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The map label for this gene is hipO [H]

Identifier: 82701982

GI number: 82701982

Start: 969465

End: 970760

Strand: Direct

Name: hipO [H]

Synonym: Nmul_A0853

Alternate gene names: 82701982

Gene position: 969465-970760 (Clockwise)

Preceding gene: 82701981

Following gene: 82701983

Centisome position: 30.45

GC content: 60.42

Gene sequence:

>1296_bases
ATGAACACGCAACCAGCGAGCTCTCTTGGAAAGGTACTAGCGGGGCTTCAGAACCTGCTGCCGGATCTGGAGGCGTTATA
TACCGACGTGCATGCCCATCCGGAGCTGTCGATGCAGGAATCGCGTACCGCAAGCCTAGTCGCGGAACGACTTCGCGCGG
CTGGGTATGACGTGACGACGAGGGTTGGGAAGACGGGTGTCGTGGGATTGCTGCGCAACGGCGACGGGCCGACCGTCATG
CTGCGTGCCGACATGGATGCACTACCCATCGAGGAAATGACCGGGCTTCCCTATGCCAGCAAGGTCAAGGCCACGAATCA
CGAGGGTAAAACGGTCCCGGTCATGCACGCTTGCGGCCATGACATGCACGTTGCCTGGCTCGTCGGTGCGACCACGCTGC
TCGCGCAGGCGCGTAATACATGGGGCGGCACGTTGATGGCAGTCTTCCAGCCGGCTGAAGAGACTGCGGAAGGCGCCCAA
GCCATGATCGACGATGGACTGTTCAACCGTTTTCCAATGCCGGATGTCGTGCTTGGCCAACACGTCATGGTGGGGCCGGC
GGGCAATATTGGCGGCCGTGCCGGATCCATCACTTCCGCTGCCGACAGCCTGCAGATCCGCCTGTTTGGGCGTGGGGCGC
ACGGATCAATGCCGCAGGCAAGCATCGATCCGGTTGTCATGGCTGCCGCGACTGTAATGCGCCTGCAGACCATTGTCTCG
CGTGAACTTGCTGCTGCCGAGGCCGCTGTCGTTACCATTGGCGCGTTGCAGGCGGGCACCAAGGAAAATGTGATACCCGA
CGAGGCGGTCATCAAGCTGAATGTACGCACCTTTGATGCGGATGTGCGCAAGCGTGTACTTGCCGCCATCGAGCGTATCG
CCAATGCAGAGGCTGCAGCTTCGGGAGCCCCCCGGCCGCCCGAGATTACGACGCTGGAACACTACCCTCTAGGAGTCAAC
GATGCCGATGCAAGCGGCCGCGTCGCCGATGCTTTCCGTCAATATTTCTCAGCCGACCGCGTGCGGCAAGTCGATGCGGC
GTCGGCGAGCGAGGATTTCGGGTTGTTCGGAACCGAGTGGGGTGTCCCTTCCGTGTTCTGGTTCGTCGGAGGTACCGATC
CCGACCTTTACGCGAAAGCCAAGGCCGCAGGTGAAATCAACAAGATTCCAACGAACCACAGTCCATACTTTGCACCAGTA
ATGCATCCCACTCTGGAAACTGGCGTGGAAACGATGGTCATTGGCGCCCTGGCTTGGCTTCAGCATGAGTCGCAGCACCA
GGAGCTGAGACCATAA

Upstream 100 bases:

>100_bases
TTGATTGATCTGATCGAGCGTATAGAATTTCACCAGTTTGTCGAAGGGGATTTTTTAAGACTTCTGCGAGTCTTGCAGCA
ATTTAACGAAGGAATTCAGC

Downstream 100 bases:

>100_bases
GGTGTTTCCTCCCGCTGCTTTTGACGTGGATGCTGCAATGAAGACCCTCGTGCTGGCGCTCGATCTTGTCGGCACCTTCG
TTTTCGCTTTGAGCGGAGCG

Product: peptidase M20D, amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MNTQPASSLGKVLAGLQNLLPDLEALYTDVHAHPELSMQESRTASLVAERLRAAGYDVTTRVGKTGVVGLLRNGDGPTVM
LRADMDALPIEEMTGLPYASKVKATNHEGKTVPVMHACGHDMHVAWLVGATTLLAQARNTWGGTLMAVFQPAEETAEGAQ
AMIDDGLFNRFPMPDVVLGQHVMVGPAGNIGGRAGSITSAADSLQIRLFGRGAHGSMPQASIDPVVMAAATVMRLQTIVS
RELAAAEAAVVTIGALQAGTKENVIPDEAVIKLNVRTFDADVRKRVLAAIERIANAEAAASGAPRPPEITTLEHYPLGVN
DADASGRVADAFRQYFSADRVRQVDAASASEDFGLFGTEWGVPSVFWFVGGTDPDLYAKAKAAGEINKIPTNHSPYFAPV
MHPTLETGVETMVIGALAWLQHESQHQELRP

Sequences:

>Translated_431_residues
MNTQPASSLGKVLAGLQNLLPDLEALYTDVHAHPELSMQESRTASLVAERLRAAGYDVTTRVGKTGVVGLLRNGDGPTVM
LRADMDALPIEEMTGLPYASKVKATNHEGKTVPVMHACGHDMHVAWLVGATTLLAQARNTWGGTLMAVFQPAEETAEGAQ
AMIDDGLFNRFPMPDVVLGQHVMVGPAGNIGGRAGSITSAADSLQIRLFGRGAHGSMPQASIDPVVMAAATVMRLQTIVS
RELAAAEAAVVTIGALQAGTKENVIPDEAVIKLNVRTFDADVRKRVLAAIERIANAEAAASGAPRPPEITTLEHYPLGVN
DADASGRVADAFRQYFSADRVRQVDAASASEDFGLFGTEWGVPSVFWFVGGTDPDLYAKAKAAGEINKIPTNHSPYFAPV
MHPTLETGVETMVIGALAWLQHESQHQELRP
>Mature_431_residues
MNTQPASSLGKVLAGLQNLLPDLEALYTDVHAHPELSMQESRTASLVAERLRAAGYDVTTRVGKTGVVGLLRNGDGPTVM
LRADMDALPIEEMTGLPYASKVKATNHEGKTVPVMHACGHDMHVAWLVGATTLLAQARNTWGGTLMAVFQPAEETAEGAQ
AMIDDGLFNRFPMPDVVLGQHVMVGPAGNIGGRAGSITSAADSLQIRLFGRGAHGSMPQASIDPVVMAAATVMRLQTIVS
RELAAAEAAVVTIGALQAGTKENVIPDEAVIKLNVRTFDADVRKRVLAAIERIANAEAAASGAPRPPEITTLEHYPLGVN
DADASGRVADAFRQYFSADRVRQVDAASASEDFGLFGTEWGVPSVFWFVGGTDPDLYAKAKAAGEINKIPTNHSPYFAPV
MHPTLETGVETMVIGALAWLQHESQHQELRP

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=306, Percent_Identity=29.0849673202614, Blast_Score=85, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 45752; Mature: 45752

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTQPASSLGKVLAGLQNLLPDLEALYTDVHAHPELSMQESRTASLVAERLRAAGYDVTT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHH
RVGKTGVVGLLRNGDGPTVMLRADMDALPIEEMTGLPYASKVKATNHEGKTVPVMHACGH
HCCCCCEEEEEECCCCCEEEEECCCCCCCHHHHCCCCCHHHCCCCCCCCCEEEEEHHCCC
DMHVAWLVGATTLLAQARNTWGGTLMAVFQPAEETAEGAQAMIDDGLFNRFPMPDVVLGQ
CCEEHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHCC
HVMVGPAGNIGGRAGSITSAADSLQIRLFGRGAHGSMPQASIDPVVMAAATVMRLQTIVS
EEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
RELAAAEAAVVTIGALQAGTKENVIPDEAVIKLNVRTFDADVRKRVLAAIERIANAEAAA
HHHHHHHHHEEEEEHHCCCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHCHHHHC
SGAPRPPEITTLEHYPLGVNDADASGRVADAFRQYFSADRVRQVDAASASEDFGLFGTEW
CCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
GVPSVFWFVGGTDPDLYAKAKAAGEINKIPTNHSPYFAPVMHPTLETGVETMVIGALAWL
CCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCEECHHCCCHHHHHHHHHHHHHHHHH
QHESQHQELRP
HHHHHHHCCCC
>Mature Secondary Structure
MNTQPASSLGKVLAGLQNLLPDLEALYTDVHAHPELSMQESRTASLVAERLRAAGYDVTT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHH
RVGKTGVVGLLRNGDGPTVMLRADMDALPIEEMTGLPYASKVKATNHEGKTVPVMHACGH
HCCCCCEEEEEECCCCCEEEEECCCCCCCHHHHCCCCCHHHCCCCCCCCCEEEEEHHCCC
DMHVAWLVGATTLLAQARNTWGGTLMAVFQPAEETAEGAQAMIDDGLFNRFPMPDVVLGQ
CCEEHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHCC
HVMVGPAGNIGGRAGSITSAADSLQIRLFGRGAHGSMPQASIDPVVMAAATVMRLQTIVS
EEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
RELAAAEAAVVTIGALQAGTKENVIPDEAVIKLNVRTFDADVRKRVLAAIERIANAEAAA
HHHHHHHHHEEEEEHHCCCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHCHHHHC
SGAPRPPEITTLEHYPLGVNDADASGRVADAFRQYFSADRVRQVDAASASEDFGLFGTEW
CCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
GVPSVFWFVGGTDPDLYAKAKAAGEINKIPTNHSPYFAPVMHPTLETGVETMVIGALAWL
CCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCEECHHCCCHHHHHHHHHHHHHHHHH
QHESQHQELRP
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]