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The map label for this gene is tig

Identifier: 82703458

GI number: 82703458

Start: 2667687

End: 2668994

Strand: Reverse

Name: tig

Synonym: Nmul_A2341

Alternate gene names: 82703458

Gene position: 2668994-2667687 (Counterclockwise)

Preceding gene: 82703459

Following gene: 82703457

Centisome position: 83.82

GC content: 54.82

Gene sequence:

>1308_bases
ATGCAGACAAATGTTGAAAACCTTGGTGCGCTCGAGCGCCGCCTTAACGTTTCAGTTCCCCAGGAGAAAATCGAAACGGA
AGTCGAGAGCCGCTTGAAGCGACTGGCGCGAACAGCAAAATTTCACGGATTTCGGCCGGGCAAGGTACCCTTGAAAATTG
TCGCACAGCAATATGGTCCGCAGGTTCGCCAGGAAGTCATGGAAGACGTGCTGAAAAAGAATTTCAGCGAGGCCGTGCGC
GAGAATAACCTGCGGGTAGCGGGCTATCCGCGCTTCGAACCCAAGCTGGCAGAAAGCGATACTGCTCAGGTTGAATTCAG
TGCGACCTTCGAAGTCTATCCGGATATCACGCTTGGGGATCTTGGCGGCGCGCATATTGAACGTCCGGTAGTCGAGGTTA
CTCCTGCCGATGTGGATAAAACCCTCGAGGTGTTGCGCAAGCAGCGGGTTGAGTTTGAACCTGTGGACCGTCCGGCACAG
GCGGGCGACCGAATCAATATCGACTATCGTGGCTTGATTGACGGTGCCGAGTTTGCGGGTGGCAAAGCTGAAAATTTCAG
TCTGGTGCTGGGCGAAGGAAGGCTTCTCAAGGATTTCGAAGAACCGCTGCTCGGCATGAGTCCGGGTCAGAGCAAAACAT
TTGAAGTTGCCTTTCCATCAGACTATCATGGAAAGGAGGTCGCAGGGAAAACGGCCGTATTTGAAGTGAAGCTCAGCGGC
GTGGAATCTTCGAAACTGCCGGAAGTGAATGCTGATTTTGCCACTTCCCTCGGGATTGCCGAGGGCGATGTTGAAAAGAT
GCGCAGCGAAATCCGGGCGAATCTTGAACGCGAGGCGGCCAAACGCGTAAACGCGAGGCTCAAGGAGCAGGTAATGCAAG
TCCTTATCGATACGACCAGTAACATTGATCCTCCCAAAGCGCTGGTGGAAATGGAACTGGACCGCCTGATGGAGGATGCG
CGCAATGATTTTGCTTCACGCGGCCTGGATACAAAGAATTTCTCCCTGCCGCACGATATGCTCCAGGAGCGGGCGCAACA
TCGGGTGAAGCTGGGCCTGATTCTGGGGGAACTGGTAAAAATGCACAACTTGCACCCCAAACCGGAGCAGGTGCGTGCAG
TGGTGGAAGATCTCGCGCAAGCCTACGAGAATCCGGCCGAAGTAGTCAGTTGGCACTATGCGGCGCCTGAGCGGCTGAGG
GAGGCTGAGTCGGCAGCGCTGGAGGATAACGTGGTGACATGGGTGCTGGAGAAGGCTGTCGTTACTGGCAAGCCGATGCC
GCTGGATGAATTGATGGGAAGATCATAA

Upstream 100 bases:

>100_bases
CACCAGGGATGTGAACAGGTCTGAGCAATGCGTGATGAACGCTAGCGGGTTTCCCTGGCCCCTTCATGATAAATAATATT
GTCAATCCTCAGGAAATTTA

Downstream 100 bases:

>100_bases
ACATGCAACGATTCGAGTGGAAGCAGCGTAATCACGAACCGCAGGATTTGGGTTTGATCCCGATGGTGATTGAAACCAGC
GGGCGGGGCGAGCGCGCATA

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MQTNVENLGALERRLNVSVPQEKIETEVESRLKRLARTAKFHGFRPGKVPLKIVAQQYGPQVRQEVMEDVLKKNFSEAVR
ENNLRVAGYPRFEPKLAESDTAQVEFSATFEVYPDITLGDLGGAHIERPVVEVTPADVDKTLEVLRKQRVEFEPVDRPAQ
AGDRINIDYRGLIDGAEFAGGKAENFSLVLGEGRLLKDFEEPLLGMSPGQSKTFEVAFPSDYHGKEVAGKTAVFEVKLSG
VESSKLPEVNADFATSLGIAEGDVEKMRSEIRANLEREAAKRVNARLKEQVMQVLIDTTSNIDPPKALVEMELDRLMEDA
RNDFASRGLDTKNFSLPHDMLQERAQHRVKLGLILGELVKMHNLHPKPEQVRAVVEDLAQAYENPAEVVSWHYAAPERLR
EAESAALEDNVVTWVLEKAVVTGKPMPLDELMGRS

Sequences:

>Translated_435_residues
MQTNVENLGALERRLNVSVPQEKIETEVESRLKRLARTAKFHGFRPGKVPLKIVAQQYGPQVRQEVMEDVLKKNFSEAVR
ENNLRVAGYPRFEPKLAESDTAQVEFSATFEVYPDITLGDLGGAHIERPVVEVTPADVDKTLEVLRKQRVEFEPVDRPAQ
AGDRINIDYRGLIDGAEFAGGKAENFSLVLGEGRLLKDFEEPLLGMSPGQSKTFEVAFPSDYHGKEVAGKTAVFEVKLSG
VESSKLPEVNADFATSLGIAEGDVEKMRSEIRANLEREAAKRVNARLKEQVMQVLIDTTSNIDPPKALVEMELDRLMEDA
RNDFASRGLDTKNFSLPHDMLQERAQHRVKLGLILGELVKMHNLHPKPEQVRAVVEDLAQAYENPAEVVSWHYAAPERLR
EAESAALEDNVVTWVLEKAVVTGKPMPLDELMGRS
>Mature_435_residues
MQTNVENLGALERRLNVSVPQEKIETEVESRLKRLARTAKFHGFRPGKVPLKIVAQQYGPQVRQEVMEDVLKKNFSEAVR
ENNLRVAGYPRFEPKLAESDTAQVEFSATFEVYPDITLGDLGGAHIERPVVEVTPADVDKTLEVLRKQRVEFEPVDRPAQ
AGDRINIDYRGLIDGAEFAGGKAENFSLVLGEGRLLKDFEEPLLGMSPGQSKTFEVAFPSDYHGKEVAGKTAVFEVKLSG
VESSKLPEVNADFATSLGIAEGDVEKMRSEIRANLEREAAKRVNARLKEQVMQVLIDTTSNIDPPKALVEMELDRLMEDA
RNDFASRGLDTKNFSLPHDMLQERAQHRVKLGLILGELVKMHNLHPKPEQVRAVVEDLAQAYENPAEVVSWHYAAPERLR
EAESAALEDNVVTWVLEKAVVTGKPMPLDELMGRS

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=437, Percent_Identity=39.1304347826087, Blast_Score=327, Evalue=1e-90,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_NITMU (Q2Y6I9)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_413024.1
- HSSP:   Q9RT21
- ProteinModelPortal:   Q2Y6I9
- SMR:   Q2Y6I9
- STRING:   Q2Y6I9
- GeneID:   3784744
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A2341
- eggNOG:   COG0544
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- PhylomeDB:   Q2Y6I9
- ProtClustDB:   PRK01490
- BioCyc:   NMUL323848:NMUL_A2341-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PANTHER:   PTHR10516
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 48598; Mature: 48598

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTNVENLGALERRLNVSVPQEKIETEVESRLKRLARTAKFHGFRPGKVPLKIVAQQYGP
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCH
QVRQEVMEDVLKKNFSEAVRENNLRVAGYPRFEPKLAESDTAQVEFSATFEVYPDITLGD
HHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCEEEEEEEEEECCCCEECC
LGGAHIERPVVEVTPADVDKTLEVLRKQRVEFEPVDRPAQAGDRINIDYRGLIDGAEFAG
CCCCHHCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHCCCEECCEECCCCCCHHHCC
GKAENFSLVLGEGRLLKDFEEPLLGMSPGQSKTFEVAFPSDYHGKEVAGKTAVFEVKLSG
CCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHCCCEEEEEEEECC
VESSKLPEVNADFATSLGIAEGDVEKMRSEIRANLEREAAKRVNARLKEQVMQVLIDTTS
CCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NIDPPKALVEMELDRLMEDARNDFASRGLDTKNFSLPHDMLQERAQHRVKLGLILGELVK
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
MHNLHPKPEQVRAVVEDLAQAYENPAEVVSWHYAAPERLREAESAALEDNVVTWVLEKAV
HHCCCCCHHHHHHHHHHHHHHHCCHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VTGKPMPLDELMGRS
HCCCCCCHHHHCCCC
>Mature Secondary Structure
MQTNVENLGALERRLNVSVPQEKIETEVESRLKRLARTAKFHGFRPGKVPLKIVAQQYGP
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCH
QVRQEVMEDVLKKNFSEAVRENNLRVAGYPRFEPKLAESDTAQVEFSATFEVYPDITLGD
HHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCEEEEEEEEEECCCCEECC
LGGAHIERPVVEVTPADVDKTLEVLRKQRVEFEPVDRPAQAGDRINIDYRGLIDGAEFAG
CCCCHHCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHCCCEECCEECCCCCCHHHCC
GKAENFSLVLGEGRLLKDFEEPLLGMSPGQSKTFEVAFPSDYHGKEVAGKTAVFEVKLSG
CCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHCCCEEEEEEEECC
VESSKLPEVNADFATSLGIAEGDVEKMRSEIRANLEREAAKRVNARLKEQVMQVLIDTTS
CCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NIDPPKALVEMELDRLMEDARNDFASRGLDTKNFSLPHDMLQERAQHRVKLGLILGELVK
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
MHNLHPKPEQVRAVVEDLAQAYENPAEVVSWHYAAPERLREAESAALEDNVVTWVLEKAV
HHCCCCCHHHHHHHHHHHHHHHCCHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VTGKPMPLDELMGRS
HCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA