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The map label for this gene is dapF

Identifier: 82703657

GI number: 82703657

Start: 2911328

End: 2912161

Strand: Direct

Name: dapF

Synonym: Nmul_A2542

Alternate gene names: 82703657

Gene position: 2911328-2912161 (Clockwise)

Preceding gene: 82703653

Following gene: 82703658

Centisome position: 91.43

GC content: 54.92

Gene sequence:

>834_bases
ATGAAGCTCAAGTTCACCAAAATGCATGGCCTCGGCAACGATTTCATTGTCATTGATGCCGTGAATCAGCAGATTTCCCT
CTCACCCGAACAATTGCGGCGCCTTGCCGACCGCCACCTGGGAGTAGGCTGCGACCAGATTCTGCTGATCGAAAAGGCGG
AAGGGGATGCTGATTTCCGTTATCGCATTTTTAATGCCGACGGAGGAGAAGTGGAACAATGCGGCAATGGGGCGCGCTGC
TTCGTTCGCTACGTCCATGATCATGGTATGACGGACAAGCAGCAGGTAAGGATTGAAACGCTAAGCGGGGTGGTTATTCC
GGAGCTTGAAGCGGACGGGGAAGTAACGGTCAACATGGGTGTGCCGAAATTCGATCCCGTCGAAATTCCTTTTATCGCCG
AACAGCGAGCGCCTACTTACTCCCTCTCCCTGGATGACAGGCAGGTGGAAATCAGCAGTGTATCGATGGGCAATCCCCAC
GCGGTCCAGGTAGTATCGGATCTGGATAACGCGCCGGTTTTGACGGAGGGGCCAGTTATCGAAAAGCATTCTCGTTTTCC
TCAGCGAGTAAATGCCGGATATATGCAAGTCGTCGATCCTCATCATATCCGGCTGAGAGTCTACGAGCGGGGCGCGGGTG
AAACCCTGGCTTGCGGCACCGGTGCCTGTGCGGCGGCGGTTGCGGGAATTCAGCGAGGGCTGCTCGAATCGCCCGTCAGG
GTGAGCTTTTCCACTGGAGACCTGTTCATACGATGGGAAGGGGAAAATCAGCCGGTGTGGATGACCGGTCCTGCCGTCGC
CGTTTTTGATGGAGAAATCGAGTTGCAGTTTTAA

Upstream 100 bases:

>100_bases
AATTCTACGCTTATTAAGAAGACGGATAATACAATACGGCTTGACATGCCGATGTTTCCATACCTCGTGCTGTGATTCTT
ATTCTTGCAACGCTTGATGA

Downstream 100 bases:

>100_bases
AAACAGAAAAGATATGAAATCAAGTTTCGAAGAGATAGCAGAGTATCTGCGTGCGAATCCCGAGTTCTTTGAAGCCCATG
CGAGCATGCTGGCAGATATT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MKLKFTKMHGLGNDFIVIDAVNQQISLSPEQLRRLADRHLGVGCDQILLIEKAEGDADFRYRIFNADGGEVEQCGNGARC
FVRYVHDHGMTDKQQVRIETLSGVVIPELEADGEVTVNMGVPKFDPVEIPFIAEQRAPTYSLSLDDRQVEISSVSMGNPH
AVQVVSDLDNAPVLTEGPVIEKHSRFPQRVNAGYMQVVDPHHIRLRVYERGAGETLACGTGACAAAVAGIQRGLLESPVR
VSFSTGDLFIRWEGENQPVWMTGPAVAVFDGEIELQF

Sequences:

>Translated_277_residues
MKLKFTKMHGLGNDFIVIDAVNQQISLSPEQLRRLADRHLGVGCDQILLIEKAEGDADFRYRIFNADGGEVEQCGNGARC
FVRYVHDHGMTDKQQVRIETLSGVVIPELEADGEVTVNMGVPKFDPVEIPFIAEQRAPTYSLSLDDRQVEISSVSMGNPH
AVQVVSDLDNAPVLTEGPVIEKHSRFPQRVNAGYMQVVDPHHIRLRVYERGAGETLACGTGACAAAVAGIQRGLLESPVR
VSFSTGDLFIRWEGENQPVWMTGPAVAVFDGEIELQF
>Mature_277_residues
MKLKFTKMHGLGNDFIVIDAVNQQISLSPEQLRRLADRHLGVGCDQILLIEKAEGDADFRYRIFNADGGEVEQCGNGARC
FVRYVHDHGMTDKQQVRIETLSGVVIPELEADGEVTVNMGVPKFDPVEIPFIAEQRAPTYSLSLDDRQVEISSVSMGNPH
AVQVVSDLDNAPVLTEGPVIEKHSRFPQRVNAGYMQVVDPHHIRLRVYERGAGETLACGTGACAAAVAGIQRGLLESPVR
VSFSTGDLFIRWEGENQPVWMTGPAVAVFDGEIELQF

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=274, Percent_Identity=55.8394160583942, Blast_Score=313, Evalue=9e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_NITMU (Q2Y5Z0)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_413223.1
- HSSP:   P44859
- ProteinModelPortal:   Q2Y5Z0
- SMR:   Q2Y5Z0
- STRING:   Q2Y5Z0
- GeneID:   3784047
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A2542
- eggNOG:   COG0253
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- PhylomeDB:   Q2Y5Z0
- BioCyc:   NMUL323848:NMUL_A2542-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 30471; Mature: 30471

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 74-74 ACT_SITE 218-218

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKFTKMHGLGNDFIVIDAVNQQISLSPEQLRRLADRHLGVGCDQILLIEKAEGDADFR
CCEEEEECCCCCCCEEEEEECCCCEECCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEE
YRIFNADGGEVEQCGNGARCFVRYVHDHGMTDKQQVRIETLSGVVIPELEADGEVTVNMG
EEEEECCCCCHHHCCCCCEEEEEEEHHCCCCCHHEEEEEEECCEEEEEECCCCEEEEEEC
VPKFDPVEIPFIAEQRAPTYSLSLDDRQVEISSVSMGNPHAVQVVSDLDNAPVLTEGPVI
CCCCCCEECCEEECCCCCEEEEECCCCEEEEEEEECCCCEEEEEEECCCCCCEEECCCCC
EKHSRFPQRVNAGYMQVVDPHHIRLRVYERGAGETLACGTGACAAAVAGIQRGLLESPVR
CHHHCCCHHCCCCEEEEECCCEEEEEEEECCCCCEEECCCCHHHHHHHHHHHHHHCCCEE
VSFSTGDLFIRWEGENQPVWMTGPAVAVFDGEIELQF
EEEECCCEEEEEECCCCCEEEECCEEEEEECEEEEEC
>Mature Secondary Structure
MKLKFTKMHGLGNDFIVIDAVNQQISLSPEQLRRLADRHLGVGCDQILLIEKAEGDADFR
CCEEEEECCCCCCCEEEEEECCCCEECCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEE
YRIFNADGGEVEQCGNGARCFVRYVHDHGMTDKQQVRIETLSGVVIPELEADGEVTVNMG
EEEEECCCCCHHHCCCCCEEEEEEEHHCCCCCHHEEEEEEECCEEEEEECCCCEEEEEEC
VPKFDPVEIPFIAEQRAPTYSLSLDDRQVEISSVSMGNPHAVQVVSDLDNAPVLTEGPVI
CCCCCCEECCEEECCCCCEEEEECCCCEEEEEEEECCCCEEEEEEECCCCCCEEECCCCC
EKHSRFPQRVNAGYMQVVDPHHIRLRVYERGAGETLACGTGACAAAVAGIQRGLLESPVR
CHHHCCCHHCCCCEEEEECCCEEEEEEEECCCCCEEECCCCHHHHHHHHHHHHHHCCCEE
VSFSTGDLFIRWEGENQPVWMTGPAVAVFDGEIELQF
EEEECCCEEEEEECCCCCEEEECCEEEEEECEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA