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The map label for this gene is pmbA [H]

Identifier: 82703530

GI number: 82703530

Start: 2751284

End: 2752642

Strand: Direct

Name: pmbA [H]

Synonym: Nmul_A2415

Alternate gene names: 82703530

Gene position: 2751284-2752642 (Clockwise)

Preceding gene: 82703528

Following gene: 82703531

Centisome position: 86.4

GC content: 56.07

Gene sequence:

>1359_bases
ATGAATGATAACGCCCTGCCTTCACAACGTTTTTCCTATTCGTTTTCAGTGCTTCAGCAAATCGCGCAGGATGTCCTGAA
TCACGCGCGCAAAGGGGGCGCAAGCGCCTGCGAAACCGATGTTTCAGATGGTTTTGGCCAGAATGTCACGGTGCGGCGGG
GGCAGGTGGAAACGATAGAGTACAACCGCGACAAGGGATTGTCAGTAACAGTTTATGTGGGGCAAAAGCGCGGGCATGCC
AGCACTTCGGATTTTTCGCCACAGGCGATAAGCGATACGGTTACGGCGGCGCTTTCGATTGCCCGCCATACCGCCGCCGA
TGACTGCGCAGGTCTGGCGGATCCCGACTTGCTGGCACGCGAATTTCCCGATCTCGAATTATATTTTCCCTGGGACTTGC
CGGTGGAGCAGGCAATAGAATTGGCGCAAGCCTGCGAAGCAGCCGCATTTGCAGTCGATAAGCGCATTACCAATTCCGAA
GGAGCCAGTGTCTCGCTGGGCGAATCGCAGTTTGTCTATGCAAACAGTCTGGGATTCATGGGAGGCTATCCTTCCTCCCG
CCACAGTATCAGCTGTGCGATGATTGCTAGCCACGAGGACAGCATGCAGCGTGACTACTGGTACAGCGTGGCGCGCGATG
CTTCCGACCTGGAGCATGCCGCTGCGGTGGGGAAGAAAGCCGGAACACGGAGTGTCGGGCGTCTGGGTGCGAGAAAAATC
GCTACCTGCGAAGTTCCCGTTCTGTTCGAGGCGCCCATTGCCTCCAGCCTGATCGGGCATTTCGTCGGGGCAGTGAGCGG
AGGCAGTCTTTACCGCAAATCCTCATTTCTCCTCGACAGTATCGGCAAGCAGATATTTGCGCCGGGCATCCAGATACGCG
AACGGCCGCATCTGAAAAAAGGCCTGGGCAGCGGGCCTTTCGATAATGAAGGGGTAGCGACCCGGGACCGTAACATCGTG
GAAAATGGGGTGGTACAAGGCTACTTTCTCGGCAGTTACTCCGCCCGCAAACTAGGGCTTGTCACAACCGGAAATGCCGG
TGGCAATCATAACCTGGTCCTGGACAATGGCGCCGGCCTGACTTTTGCCGAACTGCTCAGGAAAATGGGTAAAGGCGTGC
TCGTGACCGAGTTGCTCGGCCACGGCGTAAACAATGTCACCGGTGATTACTCCAGGGGGGCGGCGGGATACTGGGTCGAA
GACGGAGAAATCCGTTATCCGGTTGAAGAAATAACGATTGCCGGAAATCTGAAAGACATGTTGCCTGGCATTTCCGCTGC
GGGGAATGATGTCATCGTGCGTGGTTCCAAGCAATGCGGTTCATTGCTCATTGATCGAATGATGATTGCGGGCGGCTAG

Upstream 100 bases:

>100_bases
GACTCCTCGCGGGCTGCTCCCCGGTCCTGTGGGCAAGTTGCAGTGCCGGACAGCTACTCAATATAATCCTACAATCCCTG
AATTCCCTGAAGTCACGCCT

Downstream 100 bases:

>100_bases
GCGTTCCGGTCGAACGTGGAACTCCTTGATTTCGCCTTTCCTCCCTATCTCGGTCGGATACCGCCGCGTCTTTTTCTCGC
CTTTCCATTTGCCGTTTCTG

Product: peptidase U62, modulator of DNA gyrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MNDNALPSQRFSYSFSVLQQIAQDVLNHARKGGASACETDVSDGFGQNVTVRRGQVETIEYNRDKGLSVTVYVGQKRGHA
STSDFSPQAISDTVTAALSIARHTAADDCAGLADPDLLAREFPDLELYFPWDLPVEQAIELAQACEAAAFAVDKRITNSE
GASVSLGESQFVYANSLGFMGGYPSSRHSISCAMIASHEDSMQRDYWYSVARDASDLEHAAAVGKKAGTRSVGRLGARKI
ATCEVPVLFEAPIASSLIGHFVGAVSGGSLYRKSSFLLDSIGKQIFAPGIQIRERPHLKKGLGSGPFDNEGVATRDRNIV
ENGVVQGYFLGSYSARKLGLVTTGNAGGNHNLVLDNGAGLTFAELLRKMGKGVLVTELLGHGVNNVTGDYSRGAAGYWVE
DGEIRYPVEEITIAGNLKDMLPGISAAGNDVIVRGSKQCGSLLIDRMMIAGG

Sequences:

>Translated_452_residues
MNDNALPSQRFSYSFSVLQQIAQDVLNHARKGGASACETDVSDGFGQNVTVRRGQVETIEYNRDKGLSVTVYVGQKRGHA
STSDFSPQAISDTVTAALSIARHTAADDCAGLADPDLLAREFPDLELYFPWDLPVEQAIELAQACEAAAFAVDKRITNSE
GASVSLGESQFVYANSLGFMGGYPSSRHSISCAMIASHEDSMQRDYWYSVARDASDLEHAAAVGKKAGTRSVGRLGARKI
ATCEVPVLFEAPIASSLIGHFVGAVSGGSLYRKSSFLLDSIGKQIFAPGIQIRERPHLKKGLGSGPFDNEGVATRDRNIV
ENGVVQGYFLGSYSARKLGLVTTGNAGGNHNLVLDNGAGLTFAELLRKMGKGVLVTELLGHGVNNVTGDYSRGAAGYWVE
DGEIRYPVEEITIAGNLKDMLPGISAAGNDVIVRGSKQCGSLLIDRMMIAGG
>Mature_452_residues
MNDNALPSQRFSYSFSVLQQIAQDVLNHARKGGASACETDVSDGFGQNVTVRRGQVETIEYNRDKGLSVTVYVGQKRGHA
STSDFSPQAISDTVTAALSIARHTAADDCAGLADPDLLAREFPDLELYFPWDLPVEQAIELAQACEAAAFAVDKRITNSE
GASVSLGESQFVYANSLGFMGGYPSSRHSISCAMIASHEDSMQRDYWYSVARDASDLEHAAAVGKKAGTRSVGRLGARKI
ATCEVPVLFEAPIASSLIGHFVGAVSGGSLYRKSSFLLDSIGKQIFAPGIQIRERPHLKKGLGSGPFDNEGVATRDRNIV
ENGVVQGYFLGSYSARKLGLVTTGNAGGNHNLVLDNGAGLTFAELLRKMGKGVLVTELLGHGVNNVTGDYSRGAAGYWVE
DGEIRYPVEEITIAGNLKDMLPGISAAGNDVIVRGSKQCGSLLIDRMMIAGG

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=435, Percent_Identity=50.5747126436782, Blast_Score=437, Evalue=1e-124,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 48033; Mature: 48033

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDNALPSQRFSYSFSVLQQIAQDVLNHARKGGASACETDVSDGFGQNVTVRRGQVETIE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCEEEECCEEEEEE
YNRDKGLSVTVYVGQKRGHASTSDFSPQAISDTVTAALSIARHTAADDCAGLADPDLLAR
ECCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHH
EFPDLELYFPWDLPVEQAIELAQACEAAAFAVDKRITNSEGASVSLGESQFVYANSLGFM
HCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEECCCCCC
GGYPSSRHSISCAMIASHEDSMQRDYWYSVARDASDLEHAAAVGKKAGTRSVGRLGARKI
CCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHCCCEE
ATCEVPVLFEAPIASSLIGHFVGAVSGGSLYRKSSFLLDSIGKQIFAPGIQIRERPHLKK
EEECCCEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
GLGSGPFDNEGVATRDRNIVENGVVQGYFLGSYSARKLGLVTTGNAGGNHNLVLDNGAGL
CCCCCCCCCCCCCCCCCHHHHCCCEEEEEECCCCCCEEEEEEECCCCCCCCEEEECCCCC
TFAELLRKMGKGVLVTELLGHGVNNVTGDYSRGAAGYWVEDGEIRYPVEEITIAGNLKDM
HHHHHHHHHCCCEEHHHHHCCCCCCCCCCCCCCCCCEEEECCEEECCHHHEEEECCHHHH
LPGISAAGNDVIVRGSKQCGSLLIDRMMIAGG
CCCCCCCCCCEEEECCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNDNALPSQRFSYSFSVLQQIAQDVLNHARKGGASACETDVSDGFGQNVTVRRGQVETIE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCEEEECCEEEEEE
YNRDKGLSVTVYVGQKRGHASTSDFSPQAISDTVTAALSIARHTAADDCAGLADPDLLAR
ECCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHH
EFPDLELYFPWDLPVEQAIELAQACEAAAFAVDKRITNSEGASVSLGESQFVYANSLGFM
HCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEECCCCCC
GGYPSSRHSISCAMIASHEDSMQRDYWYSVARDASDLEHAAAVGKKAGTRSVGRLGARKI
CCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHCCCEE
ATCEVPVLFEAPIASSLIGHFVGAVSGGSLYRKSSFLLDSIGKQIFAPGIQIRERPHLKK
EEECCCEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
GLGSGPFDNEGVATRDRNIVENGVVQGYFLGSYSARKLGLVTTGNAGGNHNLVLDNGAGL
CCCCCCCCCCCCCCCCCHHHHCCCEEEEEECCCCCCEEEEEEECCCCCCCCEEEECCCCC
TFAELLRKMGKGVLVTELLGHGVNNVTGDYSRGAAGYWVEDGEIRYPVEEITIAGNLKDM
HHHHHHHHHCCCEEHHHHHCCCCCCCCCCCCCCCCCEEEECCEEECCHHHEEEECCHHHH
LPGISAAGNDVIVRGSKQCGSLLIDRMMIAGG
CCCCCCCCCCEEEECCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]