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The map label for this gene is def [H]

Identifier: 82701784

GI number: 82701784

Start: 749408

End: 749941

Strand: Direct

Name: def [H]

Synonym: Nmul_A0651

Alternate gene names: 82701784

Gene position: 749408-749941 (Clockwise)

Preceding gene: 82701783

Following gene: 82701786

Centisome position: 23.53

GC content: 55.06

Gene sequence:

>534_bases
ATGCCGATTAAACCTGTCCTCAAAATGGGAGATCCCCGCCTACTGGAAGTGGCTCGGAAGGTGGAGAATTTCAGCACGCC
CGAATTCGGAGCATTGCTTCGAGACATGCATGATACGATGGAGGCATTGGAGGGCGCCGGCTTGGCTGCGCCGCAGATCG
GGATCGATCTGCAGGTTGTCATTTTCGGGGTGAAGCGTAATTTGCGCTATCCGGATGCGGAGGAGGTACCCTACACGGTA
TTGGTAAATCCGGTACTGACCCCTCTCACGGAATATATGGAGCAGGATTGGGAAGGTTGCCTGAGCGTTCCCGGCATGCG
CGGGATGGTTCCCCGATATGCCAGAATACGTTACGAGGGTTCGGATCAATATGGTAATCGCATAGACCGGACGGTGGAGG
GGTTTCACGCCCGGGTGGTGCAACACGAATGCGATCACTTGCAGGGAATTCTCTATCCGATGCGTATTACCGATTTTCGC
ACGTTCGGTTTTACCGATGTGCTGTTCCCGGACAGACCACCCTTGGCCGAGTAG

Upstream 100 bases:

>100_bases
AAAAGCAGTGCCCATCTCATCCGTCTGGATGAGGCGCATGGCGTTCTGGAAAAAACGATGGTCAGGGTCAGAAATATTCT
CGAATGGGTGGTAACGCTCG

Downstream 100 bases:

>100_bases
CAGTTTTCCAAACGACGGAAAGGGAAACGAACAGAACTTCCTACGGTTCTTATTTTATCCGGTGCGGGATGAGATCGTCA
GTTGCTGTGCCAGTGATTGA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 177; Mature: 176

Protein sequence:

>177_residues
MPIKPVLKMGDPRLLEVARKVENFSTPEFGALLRDMHDTMEALEGAGLAAPQIGIDLQVVIFGVKRNLRYPDAEEVPYTV
LVNPVLTPLTEYMEQDWEGCLSVPGMRGMVPRYARIRYEGSDQYGNRIDRTVEGFHARVVQHECDHLQGILYPMRITDFR
TFGFTDVLFPDRPPLAE

Sequences:

>Translated_177_residues
MPIKPVLKMGDPRLLEVARKVENFSTPEFGALLRDMHDTMEALEGAGLAAPQIGIDLQVVIFGVKRNLRYPDAEEVPYTV
LVNPVLTPLTEYMEQDWEGCLSVPGMRGMVPRYARIRYEGSDQYGNRIDRTVEGFHARVVQHECDHLQGILYPMRITDFR
TFGFTDVLFPDRPPLAE
>Mature_176_residues
PIKPVLKMGDPRLLEVARKVENFSTPEFGALLRDMHDTMEALEGAGLAAPQIGIDLQVVIFGVKRNLRYPDAEEVPYTVL
VNPVLTPLTEYMEQDWEGCLSVPGMRGMVPRYARIRYEGSDQYGNRIDRTVEGFHARVVQHECDHLQGILYPMRITDFRT
FGFTDVLFPDRPPLAE

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=174, Percent_Identity=35.0574712643678, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI1789682, Length=157, Percent_Identity=34.3949044585987, Blast_Score=82, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24645726, Length=176, Percent_Identity=35.7954545454545, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24645728, Length=173, Percent_Identity=31.2138728323699, Blast_Score=88, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 20151; Mature: 20019

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIKPVLKMGDPRLLEVARKVENFSTPEFGALLRDMHDTMEALEGAGLAAPQIGIDLQVV
CCCCCCHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
IFGVKRNLRYPDAEEVPYTVLVNPVLTPLTEYMEQDWEGCLSVPGMRGMVPRYARIRYEG
EEECHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEEEECC
SDQYGNRIDRTVEGFHARVVQHECDHLQGILYPMRITDFRTFGFTDVLFPDRPPLAE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCEECCCCEECCCCCCCCCCC
>Mature Secondary Structure 
PIKPVLKMGDPRLLEVARKVENFSTPEFGALLRDMHDTMEALEGAGLAAPQIGIDLQVV
CCCCCHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
IFGVKRNLRYPDAEEVPYTVLVNPVLTPLTEYMEQDWEGCLSVPGMRGMVPRYARIRYEG
EEECHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEEEECC
SDQYGNRIDRTVEGFHARVVQHECDHLQGILYPMRITDFRTFGFTDVLFPDRPPLAE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCEECCCCEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]