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The map label for this gene is ispG [H]

Identifier: 82703492

GI number: 82703492

Start: 2703718

End: 2704968

Strand: Reverse

Name: ispG [H]

Synonym: Nmul_A2377

Alternate gene names: 82703492

Gene position: 2704968-2703718 (Counterclockwise)

Preceding gene: 82703493

Following gene: 82703491

Centisome position: 84.95

GC content: 58.67

Gene sequence:

>1251_bases
ATGTCTATGGTTCAAAGTGCTTTTCCCCCACGCCGGAACAGCGTGGGCGTTCAGGTAGGTTCGATCCGGATCGGCGGGGG
CGCGCCCATCGTAGTGCAGTCCATGACCAATACCGATACGGAAGATGAAATCGCTACCACCGTGCAAGTGGCCCAGCTTG
CGCGTGCCGGATCCGAACTCGTGCGCATCACCGTCAATACGGCCGAAGCAGCCAGGGCGGTGCCGGGTATCAGGGCGCGA
CTCGACGATATGGGTTGCCAAGTTCCCCTGATAGGCGATTTTCACTTCAATGGCCATAAACTCGTGACTGAGTATCCCGG
TTGCGCCCGTGCGCTGGCGAAATACCGTATCAATCCCGGTAATGTCGGGCACGGAAAGAAACGTGACGAACAGTTTTCCA
TTCTGATCGAAGCGGCCTGCAAGTATGAAAAACCGGTGCGCATCGGGGTCAACTGGGGAAGCCTCGATCCAGAGCTGCTG
GCGCGCATGATGGACGAGAATGCCCGCTCCGGGGACCCGAGGGATGCCTCCGCGGTAATGTACGAAGCCTTGATTACCTC
TGCGCTTCAAAGCGCTGAGCGTGCGGAGGAGATCGGGCTGGGGCGTGACAGAATCATATTGTCATGCAAGATGAGCGGCG
TGAGAGACCTCATTACCGTTTATCGCGCCCTTGCGGCCCGCTGCGATTATGCGCTGCACCTGGGGCTCACCGAGGCGGGC
ATGGGTTCGAAGGGGATTGTTGCTTCCACGGCGGCATTGTCGGTACTGCTTCTCGAAGGTATCGGCGATACGATACGGAT
ATCGTTGACGCCCGAACCAGGCGGAGACCGCGCGCGCGAAGTGGTCGTGGCCCAGGAGATACTGCAAACCACCGGTTTGC
GCGCTTTTGTGCCTCTGGTTGCCGCCTGTCCCGGCTGCGGCCGTACCACCAGCACCTATTTTCAGGAGCTGGCGGAAAGC
ATCCAGGGCTACGTGCGCGAGCAGATGCTGGTATGGCGCGAGGAATACGAAGGTGTGGAAAATATGACCCTCGCTGTGAT
GGGGTGCGTGGTCAATGGTCCCGGCGAAAGCAAGCATGCCAATATCGGCATCAGCCTGCCGGGCTCGGGGGAACGGCCTG
TGGCGCCGGTATTTGTGGATGGCCAGAAGGCTGTAACGCTGAAGGGCGATAATATTGCAGGAGAGTTTCGCCAGATAGTC
GATGAATATGTGCAGATGAAATACCCCAAGAAAGCAGTCGATGCCCACTAG

Upstream 100 bases:

>100_bases
CAAACCAGTCGATCTCTCGATTTTCGCCAAACTTCCCGGTGGAATGGCACATCTCGTGCTCCAATAGATTTCAATTTCAA
CACGACATCTCATATTCATC

Downstream 100 bases:

>100_bases
GGGGATTCAGGCTGTACGCGGGATGAACGACATCCTGCCCGATCAGATTGACCGGTGGGAGTTCTTCGAGCAAAGTGTCC
GCGACTGGATGGCGGCTTAT

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MSMVQSAFPPRRNSVGVQVGSIRIGGGAPIVVQSMTNTDTEDEIATTVQVAQLARAGSELVRITVNTAEAARAVPGIRAR
LDDMGCQVPLIGDFHFNGHKLVTEYPGCARALAKYRINPGNVGHGKKRDEQFSILIEAACKYEKPVRIGVNWGSLDPELL
ARMMDENARSGDPRDASAVMYEALITSALQSAERAEEIGLGRDRIILSCKMSGVRDLITVYRALAARCDYALHLGLTEAG
MGSKGIVASTAALSVLLLEGIGDTIRISLTPEPGGDRAREVVVAQEILQTTGLRAFVPLVAACPGCGRTTSTYFQELAES
IQGYVREQMLVWREEYEGVENMTLAVMGCVVNGPGESKHANIGISLPGSGERPVAPVFVDGQKAVTLKGDNIAGEFRQIV
DEYVQMKYPKKAVDAH

Sequences:

>Translated_416_residues
MSMVQSAFPPRRNSVGVQVGSIRIGGGAPIVVQSMTNTDTEDEIATTVQVAQLARAGSELVRITVNTAEAARAVPGIRAR
LDDMGCQVPLIGDFHFNGHKLVTEYPGCARALAKYRINPGNVGHGKKRDEQFSILIEAACKYEKPVRIGVNWGSLDPELL
ARMMDENARSGDPRDASAVMYEALITSALQSAERAEEIGLGRDRIILSCKMSGVRDLITVYRALAARCDYALHLGLTEAG
MGSKGIVASTAALSVLLLEGIGDTIRISLTPEPGGDRAREVVVAQEILQTTGLRAFVPLVAACPGCGRTTSTYFQELAES
IQGYVREQMLVWREEYEGVENMTLAVMGCVVNGPGESKHANIGISLPGSGERPVAPVFVDGQKAVTLKGDNIAGEFRQIV
DEYVQMKYPKKAVDAH
>Mature_415_residues
SMVQSAFPPRRNSVGVQVGSIRIGGGAPIVVQSMTNTDTEDEIATTVQVAQLARAGSELVRITVNTAEAARAVPGIRARL
DDMGCQVPLIGDFHFNGHKLVTEYPGCARALAKYRINPGNVGHGKKRDEQFSILIEAACKYEKPVRIGVNWGSLDPELLA
RMMDENARSGDPRDASAVMYEALITSALQSAERAEEIGLGRDRIILSCKMSGVRDLITVYRALAARCDYALHLGLTEAGM
GSKGIVASTAALSVLLLEGIGDTIRISLTPEPGGDRAREVVVAQEILQTTGLRAFVPLVAACPGCGRTTSTYFQELAESI
QGYVREQMLVWREEYEGVENMTLAVMGCVVNGPGESKHANIGISLPGSGERPVAPVFVDGQKAVTLKGDNIAGEFRQIVD
EYVQMKYPKKAVDAH

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=358, Percent_Identity=36.3128491620112, Blast_Score=183, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 44672; Mature: 44541

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMVQSAFPPRRNSVGVQVGSIRIGGGAPIVVQSMTNTDTEDEIATTVQVAQLARAGSEL
CCCCHHCCCCCCCCCCEEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCEE
VRITVNTAEAARAVPGIRARLDDMGCQVPLIGDFHFNGHKLVTEYPGCARALAKYRINPG
EEEEEECHHHHHHCCCHHHHHHHCCCCEEEEEEEEECCCEEEECCCHHHHHHHHHCCCCC
NVGHGKKRDEQFSILIEAACKYEKPVRIGVNWGSLDPELLARMMDENARSGDPRDASAVM
CCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHH
YEALITSALQSAERAEEIGLGRDRIILSCKMSGVRDLITVYRALAARCDYALHLGLTEAG
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCEEEEECCHHCC
MGSKGIVASTAALSVLLLEGIGDTIRISLTPEPGGDRAREVVVAQEILQTTGLRAFVPLV
CCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHH
AACPGCGRTTSTYFQELAESIQGYVREQMLVWREEYEGVENMTLAVMGCVVNGPGESKHA
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEEEEEEEECCCCCCCCC
NIGISLPGSGERPVAPVFVDGQKAVTLKGDNIAGEFRQIVDEYVQMKYPKKAVDAH
EEEEECCCCCCCCCCEEEECCCEEEEEECCCCHHHHHHHHHHHHHCCCCCHHCCCC
>Mature Secondary Structure 
SMVQSAFPPRRNSVGVQVGSIRIGGGAPIVVQSMTNTDTEDEIATTVQVAQLARAGSEL
CCCHHCCCCCCCCCCEEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCEE
VRITVNTAEAARAVPGIRARLDDMGCQVPLIGDFHFNGHKLVTEYPGCARALAKYRINPG
EEEEEECHHHHHHCCCHHHHHHHCCCCEEEEEEEEECCCEEEECCCHHHHHHHHHCCCCC
NVGHGKKRDEQFSILIEAACKYEKPVRIGVNWGSLDPELLARMMDENARSGDPRDASAVM
CCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHH
YEALITSALQSAERAEEIGLGRDRIILSCKMSGVRDLITVYRALAARCDYALHLGLTEAG
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCEEEEECCHHCC
MGSKGIVASTAALSVLLLEGIGDTIRISLTPEPGGDRAREVVVAQEILQTTGLRAFVPLV
CCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHH
AACPGCGRTTSTYFQELAESIQGYVREQMLVWREEYEGVENMTLAVMGCVVNGPGESKHA
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEEEEEEEECCCCCCCCC
NIGISLPGSGERPVAPVFVDGQKAVTLKGDNIAGEFRQIVDEYVQMKYPKKAVDAH
EEEEECCCCCCCCCCEEEECCCEEEEEECCCCHHHHHHHHHHHHHCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]