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The map label for this gene is pncA [H]

Identifier: 82702035

GI number: 82702035

Start: 1030552

End: 1031133

Strand: Direct

Name: pncA [H]

Synonym: Nmul_A0906

Alternate gene names: 82702035

Gene position: 1030552-1031133 (Clockwise)

Preceding gene: 82702034

Following gene: 82702036

Centisome position: 32.36

GC content: 53.78

Gene sequence:

>582_bases
ATGAAAATGGACATAGGCACCGGTGATGCCCTTATCATAGTGGATGTGCAAAACGACTTTCTGCCCGGCGGAAGCCTTCC
TGTACCGGCGGGAGATGAGGTCATCCCAAACCTTAACCGGTATATCGCAGCTTTTCTTTTTCGCGAATTACCTATTTTCG
CTACTCGCGACTGGCATCCGCCTGATCACTGCTCCTTTCATCAGCAAGGAGGGCCTTGGCCCGCGCACTGTATTGCCGGC
ACGCAGGGCGCGGCATTTCCGGCGAATCTTGAAATCCCCTGCGACACGCATATCATTTCCAAGGCCACCTCCAGGGAGAA
AGATGCTTATTCCGGTTTTTCGGAAACAGAACTGGATGCCATGCTGAAATCCGCAGGAATTTCCCGTGTTTTCATCGGTG
GCCTGGCTACGGATCATTGCGTTCTCAGTACCGTACGGGATGCCTTGAACCAGGGGTATGACATCTTTGTTCTCAAGGAT
GCAGTACGGGGGCTGGATGCCGACGCAACCAGCGCCGCCCTAGAGGAAATGGCCCATCTGGGCGCAAAACTCATCGATTT
CGAAACCATTGTCCCTGCGTGA

Upstream 100 bases:

>100_bases
CCGCGCTTGACAGGTTCGGCGGATTAGACTAAGTCCGGCGCACTCAACCGAAGAATGTAAAGTGCAGGGAGCAAAGCGGC
AAAGGGGATAGGAGATTGCC

Downstream 100 bases:

>100_bases
ACCCACTCTCCAGCCCGCTACTGACCGACCATTACCAGTTGACCATGCTGGAGAGCTACCTTCAGCAGGGAATGCAGGAA
ACTGCGGTTTTCGAGCTGTT

Product: isochorismatase hydrolase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MKMDIGTGDALIIVDVQNDFLPGGSLPVPAGDEVIPNLNRYIAAFLFRELPIFATRDWHPPDHCSFHQQGGPWPAHCIAG
TQGAAFPANLEIPCDTHIISKATSREKDAYSGFSETELDAMLKSAGISRVFIGGLATDHCVLSTVRDALNQGYDIFVLKD
AVRGLDADATSAALEEMAHLGAKLIDFETIVPA

Sequences:

>Translated_193_residues
MKMDIGTGDALIIVDVQNDFLPGGSLPVPAGDEVIPNLNRYIAAFLFRELPIFATRDWHPPDHCSFHQQGGPWPAHCIAG
TQGAAFPANLEIPCDTHIISKATSREKDAYSGFSETELDAMLKSAGISRVFIGGLATDHCVLSTVRDALNQGYDIFVLKD
AVRGLDADATSAALEEMAHLGAKLIDFETIVPA
>Mature_193_residues
MKMDIGTGDALIIVDVQNDFLPGGSLPVPAGDEVIPNLNRYIAAFLFRELPIFATRDWHPPDHCSFHQQGGPWPAHCIAG
TQGAAFPANLEIPCDTHIISKATSREKDAYSGFSETELDAMLKSAGISRVFIGGLATDHCVLSTVRDALNQGYDIFVLKD
AVRGLDADATSAALEEMAHLGAKLIDFETIVPA

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=198, Percent_Identity=35.3535353535354, Blast_Score=112, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6321401, Length=213, Percent_Identity=28.169014084507, Blast_Score=84, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648247, Length=203, Percent_Identity=31.5270935960591, Blast_Score=90, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 20720; Mature: 20720

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMDIGTGDALIIVDVQNDFLPGGSLPVPAGDEVIPNLNRYIAAFLFRELPIFATRDWHP
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCC
PDHCSFHQQGGPWPAHCIAGTQGAAFPANLEIPCDTHIISKATSREKDAYSGFSETELDA
CCCCCCCCCCCCCCHHEECCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHCCCCHHHHHH
MLKSAGISRVFIGGLATDHCVLSTVRDALNQGYDIFVLKDAVRGLDADATSAALEEMAHL
HHHHCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEHHHCCCCCCHHHHHHHHHHHC
GAKLIDFETIVPA
CHHHHCHHHCCCC
>Mature Secondary Structure
MKMDIGTGDALIIVDVQNDFLPGGSLPVPAGDEVIPNLNRYIAAFLFRELPIFATRDWHP
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCC
PDHCSFHQQGGPWPAHCIAGTQGAAFPANLEIPCDTHIISKATSREKDAYSGFSETELDA
CCCCCCCCCCCCCCHHEECCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHCCCCHHHHHH
MLKSAGISRVFIGGLATDHCVLSTVRDALNQGYDIFVLKDAVRGLDADATSAALEEMAHL
HHHHCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEHHHCCCCCCHHHHHHHHHHHC
GAKLIDFETIVPA
CHHHHCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]