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The map label for this gene is panB

Identifier: 82702008

GI number: 82702008

Start: 1000108

End: 1000899

Strand: Reverse

Name: panB

Synonym: Nmul_A0879

Alternate gene names: 82702008

Gene position: 1000899-1000108 (Counterclockwise)

Preceding gene: 82702009

Following gene: 82702007

Centisome position: 31.43

GC content: 56.57

Gene sequence:

>792_bases
ATGCGTATCACACAAGATGTCTTTCAAAAAATGCGGGATGATGGAGAGAAAATCGCCATCGTCACCTGCTACGACGCAAG
TTTTGCGGCATTGCTGGAAAGAGCAGGCGTCGATATCCTGCTGGTGGGGGATTCACTGGGAAATGTGATCCAGGGTGAAG
AGACTACCCTCCCGGTTACTCTGGATGACATGATCTACCATACACTTTGCGTGAAACGCGGCGCAAGCACGGCTTTCATC
ATGACGGACATGCCTTTCGGCACCTCGCAGGTGAGTCCCGAAGAAACCTTCCGCAATGCAGCCGAACTCATGGCAGCAGG
CGCCAACATGGTCAAGATCGAAGGCGGTTCCGTCATGGCCGAAACCGTGGAGTTCCTGACGCAGCGCGGCATTCCCGTCT
GCGCGCACATTGGCCTCACGCCCCAGTCGGTAAACCAGTTGGGGGGCTACAAGGTGCAGGGAAAGACCGACTATGAAGCC
GAGCAGTTGCTGGAGGATGCGGTAGCGCTCGAACAGGCCGGAGCGGGAATGCTTTTGATGGAAGTCGTGCCTGCCGCCCT
TGCAGCGCAGGTTACCCGAACGCTCTCCATTCCTACCATCGGCATCGGCGCCGGGAAGGATTGTTCGGCGCAGGTACTGG
TGCTGTACGACATGCTGGGCATTTATTCAGGCAAAAAGGCCAGGTTCATGAAGAATTTCCTCACGGGCGCGGGCAGCATC
GAGGAGGCTGTGCAAAACTATACCAGGGCGGTCAAAACCGGGGAATTTCCTGGGCCCGAGCATACATTCTGA

Upstream 100 bases:

>100_bases
CGAGGGAATATTTCAATTTCGAAAGATGAGCAAACCGGAAAACAGGAACCCGAAAATGGGTGGGATGATCTATCCGATAC
ATGCAACCAGGAGCTGAACC

Downstream 100 bases:

>100_bases
CTACCGATCCAAGTTGGACGTCATCACGGATATCTCACCGCTGCGAACCCGCCTCGTGGGCGAATCCTCCATCGCCTTCG
TGCCGACGATGGGCGGATTG

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MRITQDVFQKMRDDGEKIAIVTCYDASFAALLERAGVDILLVGDSLGNVIQGEETTLPVTLDDMIYHTLCVKRGASTAFI
MTDMPFGTSQVSPEETFRNAAELMAAGANMVKIEGGSVMAETVEFLTQRGIPVCAHIGLTPQSVNQLGGYKVQGKTDYEA
EQLLEDAVALEQAGAGMLLMEVVPAALAAQVTRTLSIPTIGIGAGKDCSAQVLVLYDMLGIYSGKKARFMKNFLTGAGSI
EEAVQNYTRAVKTGEFPGPEHTF

Sequences:

>Translated_263_residues
MRITQDVFQKMRDDGEKIAIVTCYDASFAALLERAGVDILLVGDSLGNVIQGEETTLPVTLDDMIYHTLCVKRGASTAFI
MTDMPFGTSQVSPEETFRNAAELMAAGANMVKIEGGSVMAETVEFLTQRGIPVCAHIGLTPQSVNQLGGYKVQGKTDYEA
EQLLEDAVALEQAGAGMLLMEVVPAALAAQVTRTLSIPTIGIGAGKDCSAQVLVLYDMLGIYSGKKARFMKNFLTGAGSI
EEAVQNYTRAVKTGEFPGPEHTF
>Mature_263_residues
MRITQDVFQKMRDDGEKIAIVTCYDASFAALLERAGVDILLVGDSLGNVIQGEETTLPVTLDDMIYHTLCVKRGASTAFI
MTDMPFGTSQVSPEETFRNAAELMAAGANMVKIEGGSVMAETVEFLTQRGIPVCAHIGLTPQSVNQLGGYKVQGKTDYEA
EQLLEDAVALEQAGAGMLLMEVVPAALAAQVTRTLSIPTIGIGAGKDCSAQVLVLYDMLGIYSGKKARFMKNFLTGAGSI
EEAVQNYTRAVKTGEFPGPEHTF

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=260, Percent_Identity=50.7692307692308, Blast_Score=278, Evalue=3e-76,
Organism=Saccharomyces cerevisiae, GI6319653, Length=273, Percent_Identity=36.6300366300366, Blast_Score=172, Evalue=5e-44,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_NITMU (Q2YAN9)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411574.1
- ProteinModelPortal:   Q2YAN9
- SMR:   Q2YAN9
- STRING:   Q2YAN9
- GeneID:   3785921
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0879
- eggNOG:   COG0413
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- PhylomeDB:   Q2YAN9
- ProtClustDB:   PRK00311
- BioCyc:   NMUL323848:NMUL_A0879-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 28127; Mature: 28127

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: ACT_SITE 181-181 BINDING 83-83 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRITQDVFQKMRDDGEKIAIVTCYDASFAALLERAGVDILLVGDSLGNVIQGEETTLPVT
CCCHHHHHHHHHCCCCEEEEEEEECHHHHHHHHHCCCEEEEECCCCCCCCCCCCCEECEE
LDDMIYHTLCVKRGASTAFIMTDMPFGTSQVSPEETFRNAAELMAAGANMVKIEGGSVMA
HHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHH
ETVEFLTQRGIPVCAHIGLTPQSVNQLGGYKVQGKTDYEAEQLLEDAVALEQAGAGMLLM
HHHHHHHHCCCCEEEECCCCHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHCCCCCHHH
EVVPAALAAQVTRTLSIPTIGIGAGKDCSAQVLVLYDMLGIYSGKKARFMKNFLTGAGSI
HHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHH
EEAVQNYTRAVKTGEFPGPEHTF
HHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MRITQDVFQKMRDDGEKIAIVTCYDASFAALLERAGVDILLVGDSLGNVIQGEETTLPVT
CCCHHHHHHHHHCCCCEEEEEEEECHHHHHHHHHCCCEEEEECCCCCCCCCCCCCEECEE
LDDMIYHTLCVKRGASTAFIMTDMPFGTSQVSPEETFRNAAELMAAGANMVKIEGGSVMA
HHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHH
ETVEFLTQRGIPVCAHIGLTPQSVNQLGGYKVQGKTDYEAEQLLEDAVALEQAGAGMLLM
HHHHHHHHCCCCEEEECCCCHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHCCCCCHHH
EVVPAALAAQVTRTLSIPTIGIGAGKDCSAQVLVLYDMLGIYSGKKARFMKNFLTGAGSI
HHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHH
EEAVQNYTRAVKTGEFPGPEHTF
HHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA