Click here to switch to the map view.

The map label for this gene is odhL [H]

Identifier: 82701984

GI number: 82701984

Start: 971626

End: 973077

Strand: Reverse

Name: odhL [H]

Synonym: Nmul_A0855

Alternate gene names: 82701984

Gene position: 973077-971626 (Counterclockwise)

Preceding gene: 82701985

Following gene: 82701974

Centisome position: 30.56

GC content: 54.55

Gene sequence:

>1452_bases
ATGTCTCAATCTTTTGATGTGGCTGTAATCGGCGCCGGTCCGGGTGGTTATGTGGCGGCAATTCGTTGTGCGCAACTGGG
CCTTAAAACCGTTTGTATCGATGAATGGAAAAATCCGCAGGACAAACCCAGCCTCGGCGGAACCTGTCTGAATGTCGGCT
GCATCCCCTCAAAGGCATTGCTGGAATCCTCGGAGAATTACGAGCGCGCCGCCCGCAAATTCTCTGCCCATGGCATCAAA
GTGGAGGGTCTGTCCATAGACATTCCGGCGATGATAGGCCGCAAGGACAAGATCGTAGCAAACTTCACCGGCGGCGTTGC
CATGCTGCTGAAGAAGAACAAAGTCGCATCCATGCATGGGCGGGCGACGCTGCTCAAGCGCGACAGGGATAACGAAGCCG
ATCGTGAACTCTGGCAGATTGAAATCAGGAATGGAGACAAGGTGGAGACTGTAGGAGCCGAGCATGTCATCATCGCTACA
GGTTCAGTGCCTCGTCAATTAGACGCCGCGCCCGTGGACAATGAACGGATACTGGACAATGCAGGGGCGCTGGCGTTGAC
CGAAACGCCGAAACGGCTGGGAGTGATCGGCGGAGGAGTCATCGGCCTGGAAATGGGGAGTGTATGGCGCAGGCTGGGTG
CGGAGGTGACTATCCTGGAAGCCTTGCCCGGGTTTCTCATGAGCGCGGACGAACAGGTGGCTAAGGAAGCGCGAAAAATC
TTCAGCCGGGAACTGGGTCTGGTTATAAATACCGGCGTCAAAATAAGCGGCATTACCTCGGGACAGGATAACGTCACGGT
GGAGTATTCCGATGTCGACGGAAACCCGCAAAAACTCGAGGTGGATAAACTGATTGTTGCCGTCGGGCGAGTGCCTAATA
CTACCGGACTGGGTGCAGAAAACGTAGGATTAAAGTTGGACGAGCGCGAGCGTATCGAGGTGGATGTCCACTGCCGCACC
AATCTGCCTAATATATATGCGGTGGGAGATGTGGTGCGCGGTCCCATGCTGGCACATAAAGCTTCCGAAGAAGGTGTGGC
GGTTGCAGAAATGATTGCAGGACAGGCTGGTCATCTAAATCTTGACGCAATCCCCTGGGTTATCTATACCTCACCCGAAA
TCGCCTGGGTGGGCAAGACTGAACAGGAGTTGAAAGCAGCCGGGGTGGAATATAAAGCCGGACAATTCCCTTTCATGGCA
AATGGGCGTGCACGCGCACTGGGAGAAACCGGTGGATTCGTCAAAGTGCTTGCGGATGCAGATACTGATCGTATTCTTGG
TATTCACATGATTGGTCCCTACGTTTCCGAATTGATTGCCGAGGCTGTGGTGGCAATGGAATTCGCAGCGAGCAGCGAAG
ATATCGCGCGCATCGTCCACGCACACCCTTCTCTGTCAGAAGTCGTGCATGAGGCCGCACTGGCGGTGGATAAGCGGGCG
ATACATATTTAA

Upstream 100 bases:

>100_bases
TGCTTGAGAGCTGAGATTGCGCGGGTACAGGTCTGCGCAGCCGGCGTCTTTCCGTTTTACATTCCTTTTGCATTCCTTGC
TTAAACAATAAAGAGAAAAT

Downstream 100 bases:

>100_bases
GAGGACGGAGCACAGTCCCAAAGAAAGATCGGGAGGTACTTCAAGGCCACGGAAGATGTTCAACCGTGGCCTTCAGGTAT
TCCCTGATTAATTTGGCGAA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MSQSFDVAVIGAGPGGYVAAIRCAQLGLKTVCIDEWKNPQDKPSLGGTCLNVGCIPSKALLESSENYERAARKFSAHGIK
VEGLSIDIPAMIGRKDKIVANFTGGVAMLLKKNKVASMHGRATLLKRDRDNEADRELWQIEIRNGDKVETVGAEHVIIAT
GSVPRQLDAAPVDNERILDNAGALALTETPKRLGVIGGGVIGLEMGSVWRRLGAEVTILEALPGFLMSADEQVAKEARKI
FSRELGLVINTGVKISGITSGQDNVTVEYSDVDGNPQKLEVDKLIVAVGRVPNTTGLGAENVGLKLDERERIEVDVHCRT
NLPNIYAVGDVVRGPMLAHKASEEGVAVAEMIAGQAGHLNLDAIPWVIYTSPEIAWVGKTEQELKAAGVEYKAGQFPFMA
NGRARALGETGGFVKVLADADTDRILGIHMIGPYVSELIAEAVVAMEFAASSEDIARIVHAHPSLSEVVHEAALAVDKRA
IHI

Sequences:

>Translated_483_residues
MSQSFDVAVIGAGPGGYVAAIRCAQLGLKTVCIDEWKNPQDKPSLGGTCLNVGCIPSKALLESSENYERAARKFSAHGIK
VEGLSIDIPAMIGRKDKIVANFTGGVAMLLKKNKVASMHGRATLLKRDRDNEADRELWQIEIRNGDKVETVGAEHVIIAT
GSVPRQLDAAPVDNERILDNAGALALTETPKRLGVIGGGVIGLEMGSVWRRLGAEVTILEALPGFLMSADEQVAKEARKI
FSRELGLVINTGVKISGITSGQDNVTVEYSDVDGNPQKLEVDKLIVAVGRVPNTTGLGAENVGLKLDERERIEVDVHCRT
NLPNIYAVGDVVRGPMLAHKASEEGVAVAEMIAGQAGHLNLDAIPWVIYTSPEIAWVGKTEQELKAAGVEYKAGQFPFMA
NGRARALGETGGFVKVLADADTDRILGIHMIGPYVSELIAEAVVAMEFAASSEDIARIVHAHPSLSEVVHEAALAVDKRA
IHI
>Mature_482_residues
SQSFDVAVIGAGPGGYVAAIRCAQLGLKTVCIDEWKNPQDKPSLGGTCLNVGCIPSKALLESSENYERAARKFSAHGIKV
EGLSIDIPAMIGRKDKIVANFTGGVAMLLKKNKVASMHGRATLLKRDRDNEADRELWQIEIRNGDKVETVGAEHVIIATG
SVPRQLDAAPVDNERILDNAGALALTETPKRLGVIGGGVIGLEMGSVWRRLGAEVTILEALPGFLMSADEQVAKEARKIF
SRELGLVINTGVKISGITSGQDNVTVEYSDVDGNPQKLEVDKLIVAVGRVPNTTGLGAENVGLKLDERERIEVDVHCRTN
LPNIYAVGDVVRGPMLAHKASEEGVAVAEMIAGQAGHLNLDAIPWVIYTSPEIAWVGKTEQELKAAGVEYKAGQFPFMAN
GRARALGETGGFVKVLADADTDRILGIHMIGPYVSELIAEAVVAMEFAASSEDIARIVHAHPSLSEVVHEAALAVDKRAI
HI

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=478, Percent_Identity=44.3514644351464, Blast_Score=397, Evalue=1e-110,
Organism=Homo sapiens, GI50301238, Length=480, Percent_Identity=27.9166666666667, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI22035672, Length=482, Percent_Identity=27.8008298755187, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI33519430, Length=486, Percent_Identity=24.2798353909465, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI33519428, Length=486, Percent_Identity=24.2798353909465, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI33519426, Length=486, Percent_Identity=24.2798353909465, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI148277065, Length=486, Percent_Identity=24.2798353909465, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI148277071, Length=486, Percent_Identity=24.2798353909465, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI291045266, Length=457, Percent_Identity=23.1947483588621, Blast_Score=96, Evalue=8e-20,
Organism=Escherichia coli, GI1786307, Length=469, Percent_Identity=39.8720682302772, Blast_Score=321, Evalue=7e-89,
Organism=Escherichia coli, GI87082354, Length=484, Percent_Identity=30.1652892561983, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI87081717, Length=475, Percent_Identity=27.1578947368421, Blast_Score=145, Evalue=7e-36,
Organism=Escherichia coli, GI1789915, Length=472, Percent_Identity=27.5423728813559, Blast_Score=135, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI32565766, Length=478, Percent_Identity=46.8619246861925, Blast_Score=421, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17557007, Length=498, Percent_Identity=27.3092369477912, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI71983419, Length=342, Percent_Identity=27.7777777777778, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71983429, Length=342, Percent_Identity=27.7777777777778, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71982272, Length=495, Percent_Identity=23.2323232323232, Blast_Score=92, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17559934, Length=211, Percent_Identity=31.2796208530806, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6321091, Length=493, Percent_Identity=45.6389452332657, Blast_Score=395, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6325240, Length=484, Percent_Identity=32.4380165289256, Blast_Score=228, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6325166, Length=483, Percent_Identity=27.7432712215321, Blast_Score=153, Evalue=5e-38,
Organism=Drosophila melanogaster, GI21358499, Length=474, Percent_Identity=46.8354430379747, Blast_Score=420, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24640549, Length=500, Percent_Identity=27.2, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24640553, Length=500, Percent_Identity=27.2, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24640551, Length=500, Percent_Identity=27.2, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI17737741, Length=496, Percent_Identity=26.4112903225806, Blast_Score=112, Evalue=5e-25,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 51517; Mature: 51386

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSFDVAVIGAGPGGYVAAIRCAQLGLKTVCIDEWKNPQDKPSLGGTCLNVGCIPSKAL
CCCCCCEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCEEEEECCCCCHHH
LESSENYERAARKFSAHGIKVEGLSIDIPAMIGRKDKIVANFTGGVAMLLKKNKVASMHG
HCCCCHHHHHHHHHHCCCEEEECEEEECHHHCCCCCEEEEEECCCEEEEEECCCHHHCCC
RATLLKRDRDNEADRELWQIEIRNGDKVETVGAEHVIIATGSVPRQLDAAPVDNERILDN
CEEEEECCCCCCCCCEEEEEEECCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCHHHCC
AGALALTETPKRLGVIGGGVIGLEMGSVWRRLGAEVTILEALPGFLMSADEQVAKEARKI
CCCEEEECCCHHHEEECCCEEEEEHHHHHHHHCCCEEEHHHCCHHHHCCHHHHHHHHHHH
FSRELGLVINTGVKISGITSGQDNVTVEYSDVDGNPQKLEVDKLIVAVGRVPNTTGLGAE
HHHCCCEEEECCEEEEEECCCCCCEEEEEECCCCCCCEEEHHHEEEEECCCCCCCCCCCC
NVGLKLDERERIEVDVHCRTNLPNIYAVGDVVRGPMLAHKASEEGVAVAEMIAGQAGHLN
CCCEEECCCCCEEEEEEEECCCCCEEEEHHHHCCCHHHHCCCCCCCHHHHHHCCCCCCCC
LDAIPWVIYTSPEIAWVGKTEQELKAAGVEYKAGQFPFMANGRARALGETGGFVKVLADA
CCCCCEEEEECCCEEEECCCHHHHHHCCCCCCCCCCCEEECCCCEEECCCCCEEEEEECC
DTDRILGIHMIGPYVSELIAEAVVAMEFAASSEDIARIVHAHPSLSEVVHEAALAVDKRA
CCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCE
IHI
ECC
>Mature Secondary Structure 
SQSFDVAVIGAGPGGYVAAIRCAQLGLKTVCIDEWKNPQDKPSLGGTCLNVGCIPSKAL
CCCCCEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCEEEEECCCCCHHH
LESSENYERAARKFSAHGIKVEGLSIDIPAMIGRKDKIVANFTGGVAMLLKKNKVASMHG
HCCCCHHHHHHHHHHCCCEEEECEEEECHHHCCCCCEEEEEECCCEEEEEECCCHHHCCC
RATLLKRDRDNEADRELWQIEIRNGDKVETVGAEHVIIATGSVPRQLDAAPVDNERILDN
CEEEEECCCCCCCCCEEEEEEECCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCHHHCC
AGALALTETPKRLGVIGGGVIGLEMGSVWRRLGAEVTILEALPGFLMSADEQVAKEARKI
CCCEEEECCCHHHEEECCCEEEEEHHHHHHHHCCCEEEHHHCCHHHHCCHHHHHHHHHHH
FSRELGLVINTGVKISGITSGQDNVTVEYSDVDGNPQKLEVDKLIVAVGRVPNTTGLGAE
HHHCCCEEEECCEEEEEECCCCCCEEEEEECCCCCCCEEEHHHEEEEECCCCCCCCCCCC
NVGLKLDERERIEVDVHCRTNLPNIYAVGDVVRGPMLAHKASEEGVAVAEMIAGQAGHLN
CCCEEECCCCCEEEEEEEECCCCCEEEEHHHHCCCHHHHCCCCCCCHHHHHHCCCCCCCC
LDAIPWVIYTSPEIAWVGKTEQELKAAGVEYKAGQFPFMANGRARALGETGGFVKVLADA
CCCCCEEEEECCCEEEECCCHHHHHHCCCCCCCCCCCEEECCCCEEECCCCCEEEEEECC
DTDRILGIHMIGPYVSELIAEAVVAMEFAASSEDIARIVHAHPSLSEVVHEAALAVDKRA
CCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCE
IHI
ECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]