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The map label for this gene is dosC [H]

Identifier: 82701771

GI number: 82701771

Start: 728537

End: 730246

Strand: Reverse

Name: dosC [H]

Synonym: Nmul_A0638

Alternate gene names: 82701771

Gene position: 730246-728537 (Counterclockwise)

Preceding gene: 82701772

Following gene: 82701769

Centisome position: 22.93

GC content: 56.49

Gene sequence:

>1710_bases
ATGGCCCTTCTAAAGAGCATTAAGAGCAAACTCGTGGTGTTCGCCACCGTGGCTAGCCTGATTCCGTCGCTGGGGCTCGG
ATTAAAGAGTATTAAGAGCAAACTCGTGGTGTTCGCCACCCTGGCTACGCTGATCCCGTCGCTGGGGCTCGGATTATTGA
CATTCAGGCAGAGTGAAGCGCAGACCAATGCCAACGTCACGCGCGAGCTTCGCGCGCTCACGGATTATGCGGGCCGGGAA
ATGGAATTCTGGCTCGACAGGCGTGCACATGAAGCGCACGTTACCGCAGCATCCGGAGCGGTCATCGAAGCATTATCCGC
ATCTTATCAACGCCGCGCGGATATGCTGGCCAAATACCCAGGAGCCCTCGCGCGTTACCTGCAGTCTGTTCAGGGGAAGC
TTGATGCGATACGGGAATTGACCGTGATGAACGCGTCCGGAAAAATTATCGCGAGCAGTGCCGAAAAACCCGCGGGAGCA
ACGCTCCCCGACGAGTGGCCGCAAAACTCGGTCACGGAAGGTCTTGTGATCACACCCCCTCACTGGAACGAGCAGTACAA
CACCGCGACCCTCAGCGTCGCGGTTCCGGTGTTGTCATACGACAACGTGTTGATGGGGGCGCTGGTAGCCGTGCTTGACC
TGCGCAACCTGCAGCCCCATTTGAAGAGTTCTTCGACATCACCCCCGGGCGAGGTATTTCTGCTGGATCCGGATGGCCGT
GTGCTCATCGGCACCTTGGCGGAAGGAGAAACCATGGCGCAACTCGATGGCCCCTCGTTGCGCGAGCTTCTCGCACGCCC
CGGCGAATCCATGTTCTTCAAGGGATTGACACGCCGTGAAGTAATCGGTCTGGCAGATGTATTCCATGAATTCCCCCTTA
CCATTCTGGCGGAGCGTGATCGGGCCGCCGTCTATGGCGACTGGATCGAACTGCGCAATATGCTCCTGATACTGGTCGGT
ATGCTGGTTCTAATCGTAGCCGCCGCGGCATTCCAAATGGGACGCTCGATCGTCGTGCCGCTGCAACGTCTTATGCGTGC
AGCCGACCGGATCGCGGATGGCGACCTGGAAGTGCGGCTTTCCGCAACGCGGAATGACGAGATCGGCCACCTCACGAAGG
TATTTGATCAGATGGCAGACCGATTGCGACGGAACCACGCCGAAATCATGGCTGCGCATGAAGCCATGCAACAACAAAAC
CGGATGCTTGAAAAACTCTCGATTACCGATAGCCTCACCGGCCTCTATAATCGCAACAAGCTTGATGCCATCCTGGCCGA
TCAACTGGCGCGATTCAGACGCACGCAACGGCCTTTTGCACTGCTAATGCTGGATATCGACCACTTCAAGACACTCAATG
ACACATACGGCCATATTACCGGCGACAAAATTCTGGCCAAGGTTGCGCAAATCCTTCTCCAGTCGATCCGCAGTATTGAT
TATGCGGCGCGCTACGGGGGCGATGAATTTATCATCATCCTGGTCGAAACCTACATCGATCAGGCTGTCAAAACCGCTGA
GCGCATCCGCACGCATGTGGAAAAACTGACCTATCCAGCCGGAAAATCGACGATATCGATCACGGTGAGTATCGGGGCGG
TGGAGTGCCGCTCTGACGACAGCACAACGACAGCAGTATTCTCCCGCGCCGACAATGCACTTTATGAAGCCAAGCGTGCT
GGCCGCAATCAGGCATACTTCTCCCGCTAA

Upstream 100 bases:

>100_bases
GCGATCTGCAGCCCTTATTTCAAGCTGTTTCTCGGAATAATTCTGCTGAAATTCTGACAGTATTTATCCGTTGAGTAACC
TTTATCCGGAACTCCGTCCC

Downstream 100 bases:

>100_bases
ATCCTATTTGACACCCATATCGATTCCTTTGGCCACCTGCGCCTCCACCAGCGTACTGAATATCCATCCCGAGCGACCAT
CTGCGGTAAGAATTTGCAAC

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 569; Mature: 568

Protein sequence:

>569_residues
MALLKSIKSKLVVFATVASLIPSLGLGLKSIKSKLVVFATLATLIPSLGLGLLTFRQSEAQTNANVTRELRALTDYAGRE
MEFWLDRRAHEAHVTAASGAVIEALSASYQRRADMLAKYPGALARYLQSVQGKLDAIRELTVMNASGKIIASSAEKPAGA
TLPDEWPQNSVTEGLVITPPHWNEQYNTATLSVAVPVLSYDNVLMGALVAVLDLRNLQPHLKSSSTSPPGEVFLLDPDGR
VLIGTLAEGETMAQLDGPSLRELLARPGESMFFKGLTRREVIGLADVFHEFPLTILAERDRAAVYGDWIELRNMLLILVG
MLVLIVAAAAFQMGRSIVVPLQRLMRAADRIADGDLEVRLSATRNDEIGHLTKVFDQMADRLRRNHAEIMAAHEAMQQQN
RMLEKLSITDSLTGLYNRNKLDAILADQLARFRRTQRPFALLMLDIDHFKTLNDTYGHITGDKILAKVAQILLQSIRSID
YAARYGGDEFIIILVETYIDQAVKTAERIRTHVEKLTYPAGKSTISITVSIGAVECRSDDSTTTAVFSRADNALYEAKRA
GRNQAYFSR

Sequences:

>Translated_569_residues
MALLKSIKSKLVVFATVASLIPSLGLGLKSIKSKLVVFATLATLIPSLGLGLLTFRQSEAQTNANVTRELRALTDYAGRE
MEFWLDRRAHEAHVTAASGAVIEALSASYQRRADMLAKYPGALARYLQSVQGKLDAIRELTVMNASGKIIASSAEKPAGA
TLPDEWPQNSVTEGLVITPPHWNEQYNTATLSVAVPVLSYDNVLMGALVAVLDLRNLQPHLKSSSTSPPGEVFLLDPDGR
VLIGTLAEGETMAQLDGPSLRELLARPGESMFFKGLTRREVIGLADVFHEFPLTILAERDRAAVYGDWIELRNMLLILVG
MLVLIVAAAAFQMGRSIVVPLQRLMRAADRIADGDLEVRLSATRNDEIGHLTKVFDQMADRLRRNHAEIMAAHEAMQQQN
RMLEKLSITDSLTGLYNRNKLDAILADQLARFRRTQRPFALLMLDIDHFKTLNDTYGHITGDKILAKVAQILLQSIRSID
YAARYGGDEFIIILVETYIDQAVKTAERIRTHVEKLTYPAGKSTISITVSIGAVECRSDDSTTTAVFSRADNALYEAKRA
GRNQAYFSR
>Mature_568_residues
ALLKSIKSKLVVFATVASLIPSLGLGLKSIKSKLVVFATLATLIPSLGLGLLTFRQSEAQTNANVTRELRALTDYAGREM
EFWLDRRAHEAHVTAASGAVIEALSASYQRRADMLAKYPGALARYLQSVQGKLDAIRELTVMNASGKIIASSAEKPAGAT
LPDEWPQNSVTEGLVITPPHWNEQYNTATLSVAVPVLSYDNVLMGALVAVLDLRNLQPHLKSSSTSPPGEVFLLDPDGRV
LIGTLAEGETMAQLDGPSLRELLARPGESMFFKGLTRREVIGLADVFHEFPLTILAERDRAAVYGDWIELRNMLLILVGM
LVLIVAAAAFQMGRSIVVPLQRLMRAADRIADGDLEVRLSATRNDEIGHLTKVFDQMADRLRRNHAEIMAAHEAMQQQNR
MLEKLSITDSLTGLYNRNKLDAILADQLARFRRTQRPFALLMLDIDHFKTLNDTYGHITGDKILAKVAQILLQSIRSIDY
AARYGGDEFIIILVETYIDQAVKTAERIRTHVEKLTYPAGKSTISITVSIGAVECRSDDSTTTAVFSRADNALYEAKRAG
RNQAYFSR

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=156, Percent_Identity=45.5128205128205, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1787262, Length=167, Percent_Identity=40.1197604790419, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI1786584, Length=169, Percent_Identity=33.7278106508876, Blast_Score=115, Evalue=9e-27,
Organism=Escherichia coli, GI87082007, Length=162, Percent_Identity=40.7407407407407, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI87081881, Length=174, Percent_Identity=35.0574712643678, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1788085, Length=174, Percent_Identity=35.0574712643678, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1787816, Length=163, Percent_Identity=38.0368098159509, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787541, Length=184, Percent_Identity=30.9782608695652, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1787802, Length=159, Percent_Identity=34.5911949685535, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788381, Length=170, Percent_Identity=32.3529411764706, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI87081974, Length=153, Percent_Identity=35.2941176470588, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1788956, Length=170, Percent_Identity=31.1764705882353, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87081977, Length=166, Percent_Identity=27.710843373494, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1787056, Length=126, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 62701; Mature: 62570

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: PS50885 HAMP ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLKSIKSKLVVFATVASLIPSLGLGLKSIKSKLVVFATLATLIPSLGLGLLTFRQSEA
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHEEHHHH
QTNANVTRELRALTDYAGREMEFWLDRRAHEAHVTAASGAVIEALSASYQRRADMLAKYP
HCCHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHEEHHCCHHHHHHHHHHHHHHHHHHHCH
GALARYLQSVQGKLDAIRELTVMNASGKIIASSAEKPAGATLPDEWPQNSVTEGLVITPP
HHHHHHHHHHHHHHHHHHHHHEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCCEEECCC
HWNEQYNTATLSVAVPVLSYDNVLMGALVAVLDLRNLQPHLKSSSTSPPGEVFLLDPDGR
CCCCCCCCEEEEEEEEHHCCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCCEEEEECCCCC
VLIGTLAEGETMAQLDGPSLRELLARPGESMFFKGLTRREVIGLADVFHEFPLTILAERD
EEEEEECCCCEEECCCCHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHCCCEEEECCC
RAAVYGDWIELRNMLLILVGMLVLIVAAAAFQMGRSIVVPLQRLMRAADRIADGDLEVRL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEE
SATRNDEIGHLTKVFDQMADRLRRNHAEIMAAHEAMQQQNRMLEKLSITDSLTGLYNRNK
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
LDAILADQLARFRRTQRPFALLMLDIDHFKTLNDTYGHITGDKILAKVAQILLQSIRSID
HHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YAARYGGDEFIIILVETYIDQAVKTAERIRTHVEKLTYPAGKSTISITVSIGAVECRSDD
HHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEECCCC
STTTAVFSRADNALYEAKRAGRNQAYFSR
CHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ALLKSIKSKLVVFATVASLIPSLGLGLKSIKSKLVVFATLATLIPSLGLGLLTFRQSEA
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHEEHHHH
QTNANVTRELRALTDYAGREMEFWLDRRAHEAHVTAASGAVIEALSASYQRRADMLAKYP
HCCHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHEEHHCCHHHHHHHHHHHHHHHHHHHCH
GALARYLQSVQGKLDAIRELTVMNASGKIIASSAEKPAGATLPDEWPQNSVTEGLVITPP
HHHHHHHHHHHHHHHHHHHHHEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCCEEECCC
HWNEQYNTATLSVAVPVLSYDNVLMGALVAVLDLRNLQPHLKSSSTSPPGEVFLLDPDGR
CCCCCCCCEEEEEEEEHHCCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCCEEEEECCCCC
VLIGTLAEGETMAQLDGPSLRELLARPGESMFFKGLTRREVIGLADVFHEFPLTILAERD
EEEEEECCCCEEECCCCHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHCCCEEEECCC
RAAVYGDWIELRNMLLILVGMLVLIVAAAAFQMGRSIVVPLQRLMRAADRIADGDLEVRL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEE
SATRNDEIGHLTKVFDQMADRLRRNHAEIMAAHEAMQQQNRMLEKLSITDSLTGLYNRNK
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
LDAILADQLARFRRTQRPFALLMLDIDHFKTLNDTYGHITGDKILAKVAQILLQSIRSID
HHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YAARYGGDEFIIILVETYIDQAVKTAERIRTHVEKLTYPAGKSTISITVSIGAVECRSDD
HHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEECCCC
STTTAVFSRADNALYEAKRAGRNQAYFSR
CHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]