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The map label for this gene is thiC

Identifier: 82703403

GI number: 82703403

Start: 2599705

End: 2601612

Strand: Direct

Name: thiC

Synonym: Nmul_A2285

Alternate gene names: 82703403

Gene position: 2599705-2601612 (Clockwise)

Preceding gene: 82703398

Following gene: 82703404

Centisome position: 81.64

GC content: 54.82

Gene sequence:

>1908_bases
ATGAATGCAACAGTTTCAAGCGCGGTCCAAAGTTCGCTGCCTTTTTCGGGCAAGACTGCGCAAGTTGACGAAGGCACGGT
CAAACCTCTGCCCCGGTCACAAAAAACCTACCTGAGCGGTTCCCGCCCGGATATCCGGGTTCCCATGCGTGAAATCAGCC
AGTCCGATACGCCCGCCAGCATGGGAGCGGAAAAAAATCCGCCCATTTATGTCTATGATACTTCCGGTCCTTATACCGAC
CCGGCAATCAAAATTGACATTCGCGCCGGCTTGGCGCCGCTGCGCGAAAAGTGGATAGATGAGCGCGGAGATACGGAGAT
CCTCTCGGGTCCTGCCTCCATCTATGGCAGGCAACGTCTGAACGATCCGCGTCTCGCCGAACTGCGCTTTGACTTGAAAC
GCAGTCCCCGCCGCGCCAGAGCTGGAGCAAACGTGACACAAATGCATTATGCCCGAGGCGGCATCGTCACCCCGGAAATG
GAATTCATTGCCATACGGGAGAATCAGCGGTGCGAGCATTTGGCCGATCAACAGCGGGAAATGCTTGCGCGCCAACACCC
GGGCCAGGATTTCGGCGCGTTTCTGCCGCGCCACATCACGCCGGAGTTCGTACGCGACGAGGTCGCCAGGGGACGCGCAA
TCATTCCCGCCAACATCAATCATCCCGAATCCGAACCCATGATCATCGGGCGCAACTTTCTGGTGAAAATCAACGCAAAT
ATCGGTAATTCGGCACTAAGCTCAAGTATCCAGGAAGAAGTGGAAAAGATGACATGGGCGATACGCTGGGGAGGGGATAC
CGTAATGGATCTCTCCACGGGAAAAAACATTCATGAAACGCGCGAATGGATCATACGCAACAGTCCCGTTCCCATCGGCA
CGGTGCCGATCTACCAGGCCCTGGAAAAAGTAAATGGCAAGGCCGAAGATCTGACCTGGGAAATTTTTCGCGATACCCTG
ATAGAGCAGGCTGAACAGGGGGTGGACTATTTCACCATTCATGCCGGCGTACGGCTCGCCTATGTTCCGATGACCGCAAA
ACGGCTCACCGGTATCGTTTCCCGCGGCGGATCGATCATGGCGAAGTGGTGCCTTGCCCACCACAAAGAGAGTTTCCTGT
ATACGCAATTCGAGGAAATCTGCGAAATCATGAAGGCTTACGATGTGAGCTTCTCCCTCGGCGACGGATTGCGGCCCGGT
TCAATATACGATGCGAATGATGAAGCGCAGTTTGCGGAGCTGAAAACCCTCGGTGAACTGACGCAGATTGCCTGGAAGCA
TGATGTGCAGGTGATGATCGAAGGCCCCGGCCATGTTCCCATGCATCTCATCAAGGAGAACATGGATATGCAGCTGAAAT
ACTGCGCCGAAGCCCCGTTCTATACGTTGGGGCCGCTCACTACCGACATCGCTCCCGGGTACGATCATATTACCTCTGCC
ATCGGCGCTGCCATGATCGGCTGGTACGGTACCGCGATGTTATGTTATGTGACCCCCAAGGAGCATCTCGGCCTGCCGGA
CAAGGATGACGTCAAGGATGGCATCATCACCTATAAAATCGCTGCCCATGCCGCAGACCTGGCAAAAGGACACCCCGGCG
CCCAATTACGCGACAATGCTCTATCCAAAGCGCGCTTCGAGTTTCGCTGGGAAGATCAGTTCAACCTTGGCCTCGATCCC
GACAAGGCAAGGCAATTCCATGATGAAACTCTGCCGCAGGAAGGCGCGAAGCTCGCCCATTTCTGTTCGATGTGCGGTCC
GCATTTCTGCTCAATGAAAATCACACAGGATGTACGCGACTTTGCGGCAAGCAAAGGTGTCAGCGACCAAGAGGCCCTGG
AAAAAGGCATGGAAGAAAAAGCGAGTGAATTTGTAGCAAGGGGAACCGAGATTTACAGCAAGGTGTAA

Upstream 100 bases:

>100_bases
CGATGGTTCCTCTACCTGACACGGATAATGCCGCCGTAGGGAAGCTGGGAATCGGGTTGTCCGGTCGTCCCGCTCCTTTA
TGGCACTCAAGGAGCGTAAT

Downstream 100 bases:

>100_bases
GCGACATGACAGCCGGCTTGTGCCGGCTTGCCGTTGAAAAAGCAAAAGAGGAAGGCGGCAGGAGAATGCGGGCGAGATTC
CATTGCCAGGACCTTCTCCT

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 635; Mature: 635

Protein sequence:

>635_residues
MNATVSSAVQSSLPFSGKTAQVDEGTVKPLPRSQKTYLSGSRPDIRVPMREISQSDTPASMGAEKNPPIYVYDTSGPYTD
PAIKIDIRAGLAPLREKWIDERGDTEILSGPASIYGRQRLNDPRLAELRFDLKRSPRRARAGANVTQMHYARGGIVTPEM
EFIAIRENQRCEHLADQQREMLARQHPGQDFGAFLPRHITPEFVRDEVARGRAIIPANINHPESEPMIIGRNFLVKINAN
IGNSALSSSIQEEVEKMTWAIRWGGDTVMDLSTGKNIHETREWIIRNSPVPIGTVPIYQALEKVNGKAEDLTWEIFRDTL
IEQAEQGVDYFTIHAGVRLAYVPMTAKRLTGIVSRGGSIMAKWCLAHHKESFLYTQFEEICEIMKAYDVSFSLGDGLRPG
SIYDANDEAQFAELKTLGELTQIAWKHDVQVMIEGPGHVPMHLIKENMDMQLKYCAEAPFYTLGPLTTDIAPGYDHITSA
IGAAMIGWYGTAMLCYVTPKEHLGLPDKDDVKDGIITYKIAAHAADLAKGHPGAQLRDNALSKARFEFRWEDQFNLGLDP
DKARQFHDETLPQEGAKLAHFCSMCGPHFCSMKITQDVRDFAASKGVSDQEALEKGMEEKASEFVARGTEIYSKV

Sequences:

>Translated_635_residues
MNATVSSAVQSSLPFSGKTAQVDEGTVKPLPRSQKTYLSGSRPDIRVPMREISQSDTPASMGAEKNPPIYVYDTSGPYTD
PAIKIDIRAGLAPLREKWIDERGDTEILSGPASIYGRQRLNDPRLAELRFDLKRSPRRARAGANVTQMHYARGGIVTPEM
EFIAIRENQRCEHLADQQREMLARQHPGQDFGAFLPRHITPEFVRDEVARGRAIIPANINHPESEPMIIGRNFLVKINAN
IGNSALSSSIQEEVEKMTWAIRWGGDTVMDLSTGKNIHETREWIIRNSPVPIGTVPIYQALEKVNGKAEDLTWEIFRDTL
IEQAEQGVDYFTIHAGVRLAYVPMTAKRLTGIVSRGGSIMAKWCLAHHKESFLYTQFEEICEIMKAYDVSFSLGDGLRPG
SIYDANDEAQFAELKTLGELTQIAWKHDVQVMIEGPGHVPMHLIKENMDMQLKYCAEAPFYTLGPLTTDIAPGYDHITSA
IGAAMIGWYGTAMLCYVTPKEHLGLPDKDDVKDGIITYKIAAHAADLAKGHPGAQLRDNALSKARFEFRWEDQFNLGLDP
DKARQFHDETLPQEGAKLAHFCSMCGPHFCSMKITQDVRDFAASKGVSDQEALEKGMEEKASEFVARGTEIYSKV
>Mature_635_residues
MNATVSSAVQSSLPFSGKTAQVDEGTVKPLPRSQKTYLSGSRPDIRVPMREISQSDTPASMGAEKNPPIYVYDTSGPYTD
PAIKIDIRAGLAPLREKWIDERGDTEILSGPASIYGRQRLNDPRLAELRFDLKRSPRRARAGANVTQMHYARGGIVTPEM
EFIAIRENQRCEHLADQQREMLARQHPGQDFGAFLPRHITPEFVRDEVARGRAIIPANINHPESEPMIIGRNFLVKINAN
IGNSALSSSIQEEVEKMTWAIRWGGDTVMDLSTGKNIHETREWIIRNSPVPIGTVPIYQALEKVNGKAEDLTWEIFRDTL
IEQAEQGVDYFTIHAGVRLAYVPMTAKRLTGIVSRGGSIMAKWCLAHHKESFLYTQFEEICEIMKAYDVSFSLGDGLRPG
SIYDANDEAQFAELKTLGELTQIAWKHDVQVMIEGPGHVPMHLIKENMDMQLKYCAEAPFYTLGPLTTDIAPGYDHITSA
IGAAMIGWYGTAMLCYVTPKEHLGLPDKDDVKDGIITYKIAAHAADLAKGHPGAQLRDNALSKARFEFRWEDQFNLGLDP
DKARQFHDETLPQEGAKLAHFCSMCGPHFCSMKITQDVRDFAASKGVSDQEALEKGMEEKASEFVARGTEIYSKV

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=618, Percent_Identity=72.0064724919094, Blast_Score=921, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_NITMU (Q2Y6P4)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_412969.1
- STRING:   Q2Y6P4
- GeneID:   3785101
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A2285
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   TWELFRD
- PhylomeDB:   Q2Y6P4
- ProtClustDB:   PRK09284
- BioCyc:   NMUL323848:NMUL_A2285-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 70809; Mature: 70809

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: BINDING 240-240 BINDING 269-269 BINDING 298-298 BINDING 334-334 BINDING 434-434 BINDING 461-461

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNATVSSAVQSSLPFSGKTAQVDEGTVKPLPRSQKTYLSGSRPDIRVPMREISQSDTPAS
CCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCEECCHHHCCCCCCCHH
MGAEKNPPIYVYDTSGPYTDPAIKIDIRAGLAPLREKWIDERGDTEILSGPASIYGRQRL
CCCCCCCCEEEEECCCCCCCCEEEEEECCCCHHHHHHHCCCCCCCHHHCCCHHHHHHHCC
NDPRLAELRFDLKRSPRRARAGANVTQMHYARGGIVTPEMEFIAIRENQRCEHLADQQRE
CCCCHHHHHHHHHCCCHHHHCCCCHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHH
MLARQHPGQDFGAFLPRHITPEFVRDEVARGRAIIPANINHPESEPMIIGRNFLVKINAN
HHHHHCCCCCHHHHCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEECCEEEEEEECC
IGNSALSSSIQEEVEKMTWAIRWGGDTVMDLSTGKNIHETREWIIRNSPVPIGTVPIYQA
CCCHHHHHHHHHHHHHHEEEEEECCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHH
LEKVNGKAEDLTWEIFRDTLIEQAEQGVDYFTIHAGVRLAYVPMTAKRLTGIVSRGGSIM
HHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEECCHHHHHHHHHHCCCHHH
AKWCLAHHKESFLYTQFEEICEIMKAYDVSFSLGDGLRPGSIYDANDEAQFAELKTLGEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCEECCCCCHHHHHHHHHHHH
TQIAWKHDVQVMIEGPGHVPMHLIKENMDMQLKYCAEAPFYTLGPLTTDIAPGYDHITSA
HHHHHHCCCEEEEECCCCCCHHHHHCCCCCEEEECCCCCCEEECCCCCCCCCCHHHHHHH
IGAAMIGWYGTAMLCYVTPKEHLGLPDKDDVKDGIITYKIAAHAADLAKGHPGAQLRDNA
HHHHHHHHHHHEEEEEECCHHHCCCCCCCCCCCCEEEEEHHHHHHHHHCCCCCCCHHHHH
LSKARFEFRWEDQFNLGLDPDKARQFHDETLPQEGAKLAHFCSMCGPHFCSMKITQDVRD
HHHCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHH
FAASKGVSDQEALEKGMEEKASEFVARGTEIYSKV
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNATVSSAVQSSLPFSGKTAQVDEGTVKPLPRSQKTYLSGSRPDIRVPMREISQSDTPAS
CCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCEECCHHHCCCCCCCHH
MGAEKNPPIYVYDTSGPYTDPAIKIDIRAGLAPLREKWIDERGDTEILSGPASIYGRQRL
CCCCCCCCEEEEECCCCCCCCEEEEEECCCCHHHHHHHCCCCCCCHHHCCCHHHHHHHCC
NDPRLAELRFDLKRSPRRARAGANVTQMHYARGGIVTPEMEFIAIRENQRCEHLADQQRE
CCCCHHHHHHHHHCCCHHHHCCCCHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHH
MLARQHPGQDFGAFLPRHITPEFVRDEVARGRAIIPANINHPESEPMIIGRNFLVKINAN
HHHHHCCCCCHHHHCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEECCEEEEEEECC
IGNSALSSSIQEEVEKMTWAIRWGGDTVMDLSTGKNIHETREWIIRNSPVPIGTVPIYQA
CCCHHHHHHHHHHHHHHEEEEEECCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHH
LEKVNGKAEDLTWEIFRDTLIEQAEQGVDYFTIHAGVRLAYVPMTAKRLTGIVSRGGSIM
HHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEECCHHHHHHHHHHCCCHHH
AKWCLAHHKESFLYTQFEEICEIMKAYDVSFSLGDGLRPGSIYDANDEAQFAELKTLGEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCEECCCCCHHHHHHHHHHHH
TQIAWKHDVQVMIEGPGHVPMHLIKENMDMQLKYCAEAPFYTLGPLTTDIAPGYDHITSA
HHHHHHCCCEEEEECCCCCCHHHHHCCCCCEEEECCCCCCEEECCCCCCCCCCHHHHHHH
IGAAMIGWYGTAMLCYVTPKEHLGLPDKDDVKDGIITYKIAAHAADLAKGHPGAQLRDNA
HHHHHHHHHHHEEEEEECCHHHCCCCCCCCCCCCEEEEEHHHHHHHHHCCCCCCCHHHHH
LSKARFEFRWEDQFNLGLDPDKARQFHDETLPQEGAKLAHFCSMCGPHFCSMKITQDVRD
HHHCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHH
FAASKGVSDQEALEKGMEEKASEFVARGTEIYSKV
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA