Click here to switch to the map view.

The map label for this gene is queF

Identifier: 82701777

GI number: 82701777

Start: 739575

End: 739994

Strand: Reverse

Name: queF

Synonym: Nmul_A0644

Alternate gene names: 82701777

Gene position: 739994-739575 (Counterclockwise)

Preceding gene: 82701785

Following gene: 82701774

Centisome position: 23.24

GC content: 53.33

Gene sequence:

>420_bases
ATGCCCAGCCGCCCGGATAAAAACCTGGAAACCTTCCCCAATCCTACTCAGGAACGGGACTACCATATTCATATGGAGAT
CCCCGAGTTTACCTGCTTGTGCCCCAAAACCGGCCAGCCCGATTTCGCGACCCTGATTCTTGATTATATTCCTGACAAGA
AGTGCGTCGAACTTAAAAGCCTCAAGCTCTATATCTGGTCTTTCCGTGACGAGAACGCTTTCCACGAAGCGGTGACAAAC
CGGATTGTCGATGACTTGGCTACAGCCCTGCAGCCTCGTTATCTACGTCTCACCGCAAAATTCTACGTGCGTGGCGGCAT
TTTCACCACGGTCGTTGCAGAGCACCGTCATTCCGGTTGGACTCCCCTGCCCTCCGTAGATCTCTTCCATTTCGATAGCC
AGCCCTCCACGCGCGGATGA

Upstream 100 bases:

>100_bases
GCCTCCTGGATGAAGCTTCCGGTTCGGGAACATTCCGAATCATCCTGGGAGAGGCCTCCCAAGGGAGCTCATTCTAACCG
ATTTTGACAGAGATAAGAGA

Downstream 100 bases:

>100_bases
GTCTGCCATCCGCCACCGAATGCAGCCCCTGTTCAGCGAATGACGCCCATCCCATGCCGGCCGGCAATTTACCTGAAACA
TGCGGATGAAATAATCGCTG

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 139; Mature: 138

Protein sequence:

>139_residues
MPSRPDKNLETFPNPTQERDYHIHMEIPEFTCLCPKTGQPDFATLILDYIPDKKCVELKSLKLYIWSFRDENAFHEAVTN
RIVDDLATALQPRYLRLTAKFYVRGGIFTTVVAEHRHSGWTPLPSVDLFHFDSQPSTRG

Sequences:

>Translated_139_residues
MPSRPDKNLETFPNPTQERDYHIHMEIPEFTCLCPKTGQPDFATLILDYIPDKKCVELKSLKLYIWSFRDENAFHEAVTN
RIVDDLATALQPRYLRLTAKFYVRGGIFTTVVAEHRHSGWTPLPSVDLFHFDSQPSTRG
>Mature_138_residues
PSRPDKNLETFPNPTQERDYHIHMEIPEFTCLCPKTGQPDFATLILDYIPDKKCVELKSLKLYIWSFRDENAFHEAVTNR
IVDDLATALQPRYLRLTAKFYVRGGIFTTVVAEHRHSGWTPLPSVDLFHFDSQPSTRG

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_NITMU (Q2YBC0)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411343.1
- ProteinModelPortal:   Q2YBC0
- SMR:   Q2YBC0
- STRING:   Q2YBC0
- GeneID:   3785417
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0644
- eggNOG:   COG0780
- HOGENOM:   HBG294103
- OMA:   FRNAGIF
- BioCyc:   NMUL323848:NMUL_A0644-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00818
- InterPro:   IPR016856
- InterPro:   IPR020602
- PIRSF:   PIRSF027377
- TIGRFAMs:   TIGR03139

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 16109; Mature: 15978

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSRPDKNLETFPNPTQERDYHIHMEIPEFTCLCPKTGQPDFATLILDYIPDKKCVELKS
CCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEECCCCCCCHHHHHHHHHCCCCHHEEEEE
LKLYIWSFRDENAFHEAVTNRIVDDLATALQPRYLRLTAKFYVRGGIFTTVVAEHRHSGW
EEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHHHCCCCC
TPLPSVDLFHFDSQPSTRG
CCCCCCEEEEECCCCCCCC
>Mature Secondary Structure 
PSRPDKNLETFPNPTQERDYHIHMEIPEFTCLCPKTGQPDFATLILDYIPDKKCVELKS
CCCCCCCCCCCCCCCCCCCEEEEEECCCEEEECCCCCCCHHHHHHHHHCCCCHHEEEEE
LKLYIWSFRDENAFHEAVTNRIVDDLATALQPRYLRLTAKFYVRGGIFTTVVAEHRHSGW
EEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHHHCCCCC
TPLPSVDLFHFDSQPSTRG
CCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA