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The map label for this gene is glnA [H]

Identifier: 82703406

GI number: 82703406

Start: 2603604

End: 2605013

Strand: Direct

Name: glnA [H]

Synonym: Nmul_A2288

Alternate gene names: 82703406

Gene position: 2603604-2605013 (Clockwise)

Preceding gene: 82703404

Following gene: 82703407

Centisome position: 81.77

GC content: 54.47

Gene sequence:

>1410_bases
ATGGCGGTAGCCGATGTTCTGAAAATGATTCAGGACAACGAAGTCAGATTCGTTGATCTACGATTTACCGATACCCGCGG
TAAAGAACAGCATGTAACCCTGCCCGCTCACGCCGTCGATGCGGACAAACTGGAGGACGGTCATACATTCGATGGCTCTT
CCATCGCCGGCTGGAAGGGCGTGCAGGCGTCGGATATGCTCCTGATACCGGATACCGGTACTGCCCGGATGGATCCGTTC
ATGGACGAGGCCACCTTGTTCATCACCTGCGATGTGGTGGAGCCTGCGGATGGGAAGGGTTACGACCGGGATCCCCGTTC
CCTGGCAAAACGCGGCGAAGCCTACCTCAAGTCGACCGGGATCGGCGATACCGCGTTTTTTGGTCCGGAGCCTGAATTCT
TCATTTTTGATTCCGTAACCTGGCACGCCGACATGTCCGGCTGTTCCGTAAAAATCGAGTCGGAAGAAGCAGCCTGGAGT
TCGGGAGTAAAATATGAGGGTGGCAATATCGGGCATCGTCCTGGTATCAAAGGGGGCTATTCCCCGGTTCCCCCGATCGA
TTCCTTGCACGACATCCGGTCGGCGATGTGTCTTGCACTTGAGGAAATGGGTATCGAAGTTGAAGTGCACCACCACGAAA
CGGCGACTGCCGGCCAGTGTGAAATAGGCACCAAGTACAGCAGCCTGGTGAAGCGTGCCGACTGGACGCAGGTATTCAAA
TACGTGGTGCACAACATTGCCCGCTCGTATGGAAAAACCGCCACCTTCATGCCAAAACCCATTGTAGGTGACAATGGCTC
CGGGATGCATGTGCACCAGTCGATCTGGAAAGGCGGACGAAACCTGTTTTCCGGAAACGGGTATGCCGGGCTTTCGGAAA
TGGCAATGTATTATATTGGCGGAATCATCAACCATGCCAAGGCATTGAATGCCATTACCAACCCGGGGACCAACTCGTAC
AAGCGCCTCGTTCCAGGCTTTGAAGCACCCATCAATCTTGCATATTCCGTAAGTAACCGTTCGGCCGCTATCCGCATTCC
TTATGCCAACAACCCCAAAGGCCGCCGGATCGAGGTGCGCTTCCCCGACCCTACCGCGAACCCTTACCTTGCATTTACTG
CCATGCTGATGGCGGGGCTGGATGGAATCCAGAACAAGAGTCACCCGGGCGATCCGATGGACAAGAATCTCTACGACCTT
CCACCCGAGGAGGCTGCCAGAATTCCCAATCCTTGCGCTTCCCTGGATGAAGCACTGGACAGCCTGGATAAGGATCGTAA
TTTCCTCACTCGCGGCGGGGTGTTTTCGAATGATATGATAGATGCCTACATCGAACTCAAGATGGAAGAAGTTACGCGCT
TCCGGATGACGACGCACCCGGTCGAATTCGATATGTACTACAGCTTGTGA

Upstream 100 bases:

>100_bases
TAATATCAATTCTGCAAATTCCCTTTATATGGGGACACTCCGCTTTTTAAAAGGAAGGCGCTTTCGCACGCTTGCATATT
GATATGGTTTGGAGACTGAA

Downstream 100 bases:

>100_bases
GCACTGTTTCTCACGCTCGGACCGCTCATGCTGTTTCATTGATCAGGACATAATATCCGATGAAAATTCTTTTTGTTACA
TTGCTCGCGCTGCCAATGCT

Product: glutamine synthetase, type I

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MAVADVLKMIQDNEVRFVDLRFTDTRGKEQHVTLPAHAVDADKLEDGHTFDGSSIAGWKGVQASDMLLIPDTGTARMDPF
MDEATLFITCDVVEPADGKGYDRDPRSLAKRGEAYLKSTGIGDTAFFGPEPEFFIFDSVTWHADMSGCSVKIESEEAAWS
SGVKYEGGNIGHRPGIKGGYSPVPPIDSLHDIRSAMCLALEEMGIEVEVHHHETATAGQCEIGTKYSSLVKRADWTQVFK
YVVHNIARSYGKTATFMPKPIVGDNGSGMHVHQSIWKGGRNLFSGNGYAGLSEMAMYYIGGIINHAKALNAITNPGTNSY
KRLVPGFEAPINLAYSVSNRSAAIRIPYANNPKGRRIEVRFPDPTANPYLAFTAMLMAGLDGIQNKSHPGDPMDKNLYDL
PPEEAARIPNPCASLDEALDSLDKDRNFLTRGGVFSNDMIDAYIELKMEEVTRFRMTTHPVEFDMYYSL

Sequences:

>Translated_469_residues
MAVADVLKMIQDNEVRFVDLRFTDTRGKEQHVTLPAHAVDADKLEDGHTFDGSSIAGWKGVQASDMLLIPDTGTARMDPF
MDEATLFITCDVVEPADGKGYDRDPRSLAKRGEAYLKSTGIGDTAFFGPEPEFFIFDSVTWHADMSGCSVKIESEEAAWS
SGVKYEGGNIGHRPGIKGGYSPVPPIDSLHDIRSAMCLALEEMGIEVEVHHHETATAGQCEIGTKYSSLVKRADWTQVFK
YVVHNIARSYGKTATFMPKPIVGDNGSGMHVHQSIWKGGRNLFSGNGYAGLSEMAMYYIGGIINHAKALNAITNPGTNSY
KRLVPGFEAPINLAYSVSNRSAAIRIPYANNPKGRRIEVRFPDPTANPYLAFTAMLMAGLDGIQNKSHPGDPMDKNLYDL
PPEEAARIPNPCASLDEALDSLDKDRNFLTRGGVFSNDMIDAYIELKMEEVTRFRMTTHPVEFDMYYSL
>Mature_468_residues
AVADVLKMIQDNEVRFVDLRFTDTRGKEQHVTLPAHAVDADKLEDGHTFDGSSIAGWKGVQASDMLLIPDTGTARMDPFM
DEATLFITCDVVEPADGKGYDRDPRSLAKRGEAYLKSTGIGDTAFFGPEPEFFIFDSVTWHADMSGCSVKIESEEAAWSS
GVKYEGGNIGHRPGIKGGYSPVPPIDSLHDIRSAMCLALEEMGIEVEVHHHETATAGQCEIGTKYSSLVKRADWTQVFKY
VVHNIARSYGKTATFMPKPIVGDNGSGMHVHQSIWKGGRNLFSGNGYAGLSEMAMYYIGGIINHAKALNAITNPGTNSYK
RLVPGFEAPINLAYSVSNRSAAIRIPYANNPKGRRIEVRFPDPTANPYLAFTAMLMAGLDGIQNKSHPGDPMDKNLYDLP
PEEAARIPNPCASLDEALDSLDKDRNFLTRGGVFSNDMIDAYIELKMEEVTRFRMTTHPVEFDMYYSL

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=443, Percent_Identity=23.9277652370203, Blast_Score=79, Evalue=9e-15,
Organism=Escherichia coli, GI1790301, Length=469, Percent_Identity=64.3923240938166, Blast_Score=665, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=393, Percent_Identity=25.4452926208651, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 51626; Mature: 51495

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVADVLKMIQDNEVRFVDLRFTDTRGKEQHVTLPAHAVDADKLEDGHTFDGSSIAGWKG
CCHHHHHHHHCCCCEEEEEEEEECCCCCCCEEECCHHHCCHHHCCCCCCCCCCCCCCCCC
VQASDMLLIPDTGTARMDPFMDEATLFITCDVVEPADGKGYDRDPRSLAKRGEAYLKSTG
CCCCCEEEECCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCC
IGDTAFFGPEPEFFIFDSVTWHADMSGCSVKIESEEAAWSSGVKYEGGNIGHRPGIKGGY
CCCCCCCCCCCCEEEEECEEEEECCCCCEEEECCCHHHHHCCCEECCCCCCCCCCCCCCC
SPVPPIDSLHDIRSAMCLALEEMGIEVEVHHHETATAGQCEIGTKYSSLVKRADWTQVFK
CCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHCCHHHHHH
YVVHNIARSYGKTATFMPKPIVGDNGSGMHVHQSIWKGGRNLFSGNGYAGLSEMAMYYIG
HHHHHHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
GIINHAKALNAITNPGTNSYKRLVPGFEAPINLAYSVSNRSAAIRIPYANNPKGRRIEVR
HHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCEEEEE
FPDPTANPYLAFTAMLMAGLDGIQNKSHPGDPMDKNLYDLPPEEAARIPNPCASLDEALD
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHH
SLDKDRNFLTRGGVFSNDMIDAYIELKMEEVTRFRMTTHPVEFDMYYSL
HHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECC
>Mature Secondary Structure 
AVADVLKMIQDNEVRFVDLRFTDTRGKEQHVTLPAHAVDADKLEDGHTFDGSSIAGWKG
CHHHHHHHHCCCCEEEEEEEEECCCCCCCEEECCHHHCCHHHCCCCCCCCCCCCCCCCC
VQASDMLLIPDTGTARMDPFMDEATLFITCDVVEPADGKGYDRDPRSLAKRGEAYLKSTG
CCCCCEEEECCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCC
IGDTAFFGPEPEFFIFDSVTWHADMSGCSVKIESEEAAWSSGVKYEGGNIGHRPGIKGGY
CCCCCCCCCCCCEEEEECEEEEECCCCCEEEECCCHHHHHCCCEECCCCCCCCCCCCCCC
SPVPPIDSLHDIRSAMCLALEEMGIEVEVHHHETATAGQCEIGTKYSSLVKRADWTQVFK
CCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHCCHHHHHH
YVVHNIARSYGKTATFMPKPIVGDNGSGMHVHQSIWKGGRNLFSGNGYAGLSEMAMYYIG
HHHHHHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
GIINHAKALNAITNPGTNSYKRLVPGFEAPINLAYSVSNRSAAIRIPYANNPKGRRIEVR
HHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCEEEEE
FPDPTANPYLAFTAMLMAGLDGIQNKSHPGDPMDKNLYDLPPEEAARIPNPCASLDEALD
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHH
SLDKDRNFLTRGGVFSNDMIDAYIELKMEEVTRFRMTTHPVEFDMYYSL
HHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1969923 [H]