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The map label for this gene is sucB [H]

Identifier: 82701985

GI number: 82701985

Start: 973164

End: 974549

Strand: Reverse

Name: sucB [H]

Synonym: Nmul_A0856

Alternate gene names: 82701985

Gene position: 974549-973164 (Counterclockwise)

Preceding gene: 82701986

Following gene: 82701984

Centisome position: 30.61

GC content: 57.65

Gene sequence:

>1386_bases
ATGCGCGTCGATATCAAAGTTCCCGCGTTATCCGAATCAGTGGCCCAGGCAACGCTCCTTTCCTGGCACAAAAAGGAAGG
GGAGCATGTTGAGCGTGACGAAAATCTGATCGACGTCGAAACTGATAAAGTCGTGATGGAGTTGCCAGCTCCCGCTACGG
GAACACTGGCGAAAATCATCAAGGGAGACGGAGCAACGGTGACTGGCGGGGAAGTCATCGCCATGATAGATACCGAAGCA
GGTGCGACAAAGGATAACACTCCGGCAGCTTCAACCGCAGCCCCAAGCAAGTCCAAAGTAACCGAATCCGGAGCAGCGCC
TTCTGCCGCCGAAGCCAGGCCGGCCAAAAAAGAGGCAGAAGCTGCTCCGCCTTCCGCAACTGCCCCGGCTTCCACAACCG
CCGCGGAAGTTCCGGGAATGATGCCTGCCGCGCAAAAACTGGCTGCGCAGGAAAATCTGGCGCCGGAAGAAATTCGCGCA
CTCAAAGGCAGCGGTCGCGATGGTCGCATCACCAAGGAAGACGTGGCGGCTTATGTAGAACAAAAACGCTCGACAGCCAA
TATCGCCCCCGCGCCTTCTCCTGCGGTTCCGCAAGTTCCGGCAGCTCCCCCTCCCTCCCCTGCCCCGCCCGTTTCACCCG
CCCCTGAGTCCGCGCCCCGGATGGCTGAAGACAAGGCTGAGGGAAAACGCAGCGAAAAAAGAGTACCCATGTCGCGCCTG
CGTGCGCGCATCGCGGAACGTCTGGTGCAGTCGCAATCCACCGCCGCCATCCTTACCACATTCAACGAAGTCAACATGCA
GGCGATCATGGATTTGCGCGCCCGCTACAAGGACAAATTTGAAAAGGAGCATGGCGTCAAACTCGGTCTCACCTCATTCT
TTGTCAAAGCGGCGGTGGCGGCGCTCAAAAAATTTCCCATCGTCAACGCTTCAGTGGACGGCAACGATATCGTCTATCAC
GAATACTATGATATCGGCATTGCGGTGAGCAGTGCACGCGGGCTGGTCGTCCCCATTATTCGCAATGCGGATTCCTTGTC
CCAGGCGGAAATTGAAAGGCAGGTCACCGATTTTGGCCGCCGCGCGCAGGACGGCAAACTGACCATCGAGGAATTGACCG
GCGGCACTTTCTCGATCACCAATGGCGGCGTCTTCGGTTCGATGCTTTCCACCCCCATCATCAATCCCCCGCAGAGCGCC
ATTCTTGGCATTCACGCAACCAAGGAGCGCCCGGTGGTCGAAAACGGCCAAATCGTGATTCGTCCGATATGCTATCTGGC
GCTATCCTATGACCATCGCATCATAGACGGGCGGGAAGCCGTGCTTTCCCTGGTAGCGATGAAGGAAGCGCTCGAATATC
CCATGAGCCCCCTGCTTGAGAGCTGA

Upstream 100 bases:

>100_bases
CGGCGGCGGGCTATCTGGTGAAACACAACGAACAGCAGAACGAGTTGATCGTATCTGCGTTTCGCGAAAGAATCTGAATA
CTTGGAGGCAGAGGAAGAAT

Downstream 100 bases:

>100_bases
GATTGCGCGGGTACAGGTCTGCGCAGCCGGCGTCTTTCCGTTTTACATTCCTTTTGCATTCCTTGCTTAAACAATAAAGA
GAAAATATGTCTCAATCTTT

Product: 2-oxoglutarate dehydrogenase, E2 component, dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MRVDIKVPALSESVAQATLLSWHKKEGEHVERDENLIDVETDKVVMELPAPATGTLAKIIKGDGATVTGGEVIAMIDTEA
GATKDNTPAASTAAPSKSKVTESGAAPSAAEARPAKKEAEAAPPSATAPASTTAAEVPGMMPAAQKLAAQENLAPEEIRA
LKGSGRDGRITKEDVAAYVEQKRSTANIAPAPSPAVPQVPAAPPPSPAPPVSPAPESAPRMAEDKAEGKRSEKRVPMSRL
RARIAERLVQSQSTAAILTTFNEVNMQAIMDLRARYKDKFEKEHGVKLGLTSFFVKAAVAALKKFPIVNASVDGNDIVYH
EYYDIGIAVSSARGLVVPIIRNADSLSQAEIERQVTDFGRRAQDGKLTIEELTGGTFSITNGGVFGSMLSTPIINPPQSA
ILGIHATKERPVVENGQIVIRPICYLALSYDHRIIDGREAVLSLVAMKEALEYPMSPLLES

Sequences:

>Translated_461_residues
MRVDIKVPALSESVAQATLLSWHKKEGEHVERDENLIDVETDKVVMELPAPATGTLAKIIKGDGATVTGGEVIAMIDTEA
GATKDNTPAASTAAPSKSKVTESGAAPSAAEARPAKKEAEAAPPSATAPASTTAAEVPGMMPAAQKLAAQENLAPEEIRA
LKGSGRDGRITKEDVAAYVEQKRSTANIAPAPSPAVPQVPAAPPPSPAPPVSPAPESAPRMAEDKAEGKRSEKRVPMSRL
RARIAERLVQSQSTAAILTTFNEVNMQAIMDLRARYKDKFEKEHGVKLGLTSFFVKAAVAALKKFPIVNASVDGNDIVYH
EYYDIGIAVSSARGLVVPIIRNADSLSQAEIERQVTDFGRRAQDGKLTIEELTGGTFSITNGGVFGSMLSTPIINPPQSA
ILGIHATKERPVVENGQIVIRPICYLALSYDHRIIDGREAVLSLVAMKEALEYPMSPLLES
>Mature_461_residues
MRVDIKVPALSESVAQATLLSWHKKEGEHVERDENLIDVETDKVVMELPAPATGTLAKIIKGDGATVTGGEVIAMIDTEA
GATKDNTPAASTAAPSKSKVTESGAAPSAAEARPAKKEAEAAPPSATAPASTTAAEVPGMMPAAQKLAAQENLAPEEIRA
LKGSGRDGRITKEDVAAYVEQKRSTANIAPAPSPAVPQVPAAPPPSPAPPVSPAPESAPRMAEDKAEGKRSEKRVPMSRL
RARIAERLVQSQSTAAILTTFNEVNMQAIMDLRARYKDKFEKEHGVKLGLTSFFVKAAVAALKKFPIVNASVDGNDIVYH
EYYDIGIAVSSARGLVVPIIRNADSLSQAEIERQVTDFGRRAQDGKLTIEELTGGTFSITNGGVFGSMLSTPIINPPQSA
ILGIHATKERPVVENGQIVIRPICYLALSYDHRIIDGREAVLSLVAMKEALEYPMSPLLES

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=249, Percent_Identity=52.6104417670683, Blast_Score=273, Evalue=3e-73,
Organism=Homo sapiens, GI203098753, Length=472, Percent_Identity=31.1440677966102, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI203098816, Length=472, Percent_Identity=31.1440677966102, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI31711992, Length=468, Percent_Identity=29.0598290598291, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI110671329, Length=466, Percent_Identity=28.1115879828326, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI260898739, Length=164, Percent_Identity=35.9756097560976, Blast_Score=97, Evalue=4e-20,
Organism=Escherichia coli, GI1786946, Length=458, Percent_Identity=46.0698689956332, Blast_Score=400, Evalue=1e-113,
Organism=Escherichia coli, GI1786305, Length=419, Percent_Identity=30.3102625298329, Blast_Score=146, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI25146366, Length=227, Percent_Identity=53.7444933920705, Blast_Score=265, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI17560088, Length=470, Percent_Identity=29.5744680851064, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17537937, Length=464, Percent_Identity=26.0775862068966, Blast_Score=141, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI17538894, Length=326, Percent_Identity=31.2883435582822, Blast_Score=115, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6320352, Length=455, Percent_Identity=39.7802197802198, Blast_Score=311, Evalue=1e-85,
Organism=Saccharomyces cerevisiae, GI6324258, Length=481, Percent_Identity=27.8586278586279, Blast_Score=142, Evalue=9e-35,
Organism=Drosophila melanogaster, GI24645909, Length=252, Percent_Identity=44.047619047619, Blast_Score=217, Evalue=1e-56,
Organism=Drosophila melanogaster, GI18859875, Length=466, Percent_Identity=25.5364806866953, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI20129315, Length=228, Percent_Identity=32.0175438596491, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24582497, Length=228, Percent_Identity=32.0175438596491, Blast_Score=119, Evalue=5e-27,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 48856; Mature: 48856

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVDIKVPALSESVAQATLLSWHKKEGEHVERDENLIDVETDKVVMELPAPATGTLAKII
CEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCEEEEECCCCCCHHHHHHH
KGDGATVTGGEVIAMIDTEAGATKDNTPAASTAAPSKSKVTESGAAPSAAEARPAKKEAE
CCCCCEEECCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHCCCCHHHHC
AAPPSATAPASTTAAEVPGMMPAAQKLAAQENLAPEEIRALKGSGRDGRITKEDVAAYVE
CCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHH
QKRSTANIAPAPSPAVPQVPAAPPPSPAPPVSPAPESAPRMAEDKAEGKRSEKRVPMSRL
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHCCCHHHH
RARIAERLVQSQSTAAILTTFNEVNMQAIMDLRARYKDKFEKEHGVKLGLTSFFVKAAVA
HHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHH
ALKKFPIVNASVDGNDIVYHEYYDIGIAVSSARGLVVPIIRNADSLSQAEIERQVTDFGR
HHHHCCCEECCCCCCEEEEEEEEEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHC
RAQDGKLTIEELTGGTFSITNGGVFGSMLSTPIINPPQSAILGIHATKERPVVENGQIVI
CCCCCCEEEEECCCCEEEECCCCHHHHHHHCCCCCCCHHHEEEEECCCCCCCCCCCCEEE
RPICYLALSYDHRIIDGREAVLSLVAMKEALEYPMSPLLES
EEHHHHHHCCCCEEECHHHHHHHHHHHHHHHHCCHHHHCCC
>Mature Secondary Structure
MRVDIKVPALSESVAQATLLSWHKKEGEHVERDENLIDVETDKVVMELPAPATGTLAKII
CEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCEEEEECCCCCCHHHHHHH
KGDGATVTGGEVIAMIDTEAGATKDNTPAASTAAPSKSKVTESGAAPSAAEARPAKKEAE
CCCCCEEECCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHCCCCHHHHC
AAPPSATAPASTTAAEVPGMMPAAQKLAAQENLAPEEIRALKGSGRDGRITKEDVAAYVE
CCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHH
QKRSTANIAPAPSPAVPQVPAAPPPSPAPPVSPAPESAPRMAEDKAEGKRSEKRVPMSRL
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHCCCHHHH
RARIAERLVQSQSTAAILTTFNEVNMQAIMDLRARYKDKFEKEHGVKLGLTSFFVKAAVA
HHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHH
ALKKFPIVNASVDGNDIVYHEYYDIGIAVSSARGLVVPIIRNADSLSQAEIERQVTDFGR
HHHHCCCEECCCCCCEEEEEEEEEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHC
RAQDGKLTIEELTGGTFSITNGGVFGSMLSTPIINPPQSAILGIHATKERPVVENGQIVI
CCCCCCEEEEECCCCEEEECCCCHHHHHHHCCCCCCCHHHEEEEECCCCCCCCCCCCEEE
RPICYLALSYDHRIIDGREAVLSLVAMKEALEYPMSPLLES
EEHHHHHHCCCCEEECHHHHHHHHHHHHHHHHCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]