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The map label for this gene is metG

Identifier: 82703057

GI number: 82703057

Start: 2225075

End: 2227261

Strand: Reverse

Name: metG

Synonym: Nmul_A1936

Alternate gene names: 82703057

Gene position: 2227261-2225075 (Counterclockwise)

Preceding gene: 82703058

Following gene: 82703050

Centisome position: 69.95

GC content: 52.45

Gene sequence:

>2187_bases
ATGAACAAACGAAAAATCCTCGTCACCTCGGCCCTCCCTTATGCCAACGGCAGCATCCATCTCGGCCATCTGGTGGAGTA
CATCCAGACTGACATCTGGGTTCGTTTCCAGAAAATGCAAGAGCACGAGGTCCATTATGTTTGCGCCGACGACGCACATG
GCACGCCGATCATGCTTCGCGCAGAACAGGAAGGCATCACTCCGCGACAGCTGATAGACCGCGTATGGCATGAGCATAAG
ACGGATTTCGATGGTTTCCATATTGGCTTCGATCACTATTACACTACCGATTCGCCCGAGAATCAGGCGTTTTGCGAAGA
TATCTACCGTCAGCTCAGGGCAGGGGAGCTGATTGCCAAACGTTCGGTGGAACAGTTTTATGATCCGGTCAAGCAGATGT
TTCTGCCGGATCGGTACATCAAGGGCGAATGCCCCAGATGTCATGCCGGAGACCAGTATGGAGATTCCTGTGAGGCTTGC
GGAGCCACCTATCTGCCAACCGACCTGATCAGTCCCTACTCTGCCGTCTCCGGCGCCCGACCGGAGCGCCGCACTTCGAA
TCACTATTTCTTCAAACTTTCGGATGTGCGTTGCGAGGCTTTCCTCAAAAACTGGATTTTTGAATCCATCCCGGCTCAGC
CCGGCGTCGCCTTGGAAACGAGACCGCGACTTCAACCTGAAGCTGCCAATAAAATGAACGAGTGGCTTTCAGCGGGTCTC
GTGGACTGGGATATCTCGCGGGATGCGCCCTATTTCGGCTTCCCTATTCCACGTACCGGCGGAAAGAAATTTTTCTATGT
CTGGCTGGATGCGCCGGTCGGTTATTTCGGCAGTTTCAAGAATTACTTCAAGCAGAACCAGCGAACCCAGGCCGAGATCG
ATGAATTCCTCCGTCCTGGTGGCAATACGGAAATGGTGCATTTCATAGGGAAGGATATTCTCTACTTTCACGCCCTGTTC
TGGCCGGCGATGCTGGAATTCTCGGGATATCGCACCCCTACTCAGATTTATGCTCACGGGTTTCTAACCGTCAACGGCCA
GAAAATGTCCAAATCCAGGGGGACTTTCATCACTGCTGAGAGCTATCTGAAGCAAGGACTGAATCCGGAATGGTTGCGTT
ATTACTACGCTGCCAAACTCAACGACAGCATGGAGGATATCGATCTCAGCTTCGAGGATTTTGTCGCGCGCGTGAACAGC
GATCTGGTAGGGAAATATGTCAATATCGCCAGCAGATGCGCAGGGTTCATCACCAAAAAATTCGGGGGCGAGCTTACACA
CAATGTTTCGGAAGCGAGCCGGAAATGGTTCAATCAGTTTCTTTTTTGCGAACTGGGAGAGGGAGATACTTTTCTCGGCC
GTCATATGTCCATCGCAAATTTCTATGAACGCAGGGAGTACAGTAAAGCGATCAAGGAAATCATGTCGGCGGCCGATGTG
GCCAACCAATATGTGGATCGGATGAAACCCTGGGTACTGGCGAAAGACAGCAGGAACGACAGGGAATTGCATGAAGTATG
CAGCGTCGCGTTGAATATGTTCCGGATGCTGACGGTGTATCTGAAACCTGTGCTGCCGAAGCTTGCAATGGAAGCGGAGC
AGTTCCTGGGACTAGATCCTCTTACATGGAAGGATGCAAACTCGCAGCACAGGTTATTGCCGGACGGACATCGGATCAAT
GATTACCAGCACTTGATGACCCGGATCGATCCGAAGCAGATCGAAATGCTTACCCATGCAAACAAGGAAAGCCTGGCACC
CGCAAAGTCACAGCAGGTTGCACAAGCTGTAGAAACCATGGAAAAAAACTCTTCCACCACACCCGCCCCGGCAAAAGAAG
GAGAAGCAGGCCAAGCCAGCGCGTCGACCATCTCCATCGAGGATTTCGGCAAGATTGATCTCAGGGTCGCGAAAATCCTC
GATGCGGAGCACGTGCCGGGCGCCGACAAGCTGCTCAAGCTAACCCTGGATGTCGCTTCGGAACAGCGAACAGTATTTGC
AGGCATAAAATCAGCTTATGATCCCGGACAGCTCAAGGGACGCCTCACGGTCATGGTTGCCAACCTCGCACCGCGCAAGA
TGAAATTCGGTATATCCGAAGGAATGGTACTGGCTGCGGGAGACGGTGAAGGCCCTTATCTGCTTTCTCCCGATGAAGGT
GCGCGCCCCGGAATGAAGGTGAAATAA

Upstream 100 bases:

>100_bases
GGGTCTGGAAATCCTTTTCGTCAGCCCCAAAGACCGCAAGCCCCACAGTTTGTTACAATCATCGTTTTGCGTCATTCCGA
ACCCCTTTCTCAATCGCTTG

Downstream 100 bases:

>100_bases
CGAGAATGAATTCCTGAACCTGACGAACCTGGCGAAGTGTGACCGGAATTGCAGCTCAAGGCTTTTCCGCCTCGAACAGT
ATGGCCTGCATTGGATAACG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 728; Mature: 728

Protein sequence:

>728_residues
MNKRKILVTSALPYANGSIHLGHLVEYIQTDIWVRFQKMQEHEVHYVCADDAHGTPIMLRAEQEGITPRQLIDRVWHEHK
TDFDGFHIGFDHYYTTDSPENQAFCEDIYRQLRAGELIAKRSVEQFYDPVKQMFLPDRYIKGECPRCHAGDQYGDSCEAC
GATYLPTDLISPYSAVSGARPERRTSNHYFFKLSDVRCEAFLKNWIFESIPAQPGVALETRPRLQPEAANKMNEWLSAGL
VDWDISRDAPYFGFPIPRTGGKKFFYVWLDAPVGYFGSFKNYFKQNQRTQAEIDEFLRPGGNTEMVHFIGKDILYFHALF
WPAMLEFSGYRTPTQIYAHGFLTVNGQKMSKSRGTFITAESYLKQGLNPEWLRYYYAAKLNDSMEDIDLSFEDFVARVNS
DLVGKYVNIASRCAGFITKKFGGELTHNVSEASRKWFNQFLFCELGEGDTFLGRHMSIANFYERREYSKAIKEIMSAADV
ANQYVDRMKPWVLAKDSRNDRELHEVCSVALNMFRMLTVYLKPVLPKLAMEAEQFLGLDPLTWKDANSQHRLLPDGHRIN
DYQHLMTRIDPKQIEMLTHANKESLAPAKSQQVAQAVETMEKNSSTTPAPAKEGEAGQASASTISIEDFGKIDLRVAKIL
DAEHVPGADKLLKLTLDVASEQRTVFAGIKSAYDPGQLKGRLTVMVANLAPRKMKFGISEGMVLAAGDGEGPYLLSPDEG
ARPGMKVK

Sequences:

>Translated_728_residues
MNKRKILVTSALPYANGSIHLGHLVEYIQTDIWVRFQKMQEHEVHYVCADDAHGTPIMLRAEQEGITPRQLIDRVWHEHK
TDFDGFHIGFDHYYTTDSPENQAFCEDIYRQLRAGELIAKRSVEQFYDPVKQMFLPDRYIKGECPRCHAGDQYGDSCEAC
GATYLPTDLISPYSAVSGARPERRTSNHYFFKLSDVRCEAFLKNWIFESIPAQPGVALETRPRLQPEAANKMNEWLSAGL
VDWDISRDAPYFGFPIPRTGGKKFFYVWLDAPVGYFGSFKNYFKQNQRTQAEIDEFLRPGGNTEMVHFIGKDILYFHALF
WPAMLEFSGYRTPTQIYAHGFLTVNGQKMSKSRGTFITAESYLKQGLNPEWLRYYYAAKLNDSMEDIDLSFEDFVARVNS
DLVGKYVNIASRCAGFITKKFGGELTHNVSEASRKWFNQFLFCELGEGDTFLGRHMSIANFYERREYSKAIKEIMSAADV
ANQYVDRMKPWVLAKDSRNDRELHEVCSVALNMFRMLTVYLKPVLPKLAMEAEQFLGLDPLTWKDANSQHRLLPDGHRIN
DYQHLMTRIDPKQIEMLTHANKESLAPAKSQQVAQAVETMEKNSSTTPAPAKEGEAGQASASTISIEDFGKIDLRVAKIL
DAEHVPGADKLLKLTLDVASEQRTVFAGIKSAYDPGQLKGRLTVMVANLAPRKMKFGISEGMVLAAGDGEGPYLLSPDEG
ARPGMKVK
>Mature_728_residues
MNKRKILVTSALPYANGSIHLGHLVEYIQTDIWVRFQKMQEHEVHYVCADDAHGTPIMLRAEQEGITPRQLIDRVWHEHK
TDFDGFHIGFDHYYTTDSPENQAFCEDIYRQLRAGELIAKRSVEQFYDPVKQMFLPDRYIKGECPRCHAGDQYGDSCEAC
GATYLPTDLISPYSAVSGARPERRTSNHYFFKLSDVRCEAFLKNWIFESIPAQPGVALETRPRLQPEAANKMNEWLSAGL
VDWDISRDAPYFGFPIPRTGGKKFFYVWLDAPVGYFGSFKNYFKQNQRTQAEIDEFLRPGGNTEMVHFIGKDILYFHALF
WPAMLEFSGYRTPTQIYAHGFLTVNGQKMSKSRGTFITAESYLKQGLNPEWLRYYYAAKLNDSMEDIDLSFEDFVARVNS
DLVGKYVNIASRCAGFITKKFGGELTHNVSEASRKWFNQFLFCELGEGDTFLGRHMSIANFYERREYSKAIKEIMSAADV
ANQYVDRMKPWVLAKDSRNDRELHEVCSVALNMFRMLTVYLKPVLPKLAMEAEQFLGLDPLTWKDANSQHRLLPDGHRIN
DYQHLMTRIDPKQIEMLTHANKESLAPAKSQQVAQAVETMEKNSSTTPAPAKEGEAGQASASTISIEDFGKIDLRVAKIL
DAEHVPGADKLLKLTLDVASEQRTVFAGIKSAYDPGQLKGRLTVMVANLAPRKMKFGISEGMVLAAGDGEGPYLLSPDEG
ARPGMKVK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=663, Percent_Identity=26.6968325791855, Blast_Score=239, Evalue=7e-63,
Organism=Homo sapiens, GI24308436, Length=550, Percent_Identity=25.8181818181818, Blast_Score=134, Evalue=3e-31,
Organism=Escherichia coli, GI1788432, Length=728, Percent_Identity=51.6483516483517, Blast_Score=730, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17540794, Length=755, Percent_Identity=27.4172185430464, Blast_Score=251, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI212645096, Length=556, Percent_Identity=22.6618705035971, Blast_Score=119, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI71992781, Length=294, Percent_Identity=25.1700680272109, Blast_Score=89, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6321704, Length=582, Percent_Identity=29.3814432989691, Blast_Score=248, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6321610, Length=563, Percent_Identity=23.2682060390764, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24655311, Length=590, Percent_Identity=27.4576271186441, Blast_Score=222, Evalue=6e-58,
Organism=Drosophila melanogaster, GI21358483, Length=544, Percent_Identity=24.6323529411765, Blast_Score=131, Evalue=1e-30,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_NITMU (Q2Y7P0)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_412623.1
- ProteinModelPortal:   Q2Y7P0
- STRING:   Q2Y7P0
- GeneID:   3784232
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A1936
- eggNOG:   COG0143
- HOGENOM:   HBG721667
- OMA:   CEAVPES
- PhylomeDB:   Q2Y7P0
- BioCyc:   NMUL323848:NMUL_A1936-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 82634; Mature: 82634

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 351-351

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKRKILVTSALPYANGSIHLGHLVEYIQTDIWVRFQKMQEHEVHYVCADDAHGTPIMLR
CCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEE
AEQEGITPRQLIDRVWHEHKTDFDGFHIGFDHYYTTDSPENQAFCEDIYRQLRAGELIAK
ECCCCCCHHHHHHHHHHHCCCCCCCEEECCCCEECCCCCCCHHHHHHHHHHHHHHHHHHH
RSVEQFYDPVKQMFLPDRYIKGECPRCHAGDQYGDSCEACGATYLPTDLISPYSAVSGAR
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCHHHCCCHHHHCCCC
PERRTSNHYFFKLSDVRCEAFLKNWIFESIPAQPGVALETRPRLQPEAANKMNEWLSAGL
CCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCHHHHHHHHHHHCCC
VDWDISRDAPYFGFPIPRTGGKKFFYVWLDAPVGYFGSFKNYFKQNQRTQAEIDEFLRPG
EEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHCCC
GNTEMVHFIGKDILYFHALFWPAMLEFSGYRTPTQIYAHGFLTVNGQKMSKSRGTFITAE
CCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCHHHCCCCCEEEEH
SYLKQGLNPEWLRYYYAAKLNDSMEDIDLSFEDFVARVNSDLVGKYVNIASRCAGFITKK
HHHHCCCCHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
FGGELTHNVSEASRKWFNQFLFCELGEGDTFLGRHMSIANFYERREYSKAIKEIMSAADV
HCCHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANQYVDRMKPWVLAKDSRNDRELHEVCSVALNMFRMLTVYLKPVLPKLAMEAEQFLGLDP
HHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LTWKDANSQHRLLPDGHRINDYQHLMTRIDPKQIEMLTHANKESLAPAKSQQVAQAVETM
CCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHCCHHHCCCCHHHHHHHHHHHH
EKNSSTTPAPAKEGEAGQASASTISIEDFGKIDLRVAKILDAEHVPGADKLLKLTLDVAS
HHCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCC
EQRTVFAGIKSAYDPGQLKGRLTVMVANLAPRKMKFGISEGMVLAAGDGEGPYLLSPDEG
CCHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHCCCCCCCEEEEECCCCCCEEECCCCC
ARPGMKVK
CCCCCCCC
>Mature Secondary Structure
MNKRKILVTSALPYANGSIHLGHLVEYIQTDIWVRFQKMQEHEVHYVCADDAHGTPIMLR
CCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEE
AEQEGITPRQLIDRVWHEHKTDFDGFHIGFDHYYTTDSPENQAFCEDIYRQLRAGELIAK
ECCCCCCHHHHHHHHHHHCCCCCCCEEECCCCEECCCCCCCHHHHHHHHHHHHHHHHHHH
RSVEQFYDPVKQMFLPDRYIKGECPRCHAGDQYGDSCEACGATYLPTDLISPYSAVSGAR
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCHHHCCCHHHHCCCC
PERRTSNHYFFKLSDVRCEAFLKNWIFESIPAQPGVALETRPRLQPEAANKMNEWLSAGL
CCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCHHHHHHHHHHHCCC
VDWDISRDAPYFGFPIPRTGGKKFFYVWLDAPVGYFGSFKNYFKQNQRTQAEIDEFLRPG
EEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHCCC
GNTEMVHFIGKDILYFHALFWPAMLEFSGYRTPTQIYAHGFLTVNGQKMSKSRGTFITAE
CCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCHHHCCCCCEEEEH
SYLKQGLNPEWLRYYYAAKLNDSMEDIDLSFEDFVARVNSDLVGKYVNIASRCAGFITKK
HHHHCCCCHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
FGGELTHNVSEASRKWFNQFLFCELGEGDTFLGRHMSIANFYERREYSKAIKEIMSAADV
HCCHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANQYVDRMKPWVLAKDSRNDRELHEVCSVALNMFRMLTVYLKPVLPKLAMEAEQFLGLDP
HHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LTWKDANSQHRLLPDGHRINDYQHLMTRIDPKQIEMLTHANKESLAPAKSQQVAQAVETM
CCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHCCHHHCCCCHHHHHHHHHHHH
EKNSSTTPAPAKEGEAGQASASTISIEDFGKIDLRVAKILDAEHVPGADKLLKLTLDVAS
HHCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCC
EQRTVFAGIKSAYDPGQLKGRLTVMVANLAPRKMKFGISEGMVLAAGDGEGPYLLSPDEG
CCHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHCCCCCCCEEEEECCCCCCEEECCCCC
ARPGMKVK
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA