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The map label for this gene is murB

Identifier: 82703607

GI number: 82703607

Start: 2846623

End: 2847666

Strand: Reverse

Name: murB

Synonym: Nmul_A2492

Alternate gene names: 82703607

Gene position: 2847666-2846623 (Counterclockwise)

Preceding gene: 82703608

Following gene: 82703606

Centisome position: 89.43

GC content: 58.33

Gene sequence:

>1044_bases
ATGGATATGTCGGAAATCAACTTCTCTCTTGATTATGCCGGCGTTCGCGGCGAACTGCGCCATGATCAGCCGATGAAGAA
CTATACTTCCTGGCGTGCGGGCGGGAGTGCAGAGCGCATCTATCTGCCGGGGGATTTGCCTGATCTCGCGGCGTTTCTGC
GCGGCCTGCCCTGGAATGAACCGGTATATGTGATGGGACTCGGCAGCAATCTTCTGGTACGCGATGGCGGGGTGCGGGGC
AGCGTCGTTGTACTGCACGCACGCCTGAATGGTTTACAGCTGGAATCCGACATGGGCCAGATGCTGATCTATGCCGGTGC
AGGTGTAGCATGTGCCAAAGTTGCCCGGTTTGCTGCGCTGCAGGGACTGGGGGGCGCGGAATTCCTGGCAGGTATTCCCG
GGACCGTGGGCGGCGCATTGGCAATGAATGCCGGATGCTATGGAACCGAAACCTGGGACATCGTCTCGAGTGTGCAAACC
ATAGACCGCTTGGGGATATTGCGCCGACGGCCGCCTGGGAATTACGAAATTGGCTATCGGCACGTGGCATTGAAGGCAGA
GAAGAGCAGTGGGAGCCAAAAGATGGGCGCCAGAGAAAATGCCCCTGACGATTCTCTGACGGACGAATGGTTTTCGGGCG
CATGGTTCGCGCTGCCGCGGGACCATGCAGCGGCAGTTCGGCAGAAGATCAAGGAGTTGCTTGCCCGCCGTATCCATACT
CAGCCTTTAAACCTGCCGAATGCGGGGTCCGTGTTCCGCAATCCGGAGAATGACAAAGCGGCGCGACTCATCGAGTCGTG
CGGACTTAAGGAGTTTCGTATCGGCGGCGCCATGGTTTCGCCGCGCCACGCAAATTTCATTGTGAACACCGGGGGTGCTA
CCGCTTCCGACATAGAAGCGGTGATTGCTGCGGTCCGTGAAACCGTAAAAAAGCAGACCGGCGTCGAACTGAAACAGGAG
GTGCGCATTATCGGGACCCCCGCCAGACCCGAGTTGCATGTCTCCCGGACTGCTTACAGAAAGGATGGTGGATATGGCGG
GTAG

Upstream 100 bases:

>100_bases
GGCCCCCACCCTTTCCCGGGATTCGCAGGTTGAAGCGCGTCTGCTCGTCAGCCCGAAAGAAAACAGGGAACCCGCAGTGC
TTTTGTTACAGGGTTCCTGA

Downstream 100 bases:

>100_bases
TCATGACTTCGGGAAGGTCGCGGTTCTCCTCGGAGGACGATCAGCGGAGCGCGAGATTTCCCTTGAAAGCGGTAAAGCCG
TACTCGATGCTCTGCGCAGC

Product: UDP-N-acetylenolpyruvoylglucosamine reductase

Products: NA

Alternate protein names: UDP-N-acetylmuramate dehydrogenase

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MDMSEINFSLDYAGVRGELRHDQPMKNYTSWRAGGSAERIYLPGDLPDLAAFLRGLPWNEPVYVMGLGSNLLVRDGGVRG
SVVVLHARLNGLQLESDMGQMLIYAGAGVACAKVARFAALQGLGGAEFLAGIPGTVGGALAMNAGCYGTETWDIVSSVQT
IDRLGILRRRPPGNYEIGYRHVALKAEKSSGSQKMGARENAPDDSLTDEWFSGAWFALPRDHAAAVRQKIKELLARRIHT
QPLNLPNAGSVFRNPENDKAARLIESCGLKEFRIGGAMVSPRHANFIVNTGGATASDIEAVIAAVRETVKKQTGVELKQE
VRIIGTPARPELHVSRTAYRKDGGYGG

Sequences:

>Translated_347_residues
MDMSEINFSLDYAGVRGELRHDQPMKNYTSWRAGGSAERIYLPGDLPDLAAFLRGLPWNEPVYVMGLGSNLLVRDGGVRG
SVVVLHARLNGLQLESDMGQMLIYAGAGVACAKVARFAALQGLGGAEFLAGIPGTVGGALAMNAGCYGTETWDIVSSVQT
IDRLGILRRRPPGNYEIGYRHVALKAEKSSGSQKMGARENAPDDSLTDEWFSGAWFALPRDHAAAVRQKIKELLARRIHT
QPLNLPNAGSVFRNPENDKAARLIESCGLKEFRIGGAMVSPRHANFIVNTGGATASDIEAVIAAVRETVKKQTGVELKQE
VRIIGTPARPELHVSRTAYRKDGGYGG
>Mature_347_residues
MDMSEINFSLDYAGVRGELRHDQPMKNYTSWRAGGSAERIYLPGDLPDLAAFLRGLPWNEPVYVMGLGSNLLVRDGGVRG
SVVVLHARLNGLQLESDMGQMLIYAGAGVACAKVARFAALQGLGGAEFLAGIPGTVGGALAMNAGCYGTETWDIVSSVQT
IDRLGILRRRPPGNYEIGYRHVALKAEKSSGSQKMGARENAPDDSLTDEWFSGAWFALPRDHAAAVRQKIKELLARRIHT
QPLNLPNAGSVFRNPENDKAARLIESCGLKEFRIGGAMVSPRHANFIVNTGGATASDIEAVIAAVRETVKKQTGVELKQE
VRIIGTPARPELHVSRTAYRKDGGYGG

Specific function: Cell wall formation

COG id: COG0812

COG function: function code M; UDP-N-acetylmuramate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain

Homologues:

Organism=Escherichia coli, GI1790407, Length=302, Percent_Identity=27.4834437086093, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURB_NITMU (Q2Y640)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_413173.1
- ProteinModelPortal:   Q2Y640
- SMR:   Q2Y640
- STRING:   Q2Y640
- GeneID:   3786751
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A2492
- eggNOG:   COG0812
- HOGENOM:   HBG686573
- OMA:   SKKHAGF
- PhylomeDB:   Q2Y640
- ProtClustDB:   PRK13905
- BioCyc:   NMUL323848:NMUL_A2492-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00037
- InterPro:   IPR016169
- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR003170
- InterPro:   IPR011601
- InterPro:   IPR006094
- Gene3D:   G3DSA:3.30.465.10
- Gene3D:   G3DSA:3.30.43.10
- Gene3D:   G3DSA:3.90.78.10
- PANTHER:   PTHR21071
- TIGRFAMs:   TIGR00179

Pfam domain/function: PF01565 FAD_binding_4; PF02873 MurB_C; SSF56176 FAD-binding_2; SSF56194 MurB_C

EC number: =1.1.1.158

Molecular weight: Translated: 37384; Mature: 37384

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: PS51387 FAD_PCMH

Important sites: ACT_SITE 180-180 ACT_SITE 250-250 ACT_SITE 320-320

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDMSEINFSLDYAGVRGELRHDQPMKNYTSWRAGGSAERIYLPGDLPDLAAFLRGLPWNE
CCCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCCEEEECCCCHHHHHHHHCCCCCC
PVYVMGLGSNLLVRDGGVRGSVVVLHARLNGLQLESDMGQMLIYAGAGVACAKVARFAAL
CEEEEEECCCEEEECCCCCCEEEEEEECCCCEEECCCCCCEEEEECCCHHHHHHHHHHHH
QGLGGAEFLAGIPGTVGGALAMNAGCYGTETWDIVSSVQTIDRLGILRRRPPGNYEIGYR
HCCCCHHHHHCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
HVALKAEKSSGSQKMGARENAPDDSLTDEWFSGAWFALPRDHAAAVRQKIKELLARRIHT
EEEEEECCCCCCHHCCCCCCCCCCCCCHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHCC
QPLNLPNAGSVFRNPENDKAARLIESCGLKEFRIGGAMVSPRHANFIVNTGGATASDIEA
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEECCCEEECCCCCCEEEECCCCCHHHHHH
VIAAVRETVKKQTGVELKQEVRIIGTPARPELHVSRTAYRKDGGYGG
HHHHHHHHHHHHCCCCHHHCEEEEECCCCCCEEEHHHHHHCCCCCCC
>Mature Secondary Structure
MDMSEINFSLDYAGVRGELRHDQPMKNYTSWRAGGSAERIYLPGDLPDLAAFLRGLPWNE
CCCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCCEEEECCCCHHHHHHHHCCCCCC
PVYVMGLGSNLLVRDGGVRGSVVVLHARLNGLQLESDMGQMLIYAGAGVACAKVARFAAL
CEEEEEECCCEEEECCCCCCEEEEEEECCCCEEECCCCCCEEEEECCCHHHHHHHHHHHH
QGLGGAEFLAGIPGTVGGALAMNAGCYGTETWDIVSSVQTIDRLGILRRRPPGNYEIGYR
HCCCCHHHHHCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
HVALKAEKSSGSQKMGARENAPDDSLTDEWFSGAWFALPRDHAAAVRQKIKELLARRIHT
EEEEEECCCCCCHHCCCCCCCCCCCCCHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHCC
QPLNLPNAGSVFRNPENDKAARLIESCGLKEFRIGGAMVSPRHANFIVNTGGATASDIEA
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEECCCEEECCCCCCEEEECCCCCHHHHHH
VIAAVRETVKKQTGVELKQEVRIIGTPARPELHVSRTAYRKDGGYGG
HHHHHHHHHHHHCCCCHHHCEEEEECCCCCCEEEHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA