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The map label for this gene is nusA [H]

Identifier: 82702986

GI number: 82702986

Start: 2151210

End: 2152697

Strand: Reverse

Name: nusA [H]

Synonym: Nmul_A1865

Alternate gene names: 82702986

Gene position: 2152697-2151210 (Counterclockwise)

Preceding gene: 82702987

Following gene: 82702985

Centisome position: 67.6

GC content: 55.85

Gene sequence:

>1488_bases
ATGAGCCGCGAGGTTTTGCTATTGGTGGATGCACTGGCGCGCGAAAAGAATGTGGAAAAGAACATTGTTTTTGCCGCACT
TGAGCTTGCATTGGCGTCCGCCACGAAAAAGCGTTTTAACGAGGACGCTGACGTGCGCGTGTCGATCGACCACCAGACAG
GGGACTATCAATCCTTGCGCCGCTGGCAGGTGGTGGCCGACGATGCAGTGGAGGATCCCGCTCGTCAGATATCGCTGAGC
GAGGCCTTTCGGCAAAATCCTGAAATCCAGCTCGACGAATATATCGAAGAAATCCTGGAGCCGGTGGAATTCGGGCGCAT
CGGCGCCCAAGCGGCGAAACAGGTGATATTCCAGAAAATCCGTGATGCCGAACGCGAGCAGATCCTGAACGATTTTCTCG
AGCGCAAGGAATATATGGTTACCGGAACCATCAAGCGCATGGAACGCGGAAACGCGATCATCGAATCCGGCAGGGTAGAG
GCCGTATTGCCGCGTGACCAGATGATTCCCAAGGAGAATCTGCGTGTCGGGGACAGAGTGCGCGCTTACTTGCACAGGAT
CGACCGTACTACGAGAGGTCCGCAGTTAATCCTGTCGAGAATTGTGCCGGAATTTCTGATAAAGCTGTTTGAGCTGGAAG
TGCCTGAGATCGAGGAGGGCTTGCTGGAGATCAAGGTGGCAGCGCGTGATCCTGGTTCGCGTGCGAAGATCGCAGTGAAG
TCGAATGATCAGCGAGTGGATCCGATCGGTACCTGCGTAGGCATGCGGGGTTCCAGAGTCCAGGCCGTGACCGGCGAACT
GGCGGGTGAAAGGGTAGACATCATCCTTTGGTCTGACGACCCCGCCACATTCGTAATCAATGCGCTGGCTCCTGCCGAGA
TCAGCAGCATTCTGGTGGATGAAGAGAAACACAGCATGGACATTGTCGTGGATGAAGGCAATCTGGCCCAGGCGATCGGA
CGTGGAGGCCAGAATGTCCGGCTCGCCTCGGAATTGACAGGCTGGGAGCTCAACATCATGACGATGGAAGAGTCACAGGC
AAAAAATGAAGAGGAGTTTTCGGTTTTGCGCCATCTCTTCATGGAAAAACTGGATGTGGATGAGGAAGTGGCGGACATTC
TGGCGCAGGAAGGCTTTACTACTCTGGAAGAAGTGGCTTACGTGCCGCTCAGCGAAATGATGGAGATCGAAGCATTTGAC
GAACAGACCGTCAACGAGCTTCGCAATCGGGCTCGCAACGCATTGTTGACCGAGGCCATCGTGAGCGAGGAAAAGGTGGA
GCACATCGCCGAAGACCTGCTCGCGCTGGAAGGCATGGACAGCCAGACCGCGCGCGAACTCGCGGCTAAAGGAGTAAGCA
CCCAGGAAGACCTGGCCGACCTGGCTGTGGATGATCTGGTGGAAATGGTCGAGATGGATACTGAACGGGCCAGGCAGTTG
ATCATGGCGGCACGTGCCCCGTGGTTTGCCCCGGCCGATAGAGCATAG

Upstream 100 bases:

>100_bases
TGGAAGTAGATGGAAAAATGCTGGACCTTGAACTCACTAACCTTGAGAAGGTTCGCCTCGTTCCGAAATTGTAATCTATG
AGTAACTGGCTGGAGGTAAC

Downstream 100 bases:

>100_bases
CCGACTGAAGGTAATTAATGTCTCAAATGAACGTAGAGCAATTTGCTAGTGAACTTGGCGTGCTTCCCACCGTGCTGCTG
GAGCAGTTGCAGGCGGCCGG

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MSREVLLLVDALAREKNVEKNIVFAALELALASATKKRFNEDADVRVSIDHQTGDYQSLRRWQVVADDAVEDPARQISLS
EAFRQNPEIQLDEYIEEILEPVEFGRIGAQAAKQVIFQKIRDAEREQILNDFLERKEYMVTGTIKRMERGNAIIESGRVE
AVLPRDQMIPKENLRVGDRVRAYLHRIDRTTRGPQLILSRIVPEFLIKLFELEVPEIEEGLLEIKVAARDPGSRAKIAVK
SNDQRVDPIGTCVGMRGSRVQAVTGELAGERVDIILWSDDPATFVINALAPAEISSILVDEEKHSMDIVVDEGNLAQAIG
RGGQNVRLASELTGWELNIMTMEESQAKNEEEFSVLRHLFMEKLDVDEEVADILAQEGFTTLEEVAYVPLSEMMEIEAFD
EQTVNELRNRARNALLTEAIVSEEKVEHIAEDLLALEGMDSQTARELAAKGVSTQEDLADLAVDDLVEMVEMDTERARQL
IMAARAPWFAPADRA

Sequences:

>Translated_495_residues
MSREVLLLVDALAREKNVEKNIVFAALELALASATKKRFNEDADVRVSIDHQTGDYQSLRRWQVVADDAVEDPARQISLS
EAFRQNPEIQLDEYIEEILEPVEFGRIGAQAAKQVIFQKIRDAEREQILNDFLERKEYMVTGTIKRMERGNAIIESGRVE
AVLPRDQMIPKENLRVGDRVRAYLHRIDRTTRGPQLILSRIVPEFLIKLFELEVPEIEEGLLEIKVAARDPGSRAKIAVK
SNDQRVDPIGTCVGMRGSRVQAVTGELAGERVDIILWSDDPATFVINALAPAEISSILVDEEKHSMDIVVDEGNLAQAIG
RGGQNVRLASELTGWELNIMTMEESQAKNEEEFSVLRHLFMEKLDVDEEVADILAQEGFTTLEEVAYVPLSEMMEIEAFD
EQTVNELRNRARNALLTEAIVSEEKVEHIAEDLLALEGMDSQTARELAAKGVSTQEDLADLAVDDLVEMVEMDTERARQL
IMAARAPWFAPADRA
>Mature_494_residues
SREVLLLVDALAREKNVEKNIVFAALELALASATKKRFNEDADVRVSIDHQTGDYQSLRRWQVVADDAVEDPARQISLSE
AFRQNPEIQLDEYIEEILEPVEFGRIGAQAAKQVIFQKIRDAEREQILNDFLERKEYMVTGTIKRMERGNAIIESGRVEA
VLPRDQMIPKENLRVGDRVRAYLHRIDRTTRGPQLILSRIVPEFLIKLFELEVPEIEEGLLEIKVAARDPGSRAKIAVKS
NDQRVDPIGTCVGMRGSRVQAVTGELAGERVDIILWSDDPATFVINALAPAEISSILVDEEKHSMDIVVDEGNLAQAIGR
GGQNVRLASELTGWELNIMTMEESQAKNEEEFSVLRHLFMEKLDVDEEVADILAQEGFTTLEEVAYVPLSEMMEIEAFDE
QTVNELRNRARNALLTEAIVSEEKVEHIAEDLLALEGMDSQTARELAAKGVSTQEDLADLAVDDLVEMVEMDTERARQLI
MAARAPWFAPADRA

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=494, Percent_Identity=52.6315789473684, Blast_Score=514, Evalue=1e-147,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR003583
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 55657; Mature: 55526

Theoretical pI: Translated: 4.29; Mature: 4.29

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSREVLLLVDALAREKNVEKNIVFAALELALASATKKRFNEDADVRVSIDHQTGDYQSLR
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHH
RWQVVADDAVEDPARQISLSEAFRQNPEIQLDEYIEEILEPVEFGRIGAQAAKQVIFQKI
HHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDAEREQILNDFLERKEYMVTGTIKRMERGNAIIESGRVEAVLPRDQMIPKENLRVGDRV
HHHHHHHHHHHHHHHHHEEHHHHHHHHHHCCHHHCCCCEEEECCHHHCCCCCCCCHHHHH
RAYLHRIDRTTRGPQLILSRIVPEFLIKLFELEVPEIEEGLLEIKVAARDPGSRAKIAVK
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCEEEEEECCCCCCCCEEEEC
SNDQRVDPIGTCVGMRGSRVQAVTGELAGERVDIILWSDDPATFVINALAPAEISSILVD
CCCCCCCHHHHHHCCCCCEEHHHHHHHCCCEEEEEEECCCCCEEEEECCCHHHHHHHHHC
EEKHSMDIVVDEGNLAQAIGRGGQNVRLASELTGWELNIMTMEESQAKNEEEFSVLRHLF
CCCCCEEEEEECCCHHHHHCCCCCCEEEHHHCCCCEEEEEEECHHHCCCHHHHHHHHHHH
MEKLDVDEEVADILAQEGFTTLEEVAYVPLSEMMEIEAFDEQTVNELRNRARNALLTEAI
HHHCCCCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSEEKVEHIAEDLLALEGMDSQTARELAAKGVSTQEDLADLAVDDLVEMVEMDTERARQL
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHH
IMAARAPWFAPADRA
HHHHCCCCCCCCCCC
>Mature Secondary Structure 
SREVLLLVDALAREKNVEKNIVFAALELALASATKKRFNEDADVRVSIDHQTGDYQSLR
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHH
RWQVVADDAVEDPARQISLSEAFRQNPEIQLDEYIEEILEPVEFGRIGAQAAKQVIFQKI
HHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDAEREQILNDFLERKEYMVTGTIKRMERGNAIIESGRVEAVLPRDQMIPKENLRVGDRV
HHHHHHHHHHHHHHHHHEEHHHHHHHHHHCCHHHCCCCEEEECCHHHCCCCCCCCHHHHH
RAYLHRIDRTTRGPQLILSRIVPEFLIKLFELEVPEIEEGLLEIKVAARDPGSRAKIAVK
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCEEEEEECCCCCCCCEEEEC
SNDQRVDPIGTCVGMRGSRVQAVTGELAGERVDIILWSDDPATFVINALAPAEISSILVD
CCCCCCCHHHHHHCCCCCEEHHHHHHHCCCEEEEEEECCCCCEEEEECCCHHHHHHHHHC
EEKHSMDIVVDEGNLAQAIGRGGQNVRLASELTGWELNIMTMEESQAKNEEEFSVLRHLF
CCCCCEEEEEECCCHHHHHCCCCCCEEEHHHCCCCEEEEEEECHHHCCCHHHHHHHHHHH
MEKLDVDEEVADILAQEGFTTLEEVAYVPLSEMMEIEAFDEQTVNELRNRARNALLTEAI
HHHCCCCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSEEKVEHIAEDLLALEGMDSQTARELAAKGVSTQEDLADLAVDDLVEMVEMDTERARQL
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHH
IMAARAPWFAPADRA
HHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12704232 [H]