPTTELEYNSPFELLVAVALSAQATDKSVNLATRKLFPKANTPEAILAMGEEALREYIKSIG
LYKTKARNILATCRILIDQYGGKVPETREQLEKLPGVGRKTANVLLNTAFGQPTIAVDTHIFRVANRTGIARGKNVLEVE
SKLLKCVPKEFRQDAHHWLILHGRYICVARKPKCAICLINDLCEYESKNFEDTPR

Sequences:

>Translated_215_residues
MNSSIRREIFTRFRAANPHPTTELEYNSPFELLVAVALSAQATDKSVNLATRKLFPKANTPEAILAMGEEALREYIKSIG
LYKTKARNILATCRILIDQYGGKVPETREQLEKLPGVGRKTANVLLNTAFGQPTIAVDTHIFRVANRTGIARGKNVLEVE
SKLLKCVPKEFRQDAHHWLILHGRYICVARKPKCAICLINDLCEYESKNFEDTPR
>Mature_215_residues
MNSSIRREIFTRFRAANPHPTTELEYNSPFELLVAVALSAQATDKSVNLATRKLFPKANTPEAILAMGEEALREYIKSIG
LYKTKARNILATCRILIDQYGGKVPETREQLEKLPGVGRKTANVLLNTAFGQPTIAVDTHIFRVANRTGIARGKNVLEVE
SKLLKCVPKEFRQDAHHWLILHGRYICVARKPKCAICLINDLCEYESKNFEDTPR

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=31.638418079096, Blast_Score=99, Evalue=2e-21,
Organism=Escherichia coli, GI1787920, Length=208, Percent_Identity=68.75, Blast_Score=302, Evalue=1e-83,
Organism=Escherichia coli, GI1789331, Length=133, Percent_Identity=30.8270676691729, Blast_Score=66, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17554540, Length=175, Percent_Identity=33.7142857142857, Blast_Score=101, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6319304, Length=174, Percent_Identity=31.0344827586207, Blast_Score=80, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324530, Length=190, Percent_Identity=26.8421052631579, Blast_Score=76, Evalue=4e-15,
Organism=Drosophila melanogaster, GI45550361, Length=177, Percent_Identity=32.7683615819209, Blast_Score=100, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24250; Mature: 24250

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSSIRREIFTRFRAANPHPTTELEYNSPFELLVAVALSAQATDKSVNLATRKLFPKANT
CCCHHHHHHHHHHHCCCCCCCCEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCC
PEAILAMGEEALREYIKSIGLYKTKARNILATCRILIDQYGGKVPETREQLEKLPGVGRK
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCH
TANVLLNTAFGQPTIAVDTHIFRVANRTGIARGKNVLEVESKLLKCVPKEFRQDAHHWLI
HHHHHHHHCCCCCEEEEHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEE
LHGRYICVARKPKCAICLINDLCEYESKNFEDTPR
EECCEEEEECCCCEEEHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNSSIRREIFTRFRAANPHPTTELEYNSPFELLVAVALSAQATDKSVNLATRKLFPKANT
CCCHHHHHHHHHHHCCCCCCCCEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCC
PEAILAMGEEALREYIKSIGLYKTKARNILATCRILIDQYGGKVPETREQLEKLPGVGRK
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCH
TANVLLNTAFGQPTIAVDTHIFRVANRTGIARGKNVLEVESKLLKCVPKEFRQDAHHWLI
HHHHHHHHCCCCCEEEEHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEE
LHGRYICVARKPKCAICLINDLCEYESKNFEDTPR
EECCEEEEECCCCEEEHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]