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The map label for this gene is fliM [H]

Identifier: 82702481

GI number: 82702481

Start: 1540965

End: 1541945

Strand: Direct

Name: fliM [H]

Synonym: Nmul_A1352

Alternate gene names: 82702481

Gene position: 1540965-1541945 (Clockwise)

Preceding gene: 82702480

Following gene: 82702482

Centisome position: 48.39

GC content: 53.21

Gene sequence:

>981_bases
ATGAGAGAGGATTTCCTTTCGCGCGAGGAGGTGGATGCTCTTCTCAGGGGAGTGAGCAGTGGATCCACGACAAGCGAACA
GAGTACGGCTAATCCCGGTTTCCGCCCCTATAACCTTGCGACACAGGAACGCATCGTACGGGGGCGCATGCCCACCCTCG
AAATCATCAATGAGCGTTTTGCGCGCCTCCTGCGCCTCGGACTGTTCAATTTCATGCGCCGCAGCGCAAATGTCACCGCG
GGACCGGTAAGGGTTATGAAATTCAGCGAATTCGTGCGCAACCTCGCTGCACCAAGCAACCTGAATCTCGTGCATATAAA
ACCTTTGCGCGGCACCTCGCTATTTGTATTCGACCCGACACTGGTATTTCTTGTGGTTGACAACCTGTTCGGCGGCGATG
GGCGCTTTCTTCCCAAGACTGAAGGCCGCGATTTCACTCAGACCGAGCAGCGCATCATCCAGCGCTTGCTCAACCTGGTC
TTCGACAGCTATGGCAAGTCCTGGCATCCGGTTTATCCGATCGAGTTTGAGTATCTGCGGGCAGAAACGAATACCCAGTT
TGCCAATGTTGCGACACCGAATGAGGTCGTGGTTGCCACCACCTTCGAGGTCAATTTTGGTTCCGTAGTAGGGGAGATGC
ACATCTGCATGCCTTATTCCATTCTCGAACCGATCCGCGATCTGCTGTCGAGCACTTTGCAAGGTGAGGCATTGGAGGTA
GATAAACGCTGGGTCGGTCGCCTCTCAAAACAGGTGCAGTCGGCTGAAGTGACACTACTCGCAAATCTGACGCATGCCCA
GGTAAGACTCGATCAGATCCTGAATATGAAGGTTGGGGATGTTATTCCCTTGGATATTCCGGAATTCGTTACTGCAAGCG
TGGATGGGGTACCGGTCATGGAATGCAAATACGGAATTTCGAATGGGCGCTACGCTTTGCGTATGGAGAAAATGCTCGCT
TCCGGGAACAGTAATGAGTGA

Upstream 100 bases:

>100_bases
TCTGAACTCACTGCCGAGATAAATCACCAGTTCAGCGAGGAGCGTGTGACAGGATCGGTTGAGAACGTGCTTTTTACTTC
GTTCGTGATTCAGTAGCTCC

Downstream 100 bases:

>100_bases
AGTTGAACGCCGCGATCTGTCCAGGCGGCCCTTTGTGAATAAAACTGAGGAGATGAAAGTGTCTGTAACACCAGCCAATT
CCGCAGCTCGAACGCCGGCG

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MREDFLSREEVDALLRGVSSGSTTSEQSTANPGFRPYNLATQERIVRGRMPTLEIINERFARLLRLGLFNFMRRSANVTA
GPVRVMKFSEFVRNLAAPSNLNLVHIKPLRGTSLFVFDPTLVFLVVDNLFGGDGRFLPKTEGRDFTQTEQRIIQRLLNLV
FDSYGKSWHPVYPIEFEYLRAETNTQFANVATPNEVVVATTFEVNFGSVVGEMHICMPYSILEPIRDLLSSTLQGEALEV
DKRWVGRLSKQVQSAEVTLLANLTHAQVRLDQILNMKVGDVIPLDIPEFVTASVDGVPVMECKYGISNGRYALRMEKMLA
SGNSNE

Sequences:

>Translated_326_residues
MREDFLSREEVDALLRGVSSGSTTSEQSTANPGFRPYNLATQERIVRGRMPTLEIINERFARLLRLGLFNFMRRSANVTA
GPVRVMKFSEFVRNLAAPSNLNLVHIKPLRGTSLFVFDPTLVFLVVDNLFGGDGRFLPKTEGRDFTQTEQRIIQRLLNLV
FDSYGKSWHPVYPIEFEYLRAETNTQFANVATPNEVVVATTFEVNFGSVVGEMHICMPYSILEPIRDLLSSTLQGEALEV
DKRWVGRLSKQVQSAEVTLLANLTHAQVRLDQILNMKVGDVIPLDIPEFVTASVDGVPVMECKYGISNGRYALRMEKMLA
SGNSNE
>Mature_326_residues
MREDFLSREEVDALLRGVSSGSTTSEQSTANPGFRPYNLATQERIVRGRMPTLEIINERFARLLRLGLFNFMRRSANVTA
GPVRVMKFSEFVRNLAAPSNLNLVHIKPLRGTSLFVFDPTLVFLVVDNLFGGDGRFLPKTEGRDFTQTEQRIIQRLLNLV
FDSYGKSWHPVYPIEFEYLRAETNTQFANVATPNEVVVATTFEVNFGSVVGEMHICMPYSILEPIRDLLSSTLQGEALEV
DKRWVGRLSKQVQSAEVTLLANLTHAQVRLDQILNMKVGDVIPLDIPEFVTASVDGVPVMECKYGISNGRYALRMEKMLA
SGNSNE

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=321, Percent_Identity=48.2866043613707, Blast_Score=329, Evalue=1e-91,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 36654; Mature: 36654

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREDFLSREEVDALLRGVSSGSTTSEQSTANPGFRPYNLATQERIVRGRMPTLEIINERF
CCCHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHH
ARLLRLGLFNFMRRSANVTAGPVRVMKFSEFVRNLAAPSNLNLVHIKPLRGTSLFVFDPT
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEECCC
LVFLVVDNLFGGDGRFLPKTEGRDFTQTEQRIIQRLLNLVFDSYGKSWHPVYPIEFEYLR
EEEHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEE
AETNTQFANVATPNEVVVATTFEVNFGSVVGEMHICMPYSILEPIRDLLSSTLQGEALEV
ECCCCCEEECCCCCCEEEEEEEEECHHHHHCCHHEECCHHHHHHHHHHHHHHHCCCHHHH
DKRWVGRLSKQVQSAEVTLLANLTHAQVRLDQILNMKVGDVIPLDIPEFVTASVDGVPVM
HHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHCCCCCCEECCCCHHHHHCCCCCCEEE
ECKYGISNGRYALRMEKMLASGNSNE
EEECCCCCCEEHHHHHHHHHCCCCCC
>Mature Secondary Structure
MREDFLSREEVDALLRGVSSGSTTSEQSTANPGFRPYNLATQERIVRGRMPTLEIINERF
CCCHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHH
ARLLRLGLFNFMRRSANVTAGPVRVMKFSEFVRNLAAPSNLNLVHIKPLRGTSLFVFDPT
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEECCC
LVFLVVDNLFGGDGRFLPKTEGRDFTQTEQRIIQRLLNLVFDSYGKSWHPVYPIEFEYLR
EEEHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEE
AETNTQFANVATPNEVVVATTFEVNFGSVVGEMHICMPYSILEPIRDLLSSTLQGEALEV
ECCCCCEEECCCCCCEEEEEEEEECHHHHHCCHHEECCHHHHHHHHHHHHHHHCCCHHHH
DKRWVGRLSKQVQSAEVTLLANLTHAQVRLDQILNMKVGDVIPLDIPEFVTASVDGVPVM
HHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHCCCCCCEECCCCHHHHHCCCCCCEEE
ECKYGISNGRYALRMEKMLASGNSNE
EEECCCCCCEEHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 3519573; 9097040; 9278503; 2651416 [H]