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The map label for this gene is aprF [H]

Identifier: 82702411

GI number: 82702411

Start: 1474942

End: 1476339

Strand: Direct

Name: aprF [H]

Synonym: Nmul_A1282

Alternate gene names: 82702411

Gene position: 1474942-1476339 (Clockwise)

Preceding gene: 82702410

Following gene: 82702412

Centisome position: 46.32

GC content: 56.58

Gene sequence:

>1398_bases
ATGACACCCGCCGCGCGCGTTCTGATTGCTGCCGCCATAGGGCTGGGGAGCATGCTTCATGCGGGCCACGCATCCGCACT
GGGGCTGATGGAGGCATATGAAGCCGCCGTGGCGAACGATCCGGCTTATCGGGCGGCGATTCATGAAAACAGGGCAGGTC
AGGAGTTTACGGCGATAGGCCTTTCCGGCCTGCTTCCCTCCCTTTCTGCAAATTTCTCGATAAATCAGAACCAGCAGGAT
TTCACACGTCAGTCCATTACTCTCCATCGCAGCTATGAGAGCCAAACCGCTGCCATCCAGCTGAGGCAGCCCATCATCAA
CCTGGCTGCATTCGCGGGTTATAAGCAGGGAATCGCAAAGACGAGCCAGAGCAATGCCGATTTTTCCGGGCAGATGCAGG
AACTTATCCTGCGCCTCGTGAGCGCTTATGTGGAAGCAAAATACGCTGAGGATGGGTTGGCTCTGGCGGTAGCGCAATCG
ACTGCGTACGCGGAGCAGCGGCAGGCAAACGAGCGTATGCTCGAGAAAGGAGAGGGGACAAAAACGGAGGCTCTGGAAAC
CCAGGCCAGCTACGACCTCGCACAGGCGCAGGTTCTCGAAGCGGAGGACAATGTGGCAATGAAGCGCGATGTACTTGCTG
CTATCGTAGGCGAGGAAATCAGCGCGCTCGATCCATTATCCGACGACTTCCGCGTCAAGCCGATGCAGCCGGGAAGGTTT
GATGACTGGAAGGACCTCGCGCTCGAGAACAATCCCGATATCGCCGGTCAGCGTCATGCCGTGGATGTGGCGCGCGAAGA
GATCAACAAGCAGCGCGCGGGTCATTTTCCGCGGCTTGACGCGGTAGCAGCCATCACGCGCAACAAGTCGGACAGCCCTA
TCCTCGTCGGTATCGATGCGCTCACGCAGAGCATAGGCGTGCAAGTAAATCTGCCTTTATACGCAGGAGGCAACGTCAGC
GCGATGACAAGCCAGGCAGCGTCAAACTACGAGAAGGCAAGAGCCGATCTCGATACCACCATCAGTGAGACGCTTGTCGA
ACTGCGCAAGCAGTTCAATCTTGCGCTGACGAGTACATCTCGTATTGCGGCGTTTGCGAAATCGGTTGATTCGGCAAATT
TGCTCATCGAAGCCACGACGAAAAGCATCAGAGGGGGCGTGCGGACCAATCTTGATCTTTTGAATGCGCAGCAACGGCTG
TTCGAAGCGAAACGTGATCTTGCGGAGGCACGCTATGGCTACCTCCTGGCCTATCTTCGCCTGCGGAGAGCCGCCGGAAT
AGTGGGATTCGAAGATCTCCACGACATCGCAGGATATTTCATCCGTGTCGGTAACTCTCCCGCGCCAATCAACACTGCCC
GCGCGAAGAAACAGGAATCGTTGCAACAACTTGAATAA

Upstream 100 bases:

>100_bases
ACTCTTCGTAAAGACGGGCGAACGCACCATGATGAATTACATTCTCAAGCCGATTCTGGATCATTTCAAAATGGCGATGA
CAGAGGAGTAGGGTGCCAGC

Downstream 100 bases:

>100_bases
CCTGAACTATGTATGCTTCCGAACAGAGCCAATGTATTTTCCAAGCTACGATAAATCATTCTCTGCTTTTAACTTTAACA
AATTGAGATAAGAAACCCTC

Product: Type I secretion outer membrane protein TolC

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MTPAARVLIAAAIGLGSMLHAGHASALGLMEAYEAAVANDPAYRAAIHENRAGQEFTAIGLSGLLPSLSANFSINQNQQD
FTRQSITLHRSYESQTAAIQLRQPIINLAAFAGYKQGIAKTSQSNADFSGQMQELILRLVSAYVEAKYAEDGLALAVAQS
TAYAEQRQANERMLEKGEGTKTEALETQASYDLAQAQVLEAEDNVAMKRDVLAAIVGEEISALDPLSDDFRVKPMQPGRF
DDWKDLALENNPDIAGQRHAVDVAREEINKQRAGHFPRLDAVAAITRNKSDSPILVGIDALTQSIGVQVNLPLYAGGNVS
AMTSQAASNYEKARADLDTTISETLVELRKQFNLALTSTSRIAAFAKSVDSANLLIEATTKSIRGGVRTNLDLLNAQQRL
FEAKRDLAEARYGYLLAYLRLRRAAGIVGFEDLHDIAGYFIRVGNSPAPINTARAKKQESLQQLE

Sequences:

>Translated_465_residues
MTPAARVLIAAAIGLGSMLHAGHASALGLMEAYEAAVANDPAYRAAIHENRAGQEFTAIGLSGLLPSLSANFSINQNQQD
FTRQSITLHRSYESQTAAIQLRQPIINLAAFAGYKQGIAKTSQSNADFSGQMQELILRLVSAYVEAKYAEDGLALAVAQS
TAYAEQRQANERMLEKGEGTKTEALETQASYDLAQAQVLEAEDNVAMKRDVLAAIVGEEISALDPLSDDFRVKPMQPGRF
DDWKDLALENNPDIAGQRHAVDVAREEINKQRAGHFPRLDAVAAITRNKSDSPILVGIDALTQSIGVQVNLPLYAGGNVS
AMTSQAASNYEKARADLDTTISETLVELRKQFNLALTSTSRIAAFAKSVDSANLLIEATTKSIRGGVRTNLDLLNAQQRL
FEAKRDLAEARYGYLLAYLRLRRAAGIVGFEDLHDIAGYFIRVGNSPAPINTARAKKQESLQQLE
>Mature_464_residues
TPAARVLIAAAIGLGSMLHAGHASALGLMEAYEAAVANDPAYRAAIHENRAGQEFTAIGLSGLLPSLSANFSINQNQQDF
TRQSITLHRSYESQTAAIQLRQPIINLAAFAGYKQGIAKTSQSNADFSGQMQELILRLVSAYVEAKYAEDGLALAVAQST
AYAEQRQANERMLEKGEGTKTEALETQASYDLAQAQVLEAEDNVAMKRDVLAAIVGEEISALDPLSDDFRVKPMQPGRFD
DWKDLALENNPDIAGQRHAVDVAREEINKQRAGHFPRLDAVAAITRNKSDSPILVGIDALTQSIGVQVNLPLYAGGNVSA
MTSQAASNYEKARADLDTTISETLVELRKQFNLALTSTSRIAAFAKSVDSANLLIEATTKSIRGGVRTNLDLLNAQQRLF
EAKRDLAEARYGYLLAYLRLRRAAGIVGFEDLHDIAGYFIRVGNSPAPINTARAKKQESLQQLE

Specific function: Involved in the secretion of alkaline protease [H]

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI87082199, Length=423, Percent_Identity=26.9503546099291, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010130 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 50390; Mature: 50259

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPAARVLIAAAIGLGSMLHAGHASALGLMEAYEAAVANDPAYRAAIHENRAGQEFTAIG
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHH
LSGLLPSLSANFSINQNQQDFTRQSITLHRSYESQTAAIQLRQPIINLAAFAGYKQGIAK
HHHHCCCCCCCCEECCCHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TSQSNADFSGQMQELILRLVSAYVEAKYAEDGLALAVAQSTAYAEQRQANERMLEKGEGT
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCC
KTEALETQASYDLAQAQVLEAEDNVAMKRDVLAAIVGEEISALDPLSDDFRVKPMQPGRF
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCC
DDWKDLALENNPDIAGQRHAVDVAREEINKQRAGHFPRLDAVAAITRNKSDSPILVGIDA
CCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEEHHH
LTQSIGVQVNLPLYAGGNVSAMTSQAASNYEKARADLDTTISETLVELRKQFNLALTSTS
HHHHHCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECHH
RIAAFAKSVDSANLLIEATTKSIRGGVRTNLDLLNAQQRLFEAKRDLAEARYGYLLAYLR
HHHHHHHCCCCCCEEEEECHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRRAAGIVGFEDLHDIAGYFIRVGNSPAPINTARAKKQESLQQLE
HHHHCCCCCHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TPAARVLIAAAIGLGSMLHAGHASALGLMEAYEAAVANDPAYRAAIHENRAGQEFTAIG
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHH
LSGLLPSLSANFSINQNQQDFTRQSITLHRSYESQTAAIQLRQPIINLAAFAGYKQGIAK
HHHHCCCCCCCCEECCCHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TSQSNADFSGQMQELILRLVSAYVEAKYAEDGLALAVAQSTAYAEQRQANERMLEKGEGT
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCC
KTEALETQASYDLAQAQVLEAEDNVAMKRDVLAAIVGEEISALDPLSDDFRVKPMQPGRF
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCC
DDWKDLALENNPDIAGQRHAVDVAREEINKQRAGHFPRLDAVAAITRNKSDSPILVGIDA
CCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEEHHH
LTQSIGVQVNLPLYAGGNVSAMTSQAASNYEKARADLDTTISETLVELRKQFNLALTSTS
HHHHHCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECHH
RIAAFAKSVDSANLLIEATTKSIRGGVRTNLDLLNAQQRLFEAKRDLAEARYGYLLAYLR
HHHHHHHCCCCCCEEEEECHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRRAAGIVGFEDLHDIAGYFIRVGNSPAPINTARAKKQESLQQLE
HHHHCCCCCHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1427098; 10984043 [H]