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The map label for this gene is aglA [H]

Identifier: 82702380

GI number: 82702380

Start: 1437431

End: 1439116

Strand: Reverse

Name: aglA [H]

Synonym: Nmul_A1251

Alternate gene names: 82702380

Gene position: 1439116-1437431 (Counterclockwise)

Preceding gene: 82702381

Following gene: 82702379

Centisome position: 45.19

GC content: 50.18

Gene sequence:

>1686_bases
ATGATTTTGGGTCCACCCGCCCATTCCATGAGTCGCACTGCCCCTGACAATTCGAACGCTGAAGACGAATGGTGGAAGAA
AACCACCGTTTATCATGTCTATGTGCGTTCTTTTTACGACTCCAATGGCGATGGAATCGGAGATATCCAGGGCATCATCG
AAAAACTGGATTATCTGCACGATCTCGGATATGAAACAATCTGGGTGTCGCCCTTTACGCAAAGTCCACAAAAGGACTTC
GGCTACGACATCAGCGATTACCTCTCCATCTCTCCTGAATATGGCGATATGCCGCTCTTTGAGAAACTGGTTGAAGAGGT
GCACCGGCGCAGCATGAAACTGATATTCGACCTGGTGCTGAATCACACTTCGAGCGAGCATTCCTGGTTCATCGAATCCG
CAAGCTCGCGCGACAATCCCAAAGCCGACTGGTACGTCTGGAAAGATGGAAAAGGCAAAAAGGGTTTGAGGCGCCCAAAT
AACTGGCGCGCCATGGCAGGTAACAAAGCCTGGACTTACCATCCGCGACGGAAGCAATTCTATTACACCGCTTTCCTCCC
TTTTCAGCCCGACCTGAACTACCACAATCCTGAAGTCAAACAGGCGATGTTCGAGGTCATCAGATTCTGGCTGAACAAGG
GGGTGGATGGATTCCGTCTCGATATCATCAGTGCCATCTACGAGGACTCAGAATTGCGCAGCAACCCTCCCAGTCCCCGT
TTGACCCCATCGGATAAATCGCTGTCCATCTTTTTCCAGAACCTGAAAAACAATTTTCTCCACGAAAAGAGCTTCGAATT
CGCTATCGAGCTGCGGCGCGTAGTGGATGAGTTCGATAACCCCAAAAGGGTGCTTATCGGGGAATCACATGGAGATGAAG
CACTGATCCATCGCTTCTGTCGAAACGATGGGCAGCATGGATTGCACGCTGTTTTTCTTTTCAAGGCCATTTCCACACCT
TTCAAGGCGGAAAAATACCGCGAAATGCTGATGACATTCGAGAAGCATTTTCCCGAACCGCTGATACCCACTCTCGTCTT
TGCCAACCATGACCGCAACCGTGTCATCAGCCGCCTGGGAGGGAGTATAGAAAAGGCAAAACTGCTGGCCCTGTTCCAGT
TTACCTGCCGGGGGATTCCCTTCACCTACTTCGGTGATGAGATTGGCATACCTCGGGTAAGAATTCCCCTGAAAGACGGA
AAAGATGCGATTGCCATCCAGCATAAATGGGTACCGCAGTTTCTGGTTGATCGCAGCAGTGAAATTCTCAATCTAGACGA
GTGCCGTACCCCGATGCTATGGAATGAAAGGCCCAGGGCAGGCTTTTGTGGGAGTTCAGCAGAGCCCTGGTTGCCGGTGG
CAGACAGCTTCAGGGAAATAAATGTGGAAAAACAGATTTCGGAACCGCATTCCCTTCTCAATTTTTACAGGAAAATCCTC
CTGTTTCGCAACAGGACGCCAAGCTTGCATGCGGGGCGCCTTGAAATCTTGCATGACCTCTGCAACCGGAAAATTCTCGC
CTACCGCAGAATATTCAATGAAGAGAAGCACGTAGTGCTCCTCAACATGTCCCGCCAGCGGGTTAAAATCCCCTTGAATA
AACCCGTACTGCTTTCCACGCATCCTCAAAGCCCTGTGCATCAATTACAACCTTTCGAAGGGCGCATCATCAACGAATCG
CATTGA

Upstream 100 bases:

>100_bases
TATTTCGCATATGCCCGCAAGCACAGCCATCTTGGAAAACAACAGGCAAACTAGTGCTTTCAGATTAAAATGACCGACAT
GTTTTCCCAATCCCGGTACC

Downstream 100 bases:

>100_bases
CAACGGCAGCGGGAATCTTTCTTTCTCGCTGAGAAGAATGCGGGCTAACCCTGTCAGTTTCATTTGAATGAGTGTTTTCA
ATATCTACTAGGAGTATGAC

Product: alpha amylase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 561; Mature: 561

Protein sequence:

>561_residues
MILGPPAHSMSRTAPDNSNAEDEWWKKTTVYHVYVRSFYDSNGDGIGDIQGIIEKLDYLHDLGYETIWVSPFTQSPQKDF
GYDISDYLSISPEYGDMPLFEKLVEEVHRRSMKLIFDLVLNHTSSEHSWFIESASSRDNPKADWYVWKDGKGKKGLRRPN
NWRAMAGNKAWTYHPRRKQFYYTAFLPFQPDLNYHNPEVKQAMFEVIRFWLNKGVDGFRLDIISAIYEDSELRSNPPSPR
LTPSDKSLSIFFQNLKNNFLHEKSFEFAIELRRVVDEFDNPKRVLIGESHGDEALIHRFCRNDGQHGLHAVFLFKAISTP
FKAEKYREMLMTFEKHFPEPLIPTLVFANHDRNRVISRLGGSIEKAKLLALFQFTCRGIPFTYFGDEIGIPRVRIPLKDG
KDAIAIQHKWVPQFLVDRSSEILNLDECRTPMLWNERPRAGFCGSSAEPWLPVADSFREINVEKQISEPHSLLNFYRKIL
LFRNRTPSLHAGRLEILHDLCNRKILAYRRIFNEEKHVVLLNMSRQRVKIPLNKPVLLSTHPQSPVHQLQPFEGRIINES
H

Sequences:

>Translated_561_residues
MILGPPAHSMSRTAPDNSNAEDEWWKKTTVYHVYVRSFYDSNGDGIGDIQGIIEKLDYLHDLGYETIWVSPFTQSPQKDF
GYDISDYLSISPEYGDMPLFEKLVEEVHRRSMKLIFDLVLNHTSSEHSWFIESASSRDNPKADWYVWKDGKGKKGLRRPN
NWRAMAGNKAWTYHPRRKQFYYTAFLPFQPDLNYHNPEVKQAMFEVIRFWLNKGVDGFRLDIISAIYEDSELRSNPPSPR
LTPSDKSLSIFFQNLKNNFLHEKSFEFAIELRRVVDEFDNPKRVLIGESHGDEALIHRFCRNDGQHGLHAVFLFKAISTP
FKAEKYREMLMTFEKHFPEPLIPTLVFANHDRNRVISRLGGSIEKAKLLALFQFTCRGIPFTYFGDEIGIPRVRIPLKDG
KDAIAIQHKWVPQFLVDRSSEILNLDECRTPMLWNERPRAGFCGSSAEPWLPVADSFREINVEKQISEPHSLLNFYRKIL
LFRNRTPSLHAGRLEILHDLCNRKILAYRRIFNEEKHVVLLNMSRQRVKIPLNKPVLLSTHPQSPVHQLQPFEGRIINES
H
>Mature_561_residues
MILGPPAHSMSRTAPDNSNAEDEWWKKTTVYHVYVRSFYDSNGDGIGDIQGIIEKLDYLHDLGYETIWVSPFTQSPQKDF
GYDISDYLSISPEYGDMPLFEKLVEEVHRRSMKLIFDLVLNHTSSEHSWFIESASSRDNPKADWYVWKDGKGKKGLRRPN
NWRAMAGNKAWTYHPRRKQFYYTAFLPFQPDLNYHNPEVKQAMFEVIRFWLNKGVDGFRLDIISAIYEDSELRSNPPSPR
LTPSDKSLSIFFQNLKNNFLHEKSFEFAIELRRVVDEFDNPKRVLIGESHGDEALIHRFCRNDGQHGLHAVFLFKAISTP
FKAEKYREMLMTFEKHFPEPLIPTLVFANHDRNRVISRLGGSIEKAKLLALFQFTCRGIPFTYFGDEIGIPRVRIPLKDG
KDAIAIQHKWVPQFLVDRSSEILNLDECRTPMLWNERPRAGFCGSSAEPWLPVADSFREINVEKQISEPHSLLNFYRKIL
LFRNRTPSLHAGRLEILHDLCNRKILAYRRIFNEEKHVVLLNMSRQRVKIPLNKPVLLSTHPQSPVHQLQPFEGRIINES
H

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=509, Percent_Identity=31.237721021611, Blast_Score=247, Evalue=2e-65,
Organism=Escherichia coli, GI1790687, Length=565, Percent_Identity=31.5044247787611, Blast_Score=273, Evalue=1e-74,
Organism=Escherichia coli, GI1786604, Length=503, Percent_Identity=21.0735586481113, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1789995, Length=161, Percent_Identity=29.8136645962733, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI32565753, Length=526, Percent_Identity=25.4752851711027, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI25147709, Length=547, Percent_Identity=24.1316270566728, Blast_Score=119, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6321726, Length=584, Percent_Identity=33.5616438356164, Blast_Score=302, Evalue=9e-83,
Organism=Saccharomyces cerevisiae, GI6322241, Length=586, Percent_Identity=33.4470989761092, Blast_Score=295, Evalue=9e-81,
Organism=Saccharomyces cerevisiae, GI6322021, Length=586, Percent_Identity=33.4470989761092, Blast_Score=295, Evalue=9e-81,
Organism=Saccharomyces cerevisiae, GI6324416, Length=585, Percent_Identity=32.991452991453, Blast_Score=295, Evalue=1e-80,
Organism=Saccharomyces cerevisiae, GI6319776, Length=592, Percent_Identity=32.6013513513513, Blast_Score=276, Evalue=5e-75,
Organism=Saccharomyces cerevisiae, GI6321731, Length=592, Percent_Identity=32.6013513513513, Blast_Score=276, Evalue=6e-75,
Organism=Saccharomyces cerevisiae, GI6322245, Length=578, Percent_Identity=31.4878892733564, Blast_Score=264, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24583749, Length=536, Percent_Identity=35.4477611940299, Blast_Score=298, Evalue=9e-81,
Organism=Drosophila melanogaster, GI24583747, Length=536, Percent_Identity=35.4477611940299, Blast_Score=297, Evalue=1e-80,
Organism=Drosophila melanogaster, GI45549022, Length=556, Percent_Identity=34.5323741007194, Blast_Score=297, Evalue=1e-80,
Organism=Drosophila melanogaster, GI24586589, Length=523, Percent_Identity=34.4168260038241, Blast_Score=288, Evalue=8e-78,
Organism=Drosophila melanogaster, GI24583745, Length=521, Percent_Identity=34.5489443378119, Blast_Score=288, Evalue=9e-78,
Organism=Drosophila melanogaster, GI24586593, Length=517, Percent_Identity=34.0425531914894, Blast_Score=287, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24586599, Length=532, Percent_Identity=32.3308270676692, Blast_Score=275, Evalue=4e-74,
Organism=Drosophila melanogaster, GI221330053, Length=535, Percent_Identity=32.1495327102804, Blast_Score=272, Evalue=4e-73,
Organism=Drosophila melanogaster, GI24586597, Length=549, Percent_Identity=30.6010928961749, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24586591, Length=518, Percent_Identity=32.046332046332, Blast_Score=266, Evalue=4e-71,
Organism=Drosophila melanogaster, GI24586587, Length=486, Percent_Identity=30.4526748971193, Blast_Score=236, Evalue=4e-62,
Organism=Drosophila melanogaster, GI281360393, Length=457, Percent_Identity=32.1663019693654, Blast_Score=223, Evalue=3e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.20 [H]

Molecular weight: Translated: 65482; Mature: 65482

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILGPPAHSMSRTAPDNSNAEDEWWKKTTVYHVYVRSFYDSNGDGIGDIQGIIEKLDYLH
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHEEEEEEEHHHCCCCCCCHHHHHHHHHHHHHH
DLGYETIWVSPFTQSPQKDFGYDISDYLSISPEYGDMPLFEKLVEEVHRRSMKLIFDLVL
HCCCCEEEECCCCCCCCHHCCCCHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
NHTSSEHSWFIESASSRDNPKADWYVWKDGKGKKGLRRPNNWRAMAGNKAWTYHPRRKQF
CCCCCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCCCCEEECCCCCEE
YYTAFLPFQPDLNYHNPEVKQAMFEVIRFWLNKGVDGFRLDIISAIYEDSELRSNPPSPR
EEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHCCCCCCCC
LTPSDKSLSIFFQNLKNNFLHEKSFEFAIELRRVVDEFDNPKRVLIGESHGDEALIHRFC
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHH
RNDGQHGLHAVFLFKAISTPFKAEKYREMLMTFEKHFPEPLIPTLVFANHDRNRVISRLG
HCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHC
GSIEKAKLLALFQFTCRGIPFTYFGDEIGIPRVRIPLKDGKDAIAIQHKWVPQFLVDRSS
CCHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEECCCCCCEEEEEECCCHHHHHCCCC
EILNLDECRTPMLWNERPRAGFCGSSAEPWLPVADSFREINVEKQISEPHSLLNFYRKIL
CCEEHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHCCHHHHHHHHHHHH
LFRNRTPSLHAGRLEILHDLCNRKILAYRRIFNEEKHVVLLNMSRQRVKIPLNKPVLLST
HHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECCCCEEECCCCCCEEEEC
HPQSPVHQLQPFEGRIINESH
CCCCHHHHCCCCCCEECCCCC
>Mature Secondary Structure
MILGPPAHSMSRTAPDNSNAEDEWWKKTTVYHVYVRSFYDSNGDGIGDIQGIIEKLDYLH
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHEEEEEEEHHHCCCCCCCHHHHHHHHHHHHHH
DLGYETIWVSPFTQSPQKDFGYDISDYLSISPEYGDMPLFEKLVEEVHRRSMKLIFDLVL
HCCCCEEEECCCCCCCCHHCCCCHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
NHTSSEHSWFIESASSRDNPKADWYVWKDGKGKKGLRRPNNWRAMAGNKAWTYHPRRKQF
CCCCCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCCCCEEECCCCCEE
YYTAFLPFQPDLNYHNPEVKQAMFEVIRFWLNKGVDGFRLDIISAIYEDSELRSNPPSPR
EEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHCCCCCCCC
LTPSDKSLSIFFQNLKNNFLHEKSFEFAIELRRVVDEFDNPKRVLIGESHGDEALIHRFC
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHH
RNDGQHGLHAVFLFKAISTPFKAEKYREMLMTFEKHFPEPLIPTLVFANHDRNRVISRLG
HCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHC
GSIEKAKLLALFQFTCRGIPFTYFGDEIGIPRVRIPLKDGKDAIAIQHKWVPQFLVDRSS
CCHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEECCCCCCEEEEEECCCHHHHHCCCC
EILNLDECRTPMLWNERPRAGFCGSSAEPWLPVADSFREINVEKQISEPHSLLNFYRKIL
CCEEHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHCCHHHHHHHHHHHH
LFRNRTPSLHAGRLEILHDLCNRKILAYRRIFNEEKHVVLLNMSRQRVKIPLNKPVLLST
HHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECCCCEEECCCCCCEEEEC
HPQSPVHQLQPFEGRIINESH
CCCCHHHHCCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10400573; 11481430 [H]