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The map label for this gene is ddl

Identifier: 82703606

GI number: 82703606

Start: 2845713

End: 2846633

Strand: Reverse

Name: ddl

Synonym: Nmul_A2491

Alternate gene names: 82703606

Gene position: 2846633-2845713 (Counterclockwise)

Preceding gene: 82703607

Following gene: 82703605

Centisome position: 89.4

GC content: 57.87

Gene sequence:

>921_bases
ATGGCGGGTAGTCATGACTTCGGGAAGGTCGCGGTTCTCCTCGGAGGACGATCAGCGGAGCGCGAGATTTCCCTTGAAAG
CGGTAAAGCCGTACTCGATGCTCTGCGCAGCTCCGGGGTGGACGCTCATCCATTCGACCCCTCCGAACAGCACATGGACG
CGCTTTTGCAGCAAGGGTATACGCGCGCTCATATCGCCCTCCATGGCCGTTATGGTGAGGACGGAACAGTCCAGGGCGCG
CTGGAACTGCTGGGGATTCCCTATACCGGGAGCGGCGTGCTGGCCAGCGCCTTGGCGATGGACAAATGGCGTACCAAATT
GCTCTGGCAGTCCGCGGGCATCAACACGCCGCGCCATATCCTGCTCGATGAGCAGAGCGATTTTGATGCAGTGGCAAAAG
AGTTGGGCCTGCCGCTGATTGTAAAACCTTCGCGAGAGGGTTCCACCATTGGTCTGAGCAAGGTCAGGGAAGCAGGGGAA
GTCGCTGCCGCATGGCATCTTGCTGCCCGCCATGATGCCATGGTGCTGGCGGAGCAGTTTATCGAAGGAACCGAACTCAC
GGCTTCTATTCTCGGAGATGTAGCGTTACCGCTGGTGCGAATTCAGGTCGAGGGTGATCTGTACGACTACCAAGCCAAAT
ACCTGTCGGACAAGACGCAGTATTTCTGCCCGAGCGGCGTGTCCGAGGAGCAGGAGTGCCTCATTCGGAAACAGGCATTG
CAGGCGCACAGACTGTTGGGCTGCGAAGGTTGGGGAAGGGTGGATTTGATACTGGATAAATCGGGCACTCCCTATTTTCT
CGAAGCGAACACCTCCCCTGGGATGACCACCCATAGTCTCGTTCCCATGGCGGCAAAAGCGGCAGGAATATCCTTCGAGG
AGCTGGTGCTGAAGATACTGGGACTGGCCCATGTGGGATAA

Upstream 100 bases:

>100_bases
CAGACCGGCGTCGAACTGAAACAGGAGGTGCGCATTATCGGGACCCCCGCCAGACCCGAGTTGCATGTCTCCCGGACTGC
TTACAGAAAGGATGGTGGAT

Downstream 100 bases:

>100_bases
CCATCAGGCATTGAACCAGGTGGCGGACTGGCTGTTTACGCTGGCGGGACTGACAACGATCTACCTTATGGTCCAGTGGA
CGATCCATCTGCCGCTGCTG

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MAGSHDFGKVAVLLGGRSAEREISLESGKAVLDALRSSGVDAHPFDPSEQHMDALLQQGYTRAHIALHGRYGEDGTVQGA
LELLGIPYTGSGVLASALAMDKWRTKLLWQSAGINTPRHILLDEQSDFDAVAKELGLPLIVKPSREGSTIGLSKVREAGE
VAAAWHLAARHDAMVLAEQFIEGTELTASILGDVALPLVRIQVEGDLYDYQAKYLSDKTQYFCPSGVSEEQECLIRKQAL
QAHRLLGCEGWGRVDLILDKSGTPYFLEANTSPGMTTHSLVPMAAKAAGISFEELVLKILGLAHVG

Sequences:

>Translated_306_residues
MAGSHDFGKVAVLLGGRSAEREISLESGKAVLDALRSSGVDAHPFDPSEQHMDALLQQGYTRAHIALHGRYGEDGTVQGA
LELLGIPYTGSGVLASALAMDKWRTKLLWQSAGINTPRHILLDEQSDFDAVAKELGLPLIVKPSREGSTIGLSKVREAGE
VAAAWHLAARHDAMVLAEQFIEGTELTASILGDVALPLVRIQVEGDLYDYQAKYLSDKTQYFCPSGVSEEQECLIRKQAL
QAHRLLGCEGWGRVDLILDKSGTPYFLEANTSPGMTTHSLVPMAAKAAGISFEELVLKILGLAHVG
>Mature_305_residues
AGSHDFGKVAVLLGGRSAEREISLESGKAVLDALRSSGVDAHPFDPSEQHMDALLQQGYTRAHIALHGRYGEDGTVQGAL
ELLGIPYTGSGVLASALAMDKWRTKLLWQSAGINTPRHILLDEQSDFDAVAKELGLPLIVKPSREGSTIGLSKVREAGEV
AAAWHLAARHDAMVLAEQFIEGTELTASILGDVALPLVRIQVEGDLYDYQAKYLSDKTQYFCPSGVSEEQECLIRKQALQ
AHRLLGCEGWGRVDLILDKSGTPYFLEANTSPGMTTHSLVPMAAKAAGISFEELVLKILGLAHVG

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=303, Percent_Identity=55.7755775577558, Blast_Score=333, Evalue=7e-93,
Organism=Escherichia coli, GI1786579, Length=351, Percent_Identity=33.3333333333333, Blast_Score=169, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_NITMU (Q2Y641)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_413172.1
- ProteinModelPortal:   Q2Y641
- SMR:   Q2Y641
- STRING:   Q2Y641
- GeneID:   3786750
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A2491
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   SWIVEDA
- PhylomeDB:   Q2Y641
- ProtClustDB:   PRK01372
- BioCyc:   NMUL323848:NMUL_A2491-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 32910; Mature: 32779

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGSHDFGKVAVLLGGRSAEREISLESGKAVLDALRSSGVDAHPFDPSEQHMDALLQQGY
CCCCCCCCEEEEEECCCCCCCEEEHHCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCC
TRAHIALHGRYGEDGTVQGALELLGIPYTGSGVLASALAMDKWRTKLLWQSAGINTPRHI
CEEEEEEECCCCCCCCHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
LLDEQSDFDAVAKELGLPLIVKPSREGSTIGLSKVREAGEVAAAWHLAARHDAMVLAEQF
EECCCCCHHHHHHHCCCCEEEECCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEGTELTASILGDVALPLVRIQVEGDLYDYQAKYLSDKTQYFCPSGVSEEQECLIRKQAL
HCCCHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHH
QAHRLLGCEGWGRVDLILDKSGTPYFLEANTSPGMTTHSLVPMAAKAAGISFEELVLKIL
HHHHHCCCCCCCEEEEEEECCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
GLAHVG
HHHCCC
>Mature Secondary Structure 
AGSHDFGKVAVLLGGRSAEREISLESGKAVLDALRSSGVDAHPFDPSEQHMDALLQQGY
CCCCCCCEEEEEECCCCCCCEEEHHCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCC
TRAHIALHGRYGEDGTVQGALELLGIPYTGSGVLASALAMDKWRTKLLWQSAGINTPRHI
CEEEEEEECCCCCCCCHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
LLDEQSDFDAVAKELGLPLIVKPSREGSTIGLSKVREAGEVAAAWHLAARHDAMVLAEQF
EECCCCCHHHHHHHCCCCEEEECCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEGTELTASILGDVALPLVRIQVEGDLYDYQAKYLSDKTQYFCPSGVSEEQECLIRKQAL
HCCCHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHH
QAHRLLGCEGWGRVDLILDKSGTPYFLEANTSPGMTTHSLVPMAAKAAGISFEELVLKIL
HHHHHCCCCCCCEEEEEEECCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
GLAHVG
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA