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The map label for this gene is potF [H]

Identifier: 82702108

GI number: 82702108

Start: 1139170

End: 1140270

Strand: Reverse

Name: potF [H]

Synonym: Nmul_A0979

Alternate gene names: 82702108

Gene position: 1140270-1139170 (Counterclockwise)

Preceding gene: 82702109

Following gene: 82702107

Centisome position: 35.81

GC content: 54.95

Gene sequence:

>1101_bases
ATGAACCCTCCACGACTCCTTTTTTTTCTGCTGGCATCACTGCTCACGTTCGCCGCGGGATGTACTCCCCCGAATCCGGA
TGCGGGCAACAGCGGCAAAAATGTCCTGCACCTGTTCAACTGGAATAACTACATCGCGCCGGAAACCGTTGCGCGCTTCG
AGAAATCCTGTAAGTGCGACCTGTCGCAGGATTATTATGCCGACAACGAGGAAATGCTGGCGAAGCTCGCGGCGGGAGCC
ACCGGTTATGATGTTATCGTTCCCACGGGCAATGCGATCGACACCCTCATCCGCCAGGGAGCGCTGCGGCCGCTGGATAA
ATCGCTCCTGCCCAATTTCAGGAATATCAATCCTGCCTATCTCGATACGGCCTTTGACCCCGGTAACATATACTCGGTCC
CCTACGCCTACACGCTCTCTCTGCTCGGTTTCAACAAGGAGAAGATCGAGCAGCTTGGTCTGCCGACTGATACCTGGGCA
ATCATCTTCGAACCCAAATATCTGGAAAAAATCAGGGGACGGGTGACCGTGCTCGACAGCCAGCGCGAGCTGATGGCCGC
CGCGCTCAAGTATCTGGGCTATTCCGTGAACGATACGGATGAGAGGCATTGGCAGGAGGCCGCCGCTCTGATCGTGCGCG
CCAAACCCTATTGGGCGGCCTTCAGCAATACCAGCTACATCAAGGAACTGGCAGTGGGTAATCTGTGGGTGGCGCACGGT
TATTCCAATGACATGTTCCAGGCGGCGCTCGATGCCCAGAAAACCGGGCGGAAATTCACGATCAGCTATTCGACGCCCAA
AGAGGGAGCAGTGCTGGCAGTGGATAGCATGGTTCTGCACAAAAGCGGGAAACGCCCCGATCTTGCTCACCAGTTCATCA
ATTTCATGCTGGATGGAAAGAATTCCGCCGAACTCACCAATCTCATCGGCTCGGGCAATCCCAATCTCGATGCTTTGCAA
TACATCCAGCCAGAAATTGCAAGCAACAAGGCCATTTTTCCCGATCCGGAACTGATTGCCCGGCTTGAAATGCTGCGCGA
TCTCGATCGCAAGCAGCGGCGACTGTTGAGCCGCTTGTGGACAGAAATTAAACTGCGATAA

Upstream 100 bases:

>100_bases
TGATCATCATTGCCGCCCGGCTTGAATCAAACGCCTCCTCGGAAGAGAGCACGAAACCTCGCAAAACGGTGAAATAAGCG
GTGAAACAAGCGGCGGAATA

Downstream 100 bases:

>100_bases
TCGTTATAATTTCTGCTCATCCATTCAATACAAAAACCATGAGCTACTACCATCGGCATGTATTTTTCTGCGTGAACCAG
CGTGAACCCGGCACCTTGTG

Product: extracellular solute-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MNPPRLLFFLLASLLTFAAGCTPPNPDAGNSGKNVLHLFNWNNYIAPETVARFEKSCKCDLSQDYYADNEEMLAKLAAGA
TGYDVIVPTGNAIDTLIRQGALRPLDKSLLPNFRNINPAYLDTAFDPGNIYSVPYAYTLSLLGFNKEKIEQLGLPTDTWA
IIFEPKYLEKIRGRVTVLDSQRELMAAALKYLGYSVNDTDERHWQEAAALIVRAKPYWAAFSNTSYIKELAVGNLWVAHG
YSNDMFQAALDAQKTGRKFTISYSTPKEGAVLAVDSMVLHKSGKRPDLAHQFINFMLDGKNSAELTNLIGSGNPNLDALQ
YIQPEIASNKAIFPDPELIARLEMLRDLDRKQRRLLSRLWTEIKLR

Sequences:

>Translated_366_residues
MNPPRLLFFLLASLLTFAAGCTPPNPDAGNSGKNVLHLFNWNNYIAPETVARFEKSCKCDLSQDYYADNEEMLAKLAAGA
TGYDVIVPTGNAIDTLIRQGALRPLDKSLLPNFRNINPAYLDTAFDPGNIYSVPYAYTLSLLGFNKEKIEQLGLPTDTWA
IIFEPKYLEKIRGRVTVLDSQRELMAAALKYLGYSVNDTDERHWQEAAALIVRAKPYWAAFSNTSYIKELAVGNLWVAHG
YSNDMFQAALDAQKTGRKFTISYSTPKEGAVLAVDSMVLHKSGKRPDLAHQFINFMLDGKNSAELTNLIGSGNPNLDALQ
YIQPEIASNKAIFPDPELIARLEMLRDLDRKQRRLLSRLWTEIKLR
>Mature_366_residues
MNPPRLLFFLLASLLTFAAGCTPPNPDAGNSGKNVLHLFNWNNYIAPETVARFEKSCKCDLSQDYYADNEEMLAKLAAGA
TGYDVIVPTGNAIDTLIRQGALRPLDKSLLPNFRNINPAYLDTAFDPGNIYSVPYAYTLSLLGFNKEKIEQLGLPTDTWA
IIFEPKYLEKIRGRVTVLDSQRELMAAALKYLGYSVNDTDERHWQEAAALIVRAKPYWAAFSNTSYIKELAVGNLWVAHG
YSNDMFQAALDAQKTGRKFTISYSTPKEGAVLAVDSMVLHKSGKRPDLAHQFINFMLDGKNSAELTNLIGSGNPNLDALQ
YIQPEIASNKAIFPDPELIARLEMLRDLDRKQRRLLSRLWTEIKLR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=339, Percent_Identity=33.3333333333333, Blast_Score=211, Evalue=5e-56,
Organism=Escherichia coli, GI1787367, Length=321, Percent_Identity=30.5295950155763, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1787711, Length=332, Percent_Identity=25.3012048192771, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 41014; Mature: 41014

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPPRLLFFLLASLLTFAAGCTPPNPDAGNSGKNVLHLFNWNNYIAPETVARFEKSCKCD
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCC
LSQDYYADNEEMLAKLAAGATGYDVIVPTGNAIDTLIRQGALRPLDKSLLPNFRNINPAY
CCCCCCCCCHHHHHHHHCCCCCCEEEEECCHHHHHHHHHCCCCCCCHHHCCCCCCCCHHH
LDTAFDPGNIYSVPYAYTLSLLGFNKEKIEQLGLPTDTWAIIFEPKYLEKIRGRVTVLDS
EECCCCCCCEEECCHHHHHHHHCCCHHHHHHCCCCCCCEEEEECCHHHHHHCCCEEEECC
QRELMAAALKYLGYSVNDTDERHWQEAAALIVRAKPYWAAFSNTSYIKELAVGNLWVAHG
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEECCCCEEECCCCHHHHHHHHCCEEEEEC
YSNDMFQAALDAQKTGRKFTISYSTPKEGAVLAVDSMVLHKSGKRPDLAHQFINFMLDGK
CCCHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEHHHHHHCCCCCCHHHHHHHHHHCCCC
NSAELTNLIGSGNPNLDALQYIQPEIASNKAIFPDPELIARLEMLRDLDRKQRRLLSRLW
CHHHHHHHHCCCCCCHHHHHHHCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TEIKLR
HHHCCC
>Mature Secondary Structure
MNPPRLLFFLLASLLTFAAGCTPPNPDAGNSGKNVLHLFNWNNYIAPETVARFEKSCKCD
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCC
LSQDYYADNEEMLAKLAAGATGYDVIVPTGNAIDTLIRQGALRPLDKSLLPNFRNINPAY
CCCCCCCCCHHHHHHHHCCCCCCEEEEECCHHHHHHHHHCCCCCCCHHHCCCCCCCCHHH
LDTAFDPGNIYSVPYAYTLSLLGFNKEKIEQLGLPTDTWAIIFEPKYLEKIRGRVTVLDS
EECCCCCCCEEECCHHHHHHHHCCCHHHHHHCCCCCCCEEEEECCHHHHHHCCCEEEECC
QRELMAAALKYLGYSVNDTDERHWQEAAALIVRAKPYWAAFSNTSYIKELAVGNLWVAHG
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEECCCCEEECCCCHHHHHHHHCCEEEEEC
YSNDMFQAALDAQKTGRKFTISYSTPKEGAVLAVDSMVLHKSGKRPDLAHQFINFMLDGK
CCCHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEHHHHHHCCCCCCHHHHHHHHHHCCCC
NSAELTNLIGSGNPNLDALQYIQPEIASNKAIFPDPELIARLEMLRDLDRKQRRLLSRLW
CHHHHHHHHCCCCCCHHHHHHHCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TEIKLR
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]