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The map label for this gene is leuC [H]

Identifier: 82703042

GI number: 82703042

Start: 2209401

End: 2210810

Strand: Reverse

Name: leuC [H]

Synonym: Nmul_A1921

Alternate gene names: 82703042

Gene position: 2210810-2209401 (Counterclockwise)

Preceding gene: 82703043

Following gene: 82703041

Centisome position: 69.43

GC content: 58.65

Gene sequence:

>1410_bases
ATGCAAACGTTATACGACAAACTCTGGCAAAGCCATGTGGTGCATGAAGAATCCGATGATGCGGAAGGCATGGCGCTGCT
ATATATCGATCGTCACCTCGTGCACGAGGTGACCAGCCCCCAAGCGTTCGAAGGGTTGAAGCTGGCCGGACGCAAGCCGT
GGCGCCTCAATTCCATTCTGGCGGTAGCAGACCACAACGTGCCGACCACCGGGCGAGATCATGGCATCAGTGATCCGGTA
TCTCGCCTGCAAGTGGAAACGCTCGATCAGAATTGTGAGGAATTGGGCATCACCGAATTCAGAATGAACGATCAGCGCCA
AGGCATCGTGCATGTCATCGGACCGGAACAGGGTGCCACCCTGCCAGGAATGACAGTGGTTTGCGGCGACTCGCATACAA
GCACGCATGGCGCTTTTGGCTGCCTTGCCTTTGGCATAGGTACTTCCGAGGTGGAGCATGTGCTGGCGACGCAGTGCTTG
CTGGCGAAGAAATCCAGAACGATGCAGATCGTGGTAGATGGCAACCTGGGCAATGGCATTACCGCCAAGGATGTGGCGCT
TGCCGTGGTCGGCAGGATCGGTACTGCCGGAGGTACAGGCTATGCGATCGAGTTTGCCGGCAGCGCGATTCGTGGGCTGT
CGATGGAAGGGCGCATGACGCTCTGCAATATGGCGATCGAGGCGGGTGCGCGTGCGGGAATGGTGGCAGTGGATGATGTC
ACCATTGAATATCTCCGGGGCCGTCCCTTTGCGCCTAAAGGCGATCTCTGGGAAAAAGCGGTGGCCTACTGGCGTACCCT
GAAGAGTGACGAGGGTGCGAGCTTTGACAAGGGGGTTCAACTGGACGCCGCCTCGATCAAGCCGCAGGTAACCTGGGGAA
CTTCCCCCGAAATGGTTGCGACAGTGGATGGAAAGGTGCCTGATCCGACAGAAATCGCGGATGCGGTGAAGCGTCACGAC
ATGGAGCGGGCGCTCAAATACATGGCGCTGGCTCCCAACACCCCGATCAGTGAAATTCGCCCCGATAAAATATTCATCGG
TTCCTGCACGAATGCGCGCATAGAGGATTTGCGCGCCGCCGCCGAGGTGGTGAGGGGCCGTCGCATTGCGAAGAGCATCA
AGCTTGCAATGGTCGTACCAGGATCGGGCCTGGTCAAGCATCAGGCAGAGCAGGAGGGGCTGGACAGGATATTCCGCGAT
GCGGGCTTTGAATGGCGCGAACCGGGTTGCTCGATGTGCCTGGCGATGAATGACGACAGGCTGGAGCCCGGCGAACGGTG
CGCTTCCACTTCCAATCGCAACTTTGAGGGCAGGCAGGGTCCCGCGGGGCGTACTCACCTGGTGAGCCCTGCGATGGCCG
CCGCTGCCGCAGTCGCGGGACATTTTGTGGATGTAAGGGAAATATATTAG

Upstream 100 bases:

>100_bases
GGTCCGCGTGACAATTGCGTACTCTTGCTGTTCCTTGATATTTCATTATGGGATAATGAAACATTGAGATTTGAAGCCGT
TATTCCCATTTTCCACAAAA

Downstream 100 bases:

>100_bases
AATTCCAGTACAACAATAACGGTTTTTTATACTACTACAGGGAGTTGTGATGCGAACGCTGACGATGATGCTTGCCTTGA
TCGCTGCCTTTGGCCTGTCT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MQTLYDKLWQSHVVHEESDDAEGMALLYIDRHLVHEVTSPQAFEGLKLAGRKPWRLNSILAVADHNVPTTGRDHGISDPV
SRLQVETLDQNCEELGITEFRMNDQRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGCLAFGIGTSEVEHVLATQCL
LAKKSRTMQIVVDGNLGNGITAKDVALAVVGRIGTAGGTGYAIEFAGSAIRGLSMEGRMTLCNMAIEAGARAGMVAVDDV
TIEYLRGRPFAPKGDLWEKAVAYWRTLKSDEGASFDKGVQLDAASIKPQVTWGTSPEMVATVDGKVPDPTEIADAVKRHD
MERALKYMALAPNTPISEIRPDKIFIGSCTNARIEDLRAAAEVVRGRRIAKSIKLAMVVPGSGLVKHQAEQEGLDRIFRD
AGFEWREPGCSMCLAMNDDRLEPGERCASTSNRNFEGRQGPAGRTHLVSPAMAAAAAVAGHFVDVREIY

Sequences:

>Translated_469_residues
MQTLYDKLWQSHVVHEESDDAEGMALLYIDRHLVHEVTSPQAFEGLKLAGRKPWRLNSILAVADHNVPTTGRDHGISDPV
SRLQVETLDQNCEELGITEFRMNDQRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGCLAFGIGTSEVEHVLATQCL
LAKKSRTMQIVVDGNLGNGITAKDVALAVVGRIGTAGGTGYAIEFAGSAIRGLSMEGRMTLCNMAIEAGARAGMVAVDDV
TIEYLRGRPFAPKGDLWEKAVAYWRTLKSDEGASFDKGVQLDAASIKPQVTWGTSPEMVATVDGKVPDPTEIADAVKRHD
MERALKYMALAPNTPISEIRPDKIFIGSCTNARIEDLRAAAEVVRGRRIAKSIKLAMVVPGSGLVKHQAEQEGLDRIFRD
AGFEWREPGCSMCLAMNDDRLEPGERCASTSNRNFEGRQGPAGRTHLVSPAMAAAAAVAGHFVDVREIY
>Mature_469_residues
MQTLYDKLWQSHVVHEESDDAEGMALLYIDRHLVHEVTSPQAFEGLKLAGRKPWRLNSILAVADHNVPTTGRDHGISDPV
SRLQVETLDQNCEELGITEFRMNDQRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGCLAFGIGTSEVEHVLATQCL
LAKKSRTMQIVVDGNLGNGITAKDVALAVVGRIGTAGGTGYAIEFAGSAIRGLSMEGRMTLCNMAIEAGARAGMVAVDDV
TIEYLRGRPFAPKGDLWEKAVAYWRTLKSDEGASFDKGVQLDAASIKPQVTWGTSPEMVATVDGKVPDPTEIADAVKRHD
MERALKYMALAPNTPISEIRPDKIFIGSCTNARIEDLRAAAEVVRGRRIAKSIKLAMVVPGSGLVKHQAEQEGLDRIFRD
AGFEWREPGCSMCLAMNDDRLEPGERCASTSNRNFEGRQGPAGRTHLVSPAMAAAAAVAGHFVDVREIY

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=361, Percent_Identity=26.3157894736842, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI8659555, Length=477, Percent_Identity=24.5283018867925, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI41352693, Length=380, Percent_Identity=27.1052631578947, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1786259, Length=467, Percent_Identity=62.5267665952891, Blast_Score=598, Evalue=1e-172,
Organism=Escherichia coli, GI1787531, Length=362, Percent_Identity=27.0718232044199, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI87081781, Length=360, Percent_Identity=23.0555555555556, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25149337, Length=363, Percent_Identity=27.8236914600551, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32564738, Length=363, Percent_Identity=27.8236914600551, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI25149342, Length=305, Percent_Identity=26.5573770491803, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17568399, Length=393, Percent_Identity=25.6997455470738, Blast_Score=105, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6321429, Length=473, Percent_Identity=64.9048625792812, Blast_Score=625, Evalue=1e-180,
Organism=Saccharomyces cerevisiae, GI6323335, Length=361, Percent_Identity=28.5318559556787, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6320440, Length=390, Percent_Identity=26.6666666666667, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6322261, Length=365, Percent_Identity=26.3013698630137, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI281365315, Length=474, Percent_Identity=23.2067510548523, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI17864292, Length=474, Percent_Identity=23.2067510548523, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI28571643, Length=474, Percent_Identity=24.0506329113924, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI161076999, Length=363, Percent_Identity=24.5179063360882, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI17137564, Length=388, Percent_Identity=26.8041237113402, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24645686, Length=385, Percent_Identity=27.5324675324675, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50654; Mature: 50654

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTLYDKLWQSHVVHEESDDAEGMALLYIDRHLVHEVTSPQAFEGLKLAGRKPWRLNSIL
CCHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHCCCCHHHCCHHHCCCCCCCCCCEE
AVADHNVPTTGRDHGISDPVSRLQVETLDQNCEELGITEFRMNDQRQGIVHVIGPEQGAT
EEECCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHCCCEEEECCCCCCCEEEEECCCCCCC
LPGMTVVCGDSHTSTHGAFGCLAFGIGTSEVEHVLATQCLLAKKSRTMQIVVDGNLGNGI
CCCEEEEECCCCCCCCCCHHHHEECCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCC
TAKDVALAVVGRIGTAGGTGYAIEFAGSAIRGLSMEGRMTLCNMAIEAGARAGMVAVDDV
CHHHHHHHHHHHCCCCCCCCEEEEECCCHHHCCCCCCCHHHHHHHHHCCCCCCEEEECHH
TIEYLRGRPFAPKGDLWEKAVAYWRTLKSDEGASFDKGVQLDAASIKPQVTWGTSPEMVA
HHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCEEEECCCCCEEE
TVDGKVPDPTEIADAVKRHDMERALKYMALAPNTPISEIRPDKIFIGSCTNARIEDLRAA
EECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCEEEEECCCCCHHHHHHHH
AEVVRGRRIAKSIKLAMVVPGSGLVKHQAEQEGLDRIFRDAGFEWREPGCSMCLAMNDDR
HHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCC
LEPGERCASTSNRNFEGRQGPAGRTHLVSPAMAAAAAVAGHFVDVREIY
CCCHHHHHCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQTLYDKLWQSHVVHEESDDAEGMALLYIDRHLVHEVTSPQAFEGLKLAGRKPWRLNSIL
CCHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHCCCCHHHCCHHHCCCCCCCCCCEE
AVADHNVPTTGRDHGISDPVSRLQVETLDQNCEELGITEFRMNDQRQGIVHVIGPEQGAT
EEECCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHCCCEEEECCCCCCCEEEEECCCCCCC
LPGMTVVCGDSHTSTHGAFGCLAFGIGTSEVEHVLATQCLLAKKSRTMQIVVDGNLGNGI
CCCEEEEECCCCCCCCCCHHHHEECCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCC
TAKDVALAVVGRIGTAGGTGYAIEFAGSAIRGLSMEGRMTLCNMAIEAGARAGMVAVDDV
CHHHHHHHHHHHCCCCCCCCEEEEECCCHHHCCCCCCCHHHHHHHHHCCCCCCEEEECHH
TIEYLRGRPFAPKGDLWEKAVAYWRTLKSDEGASFDKGVQLDAASIKPQVTWGTSPEMVA
HHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCEEEECCCCCEEE
TVDGKVPDPTEIADAVKRHDMERALKYMALAPNTPISEIRPDKIFIGSCTNARIEDLRAA
EECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCEEEEECCCCCHHHHHHHH
AEVVRGRRIAKSIKLAMVVPGSGLVKHQAEQEGLDRIFRDAGFEWREPGCSMCLAMNDDR
HHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCC
LEPGERCASTSNRNFEGRQGPAGRTHLVSPAMAAAAAVAGHFVDVREIY
CCCHHHHHCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]