Click here to switch to the map view.

The map label for this gene is holB [H]

Identifier: 82702515

GI number: 82702515

Start: 1578235

End: 1579341

Strand: Reverse

Name: holB [H]

Synonym: Nmul_A1386

Alternate gene names: 82702515

Gene position: 1579341-1578235 (Counterclockwise)

Preceding gene: 82702516

Following gene: 82702513

Centisome position: 49.6

GC content: 54.65

Gene sequence:

>1107_bases
ATGAGTAATATTTATGGATGGCAGGAAGAAGTCTGGCGGAAACTTACGGGCGCGTTGGGGCACGCCCTGCTGTTACGAGG
CAGAAAGGGACTGGGCAAGCTTGCATTTGCCCGCTATCTGGCGAAGTCCAGACTCTGTGAAAATCGCTCCGTCGAAGGAA
AGGCGTGCGAGGTCTGTGCGAGCTGCCACTGGTTTGAGCAAGGCAATCACCCCGATTTCTGCCTTGTCGAGCCCGAAGCG
GCCACGGCAACGTCTGTCTCGGGGGAAGGAGCCGGGGAGGAGGGTGTGGAAACCGGAGATGAGGCAGAAGTTCAATCTCT
TCCCCGGGCAGTGAATCAGCTCAGCGGCAGTGGCAAGTCCACAAAAAAACCCAGCAGACAGATAAGTATCTCGCAAATAC
GGGAGCTAGGCGACTTCGTCAATATCACCAGCCATCAGAACGGCTATAAGATCATCCTGATCCATCCGGCGGAAACCATG
AGTACGGCTGCTGCCAGTGCCCTTCTGAAGAATCTGGAAGAACCGCCGCTCCAAACGTTGTTCATACTGATAACGCATCA
GGCACAGTATTTATTGCCGACGATCCGCAGCCGTTGCCGCCAGATCATCATGCCCGCCCCCGATGCAGCCTCCGCAGCAC
TGTGGTTAAAACAGCAGGGTGTCAAAGCCCCCGAAAGATGCCTGGCCTCGGCTGGCTATGCTCCCCTGACCGCACTGGAA
TTCGCGAATGAAGATTATCTTGTGCGGCACAGCGCTTTCATCCAGCAAATCAGCACTCCATCAGGTTTCGACGTGCTGGC
ACGGGCAGAGGAAATGCAGAAATCGGACCTTGTCATGGTGGTCAGCTGGCTGCAAAAGTGGTGCTACGATCTGATGAGTT
TTCGTATGGCGCAGAAGGTCCGCTATCATCCGGACATGCTCGCGCAAATAAAACCCCTGGCATCCGGGCTTGATCCGTAT
TCAATGGCAACTTATTTGCGCGCCCTGGATAAAACGCAGCAGCTTGCCCGCCATCCGCTCAATCCAAGATTATTTCTGGA
AGAACTGCTGTTTTCCTATGTGACGATGTTATCCGAGAAATCCAGGAATCGGAGCAAGGCCGGCTGA

Upstream 100 bases:

>100_bases
GGGCAAAAGAATTCCCTGAAAGAATACGGGTGATAGATGCCAACCAGTCCCTGGAAGATGTCAGGCGGTCAGTTGAAAAA
AGTATTGCAACGATTTACGC

Downstream 100 bases:

>100_bases
CAGAAAGTATCTGACAGAAGACAGAAGTTTGCCATGACTGCTTCTGGAATAGATCATGAACAATCCTTGATATCCTTCCC
ATCCTTCCGCGGAATATCGC

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MSNIYGWQEEVWRKLTGALGHALLLRGRKGLGKLAFARYLAKSRLCENRSVEGKACEVCASCHWFEQGNHPDFCLVEPEA
ATATSVSGEGAGEEGVETGDEAEVQSLPRAVNQLSGSGKSTKKPSRQISISQIRELGDFVNITSHQNGYKIILIHPAETM
STAAASALLKNLEEPPLQTLFILITHQAQYLLPTIRSRCRQIIMPAPDAASAALWLKQQGVKAPERCLASAGYAPLTALE
FANEDYLVRHSAFIQQISTPSGFDVLARAEEMQKSDLVMVVSWLQKWCYDLMSFRMAQKVRYHPDMLAQIKPLASGLDPY
SMATYLRALDKTQQLARHPLNPRLFLEELLFSYVTMLSEKSRNRSKAG

Sequences:

>Translated_368_residues
MSNIYGWQEEVWRKLTGALGHALLLRGRKGLGKLAFARYLAKSRLCENRSVEGKACEVCASCHWFEQGNHPDFCLVEPEA
ATATSVSGEGAGEEGVETGDEAEVQSLPRAVNQLSGSGKSTKKPSRQISISQIRELGDFVNITSHQNGYKIILIHPAETM
STAAASALLKNLEEPPLQTLFILITHQAQYLLPTIRSRCRQIIMPAPDAASAALWLKQQGVKAPERCLASAGYAPLTALE
FANEDYLVRHSAFIQQISTPSGFDVLARAEEMQKSDLVMVVSWLQKWCYDLMSFRMAQKVRYHPDMLAQIKPLASGLDPY
SMATYLRALDKTQQLARHPLNPRLFLEELLFSYVTMLSEKSRNRSKAG
>Mature_367_residues
SNIYGWQEEVWRKLTGALGHALLLRGRKGLGKLAFARYLAKSRLCENRSVEGKACEVCASCHWFEQGNHPDFCLVEPEAA
TATSVSGEGAGEEGVETGDEAEVQSLPRAVNQLSGSGKSTKKPSRQISISQIRELGDFVNITSHQNGYKIILIHPAETMS
TAAASALLKNLEEPPLQTLFILITHQAQYLLPTIRSRCRQIIMPAPDAASAALWLKQQGVKAPERCLASAGYAPLTALEF
ANEDYLVRHSAFIQQISTPSGFDVLARAEEMQKSDLVMVVSWLQKWCYDLMSFRMAQKVRYHPDMLAQIKPLASGLDPYS
MATYLRALDKTQQLARHPLNPRLFLEELLFSYVTMLSEKSRNRSKAG

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=220, Percent_Identity=28.1818181818182, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40885; Mature: 40754

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNIYGWQEEVWRKLTGALGHALLLRGRKGLGKLAFARYLAKSRLCENRSVEGKACEVCA
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
SCHWFEQGNHPDFCLVEPEAATATSVSGEGAGEEGVETGDEAEVQSLPRAVNQLSGSGKS
HHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
TKKPSRQISISQIRELGDFVNITSHQNGYKIILIHPAETMSTAAASALLKNLEEPPLQTL
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCHHHH
FILITHQAQYLLPTIRSRCRQIIMPAPDAASAALWLKQQGVKAPERCLASAGYAPLTALE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHH
FANEDYLVRHSAFIQQISTPSGFDVLARAEEMQKSDLVMVVSWLQKWCYDLMSFRMAQKV
HCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYHPDMLAQIKPLASGLDPYSMATYLRALDKTQQLARHPLNPRLFLEELLFSYVTMLSEK
HCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
SRNRSKAG
HCCCCCCC
>Mature Secondary Structure 
SNIYGWQEEVWRKLTGALGHALLLRGRKGLGKLAFARYLAKSRLCENRSVEGKACEVCA
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
SCHWFEQGNHPDFCLVEPEAATATSVSGEGAGEEGVETGDEAEVQSLPRAVNQLSGSGKS
HHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
TKKPSRQISISQIRELGDFVNITSHQNGYKIILIHPAETMSTAAASALLKNLEEPPLQTL
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCHHHH
FILITHQAQYLLPTIRSRCRQIIMPAPDAASAALWLKQQGVKAPERCLASAGYAPLTALE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHH
FANEDYLVRHSAFIQQISTPSGFDVLARAEEMQKSDLVMVVSWLQKWCYDLMSFRMAQKV
HCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYHPDMLAQIKPLASGLDPYSMATYLRALDKTQQLARHPLNPRLFLEELLFSYVTMLSEK
HCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
SRNRSKAG
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]