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The map label for this gene is nuoB1

Identifier: 82702143

GI number: 82702143

Start: 1173639

End: 1174304

Strand: Direct

Name: nuoB1

Synonym: Nmul_A1014

Alternate gene names: 82702143

Gene position: 1173639-1174304 (Clockwise)

Preceding gene: 82702142

Following gene: 82702144

Centisome position: 36.86

GC content: 56.46

Gene sequence:

>666_bases
ATGCGGTGGTCTCTAAGTAAGCCATCTGACAAACCATCCGGGCCAGCAACGCCCGGGTCTTCAATAATTCGCGATAACGG
TTTTGAGGAAGACGGGCGCCGCAGCGTGTTGCTGACGCGATTGCAAGATCTTGTCGCCTGGGGCCGCAAGAATTCGGTCT
GGCCTTACAACTTTGGCCTCTCGTGTTGTTATGTGGAAATGGCGACCAGCTTCACCAGCAAGTATGACCTTGCCCGCTTT
GGCTCCGAAGTTATCCGCGCCTCTCCTCGCCAAGCCGACCTGATCGTTATTTCCGGTACGGTTTTTATCAAAATGGCGCC
CGTCCTCCAGCGGCTTTACGATCAGATGCTTGAACCGCGCTGGGTTATTTCGATGGGCTCATGCGCGAATTCGGGTGGAA
TGTATGACATTTACAGCGTGGTGCAGGGTGTAGATCGATTCCTCCCCGTGGATGTTTATGTTCCGGGTTGCCCGCCTCGG
CCTGACGCATTCATGCAGGGACTGAACTTGCTTCAGGACGCAATCGGCAAGGAAAAGCGTCCGTTGAGCTGGGTGATTGG
TCCGCAGGGGATCGAGCGACCGGCTGAGGAATCGCAGCGGGATCTCAAGCGTTCCGGCCGAATGTGCGCCACCACCCTGC
GCAGTCCCGACGAGGTACAAAAGTGA

Upstream 100 bases:

>100_bases
GGAGCGGCCCTCGCTTATTTGTGGCGACTGGGGGCGCTGGACTGGGGGCCGCCCAGGCATTCGGCTGGCAGGTTTGCCAA
AGACTCAAGGAGTCCAAATC

Downstream 100 bases:

>100_bases
GCGCAGCCTCCAGCCTTGCCCCACAGCGGTATATGCAGGACATGGGCGGTGCATCGAACGCTTCAGATGTACTGGAAGAA
CTGAAGAACCGCTTTGGCCC

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B 1; NDH-1 subunit B 1

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MRWSLSKPSDKPSGPATPGSSIIRDNGFEEDGRRSVLLTRLQDLVAWGRKNSVWPYNFGLSCCYVEMATSFTSKYDLARF
GSEVIRASPRQADLIVISGTVFIKMAPVLQRLYDQMLEPRWVISMGSCANSGGMYDIYSVVQGVDRFLPVDVYVPGCPPR
PDAFMQGLNLLQDAIGKEKRPLSWVIGPQGIERPAEESQRDLKRSGRMCATTLRSPDEVQK

Sequences:

>Translated_221_residues
MRWSLSKPSDKPSGPATPGSSIIRDNGFEEDGRRSVLLTRLQDLVAWGRKNSVWPYNFGLSCCYVEMATSFTSKYDLARF
GSEVIRASPRQADLIVISGTVFIKMAPVLQRLYDQMLEPRWVISMGSCANSGGMYDIYSVVQGVDRFLPVDVYVPGCPPR
PDAFMQGLNLLQDAIGKEKRPLSWVIGPQGIERPAEESQRDLKRSGRMCATTLRSPDEVQK
>Mature_221_residues
MRWSLSKPSDKPSGPATPGSSIIRDNGFEEDGRRSVLLTRLQDLVAWGRKNSVWPYNFGLSCCYVEMATSFTSKYDLARF
GSEVIRASPRQADLIVISGTVFIKMAPVLQRLYDQMLEPRWVISMGSCANSGGMYDIYSVVQGVDRFLPVDVYVPGCPPR
PDAFMQGLNLLQDAIGKEKRPLSWVIGPQGIERPAEESQRDLKRSGRMCATTLRSPDEVQK

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=182, Percent_Identity=48.3516483516484, Blast_Score=174, Evalue=7e-44,
Organism=Escherichia coli, GI1788624, Length=192, Percent_Identity=69.2708333333333, Blast_Score=304, Evalue=3e-84,
Organism=Escherichia coli, GI1789074, Length=162, Percent_Identity=35.8024691358025, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1788834, Length=122, Percent_Identity=37.7049180327869, Blast_Score=86, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17509685, Length=182, Percent_Identity=47.2527472527472, Blast_Score=172, Evalue=1e-43,
Organism=Drosophila melanogaster, GI18859983, Length=148, Percent_Identity=57.4324324324324, Blast_Score=179, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24642371, Length=148, Percent_Identity=57.4324324324324, Blast_Score=179, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24651058, Length=147, Percent_Identity=55.1020408163265, Blast_Score=171, Evalue=4e-43,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB1_NITMU (Q2YAA4)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411709.1
- ProteinModelPortal:   Q2YAA4
- SMR:   Q2YAA4
- STRING:   Q2YAA4
- GeneID:   3786539
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A1014
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   PRPEAYI
- PhylomeDB:   Q2YAA4
- ProtClustDB:   PRK06411
- BioCyc:   NMUL323848:NMUL_A1014-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 24684; Mature: 24684

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRWSLSKPSDKPSGPATPGSSIIRDNGFEEDGRRSVLLTRLQDLVAWGRKNSVWPYNFGL
CCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
SCCYVEMATSFTSKYDLARFGSEVIRASPRQADLIVISGTVFIKMAPVLQRLYDQMLEPR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHEEHHHHHHHHHHHHHCCCE
WVISMGSCANSGGMYDIYSVVQGVDRFLPVDVYVPGCPPRPDAFMQGLNLLQDAIGKEKR
EEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCC
PLSWVIGPQGIERPAEESQRDLKRSGRMCATTLRSPDEVQK
CCEEEECCCCCCCCHHHHHHHHHHCCCHHHHHCCCCHHHCC
>Mature Secondary Structure
MRWSLSKPSDKPSGPATPGSSIIRDNGFEEDGRRSVLLTRLQDLVAWGRKNSVWPYNFGL
CCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
SCCYVEMATSFTSKYDLARFGSEVIRASPRQADLIVISGTVFIKMAPVLQRLYDQMLEPR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHEEHHHHHHHHHHHHHCCCE
WVISMGSCANSGGMYDIYSVVQGVDRFLPVDVYVPGCPPRPDAFMQGLNLLQDAIGKEKR
EEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCC
PLSWVIGPQGIERPAEESQRDLKRSGRMCATTLRSPDEVQK
CCEEEECCCCCCCCHHHHHHHHHHCCCHHHHHCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA