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The map label for this gene is oprM [H]

Identifier: 82701823

GI number: 82701823

Start: 794331

End: 795755

Strand: Reverse

Name: oprM [H]

Synonym: Nmul_A0690

Alternate gene names: 82701823

Gene position: 795755-794331 (Counterclockwise)

Preceding gene: 82701824

Following gene: 82701822

Centisome position: 24.99

GC content: 57.61

Gene sequence:

>1425_bases
ATGAGAAACATCGTGACAAGCCGTCGTGACATGAGAGAGCGCCTGCGGAGCGGAATGGGGTTGTTGCTCTCCACTCTGCT
CCTTGCATCCTGCGCAATGGGGCCGGACTATATCCGCCCTCATGTCGAAACATCCGAAAGGTTTCGCCTGGCGCAAACGG
AGGGGCAATCCATTGCGAACCTGTCCTGGTGGGAATTGCTGCATGATAATCAACTGCAACAGCTCATCCGTCTCGCATTG
GCCCAGAACAAGGATCTCAAGCAGGCGGTAGCAAGCGTTGAAGAGCTGGAGGCACGCCTTGCTACTGCACGCATGGATTT
CCTTCCCAAAATGGACACAAATGCCAATGCGCCTGCTTTCGGAACCCTTGGAGGATTCAAGTTCCCTGGATTTCCCACAC
CTTACAGCTATTTCGGACAAGCGACCCTGAATTGGGAAATCGATATCTGGGGCCGCATTCGCCGTTCGAACGAAGCCGCC
CTGGCTGACTTGATGGCCCGGGAAGAAAACCGGCGCGCTGTGGTCCTGACCCTGGTAAGTTCGGTAGCACAGGCGTATTT
CGATCTCCTGCAATTCGATATGCAAATGGGTATTGCACGCAATGCCCTGCATTCATGGGAGGAGTCGGTGGCGCTTGCGC
GAGCCCAACTGCGGGGAGGGGTCATCTCCAGGCTGGATCTAGACCAGTTCGAAGCTGAGCGTGCCAATGCGGCCACCCGG
GTGGCAGAACTGCAACGGCTGACTGCCCAGAAGGAAAACGAACTGAGTGTATTGCTGGGAAGAAATCCGGTTTCGATAGA
ACGGGGACGCTCTCTCACGGAGCAGTTGCTGATACCAGAGGTGCCAGCCGGACTTCCTTCCGAACTGTTGCAGCGACGCC
CCGATATCCTGCAGGCGGAACAAACCCTTGCAGCAGCAACCGCCCGGATTGGGGCTGCCCAGGCGGCGCGATTTCCCAAG
CTCAATATCACCGGTTTCCTGGGCGTGGCAAGTCCGGCGCTGTCCAATCTCCTCCTTTCCGGGAGCGAGTTCGGGGTAGG
CGGACTGGGTCTGGCTGTTCCATTGATCAACGCGCAGGCTTTGGGAATGGAACAACGTGCCGCGGAAGCACAAGCCAGGC
AAATGCTTGCCCAGTACGAACAAACCATCCTGCTCGCATTCAGGGAAGTCGAGGATGCCCTGGTGGCGGTAAGCACCGCC
AACGAACAGCGCAAGGCGCAGGAACAGCAGGTGGATGCACTGCGCTCGGCTCTGCATATCGCTGATCTGCGCTACAAAGG
GGGTATTACCAGTTACGTGGATGTGCTACTGGCCAAACGGAATCTGTTCGATGCAGAGTTCGCGCTTGCGGCAACGCACC
GCCTCCATCTGATATCCGTGGTCCAACTATACAAAGCGCTCGGGGGGGGATGGACGCCCGTCTGA

Upstream 100 bases:

>100_bases
TTGCCACTTTTGTTGCCACAATATTTATTCCCTTGTTTTTTACCTGGCTTTCACGCAAGAATAAAGGTAAGCCAGTCGAA
AACCTGTCGCAGGAAACATC

Downstream 100 bases:

>100_bases
CGACATAGGATCAGTCCGGCAGATCTGAAAACTGATCTATCATAAAAATACTGCTTGTCATCACCCTCACTTAACCAGCG
GACCGCTACCCCTGGGTTTT

Product: RND efflux system outer membrane lipoprotein NodT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MRNIVTSRRDMRERLRSGMGLLLSTLLLASCAMGPDYIRPHVETSERFRLAQTEGQSIANLSWWELLHDNQLQQLIRLAL
AQNKDLKQAVASVEELEARLATARMDFLPKMDTNANAPAFGTLGGFKFPGFPTPYSYFGQATLNWEIDIWGRIRRSNEAA
LADLMAREENRRAVVLTLVSSVAQAYFDLLQFDMQMGIARNALHSWEESVALARAQLRGGVISRLDLDQFEAERANAATR
VAELQRLTAQKENELSVLLGRNPVSIERGRSLTEQLLIPEVPAGLPSELLQRRPDILQAEQTLAAATARIGAAQAARFPK
LNITGFLGVASPALSNLLLSGSEFGVGGLGLAVPLINAQALGMEQRAAEAQARQMLAQYEQTILLAFREVEDALVAVSTA
NEQRKAQEQQVDALRSALHIADLRYKGGITSYVDVLLAKRNLFDAEFALAATHRLHLISVVQLYKALGGGWTPV

Sequences:

>Translated_474_residues
MRNIVTSRRDMRERLRSGMGLLLSTLLLASCAMGPDYIRPHVETSERFRLAQTEGQSIANLSWWELLHDNQLQQLIRLAL
AQNKDLKQAVASVEELEARLATARMDFLPKMDTNANAPAFGTLGGFKFPGFPTPYSYFGQATLNWEIDIWGRIRRSNEAA
LADLMAREENRRAVVLTLVSSVAQAYFDLLQFDMQMGIARNALHSWEESVALARAQLRGGVISRLDLDQFEAERANAATR
VAELQRLTAQKENELSVLLGRNPVSIERGRSLTEQLLIPEVPAGLPSELLQRRPDILQAEQTLAAATARIGAAQAARFPK
LNITGFLGVASPALSNLLLSGSEFGVGGLGLAVPLINAQALGMEQRAAEAQARQMLAQYEQTILLAFREVEDALVAVSTA
NEQRKAQEQQVDALRSALHIADLRYKGGITSYVDVLLAKRNLFDAEFALAATHRLHLISVVQLYKALGGGWTPV
>Mature_474_residues
MRNIVTSRRDMRERLRSGMGLLLSTLLLASCAMGPDYIRPHVETSERFRLAQTEGQSIANLSWWELLHDNQLQQLIRLAL
AQNKDLKQAVASVEELEARLATARMDFLPKMDTNANAPAFGTLGGFKFPGFPTPYSYFGQATLNWEIDIWGRIRRSNEAA
LADLMAREENRRAVVLTLVSSVAQAYFDLLQFDMQMGIARNALHSWEESVALARAQLRGGVISRLDLDQFEAERANAATR
VAELQRLTAQKENELSVLLGRNPVSIERGRSLTEQLLIPEVPAGLPSELLQRRPDILQAEQTLAAATARIGAAQAARFPK
LNITGFLGVASPALSNLLLSGSEFGVGGLGLAVPLINAQALGMEQRAAEAQARQMLAQYEQTILLAFREVEDALVAVSTA
NEQRKAQEQQVDALRSALHIADLRYKGGITSYVDVLLAKRNLFDAEFALAATHRLHLISVVQLYKALGGGWTPV

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=453, Percent_Identity=33.3333333333333, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI2367348, Length=472, Percent_Identity=24.5762711864407, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 52204; Mature: 52204

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNIVTSRRDMRERLRSGMGLLLSTLLLASCAMGPDYIRPHVETSERFRLAQTEGQSIAN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHEEHHHCCCCCCCC
LSWWELLHDNQLQQLIRLALAQNKDLKQAVASVEELEARLATARMDFLPKMDTNANAPAF
CHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GTLGGFKFPGFPTPYSYFGQATLNWEIDIWGRIRRSNEAALADLMAREENRRAVVLTLVS
CCCCCCCCCCCCCCHHHHCCEEEEEEEEEHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHH
SVAQAYFDLLQFDMQMGIARNALHSWEESVALARAQLRGGVISRLDLDQFEAERANAATR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHH
VAELQRLTAQKENELSVLLGRNPVSIERGRSLTEQLLIPEVPAGLPSELLQRRPDILQAE
HHHHHHHHHCCCCCEEEEECCCCCCHHHCCHHHHHHCCCCCCCCCCHHHHHCCCHHHHHH
QTLAAATARIGAAQAARFPKLNITGFLGVASPALSNLLLSGSEFGVGGLGLAVPLINAQA
HHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
LGMEQRAAEAQARQMLAQYEQTILLAFREVEDALVAVSTANEQRKAQEQQVDALRSALHI
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ADLRYKGGITSYVDVLLAKRNLFDAEFALAATHRLHLISVVQLYKALGGGWTPV
HHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRNIVTSRRDMRERLRSGMGLLLSTLLLASCAMGPDYIRPHVETSERFRLAQTEGQSIAN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHEEHHHCCCCCCCC
LSWWELLHDNQLQQLIRLALAQNKDLKQAVASVEELEARLATARMDFLPKMDTNANAPAF
CHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GTLGGFKFPGFPTPYSYFGQATLNWEIDIWGRIRRSNEAALADLMAREENRRAVVLTLVS
CCCCCCCCCCCCCCHHHHCCEEEEEEEEEHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHH
SVAQAYFDLLQFDMQMGIARNALHSWEESVALARAQLRGGVISRLDLDQFEAERANAATR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHH
VAELQRLTAQKENELSVLLGRNPVSIERGRSLTEQLLIPEVPAGLPSELLQRRPDILQAE
HHHHHHHHHCCCCCEEEEECCCCCCHHHCCHHHHHHCCCCCCCCCCHHHHHCCCHHHHHH
QTLAAATARIGAAQAARFPKLNITGFLGVASPALSNLLLSGSEFGVGGLGLAVPLINAQA
HHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
LGMEQRAAEAQARQMLAQYEQTILLAFREVEDALVAVSTANEQRKAQEQQVDALRSALHI
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ADLRYKGGITSYVDVLLAKRNLFDAEFALAATHRLHLISVVQLYKALGGGWTPV
HHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]