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The map label for this gene is gloB [H]

Identifier: 82702748

GI number: 82702748

Start: 1860527

End: 1861321

Strand: Direct

Name: gloB [H]

Synonym: Nmul_A1621

Alternate gene names: 82702748

Gene position: 1860527-1861321 (Clockwise)

Preceding gene: 82702744

Following gene: 82702749

Centisome position: 58.43

GC content: 52.33

Gene sequence:

>795_bases
ATGACTACTACTCCCAATATCGTTCCGATTCACGCTTTCAAGGACAATTACATCTGGCTCATCCGCGATCATTATCATGC
TGCCGTTATAGATCCTGGCGATGCGTCCCCTGTCCTGGATTATCTGAGCCGCGAAAAGCTGAAGCTCATCGCCATTCTCA
ATACTCATCACCATCCCGATCACGTCGGCGGAAATGCTGAACTGTTGCGGGAATTTGCCGTACCCGTCTTTGGGCCGGCG
AAAGAATCGATTCCGACGCTCACCCATCGTCTCAAGGAATGCAGTGATATAGAAAACGAAGAAGGCTGTGTATATCTTCC
GGAATTCTCACTCAGCTTCCGCGTGCTCGATATCCCTGGTCACACCTCCGGCCACATCGCCTATTATGGCGCAAACATGC
TCTTTTGTGGTGACACCCTGTTTGGCTGCGGGTGCGGACGATTGTTCGAAGGTACAGCGCAGCAAATGGTCGACTCCCTG
AAAAAACTGGCGGATTTGCCCAAGGATACGCTGATCTATTGCGGCCATGAATACACGCTCAGCAATATCCGCTTCGCAAG
GACAGTCGAACCTGGCAATCAAGCATTGGCGAAGCGCGAAGTGGAAGCCGAAAAATTGCGTAACGAAGGCCTGCCCACCC
TGCCCTCCACCATTGCGCTGGAAAAAGCGACCAATCCGTTCCTGCGCTGTACTGAAGCGGAAGTCATCGAAAGCGCCAGC
AACCATGCGGGGAAACCGTTAAACGACCGTGTCAGCGTATTTGCCGCCATCCGGGAATGGAAAAATCACTTTTGA

Upstream 100 bases:

>100_bases
TCATGAATCAGCAGAAATATCCATCTGCCTGACCTGTTGCTCACCCTTCGGCACCTCCGCCGGCTGCTAGCCGCCTCAAA
ACACCTGAATAGCGCCTATC

Downstream 100 bases:

>100_bases
CATTATCTTTTTCTATACGTCGGTTCTCCAACAGCTTGTGCCGAAAATAAATTTGAATGGTGAAGCAATTGCGTTGTCCT
AATTTTGTGCTTTACTTGGG

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MTTTPNIVPIHAFKDNYIWLIRDHYHAAVIDPGDASPVLDYLSREKLKLIAILNTHHHPDHVGGNAELLREFAVPVFGPA
KESIPTLTHRLKECSDIENEEGCVYLPEFSLSFRVLDIPGHTSGHIAYYGANMLFCGDTLFGCGCGRLFEGTAQQMVDSL
KKLADLPKDTLIYCGHEYTLSNIRFARTVEPGNQALAKREVEAEKLRNEGLPTLPSTIALEKATNPFLRCTEAEVIESAS
NHAGKPLNDRVSVFAAIREWKNHF

Sequences:

>Translated_264_residues
MTTTPNIVPIHAFKDNYIWLIRDHYHAAVIDPGDASPVLDYLSREKLKLIAILNTHHHPDHVGGNAELLREFAVPVFGPA
KESIPTLTHRLKECSDIENEEGCVYLPEFSLSFRVLDIPGHTSGHIAYYGANMLFCGDTLFGCGCGRLFEGTAQQMVDSL
KKLADLPKDTLIYCGHEYTLSNIRFARTVEPGNQALAKREVEAEKLRNEGLPTLPSTIALEKATNPFLRCTEAEVIESAS
NHAGKPLNDRVSVFAAIREWKNHF
>Mature_263_residues
TTTPNIVPIHAFKDNYIWLIRDHYHAAVIDPGDASPVLDYLSREKLKLIAILNTHHHPDHVGGNAELLREFAVPVFGPAK
ESIPTLTHRLKECSDIENEEGCVYLPEFSLSFRVLDIPGHTSGHIAYYGANMLFCGDTLFGCGCGRLFEGTAQQMVDSLK
KLADLPKDTLIYCGHEYTLSNIRFARTVEPGNQALAKREVEAEKLRNEGLPTLPSTIALEKATNPFLRCTEAEVIESASN
HAGKPLNDRVSVFAAIREWKNHF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538320, Length=264, Percent_Identity=39.7727272727273, Blast_Score=179, Evalue=2e-45,
Organism=Homo sapiens, GI94538322, Length=264, Percent_Identity=39.7727272727273, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI14150041, Length=240, Percent_Identity=38.3333333333333, Blast_Score=151, Evalue=5e-37,
Organism=Homo sapiens, GI116642887, Length=271, Percent_Identity=37.2693726937269, Blast_Score=151, Evalue=7e-37,
Organism=Homo sapiens, GI21703352, Length=271, Percent_Identity=37.2693726937269, Blast_Score=151, Evalue=8e-37,
Organism=Homo sapiens, GI46361987, Length=198, Percent_Identity=39.8989898989899, Blast_Score=126, Evalue=3e-29,
Organism=Escherichia coli, GI1786406, Length=261, Percent_Identity=41.7624521072797, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1787158, Length=206, Percent_Identity=35.4368932038835, Blast_Score=92, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17536925, Length=264, Percent_Identity=36.7424242424242, Blast_Score=159, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6320478, Length=283, Percent_Identity=30.3886925795053, Blast_Score=102, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6324614, Length=271, Percent_Identity=30.2583025830258, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356335, Length=271, Percent_Identity=39.8523985239852, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24667711, Length=271, Percent_Identity=39.8523985239852, Blast_Score=168, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24667703, Length=271, Percent_Identity=39.8523985239852, Blast_Score=168, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 29408; Mature: 29277

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTPNIVPIHAFKDNYIWLIRDHYHAAVIDPGDASPVLDYLSREKLKLIAILNTHHHPD
CCCCCCEEEEEEECCCEEEEEECCCEEEEECCCCCCHHHHHHHHCCEEEEEEEECCCCCC
HVGGNAELLREFAVPVFGPAKESIPTLTHRLKECSDIENEEGCVYLPEFSLSFRVLDIPG
CCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEECCC
HTSGHIAYYGANMLFCGDTLFGCGCGRLFEGTAQQMVDSLKKLADLPKDTLIYCGHEYTL
CCCCEEEEECCCEEEECCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEC
SNIRFARTVEPGNQALAKREVEAEKLRNEGLPTLPSTIALEKATNPFLRCTEAEVIESAS
CCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHCCCHHHHHHHHH
NHAGKPLNDRVSVFAAIREWKNHF
HCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTTPNIVPIHAFKDNYIWLIRDHYHAAVIDPGDASPVLDYLSREKLKLIAILNTHHHPD
CCCCCEEEEEEECCCEEEEEECCCEEEEECCCCCCHHHHHHHHCCEEEEEEEECCCCCC
HVGGNAELLREFAVPVFGPAKESIPTLTHRLKECSDIENEEGCVYLPEFSLSFRVLDIPG
CCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEECCC
HTSGHIAYYGANMLFCGDTLFGCGCGRLFEGTAQQMVDSLKKLADLPKDTLIYCGHEYTL
CCCCEEEEECCCEEEECCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEC
SNIRFARTVEPGNQALAKREVEAEKLRNEGLPTLPSTIALEKATNPFLRCTEAEVIESAS
CCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHCCCHHHHHHHHH
NHAGKPLNDRVSVFAAIREWKNHF
HCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA