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The map label for this gene is rnhB

Identifier: 82701801

GI number: 82701801

Start: 764134

End: 764733

Strand: Direct

Name: rnhB

Synonym: Nmul_A0668

Alternate gene names: 82701801

Gene position: 764134-764733 (Clockwise)

Preceding gene: 82701800

Following gene: 82701802

Centisome position: 24.0

GC content: 57.17

Gene sequence:

>600_bases
ATGTGCAGCAATGAATATCCTTGGGTCTGCGGTGTGGATGAGGCCGGCCGGGGGCCGCTGGCAGGACCGGTGTTCGCTGC
CTGCGTGATACTCGATCCGGCCTCTCCCATAGATGGCCTGGCAGATTCGAAAACGCTTACCGAGGGAAAGCGCAACAGCC
TGACCCTTGCCATAAAGGCGTATTCGATTGCCTGGGCCATCGGGTTCGCATCCGTCAGGGAAATAGACCGGCTGAATATA
TTGCAAGCAAGCCTGCTGGCGATGAAACGCGCGATCGGAAAATTGCCGGTGACGCCAGCGCGGGTATTAGTGGATGGAAA
CCACAGTCCCCGCCTGAAATTCCCCGTGACTACCATCGTGAGGGGCGACAGCCTGGTGCCGGAAATTTCCGCTGCCTCGA
TTCTTGCCAAGACCGCCCGCGATGCCGAGATGATGGCGTTACACCGGCGTTTTCCCCGTTATGGCTTCGATCAGCACAAA
GGATATTCCACGGAGCAGCATCTTGCCGCACTGAAAATTCACGGCGTTTCCATTGTGCACCGCCGCAGCTTTGCGCCGGT
AAGGGAACTCATGGCAAATGAAAAAGACGCGGAATCATGA

Upstream 100 bases:

>100_bases
GCGGAAGTGGACGGCCGCGTTGCCGCCGAGGCCGAACTGATGTGTACCGTCAGGCCAAAAGAGACCTGAAATACCTGCGG
AAAAACGGCAGAGTTGATCC

Downstream 100 bases:

>100_bases
ATAAATCCTGCCCTGAACCGGGCTTCCGCGAGGCTCCTGCTTCCGGCAATTTCCATTCCGCCTGACCGGCTGCCCCGCAA
AAAAGCCATATTCCATTTAA

Product: ribonuclease H

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MCSNEYPWVCGVDEAGRGPLAGPVFAACVILDPASPIDGLADSKTLTEGKRNSLTLAIKAYSIAWAIGFASVREIDRLNI
LQASLLAMKRAIGKLPVTPARVLVDGNHSPRLKFPVTTIVRGDSLVPEISAASILAKTARDAEMMALHRRFPRYGFDQHK
GYSTEQHLAALKIHGVSIVHRRSFAPVRELMANEKDAES

Sequences:

>Translated_199_residues
MCSNEYPWVCGVDEAGRGPLAGPVFAACVILDPASPIDGLADSKTLTEGKRNSLTLAIKAYSIAWAIGFASVREIDRLNI
LQASLLAMKRAIGKLPVTPARVLVDGNHSPRLKFPVTTIVRGDSLVPEISAASILAKTARDAEMMALHRRFPRYGFDQHK
GYSTEQHLAALKIHGVSIVHRRSFAPVRELMANEKDAES
>Mature_199_residues
MCSNEYPWVCGVDEAGRGPLAGPVFAACVILDPASPIDGLADSKTLTEGKRNSLTLAIKAYSIAWAIGFASVREIDRLNI
LQASLLAMKRAIGKLPVTPARVLVDGNHSPRLKFPVTTIVRGDSLVPEISAASILAKTARDAEMMALHRRFPRYGFDQHK
GYSTEQHLAALKIHGVSIVHRRSFAPVRELMANEKDAES

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=191, Percent_Identity=56.020942408377, Blast_Score=206, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_NITMU (Q2YB96)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411367.1
- ProteinModelPortal:   Q2YB96
- SMR:   Q2YB96
- STRING:   Q2YB96
- GeneID:   3785153
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0668
- eggNOG:   COG0164
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- PhylomeDB:   Q2YB96
- BioCyc:   NMUL323848:NMUL_A0668-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 21615; Mature: 21615

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSNEYPWVCGVDEAGRGPLAGPVFAACVILDPASPIDGLADSKTLTEGKRNSLTLAIKA
CCCCCCCEEECCCCCCCCCCCCHHHEEEEEECCCCCCCCCCCCCHHCCCCCCCEEEEEEE
YSIAWAIGFASVREIDRLNILQASLLAMKRAIGKLPVTPARVLVDGNHSPRLKFPVTTIV
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEECCEEEEE
RGDSLVPEISAASILAKTARDAEMMALHRRFPRYGFDQHKGYSTEQHLAALKIHGVSIVH
ECCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCHHHHHEEEEECCEEEEE
RRSFAPVRELMANEKDAES
CCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure
MCSNEYPWVCGVDEAGRGPLAGPVFAACVILDPASPIDGLADSKTLTEGKRNSLTLAIKA
CCCCCCCEEECCCCCCCCCCCCHHHEEEEEECCCCCCCCCCCCCHHCCCCCCCEEEEEEE
YSIAWAIGFASVREIDRLNILQASLLAMKRAIGKLPVTPARVLVDGNHSPRLKFPVTTIV
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEECCEEEEE
RGDSLVPEISAASILAKTARDAEMMALHRRFPRYGFDQHKGYSTEQHLAALKIHGVSIVH
ECCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCHHHHHEEEEECCEEEEE
RRSFAPVRELMANEKDAES
CCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA