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The map label for this gene is truB

Identifier: 82702983

GI number: 82702983

Start: 2147028

End: 2147969

Strand: Reverse

Name: truB

Synonym: Nmul_A1862

Alternate gene names: 82702983

Gene position: 2147969-2147028 (Counterclockwise)

Preceding gene: 82702984

Following gene: 82702980

Centisome position: 67.46

GC content: 55.52

Gene sequence:

>942_bases
ATGTCTCCAGGTAGCAAACGACATATCAGCGGCGTGCTGCTGCTCGATAAAGCCTCCGGTCTTTCTTCCAACCAAGCCCT
GCAGACCGCCAAACGCATTTTTTCTGCTCATAAGGCTGGCCATACCGGTACGCTTGATCCGATGGCGACCGGTTTGCTGC
CCATCTGCTTCGGAGAGGCCACCAAATTTTCATCGGCGCTCCTCGGTGCAGACAAGACATACGAAGCCGTGCTGAGACTT
GGCTATATGAGTACGACCGGTGATGCAGAGGGGGAAATTTCAATTGCTGCCGGTATGGAATCTCAATACGTGGATCTGAC
CAGGGAGAAAATTGAGGCAGTACGCAAAAGCTTTATCGGCGTAATTACGCAAGTGCCGCCCATGTACAGCGCAATCAAGC
ATCGTGGGAAACCCATGTACACCTTTGCCAGGGCAGGAGTGGAAATCGAACGGCAGCCTCGAGCGATAACGATCCACGAC
CTGAGTATCGAGGCGTATCAAGGCAATGAAATGCGCATCCGGGTGACGTGCGGGAGCGGTACCTACATTCGCACGCTGGC
AGAGGACCTGGGACACGCGCTGGGATGCGGCGGCGCTTATCTTACTGCATTGCGCCGGAGCGCACTGGGCGGTTTTGATT
TGCCGCAGGCATACACACTGACTGGACTCGAAGCTATGCCGCCATCTCAACGGGACTCTTGCCTGCTGCCAGCCGATAGT
CTCCTGAGGTCTCTTCCCCCGGTTGTGGTAGATTCCGCTGCGGCTTTATCTCTGCTCCAGGGACGCGCAATCCCCGGTAC
GCACCCGGCAGGGGAAAGCCTTTTACCGGGAAGACAGGTACGGTTGTATGACAAGGCGCAGCGGTTTCTGGGGTTGGGGG
AAATTTCAACGGAAGGTTACATCTCGCCGAAAAGACTCATAAGGTTTGAGCAGTCATTGTGA

Upstream 100 bases:

>100_bases
ATAGTGAACCCTCCCAATTCCCCGCACTATCCGCAGATTCCGCTCTACCTTTGATAGTAACTGCCGGGATTCGGACTGGC
TCCAAGACCTCCTAGCTTAA

Downstream 100 bases:

>100_bases
AGCTTGATTTGCGTGCATGCTATCGGTGACGTCCTTGCCAATACCCCGGTAGCCGGTAAAGCGACCGGAAGAATCGAACA
TCGGCTCACCACTGACCATT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MSPGSKRHISGVLLLDKASGLSSNQALQTAKRIFSAHKAGHTGTLDPMATGLLPICFGEATKFSSALLGADKTYEAVLRL
GYMSTTGDAEGEISIAAGMESQYVDLTREKIEAVRKSFIGVITQVPPMYSAIKHRGKPMYTFARAGVEIERQPRAITIHD
LSIEAYQGNEMRIRVTCGSGTYIRTLAEDLGHALGCGGAYLTALRRSALGGFDLPQAYTLTGLEAMPPSQRDSCLLPADS
LLRSLPPVVVDSAAALSLLQGRAIPGTHPAGESLLPGRQVRLYDKAQRFLGLGEISTEGYISPKRLIRFEQSL

Sequences:

>Translated_313_residues
MSPGSKRHISGVLLLDKASGLSSNQALQTAKRIFSAHKAGHTGTLDPMATGLLPICFGEATKFSSALLGADKTYEAVLRL
GYMSTTGDAEGEISIAAGMESQYVDLTREKIEAVRKSFIGVITQVPPMYSAIKHRGKPMYTFARAGVEIERQPRAITIHD
LSIEAYQGNEMRIRVTCGSGTYIRTLAEDLGHALGCGGAYLTALRRSALGGFDLPQAYTLTGLEAMPPSQRDSCLLPADS
LLRSLPPVVVDSAAALSLLQGRAIPGTHPAGESLLPGRQVRLYDKAQRFLGLGEISTEGYISPKRLIRFEQSL
>Mature_312_residues
SPGSKRHISGVLLLDKASGLSSNQALQTAKRIFSAHKAGHTGTLDPMATGLLPICFGEATKFSSALLGADKTYEAVLRLG
YMSTTGDAEGEISIAAGMESQYVDLTREKIEAVRKSFIGVITQVPPMYSAIKHRGKPMYTFARAGVEIERQPRAITIHDL
SIEAYQGNEMRIRVTCGSGTYIRTLAEDLGHALGCGGAYLTALRRSALGGFDLPQAYTLTGLEAMPPSQRDSCLLPADSL
LRSLPPVVVDSAAALSLLQGRAIPGTHPAGESLLPGRQVRLYDKAQRFLGLGEISTEGYISPKRLIRFEQSL

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=225, Percent_Identity=32.8888888888889, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI215599015, Length=181, Percent_Identity=29.8342541436464, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI4503337, Length=181, Percent_Identity=29.8342541436464, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI2367200, Length=312, Percent_Identity=41.3461538461538, Blast_Score=236, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI17553978, Length=284, Percent_Identity=27.4647887323944, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6324037, Length=195, Percent_Identity=32.3076923076923, Blast_Score=96, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6323204, Length=271, Percent_Identity=29.1512915129151, Blast_Score=90, Evalue=4e-19,
Organism=Drosophila melanogaster, GI281364189, Length=201, Percent_Identity=32.3383084577114, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI281364187, Length=201, Percent_Identity=32.3383084577114, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI281364185, Length=201, Percent_Identity=32.3383084577114, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI281364183, Length=201, Percent_Identity=32.3383084577114, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI62471759, Length=201, Percent_Identity=32.3383084577114, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI17975520, Length=201, Percent_Identity=32.3383084577114, Blast_Score=85, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_NITMU (Q2Y7W4)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_412549.1
- ProteinModelPortal:   Q2Y7W4
- SMR:   Q2Y7W4
- STRING:   Q2Y7W4
- GeneID:   3786606
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A1862
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- PhylomeDB:   Q2Y7W4
- BioCyc:   NMUL323848:NMUL_A1862-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 33564; Mature: 33433

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: NA

Important sites: ACT_SITE 46-46

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPGSKRHISGVLLLDKASGLSSNQALQTAKRIFSAHKAGHTGTLDPMATGLLPICFGEA
CCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHCCCH
TKFSSALLGADKTYEAVLRLGYMSTTGDAEGEISIAAGMESQYVDLTREKIEAVRKSFIG
HHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
VITQVPPMYSAIKHRGKPMYTFARAGVEIERQPRAITIHDLSIEAYQGNEMRIRVTCGSG
HHHCCCHHHHHHHHCCCCEEEHHHCCCEEECCCCEEEEEEEEEEEEECCEEEEEEEECCC
TYIRTLAEDLGHALGCGGAYLTALRRSALGGFDLPQAYTLTGLEAMPPSQRDSCLLPADS
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCEECCHHH
LLRSLPPVVVDSAAALSLLQGRAIPGTHPAGESLLPGRQVRLYDKAQRFLGLGEISTEGY
HHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCCC
ISPKRLIRFEQSL
CCHHHHHHHHCCC
>Mature Secondary Structure 
SPGSKRHISGVLLLDKASGLSSNQALQTAKRIFSAHKAGHTGTLDPMATGLLPICFGEA
CCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHCCCH
TKFSSALLGADKTYEAVLRLGYMSTTGDAEGEISIAAGMESQYVDLTREKIEAVRKSFIG
HHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
VITQVPPMYSAIKHRGKPMYTFARAGVEIERQPRAITIHDLSIEAYQGNEMRIRVTCGSG
HHHCCCHHHHHHHHCCCCEEEHHHCCCEEECCCCEEEEEEEEEEEEECCEEEEEEEECCC
TYIRTLAEDLGHALGCGGAYLTALRRSALGGFDLPQAYTLTGLEAMPPSQRDSCLLPADS
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCEECCHHH
LLRSLPPVVVDSAAALSLLQGRAIPGTHPAGESLLPGRQVRLYDKAQRFLGLGEISTEGY
HHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCCC
ISPKRLIRFEQSL
CCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA