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The map label for this gene is hisS [H]

Identifier: 82703491

GI number: 82703491

Start: 2702469

End: 2703728

Strand: Reverse

Name: hisS [H]

Synonym: Nmul_A2376

Alternate gene names: 82703491

Gene position: 2703728-2702469 (Counterclockwise)

Preceding gene: 82703492

Following gene: 82703490

Centisome position: 84.91

GC content: 59.29

Gene sequence:

>1260_bases
ATGCCCACTAGGGGGATTCAGGCTGTACGCGGGATGAACGACATCCTGCCCGATCAGATTGACCGGTGGGAGTTCTTCGA
GCAAAGTGTCCGCGACTGGATGGCGGCTTATGGCTACCGCAATATCCGTATGCCGATAGTCGAGCAGACCGACCTGTTTG
TGCGTTCCATCGGTGCGGTCACAGATATCGTCGAGAAAGAGATGTACACCTTCGTGGATCATCTCAACGGCGAGAGCCTG
ACACTGCGGCCGGAAGGAACCGCGTCCTGCGTGCGTGCGGTGCTTGAGCATAATCTGCTCTATTCCGGCCCGCAACGGCT
ATATTATTCGGGTGCGATGTTCCGCCACGAGCGTCCGCAAAAAGGACGTTACCGGCAATTCCATCAGGTCGGCGCCGAAG
CTCTGGGGTATGGCGGACCCGACATCGATGCCGAGCTCATCATCATGGGCGCCGACTTGTGGAAGCGGCTCGGCGTTTCC
GGGGTGCGGCTCGAAATCGGGACGCTTGGCAGTGCGGAGTCGCGCTCGGTGCACCGTACCCGCCTGATCGATTACCTGCA
GCGGCATCTATGCAAGCTGGATGAAGATGCATCCAGGCGCCTGCACAGCAATCCCCTACGCATACTCGACAGCAAGAATG
CGGGGATGAGAGAGATTATCGAGGGCGCTCCGCGGTTACTGGATGACCTGGACGAGGACTCTCTCATTCATTTTGAACGC
TTGCAGCAAATCCTGCGCGAGCAGGGGGTCGACTTCGAGATCAACCCGCGGCTGGTACGGGGGCTGGATTATTATAATCG
CACCGTATTCGAGTGGGTTACCGACAAGCTGGGGGCGCAGGGAACCGTCTGCGCAGGTGGACGTTATGACGGACTGGTAG
AACAGGTTGGCGGCAAGGCTACCCCCGCATGCGGATTTGCCCTGGGCGTGGAACGAGTGCTGGCACTGGTGATGGACAGT
ATCATCCCTCAGGCTCCTCCTGATGTCTATGTGGTTCACAAGGGCGATGCCGCGGCCGGGTTTGCCTGGAAAACGGCAAG
ACACTTGCGGGATCGTGGGTTCCAGGCAATTCTGCATTGCGGAGAGGGCAGCTTCAAGGCGCAGATGAGAAAAGCCGACG
CCAGCGGAGCGCGTTTTGCGATCATCATCGGAGATGATGAAGCGCAAGCCGGCGAAATAAGCATCAAGCCGCTGCGGGAA
GCGGCGGAGCAGGTCCGGGTAGGCCTTGCGGAAGCTGCCGACCTGCTGAAAAGGGCCTGA

Upstream 100 bases:

>100_bases
GGCCAGAAGGCTGTAACGCTGAAGGGCGATAATATTGCAGGAGAGTTTCGCCAGATAGTCGATGAATATGTGCAGATGAA
ATACCCCAAGAAAGCAGTCG

Downstream 100 bases:

>100_bases
ATCAGAAGAATTCAATTCAACTCGGGAAGATAAATGGCAGCATATAATCCGGAAGAACAGGAAAATATAGACCAGTTGAA
ATCGTGGTGGAACACGTACG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MPTRGIQAVRGMNDILPDQIDRWEFFEQSVRDWMAAYGYRNIRMPIVEQTDLFVRSIGAVTDIVEKEMYTFVDHLNGESL
TLRPEGTASCVRAVLEHNLLYSGPQRLYYSGAMFRHERPQKGRYRQFHQVGAEALGYGGPDIDAELIIMGADLWKRLGVS
GVRLEIGTLGSAESRSVHRTRLIDYLQRHLCKLDEDASRRLHSNPLRILDSKNAGMREIIEGAPRLLDDLDEDSLIHFER
LQQILREQGVDFEINPRLVRGLDYYNRTVFEWVTDKLGAQGTVCAGGRYDGLVEQVGGKATPACGFALGVERVLALVMDS
IIPQAPPDVYVVHKGDAAAGFAWKTARHLRDRGFQAILHCGEGSFKAQMRKADASGARFAIIIGDDEAQAGEISIKPLRE
AAEQVRVGLAEAADLLKRA

Sequences:

>Translated_419_residues
MPTRGIQAVRGMNDILPDQIDRWEFFEQSVRDWMAAYGYRNIRMPIVEQTDLFVRSIGAVTDIVEKEMYTFVDHLNGESL
TLRPEGTASCVRAVLEHNLLYSGPQRLYYSGAMFRHERPQKGRYRQFHQVGAEALGYGGPDIDAELIIMGADLWKRLGVS
GVRLEIGTLGSAESRSVHRTRLIDYLQRHLCKLDEDASRRLHSNPLRILDSKNAGMREIIEGAPRLLDDLDEDSLIHFER
LQQILREQGVDFEINPRLVRGLDYYNRTVFEWVTDKLGAQGTVCAGGRYDGLVEQVGGKATPACGFALGVERVLALVMDS
IIPQAPPDVYVVHKGDAAAGFAWKTARHLRDRGFQAILHCGEGSFKAQMRKADASGARFAIIIGDDEAQAGEISIKPLRE
AAEQVRVGLAEAADLLKRA
>Mature_418_residues
PTRGIQAVRGMNDILPDQIDRWEFFEQSVRDWMAAYGYRNIRMPIVEQTDLFVRSIGAVTDIVEKEMYTFVDHLNGESLT
LRPEGTASCVRAVLEHNLLYSGPQRLYYSGAMFRHERPQKGRYRQFHQVGAEALGYGGPDIDAELIIMGADLWKRLGVSG
VRLEIGTLGSAESRSVHRTRLIDYLQRHLCKLDEDASRRLHSNPLRILDSKNAGMREIIEGAPRLLDDLDEDSLIHFERL
QQILREQGVDFEINPRLVRGLDYYNRTVFEWVTDKLGAQGTVCAGGRYDGLVEQVGGKATPACGFALGVERVLALVMDSI
IPQAPPDVYVVHKGDAAAGFAWKTARHLRDRGFQAILHCGEGSFKAQMRKADASGARFAIIIGDDEAQAGEISIKPLREA
AEQVRVGLAEAADLLKRA

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=474, Percent_Identity=23.8396624472574, Blast_Score=76, Evalue=6e-14,
Organism=Escherichia coli, GI1788861, Length=411, Percent_Identity=54.5012165450122, Blast_Score=436, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 46818; Mature: 46687

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTRGIQAVRGMNDILPDQIDRWEFFEQSVRDWMAAYGYRNIRMPIVEQTDLFVRSIGAV
CCCCHHHHHHCHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
TDIVEKEMYTFVDHLNGESLTLRPEGTASCVRAVLEHNLLYSGPQRLYYSGAMFRHERPQ
HHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHCCCCHHHEECCHHHHCCCCC
KGRYRQFHQVGAEALGYGGPDIDAELIIMGADLWKRLGVSGVRLEIGTLGSAESRSVHRT
CCHHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHCCCCEEEEEECCCCCCCHHHHHH
RLIDYLQRHLCKLDEDASRRLHSNPLRILDSKNAGMREIIEGAPRLLDDLDEDSLIHFER
HHHHHHHHHHHCCCCHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHH
LQQILREQGVDFEINPRLVRGLDYYNRTVFEWVTDKLGAQGTVCAGGRYDGLVEQVGGKA
HHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHCCCC
TPACGFALGVERVLALVMDSIIPQAPPDVYVVHKGDAAAGFAWKTARHLRDRGFQAILHC
CCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCHHHHHCC
GEGSFKAQMRKADASGARFAIIIGDDEAQAGEISIKPLREAAEQVRVGLAEAADLLKRA
CCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PTRGIQAVRGMNDILPDQIDRWEFFEQSVRDWMAAYGYRNIRMPIVEQTDLFVRSIGAV
CCCHHHHHHCHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
TDIVEKEMYTFVDHLNGESLTLRPEGTASCVRAVLEHNLLYSGPQRLYYSGAMFRHERPQ
HHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHCCCCHHHEECCHHHHCCCCC
KGRYRQFHQVGAEALGYGGPDIDAELIIMGADLWKRLGVSGVRLEIGTLGSAESRSVHRT
CCHHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHCCCCEEEEEECCCCCCCHHHHHH
RLIDYLQRHLCKLDEDASRRLHSNPLRILDSKNAGMREIIEGAPRLLDDLDEDSLIHFER
HHHHHHHHHHHCCCCHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHH
LQQILREQGVDFEINPRLVRGLDYYNRTVFEWVTDKLGAQGTVCAGGRYDGLVEQVGGKA
HHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHCCCC
TPACGFALGVERVLALVMDSIIPQAPPDVYVVHKGDAAAGFAWKTARHLRDRGFQAILHC
CCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCHHHHHCC
GEGSFKAQMRKADASGARFAIIIGDDEAQAGEISIKPLREAAEQVRVGLAEAADLLKRA
CCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]