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The map label for this gene is fliN [H]

Identifier: 82702482

GI number: 82702482

Start: 1541938

End: 1542393

Strand: Direct

Name: fliN [H]

Synonym: Nmul_A1353

Alternate gene names: 82702482

Gene position: 1541938-1542393 (Clockwise)

Preceding gene: 82702481

Following gene: 82702483

Centisome position: 48.42

GC content: 51.32

Gene sequence:

>456_bases
ATGAGTGAAGTTGAACGCCGCGATCTGTCCAGGCGGCCCTTTGTGAATAAAACTGAGGAGATGAAAGTGTCTGTAACACC
AGCCAATTCCGCAGCTCGAACGCCGGCGGATCAGGATGAGACAATTGGGGCCGGGGAGACTGGCTCGGGGCAGATATTCG
AGCAATTCCAGAATACCCAGAAGGTACAGATCCAGAATGACATTGATCTAGTCATGGATATACCCGTTCAACTCACTGTG
CAGTTGGGGCGCACCAAAATCGCCATCAAGAACCTGTTGCAATTGGCGCAGGGTTCCGTGGTGGAGCTGGATGGGCTGGC
GGGAGAACCGATGGATGTTCTGGTAAACGGTTATCTGATTGCTCAAGGCGAAGTTGTGGTAGTCAATGAGAAGTTTGGCA
TACGTCTGACCGATATCATCACGCCGAGTGAACGGATACGCAAATTAAACCGCTAA

Upstream 100 bases:

>100_bases
GCAAGCGTGGATGGGGTACCGGTCATGGAATGCAAATACGGAATTTCGAATGGGCGCTACGCTTTGCGTATGGAGAAAAT
GCTCGCTTCCGGGAACAGTA

Downstream 100 bases:

>100_bases
TCATGAAAAGAAAACATCAGAACCCGTTTTTGCCCCGGGCGGTGCTCCGTAATTCTCTTTTAGCGGCAGCGCTCTGGCAT
TTCGCCTCGCCGGCCATGGC

Product: surface presentation of antigens (SPOA) protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 151; Mature: 150

Protein sequence:

>151_residues
MSEVERRDLSRRPFVNKTEEMKVSVTPANSAARTPADQDETIGAGETGSGQIFEQFQNTQKVQIQNDIDLVMDIPVQLTV
QLGRTKIAIKNLLQLAQGSVVELDGLAGEPMDVLVNGYLIAQGEVVVVNEKFGIRLTDIITPSERIRKLNR

Sequences:

>Translated_151_residues
MSEVERRDLSRRPFVNKTEEMKVSVTPANSAARTPADQDETIGAGETGSGQIFEQFQNTQKVQIQNDIDLVMDIPVQLTV
QLGRTKIAIKNLLQLAQGSVVELDGLAGEPMDVLVNGYLIAQGEVVVVNEKFGIRLTDIITPSERIRKLNR
>Mature_150_residues
SEVERRDLSRRPFVNKTEEMKVSVTPANSAARTPADQDETIGAGETGSGQIFEQFQNTQKVQIQNDIDLVMDIPVQLTVQ
LGRTKIAIKNLLQLAQGSVVELDGLAGEPMDVLVNGYLIAQGEVVVVNEKFGIRLTDIITPSERIRKLNR

Specific function: FliN is one of three proteins (FliG, FliN, FliM) that form a switch complex that is proposed to be located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the motor

COG id: COG1886

COG function: function code NU; Flagellar motor switch/type III secretory pathway protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side. Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliN/MopA/SpaO family [H]

Homologues:

Organism=Escherichia coli, GI1788256, Length=100, Percent_Identity=67, Blast_Score=145, Evalue=2e-36,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012826
- InterPro:   IPR001172
- InterPro:   IPR001543 [H]

Pfam domain/function: PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 16675; Mature: 16544

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVERRDLSRRPFVNKTEEMKVSVTPANSAARTPADQDETIGAGETGSGQIFEQFQNTQ
CCCCHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCC
KVQIQNDIDLVMDIPVQLTVQLGRTKIAIKNLLQLAQGSVVELDGLAGEPMDVLVNGYLI
EEEEECCCEEEEECCEEEEEEECCCHHHHHHHHHHHCCCEEEECCCCCCCHHEEEECEEE
AQGEVVVVNEKFGIRLTDIITPSERIRKLNR
ECCCEEEEECCCCEEEEEEECCHHHHHHCCC
>Mature Secondary Structure 
SEVERRDLSRRPFVNKTEEMKVSVTPANSAARTPADQDETIGAGETGSGQIFEQFQNTQ
CCCHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCC
KVQIQNDIDLVMDIPVQLTVQLGRTKIAIKNLLQLAQGSVVELDGLAGEPMDVLVNGYLI
EEEEECCCEEEEECCEEEEEEECCCHHHHHHHHHHHCCCEEEECCCCCCCHHEEEECEEE
AQGEVVVVNEKFGIRLTDIITPSERIRKLNR
ECCCEEEEECCCCEEEEEEECCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2651416; 9097040; 9278503 [H]