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The map label for this gene is nuoF2 [H]

Identifier: 82702225

GI number: 82702225

Start: 1261161

End: 1262438

Strand: Direct

Name: nuoF2 [H]

Synonym: Nmul_A1096

Alternate gene names: 82702225

Gene position: 1261161-1262438 (Clockwise)

Preceding gene: 82702224

Following gene: 82702226

Centisome position: 39.61

GC content: 54.77

Gene sequence:

>1278_bases
ATGAACCAGCCTGCCCAGATACTGCTGGCCGGATTGAATCCATCGGATCCCAACGATTGGCGGCTGAATAATTACGAACA
GCGTGAAGGATATGCTGCGCTCAGAAAAATCCTGAGCGATAAAATTCCTCCGGAAAAGATCATCGAGGAGGTAAAGAAAT
CCGCCTTGCGGGGTCGCGGCGGAGCCGGTTTTCCGACCGGGTTGAAATGGAGTTTCATGCCCAAGCAGTACGAAGGCGAC
AAATACCTGGTATGCAATTCGGATGAAGGCGAACCTGGCACCTTCAAGGATCGTGACATCATGCGTTACAACCCTCATAT
GCTGATCGAGGGCATGCTGATCGGAGCCTATGCGATGGGCATCAGGGCGGGCTACAACTACGTGCATGGCGAAATCTGGG
ACGTCTACGAGCGCATGGAGGAGGCAGTGGATGAAGCCCGTGCGGCGGGTTATCTCGGGGATAACATCCTCGGTTCCGAT
TTCAGCTTCCAGTTATACAACCATCATGGCTACGGCGCCTATATCTGCGGCGAGGAAACAGCGCTGCTCGAGTCGCTGGA
GGGCAAAAAAGGTCAGCCGCGCTTCAAGCCGCCTTTTCCCGCAAGTTTCGGTCTCTATGGTAAACCGACTACCATCAACA
ACACGGAGACATTCGCGTCGGTCCCCTGGATTATCAGGCACGGGGGTGACAAGTTTCTGCAACTGGGTAAGCCAAACAAT
GGCGGAACCAAGATTTTTTCCGTTTCCGGGCACGTGAACAAGCCCGGCAATTATGAAGTGCCTCTTGGCACGCCATTCGC
AGCCTTGCTGGAGATGGCTGGTGGCATGCGCGGCGGACGCAAGCTGAAAGGCTGTATTCCCGGAGGTTCATCCATGCCGG
TATTGCCGGGTGATGTCATGATGCAGACCGACATGGACTATGACTCGATTGCCAAGGCAGGATCATTTCTCGGTTCAGGC
GCGGTCATTATCATGGACGATACGACCTGCATGGTCAGGGCCCTGGAGCGCTTATCCTATTTTTACTATGAAGAATCCTG
CGGCCAGTGCACCCCCTGCCGTGAAGGCACGGGATGGCTTTACCGCATCGTCCACCGGATCGAAACAGGAAAAGGCCGCA
TGGAAGACCTGGATTTGCTCAATAATCTCGCCGACAACATTCAGGGGCGTACCATCTGCGCATTGGGTGACGCGGCCGCC
ATGCCGGTGCGCGCCATGATTCAGCATTTCCGTGACGAATTTGCGTATCACGTCGAACACAAGAAATGCCTGGTGTGA

Upstream 100 bases:

>100_bases
GTGCATGTGGAGATGCGCCGGTTCTCCTGGTCAATAACAAACGCATGTGCAGTTTCATGTCCAACGACAAGATCGACCAG
TTGCTGGAGGATTTGAGCAA

Downstream 100 bases:

>100_bases
CACGGATGAAGTCATGAGTCTTTTCTTGTTTGCGGCTGCTTGTTGCCCCGGAGCACATAAGTAATGATCAATTTCGAAAT
CGACGGTAAACCGGTGTCCG

Product: NADH-quinone oxidoreductase subunit F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MNQPAQILLAGLNPSDPNDWRLNNYEQREGYAALRKILSDKIPPEKIIEEVKKSALRGRGGAGFPTGLKWSFMPKQYEGD
KYLVCNSDEGEPGTFKDRDIMRYNPHMLIEGMLIGAYAMGIRAGYNYVHGEIWDVYERMEEAVDEARAAGYLGDNILGSD
FSFQLYNHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTETFASVPWIIRHGGDKFLQLGKPNN
GGTKIFSVSGHVNKPGNYEVPLGTPFAALLEMAGGMRGGRKLKGCIPGGSSMPVLPGDVMMQTDMDYDSIAKAGSFLGSG
AVIIMDDTTCMVRALERLSYFYYEESCGQCTPCREGTGWLYRIVHRIETGKGRMEDLDLLNNLADNIQGRTICALGDAAA
MPVRAMIQHFRDEFAYHVEHKKCLV

Sequences:

>Translated_425_residues
MNQPAQILLAGLNPSDPNDWRLNNYEQREGYAALRKILSDKIPPEKIIEEVKKSALRGRGGAGFPTGLKWSFMPKQYEGD
KYLVCNSDEGEPGTFKDRDIMRYNPHMLIEGMLIGAYAMGIRAGYNYVHGEIWDVYERMEEAVDEARAAGYLGDNILGSD
FSFQLYNHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTETFASVPWIIRHGGDKFLQLGKPNN
GGTKIFSVSGHVNKPGNYEVPLGTPFAALLEMAGGMRGGRKLKGCIPGGSSMPVLPGDVMMQTDMDYDSIAKAGSFLGSG
AVIIMDDTTCMVRALERLSYFYYEESCGQCTPCREGTGWLYRIVHRIETGKGRMEDLDLLNNLADNIQGRTICALGDAAA
MPVRAMIQHFRDEFAYHVEHKKCLV
>Mature_425_residues
MNQPAQILLAGLNPSDPNDWRLNNYEQREGYAALRKILSDKIPPEKIIEEVKKSALRGRGGAGFPTGLKWSFMPKQYEGD
KYLVCNSDEGEPGTFKDRDIMRYNPHMLIEGMLIGAYAMGIRAGYNYVHGEIWDVYERMEEAVDEARAAGYLGDNILGSD
FSFQLYNHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTETFASVPWIIRHGGDKFLQLGKPNN
GGTKIFSVSGHVNKPGNYEVPLGTPFAALLEMAGGMRGGRKLKGCIPGGSSMPVLPGDVMMQTDMDYDSIAKAGSFLGSG
AVIIMDDTTCMVRALERLSYFYYEESCGQCTPCREGTGWLYRIVHRIETGKGRMEDLDLLNNLADNIQGRTICALGDAAA
MPVRAMIQHFRDEFAYHVEHKKCLV

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=406, Percent_Identity=49.5073891625616, Blast_Score=398, Evalue=1e-111,
Organism=Homo sapiens, GI260656005, Length=406, Percent_Identity=49.5073891625616, Blast_Score=398, Evalue=1e-111,
Organism=Escherichia coli, GI1788620, Length=418, Percent_Identity=42.822966507177, Blast_Score=344, Evalue=6e-96,
Organism=Caenorhabditis elegans, GI17531727, Length=412, Percent_Identity=50.2427184466019, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI19920794, Length=405, Percent_Identity=48.8888888888889, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI45550452, Length=409, Percent_Identity=47.921760391198, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24653819, Length=402, Percent_Identity=37.5621890547264, Blast_Score=259, Evalue=3e-69,
Organism=Drosophila melanogaster, GI24653817, Length=402, Percent_Identity=33.8308457711443, Blast_Score=221, Evalue=1e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 47092; Mature: 47092

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQPAQILLAGLNPSDPNDWRLNNYEQREGYAALRKILSDKIPPEKIIEEVKKSALRGRG
CCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
GAGFPTGLKWSFMPKQYEGDKYLVCNSDEGEPGTFKDRDIMRYNPHMLIEGMLIGAYAMG
CCCCCCCCEEECCCCCCCCCEEEEEECCCCCCCCCCCCCEEEECCCEEEHHHHHHHHHHH
IRAGYNYVHGEIWDVYERMEEAVDEARAAGYLGDNILGSDFSFQLYNHHGYGAYICGEET
HHHCCCHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEEECCHH
ALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTETFASVPWIIRHGGDKFLQLGKPNN
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCEEEECCCCCEEEECCCCC
GGTKIFSVSGHVNKPGNYEVPLGTPFAALLEMAGGMRGGRKLKGCIPGGSSMPVLPGDVM
CCCEEEEECCCCCCCCCEECCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCEE
MQTDMDYDSIAKAGSFLGSGAVIIMDDTTCMVRALERLSYFYYEESCGQCTPCREGTGWL
EECCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
YRIVHRIETGKGRMEDLDLLNNLADNIQGRTICALGDAAAMPVRAMIQHFRDEFAYHVEH
HHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCH
KKCLV
HHCCC
>Mature Secondary Structure
MNQPAQILLAGLNPSDPNDWRLNNYEQREGYAALRKILSDKIPPEKIIEEVKKSALRGRG
CCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
GAGFPTGLKWSFMPKQYEGDKYLVCNSDEGEPGTFKDRDIMRYNPHMLIEGMLIGAYAMG
CCCCCCCCEEECCCCCCCCCEEEEEECCCCCCCCCCCCCEEEECCCEEEHHHHHHHHHHH
IRAGYNYVHGEIWDVYERMEEAVDEARAAGYLGDNILGSDFSFQLYNHHGYGAYICGEET
HHHCCCHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEEECCHH
ALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTETFASVPWIIRHGGDKFLQLGKPNN
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCEEEECCCCCEEEECCCCC
GGTKIFSVSGHVNKPGNYEVPLGTPFAALLEMAGGMRGGRKLKGCIPGGSSMPVLPGDVM
CCCEEEEECCCCCCCCCEECCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCEE
MQTDMDYDSIAKAGSFLGSGAVIIMDDTTCMVRALERLSYFYYEESCGQCTPCREGTGWL
EECCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
YRIVHRIETGKGRMEDLDLLNNLADNIQGRTICALGDAAAMPVRAMIQHFRDEFAYHVEH
HHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCH
KKCLV
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]