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The map label for this gene is ligD [H]

Identifier: 82702306

GI number: 82702306

Start: 1355243

End: 1357804

Strand: Direct

Name: ligD [H]

Synonym: Nmul_A1177

Alternate gene names: 82702306

Gene position: 1355243-1357804 (Clockwise)

Preceding gene: 82702305

Following gene: 82702312

Centisome position: 42.56

GC content: 53.71

Gene sequence:

>2562_bases
ATGACAAATGATGACTCGCTTAAAACTTACCGGGCCAAACGTGATTTCTCGGTAACACCTGAGCCAAGCGGCGTTGCCAG
TGGCAAGACAGCCATCAAGAGTACTGCCAAGGTTTTGTCGTTTGTTGTCCAGAAGCACTGGGCAACGCGTCTGCACTACG
ACTTCAGGCTGGAACTGGAAGGAACGATGAAGAGCTGGGCTGTTCCCAAAGGCCCCAGTTTCGACCCTAACGACAAACGC
ATGGCGGTCCACGTCGAAGACCATCCCATTTCCTATAACAGTTTTGAAGGCGAGATACCCTCAGGTCAGTATGGCGCCGG
TAAAGTCATTATTTGGGATAAGGGAACCTGGATTCCTCTCGAAGATCCGCACAAGGGGTATCGTGACGGCAAGCTGAAAT
TCGAGCTGCGGGGGCTCAAGCTGAAAGGGCACTGGGCCTTGGTGCGCATGAGAAAGGAAGAAAACAAAAAGCAGGAACCC
TGGCTGCTGATAAAAGAAAAGGATGAATATGTCCGTTCATCGCGGGAGTATAGCGTGGTGGACGAAATGCCGGACAGTGT
GGCCGAACTTGACGTGCCAGACGTGCCACAACCTTCCAAAGCTTCCGCAACATCGGGAAGCAGCTCACCCAAAAGCAGTT
CGATATTCCGCAAGGGAACGAAAACGACCTTGCCGGACAATGCGCGCAAGGCTGAGCTTCCGCGGACATTGACGCCGGAG
CTCGCCACGCTGGTGGACGAGCCTCCTGTGGATCCGGAAGAGTGGATTTACGAAATCAAGTTTGACGGGTACCGGATGTT
GAGCAGAATAGAGGGAGGAGAAGTCCGCCTCATAACCCGAAACGGAAATGACTGGTCTCATAAGCTGCCGCATCTGATAA
AAGCGTTGGGAAAGCTCAAACTGAAGTCGTCCTGGCTCGATGGCGAAATTGCGGTGCTGGATGAAAACGGTATTCCCGAT
TTCCAGTCGTTGCAGAATGCCTTCGACAGCGCGCATACCGAGAGCATCATTTATTATCTCTTCGATGTGCCCTTTTACGA
GGGATATGATTTGCGGGACGCTTCCCTTGTCGAACGCCGCGCCTTACTGCAAAAACTGTTTGAGGCACACGCCCCTGATG
AAATACGCTTCAGCGACGTTTTCGATGTTTCTCCAGCGGAAATTCTCGATTCCGCTTGCCGGCTTGGACTGGAAGGAGTG
ATCGGCAAACGTAAAACCTCGGTTTATGTCTCGCGCCGATCCCAGGACTGGATAAAGCTCAAATGTAAAAACCGCCAGGA
ATTCGTTATCGGCGGATATACAGATCCCAAAGGTACGCGGAAGGGTATTGGTTCTCTTCTGCTCGGTTATTATGATGAAG
ACCGAAAACTCAGATATGCAGGGAATGTAGGCGCGGGATTCAGCGATAAGACGCTGGAGGACCTCAGGGCAAGGTTGGAC
AGAATTGCTTTGACGGATAGCCCTTTTGTGAAAGCAAATGGCCTCGATAAAAACGCGCATTGGGTCGAACCCCGGCTTAT
CGCGGAAGTTTCCTTCGGTGAATGGACGCGAGGAGGTCACATTCGTCACTCGGTATTCCACGGTTTGAGATCCGACAAAA
AAGCGGATGTCATTGTTCGCGAGAAGCCCGTGCATCCCGCGGCGAAGTCGGCGCCTGCAAGCGCCATTCCAAAATCCCTG
AAAGTGACGAACCCGGACCGAGTGATCGATCCCTCCACCGGGTTTACAAAGGTCGATCTCATACGCTACTACGCGCTGGT
AGCGCCTTTGATGCTGCCTCACCTCGCACGGCGGCCGGTTTCACTCGTGCGTGCTCCCGAGGGGGTATCGGGCGAACTCT
TTTTTCAGAAGCATTGGGAGAAAGAGAACATGCCGGGGATCGAGCAGCTTGATCCATCGCTCGACCCCGACCATCCTCCG
CTGCTCGAAGTTTCAACCGCTGAAGGCCTTCTTTCCGCAGTGCAGATGAACGTGATCGAGTTTCATACGTGGAACGCCAC
CAAGAATGTCATTGGCAAGCCTGACAGAATCGCGTTCGACCTGGACCCCGGCAGCGGAGTCGAATGGGCCCTCATCCAGG
AGTCGGCGCAGCTCGTCAATGTCTTTCTCAGGGAACTGGGGTTGAAATCTTTTCTGAAAACGAGTGGGGGCAAAGGCCTG
CACATCGTCGTGCCGATCAGGAGGCTGCGTGATTGGGATACGGTGAAAGGTTTCTCTCAGGCCATCGTACAGCATCTGGC
TAAAACCATTCCGACGCGTTTCACCGCAAAAAGCGGGCCTCGCAACCGGGTGGGAAAGATCTTCATCGATTACCTGCGCA
ATGGGTTCGGAGCCACCACCGTATCCGCCTGGTCAGTACGCGCCCGGCCTGGATTGGGCGTTTCTGTACCGCTGGCGTGG
GAAGAGCTGTCCTCGCTGAAAAGCAGTACTCGCTGGTCCGCTTCCAATATTCATGAACGCCTGGATAAGGGTAATGACCC
CTGGGCCGACTACGCAGAGACGAATCAATCGATAGTTCCTGCCATGAAAAAGCTGGGATTCACCCCTTCATCAAAGGCAT
GA

Upstream 100 bases:

>100_bases
CCGGCGACCTGTCAGAAAGCCCGCCAAATCGACGACTGCCTCATCTGCGCCTGCCCGCAGCGCACACAAGCGCCGGGCGG
TATAGGGCGAATTCGATAGA

Downstream 100 bases:

>100_bases
AGGCGCTATCCGGACAAATCTCATGGCTTCACTCCTGTAACCAGGCTGTAGTAACCGAAGGCCGGATGCACTTCGATGCC
CTCAAATCCCGCTTCCGCCA

Product: ATP-dependent DNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 853; Mature: 852

Protein sequence:

>853_residues
MTNDDSLKTYRAKRDFSVTPEPSGVASGKTAIKSTAKVLSFVVQKHWATRLHYDFRLELEGTMKSWAVPKGPSFDPNDKR
MAVHVEDHPISYNSFEGEIPSGQYGAGKVIIWDKGTWIPLEDPHKGYRDGKLKFELRGLKLKGHWALVRMRKEENKKQEP
WLLIKEKDEYVRSSREYSVVDEMPDSVAELDVPDVPQPSKASATSGSSSPKSSSIFRKGTKTTLPDNARKAELPRTLTPE
LATLVDEPPVDPEEWIYEIKFDGYRMLSRIEGGEVRLITRNGNDWSHKLPHLIKALGKLKLKSSWLDGEIAVLDENGIPD
FQSLQNAFDSAHTESIIYYLFDVPFYEGYDLRDASLVERRALLQKLFEAHAPDEIRFSDVFDVSPAEILDSACRLGLEGV
IGKRKTSVYVSRRSQDWIKLKCKNRQEFVIGGYTDPKGTRKGIGSLLLGYYDEDRKLRYAGNVGAGFSDKTLEDLRARLD
RIALTDSPFVKANGLDKNAHWVEPRLIAEVSFGEWTRGGHIRHSVFHGLRSDKKADVIVREKPVHPAAKSAPASAIPKSL
KVTNPDRVIDPSTGFTKVDLIRYYALVAPLMLPHLARRPVSLVRAPEGVSGELFFQKHWEKENMPGIEQLDPSLDPDHPP
LLEVSTAEGLLSAVQMNVIEFHTWNATKNVIGKPDRIAFDLDPGSGVEWALIQESAQLVNVFLRELGLKSFLKTSGGKGL
HIVVPIRRLRDWDTVKGFSQAIVQHLAKTIPTRFTAKSGPRNRVGKIFIDYLRNGFGATTVSAWSVRARPGLGVSVPLAW
EELSSLKSSTRWSASNIHERLDKGNDPWADYAETNQSIVPAMKKLGFTPSSKA

Sequences:

>Translated_853_residues
MTNDDSLKTYRAKRDFSVTPEPSGVASGKTAIKSTAKVLSFVVQKHWATRLHYDFRLELEGTMKSWAVPKGPSFDPNDKR
MAVHVEDHPISYNSFEGEIPSGQYGAGKVIIWDKGTWIPLEDPHKGYRDGKLKFELRGLKLKGHWALVRMRKEENKKQEP
WLLIKEKDEYVRSSREYSVVDEMPDSVAELDVPDVPQPSKASATSGSSSPKSSSIFRKGTKTTLPDNARKAELPRTLTPE
LATLVDEPPVDPEEWIYEIKFDGYRMLSRIEGGEVRLITRNGNDWSHKLPHLIKALGKLKLKSSWLDGEIAVLDENGIPD
FQSLQNAFDSAHTESIIYYLFDVPFYEGYDLRDASLVERRALLQKLFEAHAPDEIRFSDVFDVSPAEILDSACRLGLEGV
IGKRKTSVYVSRRSQDWIKLKCKNRQEFVIGGYTDPKGTRKGIGSLLLGYYDEDRKLRYAGNVGAGFSDKTLEDLRARLD
RIALTDSPFVKANGLDKNAHWVEPRLIAEVSFGEWTRGGHIRHSVFHGLRSDKKADVIVREKPVHPAAKSAPASAIPKSL
KVTNPDRVIDPSTGFTKVDLIRYYALVAPLMLPHLARRPVSLVRAPEGVSGELFFQKHWEKENMPGIEQLDPSLDPDHPP
LLEVSTAEGLLSAVQMNVIEFHTWNATKNVIGKPDRIAFDLDPGSGVEWALIQESAQLVNVFLRELGLKSFLKTSGGKGL
HIVVPIRRLRDWDTVKGFSQAIVQHLAKTIPTRFTAKSGPRNRVGKIFIDYLRNGFGATTVSAWSVRARPGLGVSVPLAW
EELSSLKSSTRWSASNIHERLDKGNDPWADYAETNQSIVPAMKKLGFTPSSKA
>Mature_852_residues
TNDDSLKTYRAKRDFSVTPEPSGVASGKTAIKSTAKVLSFVVQKHWATRLHYDFRLELEGTMKSWAVPKGPSFDPNDKRM
AVHVEDHPISYNSFEGEIPSGQYGAGKVIIWDKGTWIPLEDPHKGYRDGKLKFELRGLKLKGHWALVRMRKEENKKQEPW
LLIKEKDEYVRSSREYSVVDEMPDSVAELDVPDVPQPSKASATSGSSSPKSSSIFRKGTKTTLPDNARKAELPRTLTPEL
ATLVDEPPVDPEEWIYEIKFDGYRMLSRIEGGEVRLITRNGNDWSHKLPHLIKALGKLKLKSSWLDGEIAVLDENGIPDF
QSLQNAFDSAHTESIIYYLFDVPFYEGYDLRDASLVERRALLQKLFEAHAPDEIRFSDVFDVSPAEILDSACRLGLEGVI
GKRKTSVYVSRRSQDWIKLKCKNRQEFVIGGYTDPKGTRKGIGSLLLGYYDEDRKLRYAGNVGAGFSDKTLEDLRARLDR
IALTDSPFVKANGLDKNAHWVEPRLIAEVSFGEWTRGGHIRHSVFHGLRSDKKADVIVREKPVHPAAKSAPASAIPKSLK
VTNPDRVIDPSTGFTKVDLIRYYALVAPLMLPHLARRPVSLVRAPEGVSGELFFQKHWEKENMPGIEQLDPSLDPDHPPL
LEVSTAEGLLSAVQMNVIEFHTWNATKNVIGKPDRIAFDLDPGSGVEWALIQESAQLVNVFLRELGLKSFLKTSGGKGLH
IVVPIRRLRDWDTVKGFSQAIVQHLAKTIPTRFTAKSGPRNRVGKIFIDYLRNGFGATTVSAWSVRARPGLGVSVPLAWE
ELSSLKSSTRWSASNIHERLDKGNDPWADYAETNQSIVPAMKKLGFTPSSKA

Specific function: Probably involved in the repair of DNA double-strand breaks by non-homologous-end joining (NHEJ) during spore germination [H]

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family [H]

Homologues:

Organism=Homo sapiens, GI4557719, Length=325, Percent_Identity=28.6153846153846, Blast_Score=101, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24762424, Length=326, Percent_Identity=27.3006134969325, Blast_Score=83, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012310
- InterPro:   IPR014146
- InterPro:   IPR014145
- InterPro:   IPR014143 [H]

Pfam domain/function: PF01068 DNA_ligase_A_M [H]

EC number: =6.5.1.1 [H]

Molecular weight: Translated: 95585; Mature: 95454

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS50160 DNA_LIGASE_A3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNDDSLKTYRAKRDFSVTPEPSGVASGKTAIKSTAKVLSFVVQKHWATRLHYDFRLELE
CCCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEC
GTMKSWAVPKGPSFDPNDKRMAVHVEDHPISYNSFEGEIPSGQYGAGKVIIWDKGTWIPL
CCHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCC
EDPHKGYRDGKLKFELRGLKLKGHWALVRMRKEENKKQEPWLLIKEKDEYVRSSREYSVV
CCCCCCCCCCEEEEEEEEEEEECCEEEEEEHHHCCCCCCCEEEEECCHHHHHCCCCEEHH
DEMPDSVAELDVPDVPQPSKASATSGSSSPKSSSIFRKGTKTTLPDNARKAELPRTLTPE
HHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCHHCCCCCCCHH
LATLVDEPPVDPEEWIYEIKFDGYRMLSRIEGGEVRLITRNGNDWSHKLPHLIKALGKLK
HHHHHCCCCCCHHHEEEEEEECHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
LKSSWLDGEIAVLDENGIPDFQSLQNAFDSAHTESIIYYLFDVPFYEGYDLRDASLVERR
HCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHHH
ALLQKLFEAHAPDEIRFSDVFDVSPAEILDSACRLGLEGVIGKRKTSVYVSRRSQDWIKL
HHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHCHHHHCCCCCEEEEEECCCCCEEEE
KCKNRQEFVIGGYTDPKGTRKGIGSLLLGYYDEDRKLRYAGNVGAGFSDKTLEDLRARLD
EECCCCEEEEECCCCCCHHHHHHHHHHHHEECCCCEEEEECCCCCCCCHHHHHHHHHHHH
RIALTDSPFVKANGLDKNAHWVEPRLIAEVSFGEWTRGGHIRHSVFHGLRSDKKADVIVR
HEEECCCCCEEECCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCEEEE
EKPVHPAAKSAPASAIPKSLKVTNPDRVIDPSTGFTKVDLIRYYALVAPLMLPHLARRPV
ECCCCCCCCCCCHHHCCCCCEECCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCH
SLVRAPEGVSGELFFQKHWEKENMPGIEQLDPSLDPDHPPLLEVSTAEGLLSAVQMNVIE
HHHCCCCCCCCCCHHHHHCCCCCCCCHHHCCCCCCCCCCCEEEECHHHHHHHHHHHHEEE
FHTWNATKNVIGKPDRIAFDLDPGSGVEWALIQESAQLVNVFLRELGLKSFLKTSGGKGL
EEECCCCHHHCCCCCCEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
HIVVPIRRLRDWDTVKGFSQAIVQHLAKTIPTRFTAKSGPRNRVGKIFIDYLRNGFGATT
EEEEEHHHCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHCCCCCCE
VSAWSVRARPGLGVSVPLAWEELSSLKSSTRWSASNIHERLDKGNDPWADYAETNQSIVP
EEEEEEECCCCCCEECCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHCCCHHHH
AMKKLGFTPSSKA
HHHHCCCCCCCCC
>Mature Secondary Structure 
TNDDSLKTYRAKRDFSVTPEPSGVASGKTAIKSTAKVLSFVVQKHWATRLHYDFRLELE
CCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEC
GTMKSWAVPKGPSFDPNDKRMAVHVEDHPISYNSFEGEIPSGQYGAGKVIIWDKGTWIPL
CCHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCC
EDPHKGYRDGKLKFELRGLKLKGHWALVRMRKEENKKQEPWLLIKEKDEYVRSSREYSVV
CCCCCCCCCCEEEEEEEEEEEECCEEEEEEHHHCCCCCCCEEEEECCHHHHHCCCCEEHH
DEMPDSVAELDVPDVPQPSKASATSGSSSPKSSSIFRKGTKTTLPDNARKAELPRTLTPE
HHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCHHCCCCCCCHH
LATLVDEPPVDPEEWIYEIKFDGYRMLSRIEGGEVRLITRNGNDWSHKLPHLIKALGKLK
HHHHHCCCCCCHHHEEEEEEECHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
LKSSWLDGEIAVLDENGIPDFQSLQNAFDSAHTESIIYYLFDVPFYEGYDLRDASLVERR
HCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHHH
ALLQKLFEAHAPDEIRFSDVFDVSPAEILDSACRLGLEGVIGKRKTSVYVSRRSQDWIKL
HHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHCHHHHCCCCCEEEEEECCCCCEEEE
KCKNRQEFVIGGYTDPKGTRKGIGSLLLGYYDEDRKLRYAGNVGAGFSDKTLEDLRARLD
EECCCCEEEEECCCCCCHHHHHHHHHHHHEECCCCEEEEECCCCCCCCHHHHHHHHHHHH
RIALTDSPFVKANGLDKNAHWVEPRLIAEVSFGEWTRGGHIRHSVFHGLRSDKKADVIVR
HEEECCCCCEEECCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCEEEE
EKPVHPAAKSAPASAIPKSLKVTNPDRVIDPSTGFTKVDLIRYYALVAPLMLPHLARRPV
ECCCCCCCCCCCHHHCCCCCEECCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCH
SLVRAPEGVSGELFFQKHWEKENMPGIEQLDPSLDPDHPPLLEVSTAEGLLSAVQMNVIE
HHHCCCCCCCCCCHHHHHCCCCCCCCHHHCCCCCCCCCCCEEEECHHHHHHHHHHHHEEE
FHTWNATKNVIGKPDRIAFDLDPGSGVEWALIQESAQLVNVFLRELGLKSFLKTSGGKGL
EEECCCCHHHCCCCCCEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
HIVVPIRRLRDWDTVKGFSQAIVQHLAKTIPTRFTAKSGPRNRVGKIFIDYLRNGFGATT
EEEEEHHHCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHCCCCCCE
VSAWSVRARPGLGVSVPLAWEELSSLKSSTRWSASNIHERLDKGNDPWADYAETNQSIVP
EEEEEEECCCCCCEECCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHCCCHHHH
AMKKLGFTPSSKA
HHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]