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The map label for this gene is cysD [H]

Identifier: 82702277

GI number: 82702277

Start: 1321501

End: 1322502

Strand: Direct

Name: cysD [H]

Synonym: Nmul_A1148

Alternate gene names: 82702277

Gene position: 1321501-1322502 (Clockwise)

Preceding gene: 82702276

Following gene: 82702278

Centisome position: 41.5

GC content: 54.79

Gene sequence:

>1002_bases
ATGGACGTTATTTTGAATCAAATGGATGAATTTGCACCTGTTGCAGGACCGGTTGCAGGTCTTGTGATGAAGAATGAACC
CGTCGTCATCAAGCGGCGTGCCGCGCGACAACTTAGCCACCTTGAAGCACTGGAAAGCGAAGCAATCCATATCATGCGCG
AGGTCGCGGCCGAGTGTGCCAATCCAGCTCTACTCTTTTCCGGTGGCAAGGACTCGATTGTTCTTCTGCGTCTGGCGGAA
AAGGCTTTCCGTCCCGGACGTTTCCCGTTTCCTTTGATGCATGTCGATACCGGCCATAATTTTCCGGAAGTGATCGAGTT
TCGTGACCGCCGTGTCAAACAGCTCGGTGAGCGCTTAATTGTTGCCAGCATGGAAGACTCGATCAAGCAGGGAAGGGTGG
TGCTGCGCTCGGAAAATCAGAGCCGTAATCCCTTTCAATCCGTGACGCTGCTGGATGCCATCGCCGAACACGGTTTCGAT
GCCTGCATTGGCGGCGCCCGGCGCGACGAGGAGAAAGCTCGGGCAAAGGAACGCATATTCTCCTTCCGGGATGAATTCGG
CCAATGGGATCCGAAGAATCAGCGCCCGGAATTATGGGATCTTTACAATGCAAGAGTTCATCCGGGAGAAAATATCCGCG
TATTTCCCATCAGCAACTGGACGGAACTCGATGTGTGGGAATACATTTCACGCGAGGGGCTGGAGGTGCCGCATATCTAT
TTCGCCCACAGGCGTGATGTCATCCGGCGCGGCAGTGGGCTTTTGCCGATATCCCACCTGGTTCAGCCGAAGGAAGGGGA
AAAGGTTGAAAATCTCATGGTGCGCTTCCGGACGGTGGGTGACATGAGCTGCACCTGTCCGGTGGAGTCGGCAGCGGTCG
GTATTGGGGAAATCATTGCAGAGACGGCGGTTACGCGAATTACCGAGCGGGGTGCAACGCGCATGGACGACCAGACATCG
GAGGCTTCCATGGAGTTGCGCAAGAGAGAAGGATATTTCTGA

Upstream 100 bases:

>100_bases
GAATAAAAGACATCGGCGGGCACAGTAGGAATAATAAGCAGGGATAGTAGGGACGAAGGAATTGCGTGGAGCGCCTCCTG
AACTGAATGGGGATTGTTTG

Downstream 100 bases:

>100_bases
GAGGCGTTGCCATCTCCCGAGCGACTCCGGTTTTCCTTTTTTAAGATGACAGCTTCTGAAAGCAGGTAATCTCCGATCTT
GTTTCATCTTTGCTTCAGTT

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MDVILNQMDEFAPVAGPVAGLVMKNEPVVIKRRAARQLSHLEALESEAIHIMREVAAECANPALLFSGGKDSIVLLRLAE
KAFRPGRFPFPLMHVDTGHNFPEVIEFRDRRVKQLGERLIVASMEDSIKQGRVVLRSENQSRNPFQSVTLLDAIAEHGFD
ACIGGARRDEEKARAKERIFSFRDEFGQWDPKNQRPELWDLYNARVHPGENIRVFPISNWTELDVWEYISREGLEVPHIY
FAHRRDVIRRGSGLLPISHLVQPKEGEKVENLMVRFRTVGDMSCTCPVESAAVGIGEIIAETAVTRITERGATRMDDQTS
EASMELRKREGYF

Sequences:

>Translated_333_residues
MDVILNQMDEFAPVAGPVAGLVMKNEPVVIKRRAARQLSHLEALESEAIHIMREVAAECANPALLFSGGKDSIVLLRLAE
KAFRPGRFPFPLMHVDTGHNFPEVIEFRDRRVKQLGERLIVASMEDSIKQGRVVLRSENQSRNPFQSVTLLDAIAEHGFD
ACIGGARRDEEKARAKERIFSFRDEFGQWDPKNQRPELWDLYNARVHPGENIRVFPISNWTELDVWEYISREGLEVPHIY
FAHRRDVIRRGSGLLPISHLVQPKEGEKVENLMVRFRTVGDMSCTCPVESAAVGIGEIIAETAVTRITERGATRMDDQTS
EASMELRKREGYF
>Mature_333_residues
MDVILNQMDEFAPVAGPVAGLVMKNEPVVIKRRAARQLSHLEALESEAIHIMREVAAECANPALLFSGGKDSIVLLRLAE
KAFRPGRFPFPLMHVDTGHNFPEVIEFRDRRVKQLGERLIVASMEDSIKQGRVVLRSENQSRNPFQSVTLLDAIAEHGFD
ACIGGARRDEEKARAKERIFSFRDEFGQWDPKNQRPELWDLYNARVHPGENIRVFPISNWTELDVWEYISREGLEVPHIY
FAHRRDVIRRGSGLLPISHLVQPKEGEKVENLMVRFRTVGDMSCTCPVESAAVGIGEIIAETAVTRITERGATRMDDQTS
EASMELRKREGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=299, Percent_Identity=49.4983277591973, Blast_Score=285, Evalue=3e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 37818; Mature: 37818

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVILNQMDEFAPVAGPVAGLVMKNEPVVIKRRAARQLSHLEALESEAIHIMREVAAECA
CCCHHHHHHHHCCCCCHHHHHEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NPALLFSGGKDSIVLLRLAEKAFRPGRFPFPLMHVDTGHNFPEVIEFRDRRVKQLGERLI
CCEEEEECCCCCEEEEEEHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHH
VASMEDSIKQGRVVLRSENQSRNPFQSVTLLDAIAEHGFDACIGGARRDEEKARAKERIF
HHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHH
SFRDEFGQWDPKNQRPELWDLYNARVHPGENIRVFPISNWTELDVWEYISREGLEVPHIY
HHHHHHCCCCCCCCCCCHHHHHCCEECCCCCEEEEECCCCCCHHHHHHHHHCCCCCCHHH
FAHRRDVIRRGSGLLPISHLVQPKEGEKVENLMVRFRTVGDMSCTCPVESAAVGIGEIIA
HHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHH
ETAVTRITERGATRMDDQTSEASMELRKREGYF
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDVILNQMDEFAPVAGPVAGLVMKNEPVVIKRRAARQLSHLEALESEAIHIMREVAAECA
CCCHHHHHHHHCCCCCHHHHHEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NPALLFSGGKDSIVLLRLAEKAFRPGRFPFPLMHVDTGHNFPEVIEFRDRRVKQLGERLI
CCEEEEECCCCCEEEEEEHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHH
VASMEDSIKQGRVVLRSENQSRNPFQSVTLLDAIAEHGFDACIGGARRDEEKARAKERIF
HHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHH
SFRDEFGQWDPKNQRPELWDLYNARVHPGENIRVFPISNWTELDVWEYISREGLEVPHIY
HHHHHHCCCCCCCCCCCHHHHHCCEECCCCCEEEEECCCCCCHHHHHHHHHCCCCCCHHH
FAHRRDVIRRGSGLLPISHLVQPKEGEKVENLMVRFRTVGDMSCTCPVESAAVGIGEIIA
HHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHH
ETAVTRITERGATRMDDQTSEASMELRKREGYF
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]