Click here to switch to the map view.

The map label for this gene is lasI [H]

Identifier: 82703505

GI number: 82703505

Start: 2721910

End: 2722521

Strand: Reverse

Name: lasI [H]

Synonym: Nmul_A2390

Alternate gene names: 82703505

Gene position: 2722521-2721910 (Counterclockwise)

Preceding gene: 82703506

Following gene: 82703504

Centisome position: 85.5

GC content: 53.27

Gene sequence:

>612_bases
ATGCTTGCACAACATGGCAATGGTTCTCTCGATAAACGGGCCGCAATGGGAATGTATCGGCTGAGGCACGAAGTTTTTCA
CGACAGGCTCGGCTGGGAAGTAACCACGGATAACGGGATGGAGCATGACGAGTTCGATCTGGCAGATCCCGTATATGTTC
TGGCAAAAGGGGATGAGGGGGATGAAGACGAGGTGCTCGGTTGCTGGCGACTGCTTCCCACCCAGGGACCCTACATGTTG
AAGGATACTTTTCCGCAACTTCTGCACGGACAGCCCGCGCCGCAGCAATCGGATGTCTGGGAATTGAGCCGTTTTGCCGT
CATGGTCCCCAAATATGAGAGTGCCGGTTTCGGTTTTAGCGAGATTCCGGTAAAAATGATGCAAACCCTTTTTCTCTTCG
CGCGCCACAACAAGATAAAGCGCTATGTAACCGTTACCACCGTCGCAGTGGAGAGACTGATACGCAAGCTTGGCGTGAAC
GTTTCCAGGTTGGGGGCACCGGTCAAAATCGGGCGGGTTTTGACGGTCGCATGCTATATCGAAATCGATGCAATCACCGA
ATTCGCCCTTTTTGGGATACTCCCGGAAAACGCGCAAAGGAAGGCCGCATGA

Upstream 100 bases:

>100_bases
CCGTCTGGTCCTGTAAGATTTTACAGCTATTCGGAGATCCGGTTCCGTTGTTTCATAACGTCGTCATCAACCAACGACGA
GTGAAAAAATGGGACAGATT

Downstream 100 bases:

>100_bases
ATATTCTACTGGTTGGAGAGAGACAAGATACCCGCCAGCTCCTGCAACTGCTGCTTGAAAAATCCAAGCACAGGCTGGAG
CGAAAAACACTGGACAGCTT

Product: autoinducer synthesis protein

Products: NA

Alternate protein names: Autoinducer synthesis protein lasI [H]

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MLAQHGNGSLDKRAAMGMYRLRHEVFHDRLGWEVTTDNGMEHDEFDLADPVYVLAKGDEGDEDEVLGCWRLLPTQGPYML
KDTFPQLLHGQPAPQQSDVWELSRFAVMVPKYESAGFGFSEIPVKMMQTLFLFARHNKIKRYVTVTTVAVERLIRKLGVN
VSRLGAPVKIGRVLTVACYIEIDAITEFALFGILPENAQRKAA

Sequences:

>Translated_203_residues
MLAQHGNGSLDKRAAMGMYRLRHEVFHDRLGWEVTTDNGMEHDEFDLADPVYVLAKGDEGDEDEVLGCWRLLPTQGPYML
KDTFPQLLHGQPAPQQSDVWELSRFAVMVPKYESAGFGFSEIPVKMMQTLFLFARHNKIKRYVTVTTVAVERLIRKLGVN
VSRLGAPVKIGRVLTVACYIEIDAITEFALFGILPENAQRKAA
>Mature_203_residues
MLAQHGNGSLDKRAAMGMYRLRHEVFHDRLGWEVTTDNGMEHDEFDLADPVYVLAKGDEGDEDEVLGCWRLLPTQGPYML
KDTFPQLLHGQPAPQQSDVWELSRFAVMVPKYESAGFGFSEIPVKMMQTLFLFARHNKIKRYVTVTTVAVERLIRKLGVN
VSRLGAPVKIGRVLTVACYIEIDAITEFALFGILPENAQRKAA

Specific function: Required for the synthesis of PAI consisting of 3-oxo-N- (tetrahydro-2-oxo-3-furanyl)-dodecanamide also known as N-(3- oxododecanoyl)homoserine lactone, an autoinducer molecule which binds to lasR and thus acts in elastase biosynthesis regulation [H]

COG id: COG3916

COG function: function code TQ; N-acyl-L-homoserine lactone synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the autoinducer synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016181
- InterPro:   IPR001690
- InterPro:   IPR018311 [H]

Pfam domain/function: PF00765 Autoind_synth [H]

EC number: =2.3.1.184 [H]

Molecular weight: Translated: 22895; Mature: 22895

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00949 AUTOINDUCERS_SYNTH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAQHGNGSLDKRAAMGMYRLRHEVFHDRLGWEVTTDNGMEHDEFDLADPVYVLAKGDEG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCEEEEEECCCC
DEDEVLGCWRLLPTQGPYMLKDTFPQLLHGQPAPQQSDVWELSRFAVMVPKYESAGFGFS
CHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHEEEECCCCCCCCCHH
EIPVKMMQTLFLFARHNKIKRYVTVTTVAVERLIRKLGVNVSRLGAPVKIGRVLTVACYI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEHHHEEEEEEEE
EIDAITEFALFGILPENAQRKAA
EHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLAQHGNGSLDKRAAMGMYRLRHEVFHDRLGWEVTTDNGMEHDEFDLADPVYVLAKGDEG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCEEEEEECCCC
DEDEVLGCWRLLPTQGPYMLKDTFPQLLHGQPAPQQSDVWELSRFAVMVPKYESAGFGFS
CHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHEEEECCCCCCCCCHH
EIPVKMMQTLFLFARHNKIKRYVTVTTVAVERLIRKLGVNVSRLGAPVKIGRVLTVACYI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEHHHEEEEEEEE
EIDAITEFALFGILPENAQRKAA
EHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8493556; 10984043; 8278364 [H]