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The map label for this gene is mdh

Identifier: 82701993

GI number: 82701993

Start: 982671

End: 983654

Strand: Direct

Name: mdh

Synonym: Nmul_A0864

Alternate gene names: 82701993

Gene position: 982671-983654 (Clockwise)

Preceding gene: 82701983

Following gene: 82701994

Centisome position: 30.86

GC content: 55.89

Gene sequence:

>984_bases
ATGAAGACTCCCATCCGTGTCGCCGTAACCGGCGCCGCCGGCCAGATAGCTTACAGTCTCTTGTTTCGTATCGCAGCGGG
CGACATGCTTGGCGAGGATCAGCCTGTCATTCTGCAATTGCTGGATATTCCTCAATCGTTGCCTTCCTTGAAAGGCGTTG
TCATGGAACTGGACGACTGTGCCTTTCCATTACTGAGGGACATCACCATTACCGACGACCCCAAAACGGCTTTTCGCGAC
ATAAATATCGCCATGCTGGTTGGTGCCCGTCCGCGCACCAAGGGTATGGAACGCAAGGATCTGCTGGAAGCAAACGGTAC
GATATTCCGTGCGCAGGGGAAGGCGCTCGACGAGGTTGCCGGGCGCGATGTGAAAGTCCTGGTGGTGGGGAATCCCGCCA
ATACCAATGCCTATATCACGATGAAAAATGCGCCGAGTCTGAAGCCGACCAGCTTCTCGTCGATGATGCGACTGGACCAT
AACCGCGCAGTGTTCCAGCTTGCCGTCAAGGTCGGCCAGCCGGTTTCCAGTGTGAGAAAGATGATCGTATGGGGGAATCA
CTCCTCAGCCCAATATCCCGATTTGAGCCATGCCGAGGTGGACGGCCATAATGCCGCTGACCTCGTCAATGACATGGCAT
GGATAGAAACCGGTTTCATTCCCGTAATACAGAAGCGCGGCATGGAGGTGATCGAGGCGCGTGGTTCTTCGAGTGCGGCC
AGCGCGGCCAACGCCGCAATCTGCCACATGCGCGACTGGGTTTCGGGCACTCCCGAAGGTGACTGGGTGTCCATGGGTAT
TCCTTCGGATGGAAGCTACGGCATCCCGGAAGGAGTTATCTATGGTTACCCCGTGACCTGTCAGGGCGGTGAATACAAGA
TCGTGCCCGACCTGGAAATAAGCGAGTTCAGCAGGATGAAGATGCAGGCGAGCTACCGGGAATTAATGGGAGAGCGCGAG
TCGATAAAGCATCTGCTTGGATAA

Upstream 100 bases:

>100_bases
TTCACTTTTCCGCTTCTCTGTCTGGTCCGGTTCGTTACAGCCTGAAGGACGCAATGGAAAATTACTACTCGGTAGTATAT
CTACAGGAGCATAAGAAAAA

Downstream 100 bases:

>100_bases
GCCGCACTCCCGTCTTCGTTTCTCCTTTCATGCCTGCCTCTGCCGGTTCCCGTTTTCGCGCAGCGCTTGACGCGGAAATG
CCCCTGCAGATCGCAGGCTG

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MKTPIRVAVTGAAGQIAYSLLFRIAAGDMLGEDQPVILQLLDIPQSLPSLKGVVMELDDCAFPLLRDITITDDPKTAFRD
INIAMLVGARPRTKGMERKDLLEANGTIFRAQGKALDEVAGRDVKVLVVGNPANTNAYITMKNAPSLKPTSFSSMMRLDH
NRAVFQLAVKVGQPVSSVRKMIVWGNHSSAQYPDLSHAEVDGHNAADLVNDMAWIETGFIPVIQKRGMEVIEARGSSSAA
SAANAAICHMRDWVSGTPEGDWVSMGIPSDGSYGIPEGVIYGYPVTCQGGEYKIVPDLEISEFSRMKMQASYRELMGERE
SIKHLLG

Sequences:

>Translated_327_residues
MKTPIRVAVTGAAGQIAYSLLFRIAAGDMLGEDQPVILQLLDIPQSLPSLKGVVMELDDCAFPLLRDITITDDPKTAFRD
INIAMLVGARPRTKGMERKDLLEANGTIFRAQGKALDEVAGRDVKVLVVGNPANTNAYITMKNAPSLKPTSFSSMMRLDH
NRAVFQLAVKVGQPVSSVRKMIVWGNHSSAQYPDLSHAEVDGHNAADLVNDMAWIETGFIPVIQKRGMEVIEARGSSSAA
SAANAAICHMRDWVSGTPEGDWVSMGIPSDGSYGIPEGVIYGYPVTCQGGEYKIVPDLEISEFSRMKMQASYRELMGERE
SIKHLLG
>Mature_327_residues
MKTPIRVAVTGAAGQIAYSLLFRIAAGDMLGEDQPVILQLLDIPQSLPSLKGVVMELDDCAFPLLRDITITDDPKTAFRD
INIAMLVGARPRTKGMERKDLLEANGTIFRAQGKALDEVAGRDVKVLVVGNPANTNAYITMKNAPSLKPTSFSSMMRLDH
NRAVFQLAVKVGQPVSSVRKMIVWGNHSSAQYPDLSHAEVDGHNAADLVNDMAWIETGFIPVIQKRGMEVIEARGSSSAA
SAANAAICHMRDWVSGTPEGDWVSMGIPSDGSYGIPEGVIYGYPVTCQGGEYKIVPDLEISEFSRMKMQASYRELMGERE
SIKHLLG

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family

Homologues:

Organism=Homo sapiens, GI5174539, Length=331, Percent_Identity=51.3595166163142, Blast_Score=342, Evalue=3e-94,
Organism=Homo sapiens, GI89886456, Length=329, Percent_Identity=25.531914893617, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17561064, Length=325, Percent_Identity=52.9230769230769, Blast_Score=326, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI32566798, Length=262, Percent_Identity=53.4351145038168, Blast_Score=254, Evalue=4e-68,
Organism=Caenorhabditis elegans, GI32566800, Length=170, Percent_Identity=49.4117647058824, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24583394, Length=330, Percent_Identity=52.7272727272727, Blast_Score=341, Evalue=5e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MDH_NITMU (Q2YAQ4)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411559.1
- HSSP:   P10584
- ProteinModelPortal:   Q2YAQ4
- SMR:   Q2YAQ4
- STRING:   Q2YAQ4
- GeneID:   3784434
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0864
- eggNOG:   COG0039
- HOGENOM:   HBG289884
- OMA:   MIIWGNH
- ProtClustDB:   PRK05442
- BioCyc:   NMUL323848:NMUL_A0864-MONOMER
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_01517
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR010945
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR23382
- PIRSF:   PIRSF000102
- TIGRFAMs:   TIGR01759

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 35521; Mature: 35521

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00068 MDH

Important sites: ACT_SITE 187-187 BINDING 92-92 BINDING 98-98 BINDING 105-105 BINDING 112-112 BINDING 131-131 BINDING 162-162

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTPIRVAVTGAAGQIAYSLLFRIAAGDMLGEDQPVILQLLDIPQSLPSLKGVVMELDDC
CCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCHHHHHHHHHHEEEHHHH
AFPLLRDITITDDPKTAFRDINIAMLVGARPRTKGMERKDLLEANGTIFRAQGKALDEVA
HHHHHHCCEECCCCHHHHHCCEEEEEECCCCCCCCCCHHHHHHCCCCEEEECCCHHHHHC
GRDVKVLVVGNPANTNAYITMKNAPSLKPTSFSSMMRLDHNRAVFQLAVKVGQPVSSVRK
CCCEEEEEECCCCCCCEEEEECCCCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCHHHHCE
MIVWGNHSSAQYPDLSHAEVDGHNAADLVNDMAWIETGFIPVIQKRGMEVIEARGSSSAA
EEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCEECCCCCCCHH
SAANAAICHMRDWVSGTPEGDWVSMGIPSDGSYGIPEGVIYGYPVTCQGGEYKIVPDLEI
HHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCEEECCEEEECCCCEEEECCCCH
SEFSRMKMQASYRELMGERESIKHLLG
HHHHHHHHHHHHHHHCCCHHHHHHHCC
>Mature Secondary Structure
MKTPIRVAVTGAAGQIAYSLLFRIAAGDMLGEDQPVILQLLDIPQSLPSLKGVVMELDDC
CCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCHHHHHHHHHHEEEHHHH
AFPLLRDITITDDPKTAFRDINIAMLVGARPRTKGMERKDLLEANGTIFRAQGKALDEVA
HHHHHHCCEECCCCHHHHHCCEEEEEECCCCCCCCCCHHHHHHCCCCEEEECCCHHHHHC
GRDVKVLVVGNPANTNAYITMKNAPSLKPTSFSSMMRLDHNRAVFQLAVKVGQPVSSVRK
CCCEEEEEECCCCCCCEEEEECCCCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCHHHHCE
MIVWGNHSSAQYPDLSHAEVDGHNAADLVNDMAWIETGFIPVIQKRGMEVIEARGSSSAA
EEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCEECCCCCCCHH
SAANAAICHMRDWVSGTPEGDWVSMGIPSDGSYGIPEGVIYGYPVTCQGGEYKIVPDLEI
HHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCEEECCEEEECCCCEEEECCCCH
SEFSRMKMQASYRELMGERESIKHLLG
HHHHHHHHHHHHHHHCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA