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The map label for this gene is folE

Identifier: 82703534

GI number: 82703534

Start: 2756252

End: 2756827

Strand: Direct

Name: folE

Synonym: Nmul_A2419

Alternate gene names: 82703534

Gene position: 2756252-2756827 (Clockwise)

Preceding gene: 82703532

Following gene: 82703535

Centisome position: 86.56

GC content: 60.07

Gene sequence:

>576_bases
ATGGATTCAAATGATTCAGAATTCGGAATCGGCGATTGGCAGCGACTGTTGCGCTCTCTCGGCGAAGACCCCAGCCGCCA
GGGCCTGCGCGAAACGCCGGAGCGCGTGACACGCGCGTGGGCTCACTGGACGCGCGGGTACAAGCAGGATCCGGCAGCGA
TCCTCAAGACGTTCGCGGACGGCGGCGAAAGCTACGACGAATTGATCGTAGTGAGACAGATACCCGTTTACTCGCACTGC
GAACACCACCTGGCTCCCTTTTTTGGCCACGCAACCATCGGGTATCTCCCCACGGGTCATATCGTCGGGTTGTCCAAACT
GACGCGACTGGTTAACTGTTTTGCAGCTCGCCTGCAGGTACAGGAACGCCTGACTCAGCAGGTGGCACAATCCCTGCTGG
AGCACCTGCAACCGAAGGCGGTCGGCGTCATTCTCCGCTGCCGCCACATGTGCATGGAATCGAGGGGAATTGCCGTTGCC
GGGGAAGAAACGGTAACGTCGGCCATGCTGGGCGACCTGAAAACGAATGCAGCGCAGCGTGCCGAGTTCCTGGCATTGGC
CGAGTCGCACGAGTAA

Upstream 100 bases:

>100_bases
GGCACTCGCAGCGCACTTCAGCTGGGGTTCGCTGACAGTGAGATGGGACCGATATGTGGCTTATACATATCCCTCTCGAT
AGAATAAAGGCGTGGTGAAC

Downstream 100 bases:

>100_bases
TCCAAGGAGTCGCTGAACACTTTTTCCGAAAAAAACACTCCGCTTCCCAGGTGTTGATTGGCATACTGGGATTCCCAGGG
AGCGCCGCTCCAATACCCTG

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MDSNDSEFGIGDWQRLLRSLGEDPSRQGLRETPERVTRAWAHWTRGYKQDPAAILKTFADGGESYDELIVVRQIPVYSHC
EHHLAPFFGHATIGYLPTGHIVGLSKLTRLVNCFAARLQVQERLTQQVAQSLLEHLQPKAVGVILRCRHMCMESRGIAVA
GEETVTSAMLGDLKTNAAQRAEFLALAESHE

Sequences:

>Translated_191_residues
MDSNDSEFGIGDWQRLLRSLGEDPSRQGLRETPERVTRAWAHWTRGYKQDPAAILKTFADGGESYDELIVVRQIPVYSHC
EHHLAPFFGHATIGYLPTGHIVGLSKLTRLVNCFAARLQVQERLTQQVAQSLLEHLQPKAVGVILRCRHMCMESRGIAVA
GEETVTSAMLGDLKTNAAQRAEFLALAESHE
>Mature_191_residues
MDSNDSEFGIGDWQRLLRSLGEDPSRQGLRETPERVTRAWAHWTRGYKQDPAAILKTFADGGESYDELIVVRQIPVYSHC
EHHLAPFFGHATIGYLPTGHIVGLSKLTRLVNCFAARLQVQERLTQQVAQSLLEHLQPKAVGVILRCRHMCMESRGIAVA
GEETVTSAMLGDLKTNAAQRAEFLALAESHE

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=189, Percent_Identity=43.9153439153439, Blast_Score=167, Evalue=5e-42,
Organism=Homo sapiens, GI4503949, Length=189, Percent_Identity=43.9153439153439, Blast_Score=167, Evalue=5e-42,
Organism=Homo sapiens, GI66932970, Length=158, Percent_Identity=41.1392405063291, Blast_Score=132, Evalue=1e-31,
Organism=Homo sapiens, GI66932972, Length=145, Percent_Identity=44.1379310344828, Blast_Score=131, Evalue=4e-31,
Organism=Escherichia coli, GI1788476, Length=184, Percent_Identity=34.2391304347826, Blast_Score=102, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17560486, Length=174, Percent_Identity=43.6781609195402, Blast_Score=166, Evalue=8e-42,
Organism=Saccharomyces cerevisiae, GI6321706, Length=176, Percent_Identity=47.1590909090909, Blast_Score=169, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24656782, Length=177, Percent_Identity=47.4576271186441, Blast_Score=167, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24656796, Length=177, Percent_Identity=47.4576271186441, Blast_Score=167, Evalue=4e-42,
Organism=Drosophila melanogaster, GI24656791, Length=177, Percent_Identity=47.4576271186441, Blast_Score=167, Evalue=5e-42,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): GCH1_NITMU (Q2Y6B3)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_413100.1
- ProteinModelPortal:   Q2Y6B3
- SMR:   Q2Y6B3
- STRING:   Q2Y6B3
- GeneID:   3785511
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A2419
- eggNOG:   COG0302
- HOGENOM:   HBG370191
- OMA:   VTQTQAE
- PhylomeDB:   Q2Y6B3
- ProtClustDB:   CLSK943213
- BioCyc:   NMUL323848:NMUL_A2419-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234
- TIGRFAMs:   TIGR00063

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =3.5.4.16

Molecular weight: Translated: 21304; Mature: 21304

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSNDSEFGIGDWQRLLRSLGEDPSRQGLRETPERVTRAWAHWTRGYKQDPAAILKTFAD
CCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHC
GGESYDELIVVRQIPVYSHCEHHLAPFFGHATIGYLPTGHIVGLSKLTRLVNCFAARLQV
CCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
QERLTQQVAQSLLEHLQPKAVGVILRCRHMCMESRGIAVAGEETVTSAMLGDLKTNAAQR
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHH
AEFLALAESHE
HHHHHHHHCCC
>Mature Secondary Structure
MDSNDSEFGIGDWQRLLRSLGEDPSRQGLRETPERVTRAWAHWTRGYKQDPAAILKTFAD
CCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHC
GGESYDELIVVRQIPVYSHCEHHLAPFFGHATIGYLPTGHIVGLSKLTRLVNCFAARLQV
CCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
QERLTQQVAQSLLEHLQPKAVGVILRCRHMCMESRGIAVAGEETVTSAMLGDLKTNAAQR
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHH
AEFLALAESHE
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA