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The map label for this gene is oprM [H]

Identifier: 82701981

GI number: 82701981

Start: 967032

End: 968546

Strand: Direct

Name: oprM [H]

Synonym: Nmul_A0851

Alternate gene names: 82701981

Gene position: 967032-968546 (Clockwise)

Preceding gene: 82701980

Following gene: 82701982

Centisome position: 30.37

GC content: 56.37

Gene sequence:

>1515_bases
ATGCGATCCCGGCTGACACTACTTGCATTCCTGCTTCCCCTGGTTCTTGTACTGCAAAGCTGCATGTTTGGTCCAAATTA
CCGGCGGCCAGAAATAGAAACAATGGAGAAATACCGTTTCGGAGAGAAAGTCGCGAGAGATATTGCCGATACCGCCTGGT
GGGAGCAATTCGGAGACCCCATTCTCAATGAACTGATCAGGATCGCGCTTGTTGAGAACAAGGACATACGCATCGCCACT
GCGCGTATAGAAGAATTCCAGGGCCGCTATGGCGCCACTCGCTCGGAACTGTTTCCGCAGATCGGGGGAGATGCCTCAGC
GGAGAGGTTGCGCTCGACAGCCTTCGTGTGGCCAAGAGGTCTGGGGGGCATTGACCCTTACTATAGCTATTACCAGGTAT
CCATAAATGCGAACTGGGAAATTGATATCTGGGGCCGCATCAGGCGTCTCTCGGAGGCGGCGCGCCATCAGCTGTTTGCC
TCCGAGGCGGCGCGACGCGCTACGATATTGACCCTGGTCACCTCGGTGGCCTCATCCTACATCAATCTCCTGATCCTGGA
TCGGCAACTGGAGATCGCGCGGGCTACTGCAGCCACTCGAAGGGACACGCTGGAAGTGTTCCGGATGCGCTACGAAGGTG
GCGTGATTTCACAACTGGAGCTTGCTCAAATTGAGTCACAATATGAAACCGCAGTAGCCGCCATACCCCCGCTTGAATCC
AGCATCGCCCAGCAGGAGAACGCACTCGCAGTATTGCTCGGTCGCAATCCCGGTCCGATTGCACGGGGCCGCGAACTCGC
AAAGCTCGAACTTCCCATGGTATCCGCCGGTCTTCCATCGGAACTCCTGATGCGACGCCCTGATCTCCGTGAGGCCGAGC
AGAACCTGATCTCGGCCAATGCCCAAATCGGTGCTGCCCGTGCCCTTTACTTCCCGCGAATTTCACTCACAGGCGTGCTG
GGAGTCGCGAGCACCCAGCTTGCAACCCTGTTTACCGGACCAGCCTACATGGCAGCATTTGGCGTACTGACTTCGGTACC
GATCTTTACCGCAGGAGGCATCGCAGGCCAGGTGAAACAGGCGGAAGCACGGCACGAACAGGCACTCGTAGGTTACCAGC
GCGCAATCCAGATTGCGTTTCGGGAAGTTGAGGATTCCCTCATAGCCGTGCAGAAGAATCGAGCGGAACTGGCAGCTCAA
ACCCGGCGGGTGGAGGCGTCGCGCACGTATCTCGAGATGGCGCAGCTGCGTTATGACGAAGGCTATATCAGCTTCATCGA
AGTCCTTGATGCCCAACGCACCCTCTTCGAAGCTGAAACCACCCAGGCTCAAACCCGGGGACGGGTATTCAATTCTCTTG
TCGATCTCTATAAATCGCTCGGAGGTGGATGGGTGGTGGAGGCTGACCGTATCAGAGGACAAATGGAAACGGGCAGGCAG
GAAAACAAGTCCACACCCAAAGATGGAAACGTGATGGATGCAGCGGATCATGAACGAACAAAGACCGAACCTTGA

Upstream 100 bases:

>100_bases
CAACCAAAGCCGCGGAAAGCCAGCTACCCGCTGATCCAGGGGAAGGCAGGCAGGACGACTTGCCCAATGAAACCGTCCAA
TAAGTCCCCGGGAGACTGAC

Downstream 100 bases:

>100_bases
GTGTGTAAGCCTTCCTTTTGGCTTCAGTCACGATTCACCTCCAACTCCGGAGAAGACCAACACGGCTCTTTGCGAGACCC
ACTATGGAGACGAATACAAT

Product: RND efflux system outer membrane lipoprotein NodT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MRSRLTLLAFLLPLVLVLQSCMFGPNYRRPEIETMEKYRFGEKVARDIADTAWWEQFGDPILNELIRIALVENKDIRIAT
ARIEEFQGRYGATRSELFPQIGGDASAERLRSTAFVWPRGLGGIDPYYSYYQVSINANWEIDIWGRIRRLSEAARHQLFA
SEAARRATILTLVTSVASSYINLLILDRQLEIARATAATRRDTLEVFRMRYEGGVISQLELAQIESQYETAVAAIPPLES
SIAQQENALAVLLGRNPGPIARGRELAKLELPMVSAGLPSELLMRRPDLREAEQNLISANAQIGAARALYFPRISLTGVL
GVASTQLATLFTGPAYMAAFGVLTSVPIFTAGGIAGQVKQAEARHEQALVGYQRAIQIAFREVEDSLIAVQKNRAELAAQ
TRRVEASRTYLEMAQLRYDEGYISFIEVLDAQRTLFEAETTQAQTRGRVFNSLVDLYKSLGGGWVVEADRIRGQMETGRQ
ENKSTPKDGNVMDAADHERTKTEP

Sequences:

>Translated_504_residues
MRSRLTLLAFLLPLVLVLQSCMFGPNYRRPEIETMEKYRFGEKVARDIADTAWWEQFGDPILNELIRIALVENKDIRIAT
ARIEEFQGRYGATRSELFPQIGGDASAERLRSTAFVWPRGLGGIDPYYSYYQVSINANWEIDIWGRIRRLSEAARHQLFA
SEAARRATILTLVTSVASSYINLLILDRQLEIARATAATRRDTLEVFRMRYEGGVISQLELAQIESQYETAVAAIPPLES
SIAQQENALAVLLGRNPGPIARGRELAKLELPMVSAGLPSELLMRRPDLREAEQNLISANAQIGAARALYFPRISLTGVL
GVASTQLATLFTGPAYMAAFGVLTSVPIFTAGGIAGQVKQAEARHEQALVGYQRAIQIAFREVEDSLIAVQKNRAELAAQ
TRRVEASRTYLEMAQLRYDEGYISFIEVLDAQRTLFEAETTQAQTRGRVFNSLVDLYKSLGGGWVVEADRIRGQMETGRQ
ENKSTPKDGNVMDAADHERTKTEP
>Mature_504_residues
MRSRLTLLAFLLPLVLVLQSCMFGPNYRRPEIETMEKYRFGEKVARDIADTAWWEQFGDPILNELIRIALVENKDIRIAT
ARIEEFQGRYGATRSELFPQIGGDASAERLRSTAFVWPRGLGGIDPYYSYYQVSINANWEIDIWGRIRRLSEAARHQLFA
SEAARRATILTLVTSVASSYINLLILDRQLEIARATAATRRDTLEVFRMRYEGGVISQLELAQIESQYETAVAAIPPLES
SIAQQENALAVLLGRNPGPIARGRELAKLELPMVSAGLPSELLMRRPDLREAEQNLISANAQIGAARALYFPRISLTGVL
GVASTQLATLFTGPAYMAAFGVLTSVPIFTAGGIAGQVKQAEARHEQALVGYQRAIQIAFREVEDSLIAVQKNRAELAAQ
TRRVEASRTYLEMAQLRYDEGYISFIEVLDAQRTLFEAETTQAQTRGRVFNSLVDLYKSLGGGWVVEADRIRGQMETGRQ
ENKSTPKDGNVMDAADHERTKTEP

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=461, Percent_Identity=34.056399132321, Blast_Score=245, Evalue=5e-66,
Organism=Escherichia coli, GI2367348, Length=423, Percent_Identity=25.531914893617, Blast_Score=113, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 56236; Mature: 56236

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSRLTLLAFLLPLVLVLQSCMFGPNYRRPEIETMEKYRFGEKVARDIADTAWWEQFGDP
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
ILNELIRIALVENKDIRIATARIEEFQGRYGATRSELFPQIGGDASAERLRSTAFVWPRG
HHHHHHHHHHCCCCCEEEEHHHHHHHHCCCCCCHHHHCHHCCCCHHHHHHHHHHEECCCC
LGGIDPYYSYYQVSINANWEIDIWGRIRRLSEAARHQLFASEAARRATILTLVTSVASSY
CCCCCCCCEEEEEEEECCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
INLLILDRQLEIARATAATRRDTLEVFRMRYEGGVISQLELAQIESQYETAVAAIPPLES
HEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHH
SIAQQENALAVLLGRNPGPIARGRELAKLELPMVSAGLPSELLMRRPDLREAEQNLISAN
HHHHCCCCEEEEECCCCCCCCCCCCHHEEECCHHCCCCCHHHHHCCCCHHHHHHHHHHCC
AQIGAARALYFPRISLTGVLGVASTQLATLFTGPAYMAAFGVLTSVPIFTAGGIAGQVKQ
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHCCCCCHHHHHH
AEARHEQALVGYQRAIQIAFREVEDSLIAVQKNRAELAAQTRRVEASRTYLEMAQLRYDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GYISFIEVLDAQRTLFEAETTQAQTRGRVFNSLVDLYKSLGGGWVVEADRIRGQMETGRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCHHHCCCC
ENKSTPKDGNVMDAADHERTKTEP
CCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRSRLTLLAFLLPLVLVLQSCMFGPNYRRPEIETMEKYRFGEKVARDIADTAWWEQFGDP
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
ILNELIRIALVENKDIRIATARIEEFQGRYGATRSELFPQIGGDASAERLRSTAFVWPRG
HHHHHHHHHHCCCCCEEEEHHHHHHHHCCCCCCHHHHCHHCCCCHHHHHHHHHHEECCCC
LGGIDPYYSYYQVSINANWEIDIWGRIRRLSEAARHQLFASEAARRATILTLVTSVASSY
CCCCCCCCEEEEEEEECCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
INLLILDRQLEIARATAATRRDTLEVFRMRYEGGVISQLELAQIESQYETAVAAIPPLES
HEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHH
SIAQQENALAVLLGRNPGPIARGRELAKLELPMVSAGLPSELLMRRPDLREAEQNLISAN
HHHHCCCCEEEEECCCCCCCCCCCCHHEEECCHHCCCCCHHHHHCCCCHHHHHHHHHHCC
AQIGAARALYFPRISLTGVLGVASTQLATLFTGPAYMAAFGVLTSVPIFTAGGIAGQVKQ
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHCCCCCHHHHHH
AEARHEQALVGYQRAIQIAFREVEDSLIAVQKNRAELAAQTRRVEASRTYLEMAQLRYDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GYISFIEVLDAQRTLFEAETTQAQTRGRVFNSLVDLYKSLGGGWVVEADRIRGQMETGRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCHHHCCCC
ENKSTPKDGNVMDAADHERTKTEP
CCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]