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The map label for this gene is rlmN

Identifier: 82703494

GI number: 82703494

Start: 2706455

End: 2707552

Strand: Reverse

Name: rlmN

Synonym: Nmul_A2379

Alternate gene names: 82703494

Gene position: 2707552-2706455 (Counterclockwise)

Preceding gene: 82703495

Following gene: 82703493

Centisome position: 85.03

GC content: 57.01

Gene sequence:

>1098_bases
ATGAGCATCAATCTTCTGGATTTTGATGCGAAAGGGCTTACCGGTTTCTGTGCGGAAATCGGTGAGAAGCCGTTCCGCGC
CCGTCAACTGCTGCGCTGGATACACCGGACCGGTGAAGCCGATTTCGATGCCATGAGCGACCTCGCGAAAGGATTGCGGG
AAAAGCTGGCGGCTGCCGCCGTGATAGAACCGCCGAAAGTCATCAGCGATCATACCGCTTCCGATGGTACTCGCAAATGG
CTGCTGTCGGTCGGCGCGGGTAATGGAATCGAAACAGTCTACATACCGGAAACGAGCCGGGGGACGCTGTGTATTTCCAG
CCAGGTAGGGTGTGCGCTCGCATGCGCATTTTGTTCCACCGGGAGGCAAGGGTTCAACCGTAATCTGACGGTGGCGGAAA
TCATCGGCCAATTGTGGTGGGCGAATAAAGCGCTGACGGAAACGTTCACGAGCGAGGCGGGACGCGAGCGTCCCATCACC
AATATAGTCATGATGGGGATGGGAGAGCCGCTGACGAATTTCGAAAATGTAGTGACCTCGCTCGACCTGATGCTGGACGA
CAATGCTTATGGCTTATCACGGCGCCGGGTGACCGTGAGCACGTCGGGAATAATCCCGGCCATGGACCGTCTCCGCGAGC
GTTGCCCCGTCGCGCTGGCGGTATCCCTGCATGCTCCCAACGACGCGTTGCGCGATCAATTGGTGCCGATCAACAGGAAA
TACCCGATCAGGGAACTGCTGGGCGCATGCGAGCGCTATCTTCAATCCGCACCCCGAGATTTCATCACTTTTGAATATGT
CATGCTGGATGGCGTGAATGACAGCGTGGCGCAAGCGCGTGAATTGGTGCAACTGGTAAGGGACATTCCCTGCAAGTTAA
ACCTGATTCCGTTCAATCCTTTTCCTGATTCAGGTTTCAGGCGTTCTTCCGCAAACGCCGTATCCCGCTTTCGCGATGTG
TTGATGGAGGCAGGATTGGTGACTACGGTACGCAAGACGCGGGGAGATGATATTGCCGCGGCCTGCGGCCAGCTCGCGGG
AAAAGTCCTCGACAAGACACGCCGCGTCCCCCGCAACATTGCGGAAGCAGCTGGATGA

Upstream 100 bases:

>100_bases
GTGCATGGCTCGGATGCACCTGAAACAGCGGCGGTTGAGATCGCCTATTTTTTTCCGGAACTCAACATTTATACGCGATA
AGGGGGATCACGGCTCAGGT

Downstream 100 bases:

>100_bases
GCAGGTTGGGGCGTATTTTCCCGGTCGGCAACTGCACCTGAAGGCGTGAAGGCGTAAAAGCGATTGCGGATATGAAGCGT
CAGGTAACATGAGATGGGGT

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MSINLLDFDAKGLTGFCAEIGEKPFRARQLLRWIHRTGEADFDAMSDLAKGLREKLAAAAVIEPPKVISDHTASDGTRKW
LLSVGAGNGIETVYIPETSRGTLCISSQVGCALACAFCSTGRQGFNRNLTVAEIIGQLWWANKALTETFTSEAGRERPIT
NIVMMGMGEPLTNFENVVTSLDLMLDDNAYGLSRRRVTVSTSGIIPAMDRLRERCPVALAVSLHAPNDALRDQLVPINRK
YPIRELLGACERYLQSAPRDFITFEYVMLDGVNDSVAQARELVQLVRDIPCKLNLIPFNPFPDSGFRRSSANAVSRFRDV
LMEAGLVTTVRKTRGDDIAAACGQLAGKVLDKTRRVPRNIAEAAG

Sequences:

>Translated_365_residues
MSINLLDFDAKGLTGFCAEIGEKPFRARQLLRWIHRTGEADFDAMSDLAKGLREKLAAAAVIEPPKVISDHTASDGTRKW
LLSVGAGNGIETVYIPETSRGTLCISSQVGCALACAFCSTGRQGFNRNLTVAEIIGQLWWANKALTETFTSEAGRERPIT
NIVMMGMGEPLTNFENVVTSLDLMLDDNAYGLSRRRVTVSTSGIIPAMDRLRERCPVALAVSLHAPNDALRDQLVPINRK
YPIRELLGACERYLQSAPRDFITFEYVMLDGVNDSVAQARELVQLVRDIPCKLNLIPFNPFPDSGFRRSSANAVSRFRDV
LMEAGLVTTVRKTRGDDIAAACGQLAGKVLDKTRRVPRNIAEAAG
>Mature_364_residues
SINLLDFDAKGLTGFCAEIGEKPFRARQLLRWIHRTGEADFDAMSDLAKGLREKLAAAAVIEPPKVISDHTASDGTRKWL
LSVGAGNGIETVYIPETSRGTLCISSQVGCALACAFCSTGRQGFNRNLTVAEIIGQLWWANKALTETFTSEAGRERPITN
IVMMGMGEPLTNFENVVTSLDLMLDDNAYGLSRRRVTVSTSGIIPAMDRLRERCPVALAVSLHAPNDALRDQLVPINRKY
PIRELLGACERYLQSAPRDFITFEYVMLDGVNDSVAQARELVQLVRDIPCKLNLIPFNPFPDSGFRRSSANAVSRFRDVL
MEAGLVTTVRKTRGDDIAAACGQLAGKVLDKTRRVPRNIAEAAG

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=359, Percent_Identity=54.5961002785515, Blast_Score=407, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_NITMU (Q2Y6F3)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_413060.1
- ProteinModelPortal:   Q2Y6F3
- STRING:   Q2Y6F3
- GeneID:   3784970
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A2379
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- PhylomeDB:   Q2Y6F3
- ProtClustDB:   CLSK585184
- BioCyc:   NMUL323848:NMUL_A2379-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 40003; Mature: 39872

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSINLLDFDAKGLTGFCAEIGEKPFRARQLLRWIHRTGEADFDAMSDLAKGLREKLAAAA
CCCEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
VIEPPKVISDHTASDGTRKWLLSVGAGNGIETVYIPETSRGTLCISSQVGCALACAFCST
HCCCCHHHCCCCCCCCHHEEEEEECCCCCEEEEEECCCCCCCEEEECCCHHHHHHHHHHC
GRQGFNRNLTVAEIIGQLWWANKALTETFTSEAGRERPITNIVMMGMGEPLTNFENVVTS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHEECCCCCCHHHHHHHHH
LDLMLDDNAYGLSRRRVTVSTSGIIPAMDRLRERCPVALAVSLHAPNDALRDQLVPINRK
HHHEECCCCCCCCCCEEEEECCCCHHHHHHHHHHCCEEEEEEEECCCHHHHHCCCCCCCC
YPIRELLGACERYLQSAPRDFITFEYVMLDGVNDSVAQARELVQLVRDIPCKLNLIPFNP
CCHHHHHHHHHHHHHHCCCHHEEEEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCC
FPDSGFRRSSANAVSRFRDVLMEAGLVTTVRKTRGDDIAAACGQLAGKVLDKTRRVPRNI
CCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
AEAAG
HHHCC
>Mature Secondary Structure 
SINLLDFDAKGLTGFCAEIGEKPFRARQLLRWIHRTGEADFDAMSDLAKGLREKLAAAA
CCEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
VIEPPKVISDHTASDGTRKWLLSVGAGNGIETVYIPETSRGTLCISSQVGCALACAFCST
HCCCCHHHCCCCCCCCHHEEEEEECCCCCEEEEEECCCCCCCEEEECCCHHHHHHHHHHC
GRQGFNRNLTVAEIIGQLWWANKALTETFTSEAGRERPITNIVMMGMGEPLTNFENVVTS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHEECCCCCCHHHHHHHHH
LDLMLDDNAYGLSRRRVTVSTSGIIPAMDRLRERCPVALAVSLHAPNDALRDQLVPINRK
HHHEECCCCCCCCCCEEEEECCCCHHHHHHHHHHCCEEEEEEEECCCHHHHHCCCCCCCC
YPIRELLGACERYLQSAPRDFITFEYVMLDGVNDSVAQARELVQLVRDIPCKLNLIPFNP
CCHHHHHHHHHHHHHHCCCHHEEEEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCC
FPDSGFRRSSANAVSRFRDVLMEAGLVTTVRKTRGDDIAAACGQLAGKVLDKTRRVPRNI
CCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
AEAAG
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA