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The map label for this gene is truA [H]

Identifier: 82703035

GI number: 82703035

Start: 2204065

End: 2205120

Strand: Reverse

Name: truA [H]

Synonym: Nmul_A1914

Alternate gene names: 82703035

Gene position: 2205120-2204065 (Counterclockwise)

Preceding gene: 82703036

Following gene: 82703034

Centisome position: 69.25

GC content: 55.59

Gene sequence:

>1056_bases
ATGGCTTGTGCGAAAGCGGTGGGCAATGACGGACTACCCAATCAGCGCCCAATGTCACGCTGGAGAGCGTCTGCTTATAC
GTCATTACTTCCGTTTCCCGAAAAGTGTTTAAAAATTCCCTGGACGTGCCGGTACAAGTATCACCAGAATCACGATGGTA
GCTTTACCGTGAGAATCGCAATGGTGCTGGAGTATGACGGCAGCAATTTCTGCGGCTGGCAAAGCCAGCCAGGGGGAAAT
ACCGTGCAGGATGCCGTGGAAGCGGCCTTGTCTGAAATTGCAGGCGAGGCTATCCGAGTAGTGACGGCAGGGAGAACCGA
CGCAGGGGTTCATGCGATCTACCAGGTGCTGCATTTCGATACTCGGGCGGAGCGACCTATGAATGCATGGGTGCGGGGTG
CAAACGCCCTGCTGCCCAGCGGCATTGCCCTGCTATGGGCATCCCCTACTGCAGACGATTTTCATGCCCGCTACTGTGCG
CTTGAGCGTTGTTACCTCTACCTGTTACTGAACCACCCAGTGCGGCCGGGCCTTCATCAGCACCGAGTCGGCTGGTATCA
CCATCCGCTCCGTCTCGAATCCATGCAGATGGGGGCACAAATGCTGGTGGGCGAACACGATTTCAGCGCCTTTCGGGCTG
CTGCATGCCAGGCCAAATCCCCTGTACGCACCCTGACAAAACTGGAAGTTACGCGAGTGGGAAACATGGTTGCGTTTGAG
CTGCGCGCTAATGCATTTTTGCACCACATGGTACGGAATATCGTCGGTTGTCTGGTCTATGTAGGTAAAGGTAAATTTCG
TCCTGACTGGATAGGGAAACTGCTTGAAAACGGGAAGCGCAGCGAAGCTGCACCGACTTTTTCCGCTTCCGGGTTATACT
TGGCAGGGGTTGCCTATGATGCGAGGTGGAAGCTGCCACCCTTTGTCGAGCCCCCTCTGACCGCAATAGTGCCGGGCACA
AACAGGCCGGCTATCCTCACATCATGGGCGACAAGTGGCGGAAACCCAGTTGCGGGCGCAACACCGGAAGTCAGGGATAT
ATGTCGATTCGAGTAA

Upstream 100 bases:

>100_bases
CACAAAAAACAGTCTTCTGGAACAGATGAACGGGAAAAAGAAGAGGAACCGCTGCCGTGGGCACTCGTTCTGTAGCGGGA
AAGCATAAGAGTCGTACAGG

Downstream 100 bases:

>100_bases
AAGTTTGCGGTATAACCCGAGTGGAAGATGCGCTTGCGGCAGTGCATCTCGGAGCGAATGCCGTGGGCTTTGTCTTCTGG
GAGCAGAGCGCGCGCTATAT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MACAKAVGNDGLPNQRPMSRWRASAYTSLLPFPEKCLKIPWTCRYKYHQNHDGSFTVRIAMVLEYDGSNFCGWQSQPGGN
TVQDAVEAALSEIAGEAIRVVTAGRTDAGVHAIYQVLHFDTRAERPMNAWVRGANALLPSGIALLWASPTADDFHARYCA
LERCYLYLLLNHPVRPGLHQHRVGWYHHPLRLESMQMGAQMLVGEHDFSAFRAAACQAKSPVRTLTKLEVTRVGNMVAFE
LRANAFLHHMVRNIVGCLVYVGKGKFRPDWIGKLLENGKRSEAAPTFSASGLYLAGVAYDARWKLPPFVEPPLTAIVPGT
NRPAILTSWATSGGNPVAGATPEVRDICRFE

Sequences:

>Translated_351_residues
MACAKAVGNDGLPNQRPMSRWRASAYTSLLPFPEKCLKIPWTCRYKYHQNHDGSFTVRIAMVLEYDGSNFCGWQSQPGGN
TVQDAVEAALSEIAGEAIRVVTAGRTDAGVHAIYQVLHFDTRAERPMNAWVRGANALLPSGIALLWASPTADDFHARYCA
LERCYLYLLLNHPVRPGLHQHRVGWYHHPLRLESMQMGAQMLVGEHDFSAFRAAACQAKSPVRTLTKLEVTRVGNMVAFE
LRANAFLHHMVRNIVGCLVYVGKGKFRPDWIGKLLENGKRSEAAPTFSASGLYLAGVAYDARWKLPPFVEPPLTAIVPGT
NRPAILTSWATSGGNPVAGATPEVRDICRFE
>Mature_350_residues
ACAKAVGNDGLPNQRPMSRWRASAYTSLLPFPEKCLKIPWTCRYKYHQNHDGSFTVRIAMVLEYDGSNFCGWQSQPGGNT
VQDAVEAALSEIAGEAIRVVTAGRTDAGVHAIYQVLHFDTRAERPMNAWVRGANALLPSGIALLWASPTADDFHARYCAL
ERCYLYLLLNHPVRPGLHQHRVGWYHHPLRLESMQMGAQMLVGEHDFSAFRAAACQAKSPVRTLTKLEVTRVGNMVAFEL
RANAFLHHMVRNIVGCLVYVGKGKFRPDWIGKLLENGKRSEAAPTFSASGLYLAGVAYDARWKLPPFVEPPLTAIVPGTN
RPAILTSWATSGGNPVAGATPEVRDICRFE

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=281, Percent_Identity=31.6725978647687, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI199559785, Length=284, Percent_Identity=25.7042253521127, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1788657, Length=256, Percent_Identity=47.265625, Blast_Score=235, Evalue=3e-63,
Organism=Caenorhabditis elegans, GI71985046, Length=323, Percent_Identity=27.5541795665635, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 38784; Mature: 38653

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACAKAVGNDGLPNQRPMSRWRASAYTSLLPFPEKCLKIPWTCRYKYHQNHDGSFTVRIA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHCCCCEEEEEEECCCCCCEEEEEE
MVLEYDGSNFCGWQSQPGGNTVQDAVEAALSEIAGEAIRVVTAGRTDAGVHAIYQVLHFD
EEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHC
TRAERPMNAWVRGANALLPSGIALLWASPTADDFHARYCALERCYLYLLLNHPVRPGLHQ
CCCCCCHHHHHHCCCHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHH
HRVGWYHHPLRLESMQMGAQMLVGEHDFSAFRAAACQAKSPVRTLTKLEVTRVGNMVAFE
CCCCCEECCEEHHHHHHCHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEE
LRANAFLHHMVRNIVGCLVYVGKGKFRPDWIGKLLENGKRSEAAPTFSASGLYLAGVAYD
HHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEC
ARWKLPPFVEPPLTAIVPGTNRPAILTSWATSGGNPVAGATPEVRDICRFE
CCCCCCCCCCCCCEEEECCCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCC
>Mature Secondary Structure 
ACAKAVGNDGLPNQRPMSRWRASAYTSLLPFPEKCLKIPWTCRYKYHQNHDGSFTVRIA
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHCCCCEEEEEEECCCCCCEEEEEE
MVLEYDGSNFCGWQSQPGGNTVQDAVEAALSEIAGEAIRVVTAGRTDAGVHAIYQVLHFD
EEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHC
TRAERPMNAWVRGANALLPSGIALLWASPTADDFHARYCALERCYLYLLLNHPVRPGLHQ
CCCCCCHHHHHHCCCHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHH
HRVGWYHHPLRLESMQMGAQMLVGEHDFSAFRAAACQAKSPVRTLTKLEVTRVGNMVAFE
CCCCCEECCEEHHHHHHCHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEE
LRANAFLHHMVRNIVGCLVYVGKGKFRPDWIGKLLENGKRSEAAPTFSASGLYLAGVAYD
HHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEC
ARWKLPPFVEPPLTAIVPGTNRPAILTSWATSGGNPVAGATPEVRDICRFE
CCCCCCCCCCCCCEEEECCCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]