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The map label for this gene is mexA [H]

Identifier: 82701822

GI number: 82701822

Start: 792971

End: 794206

Strand: Reverse

Name: mexA [H]

Synonym: Nmul_A0689

Alternate gene names: 82701822

Gene position: 794206-792971 (Counterclockwise)

Preceding gene: 82701823

Following gene: 82701821

Centisome position: 24.94

GC content: 56.72

Gene sequence:

>1236_bases
ATGAATCTGTCCCATCCGTGGCACCCTTTCAGCCGTTCATCTGCCGCCGCTGTCATCGCTCTAGGCACAGCCCTCATCCT
GATTTCTTTTTCCGGCTGCAAAAAAGCACCCCCTGCTCCAGCCCAGACTGTCCCCGAAGTGGAAGTGATTACCGTCACCA
CGCAGACTGTTCCCGACGAGCCTGAGTTTATCGGGCAAACACAGGCTTTTCGTCCCGTCGAGATTCGCCCGCAGGTAACG
GGAATAATCAAGAAAGTTTTTTTTACCGAGGGAACGAACGTCAAGAAGGGCAACAAGCTTTACCTGATCGACCCGGTGCC
CTTCGAGGCGGCCTATTTCAGCGGAAAGGCGAGAGTCGCGCAGGCCCAGGCCCGCCTCGTTCAGGCAGAGCAGGAAGAAG
CGCGCGTAAAACCTCTGCTAGAGGAACAGGCTGTCAGCCGCAAGAGTGTCGATGACGCAATCGCCGAAACGCTTGCCGCC
AAAGCAGGACTGGAGGCGGCGCAAAGCGACTTGATCAGGGCGAAATTCGATCTGGATAATACGATCATCACGGCCCCGGT
CAACGGGCGGATAGGACGCAGCCATTTCTATGAGGGGCGCCTGGTATCCGCCCAGACAACTCTGTTGACCACCATCGACC
AACTCGATCCCATGTACGTCAATGTCAATGTTCCCGAAAGCTATCTTCTCAGAAGGCGGCGTGAACTGGCGGAAAAGAAA
GTGCAAAGGCCGGATATCTTCCAGTTGCGGGGAGTGATCACTTTTTCCGATGGGAGCGTGTATCCGCAGGAAGGCGTGCT
CGATTTCTCCGACGTATCCATACGGCCGGAAACCGGTACGCTGGAGGGAAGATTCACGTTTCCGAATCCGGAAGGAAGCT
TTTCTCCCGGACAATCCTATTTTTATCCGGGGCAATTCGTTCATGTCCGCGTCAAGGGTTATATCCGGACAGATGCCATC
CTGATTCCGCAACGGGCTGTGCAACTGGGCCCCCAGGGCTCCTTCGTCTATGTCGTCGATGCCGAGAGCAAGGCGCAGTC
CAGACCCGTCACGGCCAGTTCCTGGCATGGCAAAGACTGGTTGATCGAAAACGGTCTTCGCCCCGGAGAGCAAGTAGTCG
TGGAAGGATTACACAGGATTCAGCCTGGAAGCCCGGTGAAGGCCATTCCGTACAAGGAGGAGCATCCAACGGGCCCCGGC
GAGCCCCAGAGTGAAGCGGGCGGGAAATCGGCATGA

Upstream 100 bases:

>100_bases
CTGAAAACTGATCTATCATAAAAATACTGCTTGTCATCACCCTCACTTAACCAGCGGACCGCTACCCCTGGGTTTTCAGG
GCAGCCCGCAGGAGACGTAC

Downstream 100 bases:

>100_bases
GTTCCCGCTTTTTTATCGATCGTCCAATTTTCGCATCCGTCCTGTCCATCATTATTGTGGTGGTGGGACTCGTTGCGCTG
AGAAACCTCCCAATTGCGCA

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MNLSHPWHPFSRSSAAAVIALGTALILISFSGCKKAPPAPAQTVPEVEVITVTTQTVPDEPEFIGQTQAFRPVEIRPQVT
GIIKKVFFTEGTNVKKGNKLYLIDPVPFEAAYFSGKARVAQAQARLVQAEQEEARVKPLLEEQAVSRKSVDDAIAETLAA
KAGLEAAQSDLIRAKFDLDNTIITAPVNGRIGRSHFYEGRLVSAQTTLLTTIDQLDPMYVNVNVPESYLLRRRRELAEKK
VQRPDIFQLRGVITFSDGSVYPQEGVLDFSDVSIRPETGTLEGRFTFPNPEGSFSPGQSYFYPGQFVHVRVKGYIRTDAI
LIPQRAVQLGPQGSFVYVVDAESKAQSRPVTASSWHGKDWLIENGLRPGEQVVVEGLHRIQPGSPVKAIPYKEEHPTGPG
EPQSEAGGKSA

Sequences:

>Translated_411_residues
MNLSHPWHPFSRSSAAAVIALGTALILISFSGCKKAPPAPAQTVPEVEVITVTTQTVPDEPEFIGQTQAFRPVEIRPQVT
GIIKKVFFTEGTNVKKGNKLYLIDPVPFEAAYFSGKARVAQAQARLVQAEQEEARVKPLLEEQAVSRKSVDDAIAETLAA
KAGLEAAQSDLIRAKFDLDNTIITAPVNGRIGRSHFYEGRLVSAQTTLLTTIDQLDPMYVNVNVPESYLLRRRRELAEKK
VQRPDIFQLRGVITFSDGSVYPQEGVLDFSDVSIRPETGTLEGRFTFPNPEGSFSPGQSYFYPGQFVHVRVKGYIRTDAI
LIPQRAVQLGPQGSFVYVVDAESKAQSRPVTASSWHGKDWLIENGLRPGEQVVVEGLHRIQPGSPVKAIPYKEEHPTGPG
EPQSEAGGKSA
>Mature_411_residues
MNLSHPWHPFSRSSAAAVIALGTALILISFSGCKKAPPAPAQTVPEVEVITVTTQTVPDEPEFIGQTQAFRPVEIRPQVT
GIIKKVFFTEGTNVKKGNKLYLIDPVPFEAAYFSGKARVAQAQARLVQAEQEEARVKPLLEEQAVSRKSVDDAIAETLAA
KAGLEAAQSDLIRAKFDLDNTIITAPVNGRIGRSHFYEGRLVSAQTTLLTTIDQLDPMYVNVNVPESYLLRRRRELAEKK
VQRPDIFQLRGVITFSDGSVYPQEGVLDFSDVSIRPETGTLEGRFTFPNPEGSFSPGQSYFYPGQFVHVRVKGYIRTDAI
LIPQRAVQLGPQGSFVYVVDAESKAQSRPVTASSWHGKDWLIENGLRPGEQVVVEGLHRIQPGSPVKAIPYKEEHPTGPG
EPQSEAGGKSA

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=391, Percent_Identity=38.1074168797954, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1789665, Length=371, Percent_Identity=37.466307277628, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI1789929, Length=375, Percent_Identity=34.4, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI87082036, Length=359, Percent_Identity=28.6908077994429, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789637, Length=159, Percent_Identity=32.0754716981132, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI87081951, Length=119, Percent_Identity=34.453781512605, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI87081796, Length=334, Percent_Identity=27.2455089820359, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 45027; Mature: 45027

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLSHPWHPFSRSSAAAVIALGTALILISFSGCKKAPPAPAQTVPEVEVITVTTQTVPDE
CCCCCCCCCCCCCCCHHHHHHHHEEEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCC
PEFIGQTQAFRPVEIRPQVTGIIKKVFFTEGTNVKKGNKLYLIDPVPFEAAYFSGKARVA
HHHCCCCCCCCCEECCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHEECCCHHHH
QAQARLVQAEQEEARVKPLLEEQAVSRKSVDDAIAETLAAKAGLEAAQSDLIRAKFDLDN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHEEEEECCCC
TIITAPVNGRIGRSHFYEGRLVSAQTTLLTTIDQLDPMYVNVNVPESYLLRRRRELAEKK
EEEEECCCCCCCCCCCCCCEEEECHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHH
VQRPDIFQLRGVITFSDGSVYPQEGVLDFSDVSIRPETGTLEGRFTFPNPEGSFSPGQSY
CCCCCCEEEEEEEEECCCCCCCCCCCEECCCCEECCCCCCEEEEEECCCCCCCCCCCCCE
FYPGQFVHVRVKGYIRTDAILIPQRAVQLGPQGSFVYVVDAESKAQSRPVTASSWHGKDW
ECCCCEEEEEEEEEEEECEEEECHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCH
LIENGLRPGEQVVVEGLHRIQPGSPVKAIPYKEEHPTGPGEPQSEAGGKSA
HHHCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNLSHPWHPFSRSSAAAVIALGTALILISFSGCKKAPPAPAQTVPEVEVITVTTQTVPDE
CCCCCCCCCCCCCCCHHHHHHHHEEEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCC
PEFIGQTQAFRPVEIRPQVTGIIKKVFFTEGTNVKKGNKLYLIDPVPFEAAYFSGKARVA
HHHCCCCCCCCCEECCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHEECCCHHHH
QAQARLVQAEQEEARVKPLLEEQAVSRKSVDDAIAETLAAKAGLEAAQSDLIRAKFDLDN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHEEEEECCCC
TIITAPVNGRIGRSHFYEGRLVSAQTTLLTTIDQLDPMYVNVNVPESYLLRRRRELAEKK
EEEEECCCCCCCCCCCCCCEEEECHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHH
VQRPDIFQLRGVITFSDGSVYPQEGVLDFSDVSIRPETGTLEGRFTFPNPEGSFSPGQSY
CCCCCCEEEEEEEEECCCCCCCCCCCEECCCCEECCCCCCEEEEEECCCCCCCCCCCCCE
FYPGQFVHVRVKGYIRTDAILIPQRAVQLGPQGSFVYVVDAESKAQSRPVTASSWHGKDW
ECCCCEEEEEEEEEEEECEEEECHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCH
LIENGLRPGEQVVVEGLHRIQPGSPVKAIPYKEEHPTGPGEPQSEAGGKSA
HHHCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]